Modulation of cellular metabolism based on controlling the mobility of multivalent ligands using stimuli-responsive polyrotaxanes

• Project/Area Number: 19300170
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biomedical engineering/Biological material science
• Research Institution: Japan Advanced Institute of Science and Technology
• Principal Investigator: YUI Nobuhiko Japan Advanced Institute of Science and Technology, 生体材料工学研究所, 教授 (70182665)
• Co-Investigator(Kenkyū-buntansha): 上遠野 亮 北陸先端科学技術大学院大学, マテリアルサイエンス研究科, 助教 (60432142)
• Project Period (FY): 2007 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2008: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2007: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
• Keywords: 細胞工学 / 生体機能材料 / リガンド導入ポリロタキサン / 多価相互作用 / クラスタリング / 細胞・組織工学

Research Abstract

In order to aim at controlling cellular response via stimuli-responsive polyrotaxanes, the preparation of a variety of polyrotaxanes, the characterization of their properties, and the protein and cellular responses to these polyrotaxanes were performed. Macromolecular inclusion of loose-fit polyrotaxane, temperature-responsive polyrotaxane formation, enhanced binding of mannose-conjugated polyrotaxane with specific protein, and the production of anti-inflammatory cytokine from macrophages were clarified in this study.

Research Products

• [Journal Article] Ligand accessibility to receptor binding sites enhanced by movable polyrotaxanes, Macromolecular Bioscience (2011)
  • Author(s): H.Hyun, N.Yui
  • Journal Title: published online 11
  • Peer Reviewed
• [Journal Article] Anti-inflammatory response of mannose-conjugated polyrotaxane endocytosed by macrophage (2011)
  • Author(s): T.Ehashi, H.Hyun, N.Yui
  • Journal Title: Macromolecular Research 19 in press
  • Peer Reviewed
• [Journal Article] Azidated polyrotaxanes for facile and efficient functionalization via click chemistry (2011)
  • Author(s): H.Hyun, N.Yui
  • Journal Title: Macromolecular Rapid Communications 32 Pages: 326-221
  • Peer Reviewed
• [Journal Article] Heat-induced supramolecular crosslinking of dumbbell-shaped PEG with β-CD dimer based on reversible loose-fit rotaxanation (2011)
  • Author(s): R.Katoono, Y.Kobayashi, M.Yamaguchi, N.Yui
  • Journal Title: Macromolecuclar Chemistry and Physics Volume: 212 Pages: 211-215
  • Peer Reviewed
• [Journal Article] Azidated polyrotaxanes for facile and efficient functionalization via clickchemistry (2011)
  • Author(s): H.Hyun, N.Yui
  • Journal Title: Macromolecuclar Rapid Communications Volume: 32 Pages: 326-331
  • Peer Reviewed
• [Journal Article] Anti-inflammatory response of mannose-conjugated polyrotaxane endocytosed by macrophage (2011)
  • Author(s): T.Ehashi, H.Hyun, N.Yui
  • Journal Title: Macromolecuclar Research Volume: 印刷中
  • Peer Reviewed
• [Journal Article] Ligand Accessibility to Receptor Binding Sites Enhanced by MovablePolyrotaxanes (2011)
  • Author(s): H.Hyun, N.Yui
  • Journal Title: Macromolecuclar Bioscience Volume: 11(印刷中)
  • Peer Reviewed
• [Journal Article] Preparation of nano-aggregates through self-assembly of amphiphilic polyrotaxane composed of PLLA-PEG-PLLA triblock copolymer and α-cyclodextrin (2010)
  • Author(s): K.Nagahama, J.Ohmura, H.Sakaue, T.Ouchi, Y.Ohya, N.Yui
  • Journal Title: Chemistry Letter 39 Pages: 250-251
  • NAID: 10027178354
  • Peer Reviewed
• [Journal Article] Intranuclear DNA release is a determinant of transfection activity for anon-viral vector : biocleavable polyrotaxane as a supramolecularly dissociative condenser for efficient intracellular DNA release (2010)
  • Author(s): Y.Yamada, T.Nomura, H.Harashima, A.Yamashita, R.Katoono, N.Yui
  • Journal Title: Biological and Pharmaceutical Bulletin Volume: 33 Pages: 1218-1222
  • Peer Reviewed
• [Journal Article] Preparation of loose-fit polyrotaxane composed of f3-cyclodextrin and poly(ethylene glycol) derivatives through the slipping-expanding protocol (2010)
  • Author(s): R.Katoono, Y.Kobayashi, N.Yui
  • Journal Title: Chemistry Letter Volume: 39 Pages: 892-893
  • Peer Reviewed
• [Journal Article] Supramolecular surfaces modulating cellular response (2010)
  • Author(s): N.Yui
  • Journal Title: Advances in Science and Technology Volume: 76 Pages: 10-15
  • Peer Reviewed
• [Journal Article] Preparation of nano-aggregates through self-assembly of amphiphilic polyrotaxane composed of PLLA-PEG-PLLA triblock copolymer and α-cyclodextrin (2010)
  • Author(s): K. Nagahama, J. Ohmura, H. Sakaue, T. Ouchi, Y. Ohya, N. Yui
  • Journal Title: Chemistry Letter 39 Pages: 250-251
  • NAID: 10027178354
  • Peer Reviewed
• [Journal Article] Preparation of nano-aggregates through self-assembly of amphiphilic polyrotaxane composed of PLLA-PEG-PLLA triblock copolymer and α-cyclodextrin (2010)
  • Author(s): K.Nagahama, J.Ohmura, H.Sakaue, T.Ouchi, Y.Ohya, 由井伸彦
  • Journal Title: Chemistry Letter 39 Pages: 250-251
  • NAID: 10027178354
  • Peer Reviewed
• [Journal Article] Functional cyclodextrins polyrotaxanes for drug delivery (2009)
  • Author(s): N.Yui, R.Katoono, A.Yamashita
  • Journal Title: Advances in Polymer Sciences 222 Pages: 55-77
  • Peer Reviewed
• [Journal Article] Supramolecular mobility in polyrotaxanes exploits biomedical functions (2009)
  • Author(s): N.Yui
  • Journal Title: Macromolecular Symposia 279 Pages: 158-162
  • Peer Reviewed
• [Journal Article] Polyrotaxane composed of poly-L-lactide and alpha-cyclodextrin exhibiting protease-triggering hydrolysis (2009)
  • Author(s): Y.Ohya, S.Takamido, K.Nagahama, T.Ouchi, R.Katoono, N.Yui
  • Journal Title: Biomacromolecules 10 Pages: 2261-2267
  • Peer Reviewed
• [Journal Article] Loose-Fit Polyrotaxane Composed of γ-CD and Single PEG Chain : Making Room in γ-CD Cavity for Additional Inclusion Complexation (2009)
  • Author(s): A.Takahashi, R.Katoono, N.Yui
  • Journal Title: Macromolecules 42 Pages: 8587-9589
  • Peer Reviewed
• [Journal Article] Functional cyclodextrins polyrotaxanes for drug delivery (2009)
  • Author(s): N. Yui, R. Katoono, A. Yamashita
  • Journal Title: Advances in Polymer Sciences 222 Pages: 55-77
  • Peer Reviewed
• [Journal Article] Supramolecular mobility in polyrotaxanes exploits biomedical functions (2009)
  • Author(s): N. Yui
  • Journal Title: Macromolecular Symposia 279 Pages: 158-162
  • Peer Reviewed
• [Journal Article] Polyrotaxane composed of poly-L-lactide and alpha-cyclodextrin exhibiting protease-triggering hydrolysis (2009)
  • Author(s): Y. Ohya, S. Takamido, K. Nagahama, T. Ouchi, R. Katoono, N. Yui
  • Journal Title: Biomacromolecules 10 Pages: 2261-2267
  • Peer Reviewed
• [Journal Article] Loose-Fit Polyrotaxane Composed of γ-CD and Single PEG Chain : Making Room in γ-CD Cavity for Additional Inclusion Complexation (2009)
  • Author(s): A. Takahashi, R. Katoono, N. Yui
  • Journal Title: Macromolecules 42 Pages: 8587-8589
  • Peer Reviewed
• [Journal Article] ポリロタキサンの動的特性を活かしたバイオマテリアル機能設計 (2009)
  • Author(s): 由井伸彦
  • Journal Title: 高分子 58 Pages: 678-681
  • NAID: 10025093936
• [Journal Article] Functional cyclodextrins polyrotaxanes for drug delivery (2009)
  • Author(s): 由井伸彦, R.Katoono, A.Yamashita
  • Journal Title: Advances in Polymer Science 222 Pages: 55-77
  • Peer Reviewed
• [Journal Article] Supramolecular mobility in polyrotaxanes exploits biomedical functions (2009)
  • Author(s): 由井伸彦
  • Journal Title: Macromolecular Symposia 279 Pages: 158-162
  • Peer Reviewed
• [Journal Article] Polyrotaxane composed of poly-L-lactide and alpha-cyclodextrin exhibiting protease triggering hydrolysis (2009)
  • Author(s): Y.Ohya, S.Takamido, K.Nagahama, T.Ouchi, R.Katoono, 由井伸彦
  • Journal Title: Biomacromolecules 10 Pages: 2261-2267
  • Peer Reviewed
• [Journal Article] Loose-Fit Polyrotaxane Composed of γ-CD and Single PEG Chain : Making Room in γ-CD Cavity for Additional Inclusion Complexation (2009)
  • Author(s): A.Takahashi, R.Katoono, 由井伸彦
  • Journal Title: Macromolecules 42 Pages: 8587-8589
  • Peer Reviewed
• [Journal Article] (2009)
  • Author(s): 由井伸彦(分担執筆)
  • Journal Title: 超分子サイエンス&テクノロジー (国武豊喜・監修)(エスティーエス)
• [Journal Article] Preparation of hydrolyzable polyrotaxane containing ester linkages and its degradati on behavior (2008)
  • Author(s): R. Katoono, S. Fukuda, H. J. Shin, N. Yui
  • Journal Title: Chemistry Letters 37(9) Pages: 988-989
  • Peer Reviewed
• [Journal Article] 超分子科学が拓く明日のバイオマテリアル (2008)
  • Author(s): 由井 伸彦
  • Journal Title: 化学工業 59(1) Pages: 10-15
  • Peer Reviewed
• [Journal Article] Bundling Two Polymeric Chains with gamma-Cyclodextrin Cavity Contributing to Supramolecular Network Formation (2007)
  • Author(s): R.Kawabata, R.Katoono, M.Yamaguchi, N.Yui
  • Journal Title: Macromolecules 40(4) Pages: 1011-1017
  • Peer Reviewed
• [Presentation] Kinetic analysis for multivalent interaction between mannose-conjugated polyrotaxanes and Con A-immobilized surface through SPR study (2011)
  • Author(s): H.Hyun, N.Yui
  • Organizer: International Conference on Biomaterials Science 2011
  • Place of Presentation: つくば
  • Year and Date: 2011-03-17
• [Presentation] Kinetic analysis for multivalent interaction.between mannose-conjugate polyrotaxanes and Con A-immobilized surface through SPR study (2011)
  • Author(s): Hoon Hyun, Nobuhiko Yui
  • Organizer: International Conference on Biomaterials Science2011
  • Place of Presentation: つくば
  • Year and Date: 2011-03-17
• [Presentation] Supramolecular polyrotaxanes as dynamic biomaterials (2010)
  • Author(s): 由井伸彦
  • Organizer: The 9th France-Japan DDS Symposium
  • Place of Presentation: 熊本
  • Year and Date: 2010-09-27
• [Presentation] Supramolecular polyrotaxanes as dynamic biomaterials (2010)
  • Author(s): Nobuhiko Yui
  • Organizer: The gth France-Japan DDS Symposium
  • Place of Presentation: 熊本
  • Year and Date: 2010-09-27
• [Presentation] スリッピングを利用したダンベル型PEGとp-cDダイマー/テトラマーによる可逆的超分子クロスリンク形成 (2010)
  • Author(s): 小林裕一郎、上遠野亮、山口政之、由井伸彦
  • Organizer: 第59回高分子討論会
  • Place of Presentation: 札幌
  • Year and Date: 2010-09-16
• [Presentation] γ-CDとPEGからなるルーズフィットポリロタキサンのゲスト分子包接 (2010)
  • Author(s): 高橋明裕、山田鋼、上遠野亮、由井伸彦
  • Organizer: 第59回高分子討論会
  • Place of Presentation: 札幌
  • Year and Date: 2010-09-16
• [Presentation] マンノース導入ポリロタキサンとCon A固定化表面との間の多価相互作用における速度論的解析 (2010)
  • Author(s): H.Hyun、上遠野亮、三浦佳子、由井伸彦
  • Organizer: 第27回シクロデキストリンシンポジウム
  • Place of Presentation: 金沢
  • Year and Date: 2010-09-06
• [Presentation] 加熱によって誘起されるダンベル型PEGとβ-CDダイマー/テトラマー間の超分子クロスリンク形成 (2010)
  • Author(s): 小林裕一郎、上遠野亮、山口政之、由井伸彦
  • Organizer: 第27回シクロデキストリンシンポジウム
  • Place of Presentation: 金沢
  • Year and Date: 2010-09-06
• [Presentation] γ-CDとPEGからなる水溶性ルーズフィットポリロタキサンのゲスト分子包接能 (2010)
  • Author(s): 高橋明裕、山田鋼、上遠野亮、由井伸彦
  • Organizer: 第27回シクロデキストリンシンポジウム
  • Place of Presentation: 金沢
  • Year and Date: 2010-09-06
• [Presentation] マンノース導入ポリロタキサンとCon-A固定化表面との間の多価相互作用における速度論的解析 (2010)
  • Author(s): H.Hyun、上遠野亮、三浦佳子、由井伸彦
  • Organizer: 第27回シクロデキストリンシンポジウム
  • Place of Presentation: 金沢
  • Year and Date: 2010-09-06
• [Presentation] ポリロタキサンの構造的特徴に基づいたバイオマテリアルの設計 (2010)
  • Author(s): 由井伸彦
  • Organizer: 第22回万有札幌シンポジウム
  • Place of Presentation: 札幌
  • Year and Date: 2010-07-03
• [Presentation] Supramolecular surfaces modulating cellular response (2010)
  • Author(s): Nobuhiko Yui
  • Organizer: The 5^<th> Forum on New Materials
  • Place of Presentation: イタリア
  • Year and Date: 2010-06-14
• [Presentation] Polyrotaxane surfaces as dynamic nanobio-interfaces (2010)
  • Author(s): Nobuhiko Yui
  • Organizer: The 5^<th> International Symposium on Macrocyclic&Supramolecular Chemistry
  • Place of Presentation: 奈良
  • Year and Date: 2010-06-09
• [Presentation] 末端にベンズアミド基を有するPEGとR-CDダイマー/テトラマーから形成される超分子クロスリンク (2010)
  • Author(s): 小林裕一郎、上遠野亮、由井伸彦
  • Organizer: 第59回高分子学会年次大会
  • Place of Presentation: 横浜
  • Year and Date: 2010-05-28
• [Presentation] Exploiting polyrotaxane structures as advanced biomaterials (2010)
  • Author(s): 由井伸彦
  • Organizer: JAIST-CNSI Workshop
  • Place of Presentation: 北陸先端科学技術大学院大学
  • Year and Date: 2010-01-18
• [Presentation] バイオマテリアルとしての動的表面設計 (2009)
  • Author(s): 由井伸彦
  • Organizer: 第10回リング・チューブ超分子研究会
  • Place of Presentation: 東京大学柏キャンパス
  • Year and Date: 2009-12-11
• [Presentation] PEGとγ-CDからなる1分子鎖"すかすか"ポリロタキサン (2009)
  • Author(s): 高橋明裕、上遠野亮、由井伸彦
  • Organizer: 第26回シクロデキストリンシンポジウム
  • Place of Presentation: 宇都宮市民ホール
  • Year and Date: 2009-09-09
• [Presentation] PEGと・-CDからなる1分子鎖 "すかすか" ポリロタキサン (2009)
  • Author(s): 高橋明裕, 上遠野亮, 由井伸彦
  • Organizer: 第26回シクロデキストリンシンポジウム
  • Place of Presentation: 宇都宮市民ホール
  • Year and Date: 2009-09-09
• [Presentation] PEGとγ-CDからなる1分子鎖 "すかすか" ポリロタキサン (2009)
  • Author(s): 高橋明裕, 上遠野亮, 由井伸彦
  • Organizer: 第26回シクロデキストリンシンポジウム
  • Place of Presentation: 宇都宮市民ホール
  • Year and Date: 2009-09-09
• [Presentation] ポリ乳酸を主軸とする生分解性ポリロタキサンのプロテアーゼをトリガーとした超分子解離による分解挙動 (2009)
  • Author(s): 大矢裕一、長濱宏治、高御堂成剛、坂上浩美、大内辰郎、由井伸彦
  • Organizer: 第38回医用高分子シンポジウム
  • Place of Presentation: 東京大学先端科学技術研究センター
  • Year and Date: 2009-07-27
• [Presentation] 糖鎖導入ポリロタキサンの多価相互作用への寄与 (2009)
  • Author(s): 齋藤祐介、山口順、上遠野亮、三浦佳子、由井伸彦
  • Organizer: 第38回医用高分子シンポジウム
  • Place of Presentation: 東京大学先端科学技術研究センター
  • Year and Date: 2009-07-27
• [Presentation] 超分子構造に基づくマンノース導入ポリロタキサンの多価相互作用への寄与 (2008)
  • Author(s): 齊藤祐介, 上遠野亮, 三浦佳子, 由井伸彦
  • Organizer: 日本バイオマテリアル学会シンポジウム2008
  • Place of Presentation: 東京
  • Year and Date: 2008-11-17
• [Presentation] Supramolecular Mobility in Polyrotaxanes Exploits Biomedical Functions (2008)
  • Author(s): N. Yui
  • Organizer: IUPAC MACR02008
  • Place of Presentation: 台北、台湾
  • Year and Date: 2008-07-01
• [Presentation] Preparation of hydrogels based on hydrolyzable polyrotaxane (2008)
  • Author(s): H. J. Shin, S. Fukuda, R. Katoono, N. Yui
  • Organizer: IUPACMACR02008
  • Place of Presentation: 台北、台湾
  • Year and Date: 2008-06-30
• [Presentation] 末端に構造的異方性基を有する軸分子を用いたポリロタキサンの形成挙動 (2008)
  • Author(s): 小林裕一郎, 上遠野亮, 由井伸彦
  • Organizer: 第57回高分子学会年次大会
  • Place of Presentation: 横浜
  • Year and Date: 2008-05-29
• [Presentation] 加水分解性ポリロタキサンヒドロゲルの合成 (2008)
  • Author(s): 辛昊俊, 上遠野亮, 由井伸彦
  • Organizer: 第57回高分子学会年次大会
  • Place of Presentation: 横浜
  • Year and Date: 2008-05-28
• [Presentation] CDとCBからなるポリロタキサンの合成とその電気特性(3Pc155) (2007)
  • Author(s): 小林 裕一郎・上遠 野亮・由井 伸彦・KIM, Kimoon
  • Organizer: 第56回高分子学会年次大会
  • Place of Presentation: 国立京都国際会館(京都市)
  • Year and Date: 2007-05-29
• [Presentation] 二分子の高分子鎖を環状分子で束ねた構造を持つ高分子包接錯体のネットワーク形成への寄与(3Pc165) (2007)
  • Author(s): 河端 良二・上遠 野亮・山口 政之・由井 伸彦
  • Organizer: 第56回高分子学会年次大会
  • Place of Presentation: 国立京都国際会館(京都市)
  • Year and Date: 2007-05-29
• [Book] エスティーエス,超分子サイエンス&テクノロジー(国武豊喜・監修) (2009)
  • Author(s): 由井伸彦(分担執筆)
  • Total Pages: 1340
• [Book] エスティーエス, 超分子サイエンス&テクノロジー(国武豊喜・監修) (2009)
  • Author(s): 由井伸彦(分担執筆)
  • Total Pages: 1340
• [Remarks] ホームページ等