Analysis of prion and prion related protein for the purpose of food safety

• Project/Area Number: 19300251
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Eating habits, studies on eating habits
• Research Institution: Nara Women's University
• Principal Investigator: MATSUDA Satoru Nara Women's University, 生活環境学部, 教授 (50242110)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000); Fiscal Year 2008: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000); Fiscal Year 2007: ¥15,470,000 (Direct Cost: ¥11,900,000、Indirect Cost: ¥3,570,000)
• Keywords: 健康と食生活 / 食の安全 / プリオン / ドッペル / RNAi / NESH / 細胞運動 / 細胞死 / がん細胞 / 食健康 / がん

Research Abstract

アポトーシスや細胞膜抗酸化作用との関連を追究するために、プリオン及びドッペルの発現ベクターを作製して、リンパ系細胞に強制発現させ、紫外線照射時や血清除去時のアポトーシスに対する細胞の挙動変化を解析した。プリオン関連蛋白質と微小RNAとの結合の有無を検討したが、特定のRNAとは結合していない。ドッペル蛋白質ではプリオン蛋白質と異なる機能が報告されており、Bcl2関連アポトーシスカスケードの中で機能していることが示唆された。アダプター蛋白質NESHとの相互作用を免疫沈降やウエスタン法で確認した。SOD活性については、WAT1を用いて測定し、ドッペル蛋白質がSOD活性を阻害していることが判明した。

Research Products

• [Journal Article] Eriguchi M Tumor growth suppression by adenovirus-mediated introduction of a cell growth suppressing gene tob in a pancreatic cancer model (2009)
  • Author(s): Yanagie H, Tanabe T, Sumimoto H, Sugiyama H, Matsuda S, Nonaka Y, Ogiwara N, Sasaki K, Tani K, Takamoto S, Takahashi H
  • Journal Title: Biomed Pharmacother 63 Pages: 275-286
  • Peer Reviewed
• [Journal Article] Tumor growth suppression by adenovirus-mediated introduction of a cell growth suppressing gene tob in a pancreatic cancer model. (2009)
  • Author(s): Yanagie H, Matsuda S, et. al
  • Journal Title: Biomed Pharmacother. 63 Pages: 275-286
  • Peer Reviewed
• [Journal Article] NESH expression switches to the adverse effect of imatinib mesylate (2008)
  • Author(s): Matsuda S, Ichigotani Y, Okumura N, Yoshida H, Kajiya Y, Kitagishi Y, Shirafuji N
  • Journal Title: Mol Oncol. 2 Pages: 16-19
  • Peer Reviewed
• [Journal Article] Insulin receptor substrate protein 53 (IRSp53) as a binding partner of antimetastasis molecule NESH a member of Abelson interactor protein family (2008)
  • Author(s): Matsuda S, Yokozaki S, Yoshida H, Kitagishi Y, Shirafuji N, Okumura N
  • Journal Title: Ann. Oncol. 19 Pages: 1356-1357
  • Peer Reviewed
• [Journal Article] NESH protein expression switches to the adverse effect of imatinib mesylate. (2008)
  • Author(s): Matsuda S
  • Journal Title: Mol Oncol. 2 Pages: 16-19
  • Peer Reviewed
• [Journal Article] Insulin receptor substrate protein 53 (IRSp53) as a binding partner of anti-metasatasis molecule NESH, a member of Abelson interactor prote in family. (2008)
  • Author(s): Matsuda S
  • Journal Title: Ann. Oncol. 19 Pages: 1356-1357
  • Peer Reviewed
• [Journal Article] NESH protein expression switches to the adverse effect of imatinib mesylate. (2008)
  • Author(s): Matsuda S
  • Journal Title: Mol. Oncol. (In press)
  • Peer Reviewed
• [Journal Article] Insulin receptor substrate protein 53(IRSp53)as a binding partner of anti-metasatasis molecule NESH, a member of Abelson interactor protein family. (2008)
  • Author(s): Matsuda S
  • Journal Title: Ann. Oncol. (In press)
  • Peer Reviewed
• [Presentation] Prion類似膜糖蛋白質Doppelの機能解析 (2007)
  • Author(s): 松田覚
  • Organizer: 日本家政学会第59回大会
  • Place of Presentation: 長良川国際会議場
  • Year and Date: 2007-05-12