Management of proarrhythmias according to the cardiac stem cell therapy

• Project/Area Number: 19300175
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biomedical engineering/Biological material science
• Research Institution: Keio University
• Principal Investigator: MIYOSHI Syunichiro Keio University, 医学部, 講師 (10296577)
• Co-Investigator(Kenkyū-buntansha): HIDA Naoko 慶應義塾大学, 医学部, 助教 (70360112)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000); Fiscal Year 2008: ¥8,970,000 (Direct Cost: ¥6,900,000、Indirect Cost: ¥2,070,000); Fiscal Year 2007: ¥9,880,000 (Direct Cost: ¥7,600,000、Indirect Cost: ¥2,280,000)
• Keywords: Cell sheet / stem cell / human / gene / arrhythmia / 心筋誘導 / 間葉系幹細胞 / 細胞移植 / Arrhythmia

Research Abstract

心臓分野での再生医療の実現に向けて、ヒト間葉系幹細胞を用いた研究を行った。ヒト間葉系幹細胞の心筋分化能力を改善させ、それにより移植時の治療効果改善効果目指す研究を行った。また同時に、心臓再生医療で重大な問題となる催不整脈性についての検証を行い、新筋再生の能力が高まれば電気的に安定し、催不整脈性の心配は減じる可能性が考えられた。

Research Products

• [Journal Article] Images in cardiovascular medicine. Pseudonormal position of an atrial pacemaker lead associated with a contralateral pneumothorax : where is the atrial lead? (2008)
  • Author(s): S Miyoshi
  • Journal Title: Circulation 117(17) Pages: 2297-2298
  • Peer Reviewed
• [Journal Article] Novel cardiac precursor-like cells from human menstrual blood-derived mesenchymal cells (2008)
  • Author(s): N. Hida, S Miyoshi
  • Journal Title: Stem Cells 26(7) Pages: 1695-1704
  • Peer Reviewed
• [Journal Article] 骨髄以外のヒト間葉系細胞からの心筋分化 (2008)
  • Author(s): 三好俊一郎
  • Journal Title: 心電図 28(3) Pages: 37-48
  • NAID: 10025583776
  • Peer Reviewed
• [Journal Article] 心筋再生と不整脈心筋細胞シート移植片のホストとの電気的同期現象 (2008)
  • Author(s): 三好俊一郎, 肥田直子
  • Journal Title: 心電図 28(4)(招待) Pages: 309-309
  • Peer Reviewed
• [Journal Article] Pseudonormal position of an atrial pacemaker lead associated With a contralateral pneumothorax : where is the atrial lead? (2008)
  • Author(s): Yada H, Miyoshi S
  • Journal Title: Circulation 117・17 Pages: 2297-2298
  • Peer Reviewed
• [Journal Article] Novel cardiac precursor -like cells from human menstrual blood -derived mesenchymal cells. (2008)
  • Author(s): Hida N, Miyoshi S
  • Journal Title: Stem Cells 26・7 Pages: 1695-1704
  • Peer Reviewed
• [Journal Article] 循環器学2008年の進歩 不整脈研究の進歩 (2008)
  • Author(s): 小川聡, 三好俊一郎
  • Journal Title: 循環器専門医 16・2 Pages: 332-338
• [Journal Article] 【臨床遺伝子学'08】不整脈と遺伝子 (2008)
  • Author(s): 小川聡, 三好俊一郎
  • Journal Title: 最新医学 63・9月増刊 Pages: 1916-1923
• [Journal Article] 心筋細胞シート移植片とホスト心筋との電気的同期現象 (2008)
  • Author(s): 三好俊一郎
  • Journal Title: 心電図 28・4 Pages: 309-315
  • NAID: 10024857191
  • Peer Reviewed
• [Journal Article] 骨髄以外のヒト開葉系細胞からの心筋分化 (2008)
  • Author(s): 三好俊一郎
  • Journal Title: 心電図 28・suppl3 Pages: 37-45
  • Peer Reviewed
• [Journal Article] 左室からの通電を要したPosterior Coronary Veinに付着した副伝導路を介する房室回帰性頻拍 (2008)
  • Author(s): 谷本耕司郎, 三好俊一郎
  • Journal Title: 臨床心臓電気生理 31 Pages: 9-16
  • Peer Reviewed
• [Journal Article] SHP2-mediated signaling cascade through gp130 is essential for LIF-dependent I CaL, [Ca2+]i transient, and APD increase in cardiomyocytes (2007)
  • Author(s): S Miyoshi
  • Journal Title: J Mol Cell Cardiol 43(6) Pages: 710-6
  • Peer Reviewed
• [Journal Article] 'Working' cardiomyocytes exhibiting plateau action potentials from human placenta-derived extraembryonic mesodermal cells (2007)
  • Author(s): S Miyoshi, N. Hida
  • Journal Title: Exp Cell Res. 313(12) Pages: 2550-2562
  • Peer Reviewed
• [Journal Article] The significant cardiomyogenic potential of human umbilical cord blood-derived mesenchymal stem cells in vitro (2007)
  • Author(s): S Miyoshi, N. Hida
  • Journal Title: Stem Cells. 25(8) Pages: 2017-2024
  • Peer Reviewed
• [Journal Article] Sema3a maintains normal heart rhythm through sympathetic innervation patterning (2007)
  • Author(s): S Miyoshi
  • Journal Title: Nat Med. 13(5) Pages: 604-612
  • Peer Reviewed
• [Journal Article] Cardiac cell therapy and arrhythmias (2007)
  • Author(s): S Miyoshi
  • Journal Title: Circ J 71
  • NAID: 110006318432
  • Peer Reviewed
• [Journal Article] Application of mesenchymal stem cell-derived cardiomyocytes as bio-pacemakers : current status and problems to be solved (2007)
  • Author(s): S Miyoshi
  • Journal Title: Med Biol Eng Comput 45(2) Pages: 209-220
  • Peer Reviewed
• [Journal Article] 心筋再生と不整脈再生医療の将来と不整脈 (2007)
  • Author(s): 三好俊一郎
  • Journal Title: 心臓 39(11)(招待) Pages: 1017-1022
  • Peer Reviewed
• [Journal Article] Tissue engineering analyses of cell viability and metabolism in novel 3-dimensional tissues composed of multi-layered cell sheets (2007)
  • Author(s): S Miyoshi
  • Journal Title: 人工臓器 36(2) Pages: 14-14
  • Peer Reviewed
• [Journal Article] 心筋再生と不整脈再生医療の将来と不整脈 (2007)
  • Author(s): 三好 俊一郎(1/15)
  • Journal Title: 心臓 39 Pages: 1017-1022
  • Peer Reviewed
• [Journal Article] 'Working' cardiomyocytes exhibiting plateau action potentials from human placenta-derived extraembryonic mesodermal cells. (2007)
  • Author(s): Okamoto K, Mioshi S(2/21)
  • Journal Title: Exp Cell Res. 313 Pages: 2550-2562
  • Peer Reviewed
• [Journal Article] The significant cardiomyogenic potential of human umbilical cord blood-derived mesenchymal stem cells in vitro. (2007)
  • Author(s): Nishiyama N, Miyoshi S(2/12)
  • Journal Title: Stem Cells 25 Pages: 2017-2024
  • Peer Reviewed
• [Presentation] Pretreatment of Human Marrow-Derived Mesenchymal Stem Cells With Pioglitazone Improved the Efficacy of Transplantation on Cardiac Function In Vivo (2009)
  • Author(s): Shinmura D, Miyoshi S
  • Organizer: THE AMERICAN COLLEGE OF CARDIOLOGY
  • Place of Presentation: オーランド、アメリカ
  • Year and Date: 2009-03-30
• [Presentation] Xenografted human amniotic membrane-derived mesenchyma1 stem cell acquired immunological tolerance and transdifferentiated to cardiomyocyte in vivo. (2008)
  • Author(s): Tsuji H, Miyoshi S
  • Organizer: American Heart Association
  • Place of Presentation: ニューオーリンズ、アメリカ
  • Year and Date: 2008-11-11
• [Presentation] Xenografted human amniotic membrane-derived mesenchymal stem cells acquired immunologic tolerance and transdifferentiated into cardiomyocytes in vivo (2008)
  • Author(s): S Miyoshi
  • Organizer: ESHRE 2008(poster award 賞受賞)
  • Place of Presentation: バルセロナ
  • Year and Date: 2008-07-09
• [Presentation] Human amniotic membrane-derived mesenchymal stem cell acquired immunological tolerance by HLA-G expression and differentiated cardiomyocyte in vivo (2008)
  • Author(s): Tsuji H, Miyoshi S
  • Organizer: European Society of Human Reproduction & Embryology
  • Place of Presentation: バルセロナ、スペイン
  • Year and Date: 2008-07-09
• [Presentation] Human amniotic membrane-derived mesenchymal stem cell acquired immune tolerance by HLA-G expression and differentiated into cardiomyocyte in vivo (2008)
  • Author(s): S Miyoshi
  • Organizer: JACC(51(10) ; A182, 2008)
  • Place of Presentation: シカゴ
  • Year and Date: 2008-03-30
• [Presentation] Non marrow-derived human mesenchymal cells are promising cellular source for cardiac stem cell therapy (2007)
  • Author(s): S Miyoshi
  • Organizer: Joint Cardiology Symposium 2007(韓国Gwangju-Boston)
  • Place of Presentation: South Korea(招待講演)
  • Year and Date: 2007-11-17
• [Presentation] 心筋再生と不整脈 (2007)
  • Author(s): 三好俊一郎
  • Organizer: 頻拍症カンファレンス2007、シンポジスト(再生医療の将来と不整脈)
  • Place of Presentation: 名古屋
  • Year and Date: 2007-10-05
• [Presentation] 心筋再生と不整脈心筋細胞シート移植片のホストとの電気的同期現象 (2007)
  • Author(s): 三好 俊一郎(1/1)
  • Organizer: 第24回日本心電図学会学術集会
  • Place of Presentation: 名古屋
  • Year and Date: 2007-10-05
• [Presentation] ヒト間葉系幹細胞由来心、筋細胞の電気生理学的特性(招待講演) (2007)
  • Author(s): 三好俊一郎
  • Organizer: 第5回昭和再生医学医療研究会
  • Year and Date: 2007-09-28
• [Presentation] 骨髄医学のヒト間葉系細胞からの心筋分化(招待講演) (2007)
  • Author(s): 三好俊一郎
  • Organizer: 第22回犬山不整脈カンファレンス
  • Year and Date: 2007-08-25
• [Presentation] Human menstrual blood-derived mesenchymal stem cell can restore impaired cardiac function by cardiomyogenesis in vivo (2007)
  • Author(s): S Miyoshi
  • Organizer: JACC(49(9) ; 83A, 2007)
  • Place of Presentation: ニューオリンズ
  • Year and Date: 2007-03-26
• [Presentation] Human amniotic membrane-derived stem cell with lack of HLA-DR expression can be a promising cellular source for cardiac stem cell therapy (2007)
  • Author(s): S Miyoshi
  • Organizer: JACC(49(9) ; 47A, 2007)
  • Place of Presentation: ニューオリンズ
  • Year and Date: 2007-03-25
• [Presentation] In situ cardiomyogenic transdifferentiation potential of human uterine endometrium-derived mesenchymal cell (2007)
  • Author(s): S Miyoshi
  • Organizer: JACC(49(9) ; 42A, 2007)
  • Place of Presentation: ニューオリンズ
  • Year and Date: 2007-03-25
• [Presentation] Human mesenchymal stem cell from human chorionic villi is a new cell source for autologous cardiac stem cell therapy (2007)
  • Author(s): S Miyoshi
  • Organizer: JACC(49(9) ; 53A, 2007)
  • Place of Presentation: ニューオリンズ
  • Year and Date: 2007-03-25
• [Presentation] In Situ Cardiomyogenic Transdifferentiation Potential of Human Uterine Endometrium-derived Mesenchymal Cell (2007)
  • Author(s): S Miyoshi
  • Organizer: Circ J(71 ; 177, 2007)
  • Place of Presentation: 神戸
  • Year and Date: 2007-03-17
• [Presentation] Human Mesenchymal Stem Cell from Human Chorionic Villi is a New Cell Source for Autologous Cardiac Stem Cell Therapy (2007)
  • Author(s): S Miyoshi
  • Organizer: Circ J(71 ; 464, 2007)
  • Place of Presentation: 神戸
  • Year and Date: 2007-03-17
• [Presentation] Serum-independent Cardiomyogenic Differentiation in Human Endometrial-derived and Menstrual Blood-derived Mesencymal Stem Cells for Drug Screening System (2007)
  • Author(s): S Miyoshi
  • Organizer: Circ J(71 ; 466, 2007)
  • Place of Presentation: 神戸
  • Year and Date: 2007-03-17
• [Presentation] Human Amniotic Membrane-derived Stem Cell with no HLA-DR Expression can be a Promising Cellular Source for Cardiac Stem Cell Therapy (2007)
  • Author(s): S Miyoshi
  • Organizer: Circ J(71 ; 177, 2007)
  • Place of Presentation: 神戸
  • Year and Date: 2007-03-15
• [Book] 【再生医学とリプロダクション】胎盤由来の間葉系幹細胞の心筋分化産(59(3))(招待) (2007)
  • Author(s): 三好俊一郎
  • Publisher: 婦人科の世界
• [Remarks] 「月経血心筋の母」朝日新聞朝刊34頁, 2008/4/18(査読無し)
• [Remarks] 「月経血から心筋細胞」読売新聞朝刊33頁、2008/4/20(査読無し)
• [Remarks] Scientists say menstrual blood can repair hearts, Yahoo News AFP 通信2008/4/24(査読無し)
• [Remarks]
  • URL: http://cardioweb.cpnet.med.keio.ac.jp/Recr/RES/Physiol/index.html
• [Patent(Industrial Property Rights)] 心筋細胞分化誘導促進剤及びその使用方法 (2007)
  • Inventor(s): 三好俊一郎
  • Industrial Property Rights Holder: 三好俊一郎、小川聡、梅澤明弘
  • Industrial Property Number: 2007-315920
  • Filing Date: 2007-12-06
• [Patent(Industrial Property Rights)] 心筋細胞分化誘導促進剤及びその使用方法 (2007)
  • Inventor(s): 三好俊一郎
  • Industrial Property Rights Holder: 三好俊一郎、小川聡、梅澤明弘
  • Industrial Property Number: 2007-315921
  • Filing Date: 2007-12-06
• [Patent(Industrial Property Rights)] 細胞噴射装置 (2007)
  • Inventor(s): 三好俊一郎
  • Industrial Property Rights Holder: 三好俊一郎、小川聡、梅澤明弘, オリンパス株式会社
  • Acquisition Date: 2007-11-01