Artificial Red Cells : Physiological Importance of the Ligand Reaction Dynamism and the New Application for Ligand Therapy
| Project/Area Number |
19300164
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Waseda University |
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Principal Investigator |
SAKAI Hiromi Waseda University, 理工学術院, 准教授 (70318830)
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| Co-Investigator(Kenkyū-buntansha) |
KOBAYASHI Koichi 慶應義塾大学, 医学部 (80051704)
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| Project Period (FY) |
2007 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥19,240,000 (Direct Cost: ¥14,800,000、Indirect Cost: ¥4,440,000)
Fiscal Year 2009: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2008: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2007: ¥12,870,000 (Direct Cost: ¥9,900,000、Indirect Cost: ¥2,970,000)
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| Keywords | 人工臓器工学 / 人工赤血球 / 生体材料 / 生物・生体工学 / ナノバイオ / 生体機能利用 / 人工血液 / 一酸化窒素 / 一酸化炭素 / レオロジー |
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| Research Abstract |
Chemically modified hemoglobin (Hb) solutions as artificial O_2 carriers show vasoconstriction and hypertension presumably due to the high affinity for endogenous NO and CO as vasorelaxation factors, and due to the faster O_2-release that induces autoregulatory vasoconstriction. On the other hand, cellular-type Hb-vesicle (HbV) does not induce such side effects. Herein we analyzed the reactions of ligand gaseous molecules by physicochemical techniques, and found out that HbV shows much slower reactions and it is due to the intracellular gas diffusion barrier induced by a concentrated Hb solution, and the retarded lateral diffusion of HbV when it flows in a narrow tube. In addition, we tested HbV as an exogenous CO carrier because endogenous CO is cytoprotective. After inducing hemorrhagic shock, rats were resuscitated with CO-bound HbV. It showed cytoprotective effect at reperfusion, and CO was dissociated rapidly and exhaled through the lung. No symptom related to toxicity of CO was detected. The results imply the possibility of artificial red cells for a new ligand therapy.
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Report
(4 results)
Research Products
(72 results)
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[Journal Article] The role of luminal factors in the recovery of gastric functions and behavioral changes after chronic Helicobacter pylori infection.2008
Author(s)
E.F. Verdu, P. Bercik, X.X. Huang, J. Lu, N. Al-Mutawaly, H. Sakai, T.A. Tompkins, K. Croitoru, E. Tsuchida, M. Prude, S.M. Collins.
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Journal Title
Am. J. Physiol. Gastrointest. Liver Physiol. 295
Related Report
Peer Reviewed
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[Presentation]2007
Author(s)
酒井 宏水、土田 英俊
Organizer
第4回Heme Oxygenase研究フォーラム
Place of Presentation
京都府立医科大学
Year and Date
2007-08-24
Related Report
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