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The study on functional food using oxidative stress marker, hydroxyoctadecadienoic acid and 7-hydroxycholesterol

Research Project

Project/Area Number 19300256
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Eating habits, studies on eating habits
Research InstitutionNational Institute of Advanced Industrial Science and Technology

Principal Investigator

YOSHIDA Yasukazu  National Institute of Advanced Industrial Science and Technology, 健康工学研究センター, 研究センター付 (90358333)

Co-Investigator(Kenkyū-buntansha) NIKI Etsuo  独立行政法人産業技術総合研究所, 健康工学研究センター, 顧問 (20011033)
AKAZAWA Yoko  独立行政法人産業技術総合研究所, 健康工学研究センター, 特別研究員 (50549897)
SAITO Yoshiro  独立行政法人産業技術総合研究所, ヒューマンストレスシグナル研究センター, 研究員 (70357060)
Project Period (FY) 2007 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥15,600,000 (Direct Cost: ¥12,000,000、Indirect Cost: ¥3,600,000)
Fiscal Year 2009: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2008: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2007: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Keywords機能性食品 / 酸化ストレスマーカー / ヒドロキシリノール酸 / ヒドロキシコレステロール / タンパク質酸化修飾 / 酸化DJ-1 / 酸化ストレス / コエンザイムQ / ビタミンE / 酸化修飾DJ-1 / トコフェロール / 脂質酸化物 / トコフェリルキノン / グルタチオン / コレステロール / リノール酸 / 抗酸化物質
Research Abstract

Lipid peroxidation products have received a great deal of attention for the partical application to clinical use as bio-markers. To assess the antioxidative role of functional foods and antioxidants, vitamin E and BO-653, in a mouse model and culture cell using lipid peroxidation and protein oxidation products. In the present study, we proposed that lipid peroxidation (tHODE and tOHCh) and protein oxidation are indeed good markers for the detection of progression of oxidative stress and effect of functional foods.

Report

(4 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • 2007 Annual Research Report
  • Research Products

    (15 results)

All 2009 2008 2007

All Journal Article (12 results) (of which Peer Reviewed: 12 results) Presentation (2 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Preparation and application of monoclonal antibodies against oxidized DJ-1. Significant elevation of oxidized DJ-1 in erythrocytes of early-stage Parkinson disease patients2009

    • Author(s)
      斎藤芳郎, 濱窪隆雄, 吉田康一, 小川陽子(以下省略10人), 二木鋭雄
    • Journal Title

      Nuroscience Letters vol463

      Pages: 1-5

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of cellular uptake and distribution of coenzyme Q10 and vitamin E in PC12 cells.2009

    • Author(s)
      斎藤芳郎, 福原明子, 西尾敬子, 早川三恵子, 小川陽子, 坂本Hirokazu, 藤井健志, 吉田康一, 二木鋭雄
    • Journal Title

      Journal of Nutritional Biochemistry vol.20

      Pages: 350-357

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Preparation and application of monoclonal antibodies against oxidized DJ-1. Significant elevation of oxidized DJ-1 in erythrocytes of early-stage Parkinson disease patients.2009

    • Author(s)
      斉藤芳郎、濱窪隆雄、吉田康一、小川陽子、二木鋭雄
    • Journal Title

      Nurosciencce Letter Vol.463

      Pages: 1-5

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of cellular uptake and distribution of coenzyme Q10 and vitamin E in PC12 cells.2009

    • Author(s)
      斉藤芳郎、小川陽子、吉田康一、二木鋭雄
    • Journal Title

      Journal of Nutritional Biochemistry Vol.20

      Pages: 350-357

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Gamma-tocopheryl quinine, not alpha-tocopheryl quinine, induces adaptive response through up-regulation of cellular glutathione and cysteine anailability via activation ATF4.2008

    • Author(s)
      小川陽子, 斎藤芳郎, 西尾敬子, 吉田康一, 芦田均, 二木鋭雄
    • Journal Title

      Free Radical Resarch vol.42

      Pages: 674-687

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Acceleration of lipid peroxidation inα-tocopherol transfer protein-knockout mice following the consumption of drinking water containing a radical initiator.2008

    • Author(s)
      吉田康一, 早川三恵子, Cynshi Osamu, Jishage Kou-ichi, 二木鋭雄
    • Journal Title

      Journal of Oleo Science vol.57

      Pages: 577-583

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] γ-Tocopheryl quinine, not α-tocopheryl quinine, induces adaptive response through up-regulation of cellular glutathione and cysteine availability via activation of ATF42008

    • Author(s)
      Ogawa Y., et al
    • Journal Title

      Free Radical Research 42

      Pages: 674-687

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Acceleration of lipid peroxidation in a-tocopherol transfer protein-knockout mice following the consumption of drinking water containing a radical Initiator2008

    • Author(s)
      Yoshida Y., et al
    • Journal Title

      Journal of Oleo Science 57

      Pages: 577-583

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Antioxidant effects of 2, 3-dihydro-5-hydroxy-4, 6-di-tert-butyl-2, 2-dipentylbenzofur an and a-tocopherol in hyperlipidemic mice as evaluated by hydroxyoctadecadienoic acid and 7-hydroxycholesterol.2007

    • Author(s)
      吉田康一, 早川三恵子, 伊藤奈々子, 羽渕洋子, 井上ルリ子, Zhi-Hua Chen, Jiaofei Cao, Cynshi Osamu, Jishage Kou-Ichi, 二木鋭雄
    • Journal Title

      Biochemical Pharmacology vol.74

      Pages: 1010-1019

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Cholesterol is more susceptible to oxidation than linolates in cultured cells under oxidative stress induced by selenium deficiebcy and free radicals.2007

    • Author(s)
      斎藤芳郎, 吉田康一, 二木鋭雄
    • Journal Title

      FEBS Letters vol.581

      Pages: 4349-4354

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Antioxidant effects of 2,3-dihydro-5-hydroxy-4,6-di-tert-butyl-2,2-dipentylbenzoran and α-tocopherol inhyperlipidemic mice as evaluated by hydroxyoctadecadienoic acid and 7-hydroxycholesterol2007

    • Author(s)
      Yoshida Y., et. al.
    • Journal Title

      Biochem.Pharmacol. 74

      Pages: 1010-1019

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cholesterol is more susceptible to oxidation than linoleates in cultured cells under oxidativestress induced by selenium deficiency and free radicals2007

    • Author(s)
      Saito Y, Yoshida Y, and Niki E
    • Journal Title

      FEBS Lett 581

      Pages: 4349-4354

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] 酸化DJ-1とパーキンソン病(1)抗体作成とELISA開発2009

    • Author(s)
      斎藤芳郎, 小川陽子, 吉田康一, 二木鋭雄
    • Organizer
      第62回日本酸化ストレス学会学術総会
    • Year and Date
      2009-06-11
    • Related Report
      2009 Final Research Report
  • [Presentation] 酸化DJ-1とパーキンソン病(2)臨床検体の測定2009

    • Author(s)
      小川陽子, 斎藤芳郎, 吉田康一, 二木鋭雄
    • Organizer
      第62回日本酸化ストレス学会学術総会
    • Year and Date
      2009-06-11
    • Related Report
      2009 Final Research Report
  • [Patent(Industrial Property Rights)] 含硫アミノ酸残基が酸化された酸化型ポリペプチドに対する抗体2009

    • Inventor(s)
      吉田康一, 斎藤芳郎, 小川陽子, 二木鋭雄, 七里元督, 浜窪隆雄, 望月康弘, 岩成宏子, 絹見直樹
    • Industrial Property Rights Holder
      独立行政法人産業技術総合研究所、東京大学、株式会社ペルセウスプロテオミクス
    • Industrial Property Number
      2009-028042
    • Filing Date
      2009-02-10
    • Related Report
      2009 Final Research Report

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Published: 2007-04-01   Modified: 2016-04-21  

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