Is an increase in GLUT4 mRNA in response to exercise training mediated by MEF2?

• Project/Area Number: 19300225
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Sports science
• Research Institution: National Institute of Health and Nutrition
• Principal Investigator: EZAKI Osamu National Institute of Health and Nutrition, 基礎栄養プログラム, プログラムリーダー (90191923)
• Co-Investigator(Kenkyū-buntansha): 三浦 進司 独立行政法人国立健康・栄養研究所, プロジェクトリーダー (10342932)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000); Fiscal Year 2009: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000); Fiscal Year 2008: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000); Fiscal Year 2007: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
• Keywords: インスリン抵抗性 / GLUT4 / 運動 / 高脂肪食 / トランスジェニックマウス / 筋肉 / 脂肪組織 / 遺伝子 / 生体分子 / 糖尿病 / 発現制御 / バイオテクノロジー

Research Abstract

To examine whether MEF2 was involved in an increase in GLUT4 mRNA by exercise training, MEF2 overexpressed transgenic mice in skeletal muscles were produced. MEF2 overexpressed mice did not increase GLUT4 mRNA in skeletal muscles. Transcription co-activators, PGC-1α-b and PGC-1α-c, are increased by exercise training and activate MEF2. However, PGC-1α-b or PGC-1α-c overexpressed mice did not increase GLUT4 mRNA in skeletal muscles. These data suggested that MEF2 was not involved in an increase in GLUT4 mRNA by exercise training.

Research Products

• [Journal Article] The cathepsin L gene is a direct target of FOXO1 in skeletal muscle. (2010)
  • Author(s): Yamazaki Y, Kamei Y, Sugita S, Akaike F, Kanai S, Miura S, Hirata Y, Troen BR, Kitamura T, Nishino I, Suganami T, Ezaki O, Ogawa Y
  • Journal Title: Biochem J. 427 Pages: 171-178
• [Journal Article] Increased expression of DNA methyltransferase 3a in obese adipose tissue: studies with transgenic mice. (2010)
  • Author(s): Kamei Y, Suganami T, Ehara T, Kanai S, Hayashi K, Yamamoto Y, Miura S, Ezaki O, Okano M, Ogawa Y
  • Journal Title: Obesity (Silver Spring) 18 Pages: 314-321
• [Journal Article] Increased expression of DNA methyltransferase 3a in obese adipose tissue : studies with transgenic mice. (2010)
  • Author(s): Suganami T, Ehara T, Kanai S, Hayashi K, Yamamoto Y, Miura S, Ezaki O, Okano M, Ogawa Y.
  • Journal Title: Obesity(Silver Spring). 18 Pages: 314-321
  • Peer Reviewed
• [Journal Article] The cathepsin L gene is a direct target of FOXO1 in the skeleta 1 muscle. (2010)
  • Author(s): Yamazaki Y, Kamei Y, Sugita S, Akaike F, Kanai S, Miura S, Hirata Y, Troen BR, Kitamura T, Nishino I, Suganami T, Ezaki O, Ogawa Y.
  • Journal Title: Biochem J. 427 Pages: 171-178
  • Peer Reviewed
• [Journal Article] (2010)
  • Author(s): 河盛隆造・岩本安彦編集江崎治
  • Journal Title: 糖尿病最新の治療2010-2012(南江堂) Pages: 99-104
• [Journal Article] Ubiquitin C-terminal hydrolase-L3-Knockout mice are resistant to diet-induced obesity and show increased activation of AMP-activated protein kinase in skeletal muscle. (2009)
  • Author(s): Setsuie R, Suzuki M, Kabuta T, Fujita H, Miura S, Ichihara N, Yamada D, Wang YL, Ezaki O, Suzuki Y, Wada K
  • Journal Title: FASEB J. 23 Pages: 4148-4157
• [Journal Article] Overexpression of FOXO1 in skeletal muscle does not alter longevity in mice. (2009)
  • Author(s): Chiba T, Kamei Y, Shimizu T, Shirasawa T, Katsumata A, Shiraishi L, Sugita S, Ogawa Y, Miura S, Ezaki O
  • Journal Title: Mech Ageing Dev 130 Pages: 420-428
• [Journal Article] α2-AMPK activity is not essential for an increase in fatty acid oxidation during low-intensity exercise. (2009)
  • Author(s): Miura S, Kai Y, Kamei Y, Bruce CR, Kubota N, Febbraio MA, Kadowaki T, Ezaki O
  • Journal Title: Am J Physiol Endocrinol Metab 296
• [Journal Article] Overexpression of FOXO1 in skeletal muscle does not alter longevity in mice. (2009)
  • Author(s): Chiba T, Kamei Y, Shimizu T, Shirasawa T, Katsumata A, Shiraishi L, Sugita S, Ogawa Y, Miura S, Ezaki O.
  • Journal Title: Mech Ageing Dev. 130 Pages: 420-428
  • Peer Reviewed
• [Journal Article] Ubiquitin C-terminal hydrolase-L3-knockout mice are resistant to diet-induced obesity and show increased activation of AMP-activated protein kinase in skeletal muscle. (2009)
  • Author(s): Setsuie R, Suzuki M, Kabuta T, Fujita H, Miura S, Ichihara N, Yamada D, Wang YL, Ezaki O, Suzuki Y, Wada K.
  • Journal Title: FASEB J. 23 Pages: 4148-4157
  • Peer Reviewed
• [Journal Article] α2-AMPK activity is not essential for an increase in fatty acid oxidation during low-intensity exercise (2009)
  • Author(s): Miura S, Kai Y, Kamei Y, Bruce CR, Kubota N, Febbraio MA, Kadowaki T, Ezaki O.
  • Journal Title: Am J Physiol Endocrinol Metab 296
  • Peer Reviewed
• [Journal Article] Isoform-Specific Increases in Murine Skeletal Muscle Peroxisome Proliferator-Activated Receptor-γCoactivator-1α(PGC-1α)mRNA in Responseto 2-Adrenergic Receptor Activation and Exercise. (2008)
  • Author(s): Miura S, Kai Y, Kamei Y, Ezaki O
  • Journal Title: Endocrinology 149 Pages: 4527-4533
• [Journal Article] Isoform-Specific Increases in Murine Skeletal Muscle Peroxisome Proliferator-Activated Receptor-Y Coactivator-1α (PGC-1α) mRNA in Response to 2-Adrenergic Receptor Activation and Exercise (2008)
  • Author(s): Miura S, Kai Y, Kamei Y, Ezaki O.
  • Journal Title: Endocrinology 149 Pages: 4527-4533
  • Peer Reviewed
• [Journal Article] An increase in murine skeletal muscle peroxisome proliferators-activated receptor-γcoactivator-1α(PGC-1α) mRNA in response to exercise is mediated byα-adrenergic receptor activation. (2007)
  • Author(s): Miura S, Kawanaka K, Kai Y, Tamura M, Goto M, Shiuchi T, Minokoshi Y, Ezaki O
  • Journal Title: Endocrinology 148 Pages: 3441-3448
• [Journal Article] An increase in murine skeletal muscle peroxisome proliferator-activatedreceptor-γ coactivator-1α(PGC-1α)mRNA in response to exercise is mediated by β-adrenergic-receptor activation (2007)
  • Author(s): Miura S, Kawanaka K, Kai Y, Tamura M, Goto M, Shiuchi T, Minokoshi Y, Ezaki O.
  • Journal Title: Endoerinology 148 Pages: 3441-3448
  • Peer Reviewed
• [Presentation] 骨格筋特異的RXRγ過剰発現マウスは糖代謝改善作用を示す (2009)
  • Author(s): 杉田聡, 亀井康富, 赤池史子, 菅波孝洋, 金井紗綾香, 三浦進司, 江崎治, 小川佳宏
  • Organizer: 第30回日本肥満学会
  • Place of Presentation: アクトシティ浜松(静岡)
  • Year and Date: 2009-10-09
• [Presentation] 肥満とDNAメチル化:DNAメチル化酵素遺伝子改変マウスとメチル化促進飼料による検討 (2009)
  • Author(s): 亀井康富, 菅波孝祥, 江原達弥, 金井沙綾香, 三浦進司, 江崎治, 小川佳宏
  • Organizer: 第30回日本肥満学会
  • Place of Presentation: アクトシティー浜松(浜松市)
  • Year and Date: 2009-10-09
• [Presentation] 骨格筋におけるFOXO1によるタンパク分解酵素カテプシンL遺伝子の転写調節 (2009)
  • Author(s): 山崎芳浩, 亀井康富, 三浦進司, 杉田聡, 赤池史子, 金井沙綾香, 平田結喜緒, 江崎治, 菅波孝祥, 小川佳宏
  • Organizer: 第30回日本肥満学会
  • Place of Presentation: アクトシティー浜松(浜松市)
  • Year and Date: 2009-10-09
• [Presentation] 骨格筋特異的RXRγ過剰発現マウスは糖代謝改善作用を示す (2009)
  • Author(s): 杉田聡、亀井康富、赤池史子、菅波孝洋、金井紗綾香、三浦進司、江崎治、小川佳宏
  • Organizer: 第30回 日本肥満学会
  • Place of Presentation: 静岡
  • Year and Date: 2009-10-09
• [Presentation] 肥満とDNAメチル化:DNAメチル化酵素遺伝子改変マウスとメチル化促進飼料による検討 (2009)
  • Author(s): 亀井康富、菅波孝祥、江原達弥、金井沙綾香、三浦進司、江崎治、小川佳宏
  • Organizer: 第30回 日本肥満学会
  • Place of Presentation: 静岡
  • Year and Date: 2009-10-09
• [Presentation] 骨格筋におけるFOXO1によるタンパク分解酵素カテプシンL遺伝子の転写調節 (2009)
  • Author(s): 山崎芳浩、亀井康富、三浦進司、杉田聡、赤池史子、金井沙綾香、平田結喜緒、江崎治、菅波孝祥、小川佳宏
  • Organizer: 第30回 日本肥満学会
  • Place of Presentation: 静岡
  • Year and Date: 2009-10-09
• [Presentation] QOLの視点から望ましい食事と運動の姿を考える (2009)
  • Author(s): 江崎治
  • Organizer: 第10回日本QOL学会
  • Place of Presentation: 独立行政法人国立健康・栄養研究所(東京)
  • Year and Date: 2009-09-27
• [Presentation] QOLの視点から望ましい食事と運動の姿を考える (2009)
  • Author(s): 江崎治
  • Organizer: 第10回 日本QOL学会
  • Place of Presentation: 東京
  • Year and Date: 2009-09-27
• [Presentation] Fibrate treatment causes muscle atrophy with increased FOXO1 mRNA in a PPARα-dependent manner in mice. (2009)
  • Author(s): Miura S, Kai Y, Ezaki O
  • Organizer: The 69th American Diabetes Association Scientific Sessions
  • Place of Presentation: New Orleans, LA, USA
  • Year and Date: 2009-06-06
• [Presentation] Fibrate treatment causes muscle atrophy with increased FOXO1 mRNA in a PPARα-dependent manner in mice. (2009)
  • Author(s): Miura S, Kai Y, Ezaki O.
  • Organizer: The 69th American Diabetes Association Scientific Sessions.
  • Place of Presentation: New Orleans, LA, USA.
  • Year and Date: 2009-06-06
• [Book] 糖尿病最新の治療2010-2012. 運動療法の効果と実際, 注意点 (2010)
  • Author(s): 河盛隆造・岩本安彦編集江崎治
  • Publisher: 南江堂