Development of a novel tumor diagnostics based on the imaging of the optically coded cancer cells

• Project/Area Number: 19300189
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Medical systems
• Research Institution: National Institute of Advanced Industrial Science and Technology
• Principal Investigator: KAUL Sunil National Institute of Advanced Industrial Science and Technology, セルエンジニアリング研究部門, 主任研究員 (10356751)
• Co-Investigator(Kenkyū-buntansha): YOSHIZAKI Shinya 独立行政法人産業技術総合研究所, セルエンジニアリング研究部門, 研究員 (40415766) ; WADHWA Renu 独立行政法人産業技術総合研究所, セルエンジニアリング研究部門, グループ長 (30371090)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000); Fiscal Year 2009: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2008: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2007: ¥9,880,000 (Direct Cost: ¥7,600,000、Indirect Cost: ¥2,280,000)
• Keywords: 癌 / 細胞 / 抗体 / 内在化 / モータリン / 量子ドット / 診断 / デリバリー

Research Abstract

We have generated mortalin specific antibodies that showed cell-internalizing features. These antibodies were validated as nano-carriers. Antibodies were conjugated with quantum dots, FITC and expression plasmids that were delivered to cancer cells successfully. Cancer cells were labeled with various kinds of quantum dots to generate multicolor cells for long term imaging. We have demonstrated that (1) the antibody can be used for optic tagging of cells with quantum dots, (2) the optically tagged cells can be used for long term imaging of cells and (3) the tagged cells undergo normal differentiation in vitro and in vivo.

Research Products

• [Journal Article] The versatile stress protein mortalin as a chaperone therapeutic agent. (2009)
  • Author(s): Deocaris, C.C., Kaul, S.C., Wadhwa, R
  • Journal Title: Protein & Peptide Lett 16 Pages: 517-529
  • Peer Reviewed
• [Journal Article] Stable and nondisruptive in vitro/in vivo labeling of mesenchymal stem cells by internalizing quantum dots. (2009)
  • Author(s): Ohyabu, Y., Kaul, Z., Yoshioka, T., Inoue, K., Sakai, S., Mishima, H., Uemura, T., Kaul, S.C., Wadhwa, R
  • Journal Title: Human Gene Therapy 20 Pages: 217-224
  • Peer Reviewed
• [Journal Article] Stable and non-disruptive in vitrolin vivo labelling of mesenchymal stem cells by internalizing quantum dots. (2009)
  • Author(s): Ohyabu, Y., Kaul, Z., Yoshioka, T., Inoue, K., Sakai, S., Mishima, H., Uemura, T., Kaul, S.C., Wadhwa, R.
  • Journal Title: Human Gene Therapy 20 Pages: 1-7
  • Peer Reviewed
• [Journal Article] The versatile stress protein mortalin as a chaperone therapeutic agent. (2009)
  • Author(s): Deocaris, C.C., Kaul, S.C., Wadhwa, R.
  • Journal Title: Protein & Peptide Lett. 16 Pages: 517-529
  • Peer Reviewed
• [Journal Article] Stable and non-invasive illumination of mesenchymal stem cells by internalizing quantum dots (2008)
  • Author(s): Y. Ohyabu, Z. Kaul, T. Yoshioka, S. Sakai, H. Mishima, T. Uemuura, S. Kaul, R. Wadwha
  • Journal Title: HUMAN GENE THERAPY 20 Pages: 210-226
  • Peer Reviewed
• [Journal Article] Internalizing antibody-based targeted gene delivery for human cancer cells. (2007)
  • Author(s): Shiota, M., Ikeda, Y., Kaul, Z., Itadani, J., Kaul, S.C., Wadhwa, R
  • Journal Title: Human Gene Therapy 18 Pages: 1153-1160
  • Peer Reviewed
• [Journal Article] Quantum dot-based mortalin staining as a visual assay for detection of induced senescence in cancer cells. (2007)
  • Author(s): Kaul, Z., Yaguchi, T., Chiura, H.X., Kaul, S.C., Wadhwa, R
  • Journal Title: Ann. N.Y. Acad. Sci 1100 Pages: 368-372
  • Peer Reviewed
• [Journal Article] An antibody-conjugated internalizing quantum dot suitable for long-term live imaging of cells. (2007)
  • Author(s): Kaul, Z., Yaguchi T., Harada J.I., Ikeda Y., Hirano, T., Chiura H.X., Kaul, S.C., Wadhwa, R
  • Journal Title: Biochem. Cell Biol 85 Pages: 133-140
  • Peer Reviewed
• [Journal Article] An antibody-conjugated internalizing quantum dot suhable for long-term live imagingof cells. (2007)
  • Author(s): Kaul, Z., Yaguchi, T., Harada, J.I., Ikeda, Y., Hirano, T., Chiura, H.X., Kaul, S.C., and Wadhwa, R.
  • Journal Title: Biochein.Cell Biol. 85 Pages: 133-140
  • Peer Reviewed
• [Journal Article] Quantum dot-based mortalin staining as a visual assay fbr detection of inducedSenescence in cancer cells (2007)
  • Author(s): Kaul, Z., Yaguchi, T., Chiura, H.X., Kaul, S.C., and Wadhwa, R.
  • Journal Title: Ann. N.Y. Acad. Sci 1100 Pages: 368-372
  • Peer Reviewed
• [Journal Article] Intemalizing antibody-based targeted gene delivery for human cancer cells (2007)
  • Author(s): Shiota, M., Ikeda, Y., Kaul, Z., Itadani, J., Kaul, S.C., and Wadhwa, R
  • Journal Title: Hum. Gene Ther 18 Pages: 1153-1160
  • Peer Reviewed
• [Presentation] Stress protein mortalin a dual regulator of age diseases, cancers and neurodegeneration. (2009)
  • Author(s): Wadhwa, R
  • Organizer: XXVII Annual conference of Indian Academy of Neurosciences
  • Place of Presentation: NIMS University, India
  • Year and Date: 2009-12-19
• [Presentation] Stress protein mortalin- a dual regulator of age diseases, cancers and neurodegeneration (2009)
  • Author(s): Wadhwa, R.
  • Organizer: XXVII Annual conference of Indian Academy of Neurosciences
  • Place of Presentation: NIMS University(India)
  • Year and Date: 2009-12-19
• [Presentation] Functional Significance of Molecular Interactions Between the anti-apoptotic proteins Bcl-xl, Bcl-2 and Mortalin. (2009)
  • Author(s): Saxena, N., Shreshtha, B.G., Cheung, C., Ishii, T., Wadhwa, R., Kaul, S. C
  • Organizer: Asia Pacific Cancer Conference
  • Place of Presentation: Tsukuba
  • Year and Date: 2009-11-10
• [Presentation] Functional Significance of Molecular Interactions Between the anti-apoptotic proteins Bcl-x1, Bcl-2 and Mortalin (2009)
  • Author(s): Saxena, N., Shreshtha, B.G., Cheung, C., Ishii, T., Wadhwa, R., Kaul, S.C.
  • Organizer: Asia Pacific Cancer Conference
  • Place of Presentation: つくば国際会議場
  • Year and Date: 2009-11-10
• [Presentation] Studies on the control of proliferation and its intervention in human normal and cancer cells. (2009)
  • Author(s): Wadhwa, R
  • Organizer: 3rd Japan (AIST)-India (DBT) Symposium on Glycoscience, Bioinformatics & Cell Engineering
  • Place of Presentation: AIST
  • Year and Date: 2009-10-27
• [Presentation] Studies on the control of proliferation and its intervention in human normal and cancer cells (2009)
  • Author(s): Wadhwa, R.
  • Organizer: 3^<rd> Japan(AIST)-India(DBT)Symposium on Glycoscience, Bioinformatics & Cell Engineering
  • Place of Presentation: 産業施術総合研究所
  • Year and Date: 2009-10-27
• [Presentation] Mortalin-Bcl-2 Interactions Acts as a Tumor Suppressor Mechanism in Cancer Cells. (2009)
  • Author(s): Saxena, N., Shreshtha, B.G., Ishii, T., Kaul, S.C., Wadhwa, R
  • Organizer: 2nd China - Japan Graduate Students Forum entitled "Life, Energy and Environment"
  • Place of Presentation: Beijing, China
  • Year and Date: 2009-10-24
• [Presentation] Mortalin-Bcl-2 Interactions Acts as a Tumor Suppressor Mechanism in Cancer Cells (2009)
  • Author(s): Saxena, N., Shreshtha, B.G., Ishii, T., Kaul, S.C., Wadhwa, R.
  • Organizer: 2nd China-Japan Graduats Students Forum entitled "Life, Energy and Environment"
  • Place of Presentation: Beijing(China)
  • Year and Date: 2009-10-24
• [Presentation] Mortalin/mthsp70 dampens down the activities of tumor suppressor protein p53 in human cancers. (2009)
  • Author(s): Wadhwa, R
  • Organizer: The Fourth International Congress on Stress Response in Biology and Medicine
  • Place of Presentation: Sapporo
  • Year and Date: 2009-10-07
• [Presentation] Mortalin translocates from mitochondria to nucleus and promotes human tumorigenesis. (2009)
  • Author(s): Yaguchi, T., Ryu, J., Yun, A., Yun, C-Ok., Wadhwa, R., Kaul, S. C
  • Organizer: The Fourth International Congress on Stress Response in Biology and Medicine
  • Place of Presentation: Sapporo
  • Year and Date: 2009-10-07
• [Presentation] Mortalin/mthsp70 dampens down the activities of tumor suppressor protein p53 in human cancers. (2009)
  • Author(s): Wadhwa, R.
  • Organizer: The Fourth International Congress on Stress Response in Biology and Medicine
  • Place of Presentation: ガトーキングダムサッポロ
  • Year and Date: 2009-10-07
• [Presentation] Mortalin translocates from mitochondria to nucleus and promotes human tumorigenesis (2009)
  • Author(s): Yaguchi, T., Ryu, J., Yoon, A., Yun, C-Ok., Wadhwa, R., Kaul, S.C.
  • Organizer: The Fourth International Congress on Stress Response in Biology and Medicine
  • Place of Presentation: ガトーキングダムサッポロ
  • Year and Date: 2009-10-07
• [Presentation] Stress protein mortalin - based cancer therapeutics. AIST - CNRS bilateral workshop in life sciences. (2009)
  • Author(s): Wadhwa, R
  • Organizer: Institute of Biological Sciences
  • Place of Presentation: CNRS, Paris
  • Year and Date: 2009-09-24
• [Presentation] Stress protein mortalin-based cancer therapeutics. (2009)
  • Author(s): Wadhwa, R.
  • Organizer: AIST-CNRS bilateral workshop in life sciences. Institute of Biological Sciences
  • Place of Presentation: CNRS(Paris)
  • Year and Date: 2009-09-24
• [Presentation] Mortalin based cancer diagnostics and therapeutics. (2009)
  • Author(s): Wadhwa, R
  • Organizer: Biomarkers for early detection of cancers: Technological advances and clinical readiness
  • Place of Presentation: Cheung Kung Hai Conference Center, Hong Kong
  • Year and Date: 2009-04-02
• [Presentation] Mortalin based cancer diagnostics and therapeutics. (2009)
  • Author(s): Wadhwa, R.
  • Organizer: Biomarkers for early detection of cancers : Technological advances and clinical readiness.
  • Place of Presentation: Cheung Kung Hai Conference Center(Hong Kong)
  • Year and Date: 2009-04-02
• [Presentation] Sunil Kaul, Renu Wadhwa, モータリン抗体を用いたQuantum-dot導入による間葉系幹細胞のin vitro/in vivoラベリング (2008)
  • Author(s): 大藪淑美, Zeenia Kaul, 吉岡友和, 井上和貴, 酒井晋介, 三島初, 植村寿公
  • Organizer: BMB2008(第31回日本分子生物学会年会・第81回日本生化学会大会合同大会)
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2008-12-12
• [Presentation] モータリン抗体を用いた Quantum-dot 導入による間葉系幹細胞の in vitro/in vivo ラベリング (2008)
  • Author(s): 大藪淑美, Zeenia Kaul, 吉岡友和, 井上和貴, 酒井晋介, 三島初, 植村寿公, Sunil Kaul, Renu Wadhwa
  • Organizer: BMB2008(第31回日本分子生物学会年会・第8回日本生化学会大会合同大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2008-12-12
• [Presentation] Use of internalizing Quantum dots (iQdots) for cell tracking, cancer diagnosis and in vivo imaging. (2008)
  • Author(s): Kaul, Z., Shiota, M., Kaul, S.C., Wadhwa, R., Reddel, R
  • Organizer: Sydney Cancer Conference 2008
  • Place of Presentation: The University of Sydney, Australia
  • Year and Date: 2008-07-24
• [Presentation] Use of internalizing Quantum dots (iQdots) for cell tracking, cancer diagnosis and in vivo imaging (2008)
  • Author(s): Kaul, Z., Shiota, M., Kaul, S. C., Wadhwa, R., Reddel, R.
  • Organizer: Sydney Cancer Conference 2008
  • Place of Presentation: The University of Sydney, Australia
• [Presentation] Use of quantum dots for in vivo imaging, cell tracking and cancer diagnostics. (2007)
  • Author(s): Kaul, Z., Yaguchi, T., Kaul, S.C., Wadhwa, R
  • Organizer: SENECA 2007
  • Place of Presentation: European Conference on Cancer and Ageing. Warsaw, Poland
  • Year and Date: 2007-10-04
• [Presentation] Quantum dots for senescence inducing si-RNA screening and in vivo imaging. (2007)
  • Author(s): Kaul, Z., Yaguchi, T., Shiota, M., Chiura, H.X., Kaul, S.C., Wadhwa, R
  • Organizer: 12th Congress of the International Association of Biomedical Gerontology (I.A.B. G)
  • Place of Presentation: Greece
  • Year and Date: 2007-05-20
• [Presentation] Generation of illuminating cells by novel internalizing antibody conjugated quantum dots. (2007)
  • Author(s): Kaul, Z., Yaguchi, T., Chiura, H.X., Kaul, S.C., Wadhwa, R
  • Organizer: 80th Japanese Tissue Culture Association Meeting, Life Science Center
  • Place of Presentation: Osaka
  • Year and Date: 2007-05-14
• [Presentation] Use of qumtum dots for in vivo imaging,cell tracking and cancer diagnostics (2007)
  • Author(s): Kaul, Z., Yaguchi, T., Kaul, S.C., and Wadhwa, R
  • Organizer: SENECA2007.European Conference on Cancer and Ageing
  • Place of Presentation: Warsaw, Poland
• [Presentation] Quantum dots for senescence inducing si-RNA screening and in vivo imaging (2007)
  • Author(s): Kaul, Z., Yaguchi, T., Shiota, M., Chiura, H.X., Kaul, S.C., and Wadhwa, R.
  • Organizer: 12th Congress of the International Association of Biomedical Gerontology(I.A.B, G)
  • Place of Presentation: Spetses Island, Greece
• [Presentation] Geneiation of illuminating cells by novel internalizing antibody conjugated quantum dots (2007)
  • Author(s): Kaul, Z., Yaguchi, T., Chiura, H, X., Kaul, S.C., and Wadhwa, R.
  • Organizer: 80th Japanese Tissue Culture Association Meeting
  • Place of Presentation: Life Science Center, Osaka