Application of Near-Infrared Fluorescence for Medical Photonics

• Project/Area Number: 19300178
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biomedical engineering/Biological material science
• Research Institution: Tokyo University of Science
• Principal Investigator: 曽我 公平 Tokyo University of Science, 基礎工学部・材料工学科, 准教授 (50272399)
• Co-Investigator(Kenkyū-buntansha): NAGASAKI Yukio 筑波大学, 数理物質科学研究所, 教授 (90198309)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥19,110,000 (Direct Cost: ¥14,700,000、Indirect Cost: ¥4,410,000); Fiscal Year 2008: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000); Fiscal Year 2007: ¥11,960,000 (Direct Cost: ¥9,200,000、Indirect Cost: ¥2,760,000)
• Keywords: アップコンバージョン / 近赤外 / フォトダイナミックセラピー / 蛍光イムノアッセイ / 希土類 / イムノアッセイ / 発光体

Research Abstract

生体へのダメージが少なく、散乱が弱いために生体深部へ到達可能な近赤外光を励起光とした蛍光イムノアッセイ(LIA)とフォトダイナミックセラピー(PDT)のための材料開発を目指し、LIAのためのUC発光粒子の合成と表面修飾による粒子の分散安定性の付与、PEG-b-PAAcとストレプトアビジンの共固定による特異的吸着性の付与、PDTのためのUC発光粒子表面のシランカップリング剤による修飾とポルフィリン色素の導入およびチタン含有相の形成に成功した

Research Products

• [Journal Article] 希土類含有セラミックスナノ粒子によるアップコンバージョン現象を利用した近赤外励起バイオイメージング (2008)
  • Author(s): 曽我公平・上村真生・長崎幸夫
  • Journal Title: 応用物理 77 Pages: 1458-1461
  • NAID: 10024401895
• [Journal Article] Preparation of PEGylated upconversion nanophosphors with highdispersion stability under physiologicalconditions for near-infrared bioimaging (2008)
  • Author(s): M. Kammimura, D. Miyamoto, Y. Saito, K. Soga, Y. Nagasaki
  • Journal Title: Transactions of the Materials Research Society of Japan 33 Pages: 795-798
• [Journal Article] Plate assay by UC emission from Y2O3 : Er under nearinfrared excitation (2008)
  • Author(s): Y. Saito, K. Shimizu, M. Kamimura, H. Furusho, K. Soga and Y. Nagasaki
  • Journal Title: Transactions of the Materials Research Society of Japan 33 Pages: 803-806
• [Journal Article] Improvement of dispersion stability and characterization of upconversion nanophosphorscovalently modified with PEG as a fluorescencebioimaging probe (2008)
  • Author(s): Tamotsu Zako, Hiroyasu Nagata, Naofumi Terada, Masafumi Sakono, Kohei Soga, MizuoMaeda
  • Journal Title: JOURNAL OF MATERIALS SCIENCE 45 Pages: 5325-5330
• [Journal Article] Preparation of PEG and Protein Co-immobilized Upconversion Nanophosphors as Near-infraredBiolabeling Materials (2008)
  • Author(s): Masao Kamimura, Daisuke Miyamoto, Yu Saito, Kohei Soga and Yukio Nagasaki
  • Journal Title: JOURNAL OF PHOTOPOLYMER SCIENCE ANDTECHNOLOGY 21 Pages: 183-187
• [Journal Article] Design of Poly(ethylene glycol)/StreptavidinCoimmobilized Upconversion Nanophosphorsand Their Application to FluorescenceBiolabeling (2008)
  • Author(s): Masao Kamimura, Daisuke Miyamoto, Yu Saito, Kohei Soga and Yukio Nagasaki
  • Journal Title: LANGMUIR 24 Pages: 8864-8870
• [Journal Article] 希土類含有セラミックスナノ粒子によるアップコンバージョン現象を利用した近赤外励起バイオイメージング (2008)
  • Author(s): 曽我公平, 上村真生, 長崎幸夫
  • Journal Title: 応用物理 77 Pages: 1458-1461
  • NAID: 10024401895
  • Peer Reviewed
• [Journal Article] Preparation of PEGylated upconversion nanophosphors with high dispersion stability under physiological conditions for near-infrared bioimaging (2008)
  • Author(s): M. Kammimura, D. Miyamoto, Y. Saito, K. Soga, Y. Nagasaki
  • Journal Title: Transactions of the Materials Research Society of Japan 33 Pages: 795-798
  • Peer Reviewed
• [Journal Article] Plate assay by UC emission from Y_2O_3 : Er under near infrared excitation (2008)
  • Author(s): Y. Saito, K. Shimizu, M. Kamimura, H. Furusho, K. Soga, Y. Nagasaki
  • Journal Title: Transactions of the Materials Research Society of Japan 33 Pages: 803-806
  • Peer Reviewed
• [Journal Article] Improvement of dispersion stability and characterization of upconversion nanophosphors covalently modified with PEG as a fluorescence bioimaging probe (2008)
  • Author(s): Tamotsu Zako, Hiroyasu Nagata, Naofumi Terada, Masafumi Sakono, Kohei Soga, Mizuo Maeda
  • Journal Title: JOURNAL OF MATERIALS SCIENCE 45 Pages: 5325-5330
  • Peer Reviewed
• [Journal Article] Preparation of PEG and Protein Co-immobilized Upconversion Nanophosphors as Near-infrared Biolabeling Materials (2008)
  • Author(s): Masao Kamimura, Daisuke Miyamoto, Yu Saito, Kohei Soga, Yukio Nagasaki
  • Journal Title: JOURNAL OF PHOTOPOLYMER SCIENCE AND TECHNOLOGY 33 Pages: 803-806
  • NAID: 130004464618
  • Peer Reviewed
• [Journal Article] Application of Rare-Earth Doped Ceramic Nanophoshpors for Bioimaging under NIR Excitation (2007)
  • Author(s): Kohei Soga, Yu Saito, Kazuaki Shimizu, Yoshihito Okumura and Tomoya Konishi
  • Journal Title: Proceedings of Materials Science and Technology 2007 Pages: 617-621
• [Journal Article] Plate assay by UC emission from Y203: Er3+ under near infrared excitation
  • Author(s): Y. Saito, K. Shimizu, M. Kamimura^*, H. Furusyo, K. Soga and Y. Nagasaki
  • Journal Title: Transactions of Materials Society Japan(Accepted)
  • Peer Reviewed
• [Presentation] 希土類含有セラミックスナノ粒子を用いた近赤外励起バイオイメージング電気化学会 (2009)
  • Author(s): 曽我公平
  • Organizer: 【招待講演】第76回大会
  • Place of Presentation: 京都府京都市・京都大学
• [Presentation] Use of Phosphate Salt as a Sintering Inhibitor onSynthesis of Y_2O_3 Nanoparticles forBioimaging, 3rd International Symposium on Atomic Technology (ISAT-3)/3rdPolyscale Technology Workshop (PTW-3) (2009)
  • Author(s): Yoshihito OKUMURA, Kosuke TSUJI, NallusamyVENKATACHALAM, Kohei SOGA
  • Organizer: 【ポスター】
  • Place of Presentation: 東京都江東区・国際交流館
• [Presentation] バイオイメージング用酸化イットリウムナノ粒子作製におけるリン酸塩の添加効果 (2009)
  • Author(s): 奥村承士, 齋藤悠, 清水和明,小西智也, 曽我公平
  • Organizer: 【一般講演】第47 回セラミックス基礎科学討論会
  • Place of Presentation: 大阪府大阪市・大阪国際会議場
• [Presentation] 希土類含有セラミックスナノ粒子を用いた近赤外励起バイオイメージング (2009)
  • Author(s): 曽我公平
  • Organizer: 電気化学会第76回大会
  • Place of Presentation: 京都府京都市・京都大学
• [Presentation] Use of Phosphate Salt as a Sintering Inhibitor on Synthesis of Y_2O_3 Nanoparticles for Bioimaging (2009)
  • Author(s): Yoshihito OKUMURA, Kosuke TSUJI, Nallusamy VENKATAC HALAM, Kohei SOGA
  • Organizer: 3rd International Symposium on Atomic Technology (ISAT-3)/3rd Polyscale Technology Workshop (PTW-3)
  • Place of Presentation: 東京都江東区・国際交流館
• [Presentation] バイオイメージング用酸化イットリウムナノ粒子作製におけるリン酸塩の添加効果 (2009)
  • Author(s): 奥村承士, 齋藤悠, 清水和明, 小西智也, 曽我公平
  • Organizer: 第47回セラミックス基礎科学討論会
  • Place of Presentation: 大阪府大阪市・大阪国際会議場
• [Presentation] Application of Rare-Earth Doped CeramicNanophosphors for NIR Biophotonics (2008)
  • Author(s): Y. Okumura, K. Tokuzen, N. Venkatachalam and K. Soga
  • Organizer: 【ポスター】Kashiwa Symposium on Cancer Biology 2008
  • Place of Presentation: Kashiwa, Chiba, Japan
  • Year and Date: 2008-11-14
• [Presentation] Development of Nanophoshpors for NIR Fluorescence Bioimaging (2008)
  • Author(s): Kohei Soga, Kimikazu Tokuzen, Yoshihito Okumura, MasaoKamimura and Yukio Nagasaki
  • Organizer: 【一般講演】2008 MRS Fall Meeting
  • Place of Presentation: Boston, MA, USA
• [Presentation] 近赤外励起蛍光バイオイメージング用発光マーカーの開発-生体にやさしいバイオイメージングを目指して- (2008)
  • Author(s): 曽我公平
  • Organizer: 【招待講演】第57 回高分子討論会
  • Place of Presentation: Osaka, Osaka
• [Presentation] 希土類含有セラミックスナノ粒子による近赤外励起バイオイメージング (2008)
  • Author(s): 曽我公平, 奥村承士, 斎藤悠,上村真生, 長崎幸夫
  • Organizer: 【一般講演】日本セラミックス協会第21回秋季シンポジウム
  • Place of Presentation: Kitakyushu, Fukuoka, Japan
• [Presentation] NIR Biophotonics by Rare-Earth DopedNanophosphors Composited withBiofunctional Polymer (2008)
  • Author(s): Kohei SOGA
  • Organizer: 【招待講演】Materials Science and Engineering 2008 (MSE 08)
  • Place of Presentation: Nurnberg, Germany
• [Presentation] Application of Rare-Earth Doped Yttrium Oxide forFluorescence Bioimaging under NIRexcitation (2008)
  • Author(s): Yoshihito OKUMURA, Yu SAITO, Kazuaki SHIMIZU, RyunosukeKUNINOBU, Masao KAMIMURA, KoheiSOGA and Yukio NAGASAKI
  • Organizer: 【一般講演】The 15th International Conference on Luminescence and OpticalSpectroscopy of Condensed Matter (ICL'08)
  • Place of Presentation: Lyon, France
• [Presentation] Ceramic Nanophosphors for UpconversionBioimaging (2008)
  • Author(s): K. Soga, Y. Saito, M. Kamimura and Y. Nagasaki
  • Organizer: 【ポスター】Nanotech2008
  • Place of Presentation: Boston, MA, USA
• [Presentation] Development of Nanophoshpors for NIR Fluorescence Bioimaging (2008)
  • Author(s): Kohei Soga, Kimikazu Tokuzen, Yoshihito Okumura, Masao Kamimura, Yukio Nagasaki
  • Organizer: 2008 MRS Fall Meeting
  • Place of Presentation: Boston, MA, USA
• [Presentation] Application of Rare-Earth Doped Ceramic Nanophosphors for NIR Biophotonics (2008)
  • Author(s): Y. Okumura, K. Tokuzen, N. Venkatachalam, K. Soga
  • Organizer: Kashiwa Symposium on Cancer Biology 2008
  • Place of Presentation: Kashiwa, Chiba, Japan
• [Presentation] 近赤外励起蛍光バイオイメージング用発光マーカーの開発-生体にやさしいバイオイメージングを目指して- (2008)
  • Author(s): 曽我公平
  • Organizer: 第57回高分子討論会
  • Place of Presentation: Osaka, Osaka
• [Presentation] 希土類含有セラミツクスナノ粒子による近赤外励起バイオイメージング (2008)
  • Author(s): 曽我公平, 奥村承士, 斎藤悠, 上村真生, 長崎幸夫
  • Organizer: 日本セラミックス協会第21回秋季シンポジウム
  • Place of Presentation: Kitakyushu, Fukuoka, Japan
• [Presentation] NIR Biophotonics by Rare-Earth Doped Nanophosphors Composited with Biofunctional Polymer (2008)
  • Author(s): Kohei SOGA
  • Organizer: Materials Science and Engineering 2008MSE 08
  • Place of Presentation: Nurnberg, Germany
• [Presentation] Application of Rare-Earth Doped Yttrium Oxide for Fluorescence Bioimaging under NIR excitation (2008)
  • Author(s): Yoshihito OKUMURA, Yu SAITO, Kazuaki SHIMIZU, Ryunosuke KUNINOBU, Masao KAMIMURA, Kohei SOGA, Yukio NAGASAKI
  • Organizer: The 15th International Conference on Luminescence and Optical Spectroscopy of Condensed Matter (ICL'08)
  • Place of Presentation: Lyon, France
• [Presentation] Ceramic Nanophosphors for Upconversion Bioimaging (2008)
  • Author(s): K. Soga, Y. Saito, M. Kamimura, Y. Nagasaki
  • Organizer: Nanotech2008
  • Place of Presentation: Boston, MA, USA
• [Presentation] 希土類含有セラミックナノ粒子を用いた近赤外励起バイオフォトニックインターフェースの開拓 (2007)
  • Author(s): 曽我公平、奥村承士、斎藤悠、清水和明、永田浩康、座古保、前田瑞夫、上村真生、古性均、長崎幸夫
  • Organizer: 【一般講演】第18回日本MRS 学術シンポジウム
  • Place of Presentation: 東京
• [Presentation] Y_2O_3 : Er^<3+>の近赤外励起アップコンバージョン発光を利用した新規プレートアッセイ (2007)
  • Author(s): 齋藤悠, 清水和明, 曽我公平, 上村真生, 古性均, 長崎幸夫
  • Organizer: 【ポスター】第18回日本MRS学術シンポジウム
  • Place of Presentation: 東京
• [Presentation] 蛍光バイオイメージングを目指した水中分散安定性の高いPEG化アップコンバージョン発光ナノ粒子の調製 (2007)
  • Author(s): 上村真生、宮本大輔、長崎幸夫、齋藤悠、曽我公平
  • Organizer: 【ポスター】第18回日本MRS学術シンポジウム
  • Place of Presentation: 東京
• [Presentation] 近赤外光の蛍光バイオイメージングへの応用 (2007)
  • Author(s): 曽我公平
  • Organizer: 【招待講演】第16回日本バイオイメージング学会学術集会
  • Place of Presentation: 千葉県野田市・東京理科大
• [Presentation] 近赤外光の蛍光バイオイメージングへの応用(招待講演) (2007)
  • Author(s): 曽我公平
  • Organizer: 第16回日本バイオイメージング学会学術集会
  • Place of Presentation: Noda, Chiba
• [Book] 量子ドットの生命科学領域への応用 第8章フォトニックナノ粒子の近赤外励起バイオフォトニクスへの応用 (2007)
  • Author(s): 曽我公平(分担執筆)
  • Total Pages: 11
  • Publisher: シーエムシー出版
• [Patent(Industrial Property Rights)] アップコンバージョン発光粒子の有機色素による表面機能化 (2007)
  • Inventor(s): 曽我公平
  • Industrial Property Rights Holder: 学校法人東京理科大学
  • Filing Date: 2007-07-21