| Budget Amount *help |
¥20,410,000 (Direct Cost: ¥15,700,000、Indirect Cost: ¥4,710,000)
Fiscal Year 2009: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2008: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
Fiscal Year 2007: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
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| Research Abstract |
To clarify individual roles of chromatin structure changing-related factors in higher eukaryotic cells, we generated systematically a number of chicken DT40 mutant cell lines, respectively, lacking each of appropriate genes. By analyses of these mutant cells, we obtained important results as follows. (1) CAF-1-dependent nucleosome assembly is essential in DNA replication and cell proliferation via its interaction with PCNA. (2) HDAC2 regulates indirectly gene expressions of IgM H- and ・L chains via opposite controls of gene expressions of EBF1, Pax5, Aiolos plus Ikaros, and E2A. (3) GCN5 and BCR signaling regulate cooperatively pre-mature B cell apoptosis through both increases of caspase-3 and caspase-8 activities and decreases of ICAD and IAP2.
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