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Application of taste modifying protein, neoculin and miraculin for food preperation

Research Project

Project/Area Number 19300248
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Eating habits, studies on eating habits
Research InstitutionThe University of Tokyo

Principal Investigator

ASAKURA Tomiko  The University of Tokyo, 大学院・農学生命科学研究科, 特任准教授 (20259013)

Co-Investigator(Kenkyū-buntansha) 南 道子  東京学芸大学, 教育学部, 教授 (70272432)
舟木 淳子  福岡女子大学, 人間環境学部, 准教授 (60219079)
岡田 晋治  東京大学, 大学院・農学生命科学研究科, 助教 (50376563)
Co-Investigator(Renkei-kenkyūsha) MINAMI Michiko  東京学芸大学, 教育学部, 教授 (70272432)
FUNAKI Junko  福岡女子大学, 人間環境学部, 准教授 (60219029)
OKADA Shinji  東京大学, 大学院・農学生命科学研究科, 助教 (50376563)
Project Period (FY) 2007 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥9,880,000 (Direct Cost: ¥7,600,000、Indirect Cost: ¥2,280,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2007: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Keywordsネオクリン / ミラクリン / 甘味受容体 / 味覚修飾タンパク質 / 甘味タンパク質
Research Abstract

Neoculin and miraculin are taste modifying proteins which convert sourness to sweetness. They are occurring in tropical fruits, Curculigo latiforia and Synsepalum dulcificum, respectively. Each native protein is difficult to obtain constantly. We succeeded to produce recombinant neoculin and miraculin with Aspergillus oryzae that was designated to GRAS. To verify the safety of neoculin, viability of Caco-2 cells exposed to neoculin was investigated. The viability of cells was not changed with neoculin exposure. DNA microarray analysis also revealed that neoculin did not affect the gene expression of Caco-2 cells. Neoculin is received by human sweet taste receptor, T1R2-T1R3. The interaction sites of neoculin to T1R2-T1R3 were analyzed using HEK293T cells that express T1R2-T1R3. As a result, neoculin was received at the N-terminal extra cellar domain in T1R3.

Report

(4 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • 2007 Annual Research Report
  • Research Products

    (51 results)

All 2010 2009 2008 2007 2005 2004 Other

All Journal Article (25 results) (of which Peer Reviewed: 15 results) Presentation (13 results) Book (11 results) Remarks (1 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] pH-dependent Structural Change of Neoculin in Special Reference to Its Taste-modifying2009

    • Author(s)
      Morita, Y., Nakajima, K., Iizuka, K., Terada, T., Shimizu-Ibuka, A., Ito, K., Koizumi, A., Asakura, T., Misaka, T., Abe, K.
    • Journal Title

      Activity. Biosci. Biotech. Biochem. 73

      Pages: 2552-2555

    • Related Report
      2009 Final Research Report
  • [Journal Article] pH-Dependent Inhibition of the Human Bitter Taste Receptor hTAS2R16 by a Variety of Acidic Substances2009

    • Author(s)
      Sakurai, T., Misaka,T., Nagai, T., Ishimaru, Y., Matsuo, S., Asakura, T., Abe, K.
    • Journal Title

      J.Agric.Food Chem. 57

      Pages: 2508-25141

    • Related Report
      2009 Final Research Report
  • [Journal Article] Relationship between tissue structural collapse and disappearance of flesh transparency during postmortem changes in squid mantles.2009

    • Author(s)
      Kugino, M.
    • Journal Title

      J.Food.Sci. 74

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] PH-dependent structural change in neoculin with special reference to its taste-modifying activity.2009

    • Author(s)
      Morita Y.
    • Journal Title

      Biosci.Biotechnol.Biochem. 73

      Pages: 2552-2555

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Signal peptide peptidases are expressed in the shoot apex of rice, localized to the endoplasmic reticulum.2009

    • Author(s)
      Tamura, T.
    • Journal Title

      Plant Cell Rep. 28

      Pages: 1615-1621

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cathepsin D-like aspartic proteinase occurring in a maize weevil, Sitophilus zeamais, as its candidate of digestive enzyme.2009

    • Author(s)
      Mstsumoto, I
    • Journal Title

      Biosci.Biotechnol.Biochem. 73

      Pages: 2338-2340

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] pH-Dependent Inhibition of the Human Bitter Taste Receptor hTAS2R16 by a Variety of Acidic Substances.2009

    • Author(s)
      Sakurai, T.
    • Journal Title

      J.Agric.Food chem. 57

      Pages: 2508-2514

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Wheat cysteine proteases triticain a, b, and c exhibit mutually distinct responses to gibberellin in germinating seeds.2009

    • Author(s)
      Kiyosaki, T.
    • Journal Title

      J.Plant physiol. 166

      Pages: 101-106

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 味覚修飾タンパク質ネオクリンの活性化機構と新規甘味タンパク質の開発2009

    • Author(s)
      中島健一朗
    • Journal Title

      蛋白質核酸酵素 54

      Pages: 843-848

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Neoculin a taste-modifying sweet protein, accumulates in ripening fruits of cultivated Curculigo latifolia2008

    • Author(s)
      Okubo, S., Asakura, T., Okubo, K., Abe, K., Misaka, T., Akita, T., Abe K.
    • Journal Title

      J.Plant Physiol. 165(18)

      Pages: 1964-9

    • Related Report
      2009 Final Research Report
  • [Journal Article] Biochemical and Genomic Analysis of Neoculin Compared to Monocot Mannose-Binding Lectins2008

    • Author(s)
      Shimizu-Ibuka, A., Nakai, Y., Nakamori, K., Morita, Y., Nakajima, K., Kadota, K., Watanabe, H., Okubo, S., Terada, T., Asakura, T., Misaka, T., Abe, K.
    • Journal Title

      J.Agric.Food Chem. 56

      Pages: 5338-5344

    • Related Report
      2009 Final Research Report
  • [Journal Article] Acid-induced sweetness of neoculin is ascribed to its pH-dependent agonistic-antagonistic interaction with human sweet taste receptor2008

    • Author(s)
      Nakajima, K., Morita, Y., Koizumi, A., Asakura, T., Terada, T., Ito, K., Shimizu-Ibuka, A., Maruyama, J., Kitamoto, K., Misaka, T., Abe K.
    • Journal Title

      FASEB J. 22

      Pages: 2323-2330

    • Related Report
      2009 Final Research Report
  • [Journal Article] Signal peptide peptidase and its homologs in Arabidopsis thaliana-plant tissue-specific expression and distinct subcellular localization2008

    • Author(s)
      Tamura, T., Asakura, T., Uemura, T., Ueda, T., Terauchi, K., Misaka, T., Abe, K.
    • Journal Title

      FEBS J. 275

      Pages: 34-43

    • Related Report
      2009 Final Research Report
  • [Journal Article] Signal peptide peptidase and its homologs in Arabidopsis thaliana-planttissue-specific expression and distinct subcellular localization.2008

    • Author(s)
      Tamura, T
    • Journal Title

      FEBS J. 275

      Pages: 34-43

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Application of an Aspergillus saitoi protease preparation to soybean curd to modify its functional and rheological properties2008

    • Author(s)
      Nishinoaki, M.
    • Journal Title

      Biosci. Biotech. Biochem. 72

      Pages: 587-590

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Biochemical and Genomic Analysis of Neoculin Compared to Monocot Mannose-Binding Lectins.2008

    • Author(s)
      Shimizu-Ibuka, A.
    • Journal Title

      J. Agric. Food Chem. 56

      Pages: 5338-5344

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Acid-induced sweetness of neoculin is ascribed to its pH-dependent agonistic-antagonistic interaction with human sweet taste receptor.2008

    • Author(s)
      Nakajima, K.
    • Journal Title

      FASEB J. 22

      Pages: 2323-2330

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Neoculin, a taste-modifying sweet protein, accumulates in ripening fruits of cultivated Curculigo latifolia2008

    • Author(s)
      Okubo, S.
    • Journal Title

      J. Plant Physiol. 165

      Pages: 1964-1969

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Microbial production of sensory-active miraculin2007

    • Author(s)
      Ito, K., Asakura, T., Morita, Y., Nakajima, K., Koizumi, A., Shimizu-Ibuka, A., Masuda, K., Ishiguro, M., Terada, T., Maruyama, J., Kitamoto, K., Misaka, T., Abe, K.
    • Journal Title

      Biochem. Biophys. Res. Commun. 360

      Pages: 407-411

    • Related Report
      2009 Final Research Report
  • [Journal Article] Taste-modifying sweet protein, neoculin, is received at human T1R3 amino terminal domain2007

    • Author(s)
      Koizumi, A., Nakajima, K., Asakura, T., Morita, Y., Ito, K., Shmizu-Ibuka, A., Misaka, T., Abe, K.
    • Journal Title

      Biochem. Biophys. Res. Commun. 358

      Pages: 585-589

    • Related Report
      2009 Final Research Report
  • [Journal Article] Gliadain, a gibberellin-inducible cysteine proteinase occurring in germinating seeds of wheat, Triticum aestivum L., specifically digests gliadin and is regulated by intrinsic cystatins2007

    • Author(s)
      Kiyosaki, T., Matsumoto, I., Asakura, T., Funaki, J., Kuroda, M., Misaka, T., Arai, S., Abe, K.
    • Journal Title

      FEBS J. 274

      Pages: 1908-1917

    • Related Report
      2009 Final Research Report
  • [Journal Article] Differential functions of wheat aspartic proteinases, WAP1 and WAP2, in germinating and maturing seeds2007

    • Author(s)
      Tamura, T., Terauchi, K., Kiyosaki, T., Asakura, T., Funaki, J., Matsumoto, I., Misaka, T., Abe, K.
    • Journal Title

      J.Plant Physiol 164

      Pages: 470-477

    • Related Report
      2009 Final Research Report
  • [Journal Article] Taste-modifying sweet protein,neoculin,is received at human TIR3 amino temlinal domain2007

    • Author(s)
      Koizumi.A, Nakajima, K., Asakura, T., Morita, Y., lto, K., Shmizu-lbuka, A., Misaka, T., and Abe, K.
    • Journal Title

      Biochem.Biophys.Res.Commun 358

      Pages: 585-589

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Microbial production of senspry-active miraculin2007

    • Author(s)
      Ito, K., Asakura, T., Morita, Y., Nakajima, K., Koizumi, A., Shimizu-Ibuka, A., MasudaK., Ishiguro, M.,
    • Journal Title

      Biochem.Biophys.Res.Commun 360

      Pages: 407-411

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Gliadain,a gibberelin-inducible cysteine proteinase occurring in germinating seeds of whea2007

    • Author(s)
      Kiyosaki, T., Matsumoto, I., Asakura
    • Journal Title

      FEBS J 274

      Pages: 1908-1917

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] 味覚修飾タンパク質ネオクリンの構造、機能相関の分光学的解析2010

    • Author(s)
      小泉太一
    • Organizer
      日本農芸化学会
    • Place of Presentation
      東京大学
    • Year and Date
      2010-03-28
    • Related Report
      2009 Annual Research Report
  • [Presentation] 味覚修飾タンパク質ネオクリンとそのバリアントの培養細胞を用いた活性評価2010

    • Author(s)
      中島健一朗
    • Organizer
      日本農芸化学会
    • Place of Presentation
      東京大学
    • Year and Date
      2010-03-28
    • Related Report
      2009 Annual Research Report
  • [Presentation] 培養細胞を用いた味覚修飾タンパク質ミラクリンとヒト甘味受容体との相互作用の解析2010

    • Author(s)
      古泉文子
    • Organizer
      日本農芸化学会
    • Place of Presentation
      東京大学
    • Year and Date
      2010-03-28
    • Related Report
      2009 Annual Research Report
  • [Presentation] 甘味受容体遺伝子のSNPsとミラクリン感受性の関連2010

    • Author(s)
      土屋麻美
    • Organizer
      日本農芸化学会
    • Place of Presentation
      東京大学
    • Year and Date
      2010-03-28
    • Related Report
      2009 Annual Research Report
  • [Presentation] 施肥量の異なる区で栽培されたイネ種子における遺伝子発現変動の網羅的解析2010

    • Author(s)
      〓川知世
    • Organizer
      日本農芸化学会
    • Place of Presentation
      東京大学
    • Year and Date
      2010-03-28
    • Related Report
      2009 Annual Research Report
  • [Presentation] 表面プラズモン共鳴(SPR)を用いた舌上皮細胞膜モデルと食品成分の相互作用解析2010

    • Author(s)
      宮野元裕
    • Organizer
      日本農芸化学会
    • Place of Presentation
      東京大学
    • Year and Date
      2010-03-28
    • Related Report
      2009 Annual Research Report
  • [Presentation] グリシニン欠失大豆の特性と登熟期における遺伝子発現の網羅的解析2010

    • Author(s)
      田村倫子
    • Organizer
      日本農芸化学会
    • Place of Presentation
      東京大学
    • Year and Date
      2010-03-28
    • Related Report
      2009 Annual Research Report
  • [Presentation] 哺乳類酸味受容体PKDIL3/PKD2L1の構造・機能相関解析2010

    • Author(s)
      片野佑香
    • Organizer
      日本農芸化学会
    • Place of Presentation
      東京大学
    • Year and Date
      2010-03-28
    • Related Report
      2009 Annual Research Report
  • [Presentation] 酸味受容体候補PKD1L3/PKD2L1遺伝子破壊マウスの作出と行動学的表現型解析2010

    • Author(s)
      石丸喜朗
    • Organizer
      日本農芸化学会
    • Place of Presentation
      東京大学
    • Year and Date
      2010-03-28
    • Related Report
      2009 Annual Research Report
  • [Presentation] 酸味情報伝達神経回路の可視化2010

    • Author(s)
      山本くるみ
    • Organizer
      日本農芸化学会
    • Place of Presentation
      東京大学
    • Year and Date
      2010-03-28
    • Related Report
      2009 Annual Research Report
  • [Presentation] ヒト苦味受容体hTAS2R16のリガンド受容部位の解析2010

    • Author(s)
      櫻井敬展
    • Organizer
      日本農芸化学会
    • Place of Presentation
      東京大学
    • Year and Date
      2010-03-28
    • Related Report
      2009 Annual Research Report
  • [Presentation] 酵母を利用したハイスループット変異体作製・解析法の開発と味覚修飾タンパク質ミラクリンへの応用2009

    • Author(s)
      伊藤圭祐
    • Organizer
      日本農芸化学会
    • Place of Presentation
      マリンメッセ福岡
    • Year and Date
      2009-03-28
    • Related Report
      2008 Annual Research Report
  • [Presentation] 味覚修飾タンパク質ネオクリンの構造機能相関の解析2009

    • Author(s)
      中島健一朗
    • Organizer
      日本農芸化学会
    • Place of Presentation
      マリンメッセ福岡
    • Year and Date
      2009-03-28
    • Related Report
      2008 Annual Research Report
  • [Book] 味覚修飾蛋白質ネオクリンの活性化機構と新規甘味蛋白質の開発, 蛋白質核酸酵素2009

    • Author(s)
      中島健一郎, 古泉文子, 朝倉富子, 三坂巧
    • Publisher
      共立出版
    • Related Report
      2009 Final Research Report
  • [Book] 植物のアスパラギン酸プロテアーゼ, 化学と生物2009

    • Author(s)
      朝倉富子, 田村倫子, 寺内かえで, 阿部啓子
    • Publisher
      日本農芸化学会
    • Related Report
      2009 Final Research Report
  • [Book] Neoculin as a novel sweet protein with taste-modifying activity Current Topics in Biotechnology2009

    • Author(s)
      Nakajima, K., Asakura, T., Misaka, T., Abe, K.
    • Related Report
      2009 Final Research Report
  • [Book] 食と味覚(ネスレ栄養科学会議監修)2008

    • Author(s)
      朝倉富子, 阿部啓子, 山本隆, 的場輝佳, ジェーン シュミレット, 共著
    • Publisher
      建帛社
    • Related Report
      2009 Final Research Report
  • [Book] Neoculin as a new sweet protein with taste-modyfying activity : purification, characterization, and X-ray crystallography. Sweetness and Sweeteners, Biology, Chemistry and Psychophysics. ACS symposium series2008

    • Author(s)
      Shimizu-Ibuka, A., Morita, Y., Nakajima, K., Asakura, T., Terada, T., Misaka, T., Abe, K.
    • Related Report
      2009 Final Research Report
  • [Book] 食と味覚2008

    • Author(s)
      朝倉富子
    • Total Pages
      16
    • Publisher
      建帛社
    • Related Report
      2008 Annual Research Report
  • [Book] 機能性食品の事典2007

    • Author(s)
      朝倉富子, 荒井綜一, 阿部啓子, 吉川敏一, 金沢和樹, 渡辺昌, 編集
    • Publisher
      朝倉書店
    • Related Report
      2009 Final Research Report
  • [Book] 機能性食品の事典2007

    • Author(s)
      朝倉 富子
    • Total Pages
      16
    • Publisher
      朝倉書店
    • Related Report
      2007 Annual Research Report
  • [Book] 体験活動に必要な作物ーグリーンアドベンチャー作物編ー2005

    • Author(s)
      朝倉富子
    • Publisher
      国立信州高遠少年自然の家
    • Related Report
      2009 Final Research Report
  • [Book] 調理学実習テキスト2005

    • Author(s)
      朝倉富子, 久木野睦子
    • Publisher
      京大出版センター
    • Related Report
      2009 Final Research Report
  • [Book] 調理科学実験2004

    • Author(s)
      朝倉富子, 久木野睦子
    • Publisher
      京大出版センター
    • Related Report
      2009 Final Research Report
  • [Remarks] ホームページ等

    • Related Report
      2009 Final Research Report
  • [Patent(Industrial Property Rights)] 阿部啓子, 中島健一朗, 朝倉富子, 三坂巧, 石井翔2007

    • Inventor(s)
      甘味を有するタンパク質
    • Filing Date
      2007
    • Related Report
      2009 Final Research Report

URL: 

Published: 2007-04-01   Modified: 2016-04-21  

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