Role of LRP5 in various metabolic pathways
Project/Area Number |
19300227
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Applied health science
|
Research Institution | Tohoku University |
Principal Investigator |
YAMAMOTO Tokuo Tohoku University, 加齢医学研究所, 教授 (30192412)
|
Project Period (FY) |
2007 – 2009
|
Project Status |
Completed (Fiscal Year 2009)
|
Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2009: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2008: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2007: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
|
Keywords | 生活習慣病 / コレステロール / 糖尿病 / LPR5 / Wnt / LRP5 |
Research Abstract |
In previous studies, we showed that the deficiency of LRP5, an LDL receptor related protein, impairs various metabolic pathways including cholesterol, glucose and calcium. We also showed that the lack of LRP5 enhances the formation of atherosclerosis, using mice lacking both apoE and LRP5. To further clarify the role of LRP5 in these metabolic disorders, we purified various agonists and antagonists of LRP5, including Wnt proteins, Dickopf 1-3, soluble frizzled related proteins, soluble LRP5 and LRP6 lacking membrane domains. Purification of Wnt proteins without detergents was extremely difficult because they are modified with palmitate. Nusse and his colleges purified Wnt3a using Triton X-100 and CHAPS, showing that Wnts proteins are modified with palmitate, thereby exhibiting extremely hydrophobic character. In this study, I purified Wnt3a、 Wnt 5a and Wnt4 without using detergents and characterized. The purification and characterization of these proteins will be published.
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Report
(4 results)
Research Products
(6 results)
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[Journal Article] Fasting-induced hypothermia and reduced energy production in mice lacking acetyl-CoA synthetase 22009
Author(s)
Sakakibara I, Fujino T, Ishii M, Tanaka T, Shimosawa T, Miura S, Zhang W, Tokutake Y, Yamamoto J, Awano M, Iwasaki S, Motoike T, Okamura M, Inagaki T, Kita K, Ezaki O, Naito M, Kuwaki T, Chohnan S, Yamamoto TT, Hammer RE, Kodama T, Yanagisawa M, Sakai J
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Journal Title
Cell Metab 9(2)
Pages: 191-202
Related Report
Peer Reviewed
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[Journal Article] Fasting-induced hypothermia and reduced energy production in mice lacking acetyl-CoA synthetase 2.2009
Author(s)
Sakakibara I, Fujino T, Ishii M, Tanaka T, Shimosawa T, Miura S, Zhang W, Tokutake Y, Yamamoto J, Awano M, Iwasaki S, Motoike T, Okamura M, Inagaki T, Kita K, Ezaki O, Naito M, Kuwaki T, Chohnan S, Yamamoto TT, Hammer RE, Kodama T, Yanagisawa M, Sakai J.
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Journal Title
Cell Metab 9
Pages: 191-202
Related Report
Peer Reviewed
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[Journal Article] Fasting-Induced hypothermia and reduced energy production in mice lacking acetyl-CoA synthetase 22009
Author(s)
Sakakibara I, Fujino T, Ishii M, Tanaka T, Shimosawa T, Miura S, Zhang W, Tokutake Y, Yamamoto J, Awano M, Iwasaki S, Motoike T, Okamura M, Inagaki T, Kita K, Ezaki 0, Naito M, Kuwaki T, Chohnan S, Yamamoto TT, Hammer RE, Kodama T, Yanagisawa M, Sa kai J
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Journal Title
Cell Metabolism 9(2)
Pages: 191-202
Related Report
Peer Reviewed
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[Journal Article] SOX6 suppresses cyclin D1 promoter activity by interacting with beta-catenin and histone deacetylase 1, and its down-regulation induces pancreatic beta-cell proliferation2007
Author(s)
Iguchi H, Urashima Y, Inagaki Y, Ikeda Y, Okamura M, Tanaka T, Uchida A, Yamamoto TT, Kodama T, Sakai J
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Journal Title
J Biol Chem 282
Pages: 19052-19061
Related Report
Peer Reviewed
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[Journal Article] Involvement of apolipoprotein E in excess fat accumulation and insulin resistance2007
Author(s)
Gao J, Katagiri H, Ishigaki Y, Yamada T, Ogihara T, Imai J, Uno K, Hasegawa Y, Kanzaki M, Yamamoto TT, Ishibashi S, Oka Y
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Journal Title
Related Report
Peer Reviewed
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[Journal Article] SOX6 Suppresses Cyclin D1 Promoter Activity by Interacting with β-Ctenin and Histone Deacetylase 1, and Its Down-regulation Induces Pancreatic β-Cell Proliferation.2007
Author(s)
Iguchi H, Urashima Y, Inagaki Y, Ikeda Y, Okamura M, Tanaka T, Uchida A, Yamamoto TT, Kodama T, Sakai J
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Journal Title
Journal of Biological Chemistry 282(26)
Pages: 19052-19061
Related Report
Peer Reviewed