Analysis of phosphorylation signaling pathways

• Project/Area Number: 19310129
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied genomics
• Research Institution: The University of Tokyo
• Principal Investigator: IHARA Sigeo The University of Tokyo, 先端科学技術研究センター, 特任教授 (30345136)
• Co-Investigator(Kenkyū-buntansha): 浜窪 隆雄 東京大学, 先端科学技術研究センター, 教授 (90198797) ; 南敬 (南 敬) 東京大学, 先端科学技術研究センター, 特任准教授 (00345141) ; 川村 猛 東京大学, 先端科学技術研究センター, 特任助教 (70306835) ; 大田 佳宏 東京大学, 先端科学技術研究センター, 特任研究員 (80436592)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000); Fiscal Year 2008: ¥9,490,000 (Direct Cost: ¥7,300,000、Indirect Cost: ¥2,190,000); Fiscal Year 2007: ¥9,100,000 (Direct Cost: ¥7,000,000、Indirect Cost: ¥2,100,000)
• Keywords: リン酸化 / 転写複合体 / シミュレーション / プロテオミクス / 膜タンパク質複合体

Research Abstract

ヒト臍帯静脈上皮細胞、および脂肪前駆細胞の転写調節の動きをDNAアレイチップや機能を阻害する手法を用いて解析し、細胞内で刺激応答に起因する内在性のリン酸化状態変化を網羅的にプロテオミクスの観点からとらえることを目標にした。マイクロアレイの時系列データをもとにリン酸化シグナルパスウェイを予測し、報告者の文献情報データも含めたプロテオミクスの方法から検証し、刺激応答のリン酸化ダイナミクスに関係する遺伝子および蛋白質を選定し、シグナル伝達における細胞の反応のダイナミクスの一端を明らかにした。

Research Products

• [Journal Article] PPARγ/ RXRα Heterodimer Targets Genes of Histone Modification Enzymes Setd8 and Regulates Adipogenesis through a Positive Feed-Back (2009)
  • Author(s): Ken-ichi Wakabayashi, Masashi Okamura, Shuichi Tsutsumi, Naoko Nishikawa, Toshiya Tanaka, Iori Sakakibara, Jun-ichi Kitakami, Sigeo Ihara, Yuichi Hashimoto, Takao Hamakubo, Tatsuhiko Kodama, Hiroyuki Aburatani, and Juro Sakai
  • Journal Title: Mechanism, Mol. Cell. Biol MCB. 01856-08v1(In press)
  • Peer Reviewed
• [Journal Article] Nuclear Inhibitor of Protein Phosphatase-1 (NIPP1) Directs Protein Phosphatase-1 (PP1) to Dephosphorylate the U2 Small Nuclear Ribonucleoprotein Particle (snRNP) Component, Spliceosome-associated Protein 155 (Sap155) (2008)
  • Author(s): Nobuhiro Tanuma, Sei-Eun Kim, Monique Beullens, Yao Tsubaki, Shinya Mitsuhashi, Miyuki Nomura, Takeshi Kawamura, Kyoichi Isono, Haruhiko Koseki, Masami Sato, Mathieu Bollen, Kunimi Kikuchi, and Hiroshi Shima
  • Journal Title: The Journal of Biological Chemistry 283 Pages: 35805-35814
  • Peer Reviewed
• [Journal Article] Structural dependence of robustness and load tolerance in scale-free networks (2007)
  • Author(s): Nobuhiko Oshida and Sigeo Ihara
  • Journal Title: arXiv:0710.2513 710.2513
• [Journal Article] Structural dependence of robustness and load tolerance in scale-free networks
  • Author(s): Nobuhiko Oshida and Sigeo Ihara
  • Journal Title: arXiv : 0710.2513
• [Journal Article] PPARγ/RXRα Heterodimer Targets Genes of Histone Modification Enzymes Setd8 and Regulates Adipogenesis through a Positive Feed-Back Mechanism
  • Author(s): Wakabayashi et al
  • Journal Title: Mol. Cell. Biol. (印刷中)
  • Peer Reviewed
• [Presentation] Multiple Snapshots Obtained Using Genomic Tiling Arrays Reveal the Dynamics of Transcription in Human Umbilical (2008)
  • Author(s): Tatsuhiko Kodama, Yoshihiro Ohta, Meng Xu, Takashi Minami, Youichiro Wada, Shu-ichi Tsutsumi, Shumpei Ishikawa, Takao Hamakubo, Hiroyuki Aburatani, Peter R Cook, and Sigeo Ihara
  • Organizer: Endothelial Cells
  • Place of Presentation: Sydney, Australia
• [Presentation] Traffic efficiency of scale-free networks in biological systems (2007)
  • Author(s): Nobuhiko Oshida, Jun-ichi Kitakami, and Sigeo Ihara
  • Organizer: Cold spring Harvar Meeting, The Conference on Engineering Principles in Bio
  • Place of Presentation: Cold spring Harvar,NY,US
  • Year and Date: 2007-12-03
• [Presentation] High-resolution targeted proteomics of endogenousnuclear receptor comprex using high-quality immunomagnetic beads (2007)
  • Author(s): Kenji Daigo, Takeshi Kawamura, Satoshi Katayose, Yoshihiro Ohta,Toshiya Tanaka, Sigeo Ihara, Tatsuhiko Kodama, Takao Hamakubo
  • Organizer: Cold Spring Harbor Laboratory Mechanisms of Eukaryotic Transcription meeting
  • Place of Presentation: Cold Spring Harbor, NY
  • Year and Date: 2007-08-31
• [Presentation] HIGH-RESOLUTION TARGETED PROTEOMICS OF ENDOGENOUS NUCLEAR RECEPTOR COMPLEX USING HIGH-QUALITY IMMUNOMAGNETIC BEADS (2007)
  • Author(s): Kenji Daigo, Takeshi Kawamura, Satoshi Katayose, Toshiya Tanaka, Yoshihiro Ohta Sieo Ihara
  • Organizer: Cold spring Harvar Meeting, Mecharlisms of eukaryotic transcription
  • Place of Presentation: Cold spring Harvar,NY,US
  • Year and Date: 2007-08-30
• [Presentation] Packet traffic analysis of interactome networks.The Conference on Interactome Networks (2007)
  • Author(s): Nobuhiko Oshida, Naoko S.Nishikawa, Yoshihiro Ohta, Rikako Suzuki and Sieo Ihara
  • Organizer: Welcome Trust/Coldspring Harvar Meeting Interactome Network
  • Place of Presentation: Welcome Trust, Hinxton,UK
  • Year and Date: 2007-08-03
• [Presentation] Packet traffic analysis of interactome networks (2007)
  • Author(s): Nobuhiko Oshida, Naoko S. Nishikawa, Yoshihiro Ohta, Rikako Suzuki, and Sigeo Ihara
  • Organizer: The Conference on Interactome Networks, Welcome Trust
  • Place of Presentation: UK
• [Presentation] Robustness analysis of network topology and node removal strategy (2007)
  • Author(s): Nobuhiko Oshida, and Sigeo Ihara
  • Organizer: European Conference on Complex Systems, Dresden
  • Place of Presentation: Germany
• [Presentation] Robustness of the protein interaction network (2007)
  • Author(s): Nobuhiko Oshida, Jun-ichi Kitakami, Naoko S. Nishikawa, Yoshihiro Ohta, and Sigeo Ihara
  • Organizer: The 7th international workshop on advanced genomics
  • Place of Presentation: Tokyo, Japan
• [Presentation] Robustness analysis of network topology and node removal strategy (2007)
  • Author(s): Nobuhiko Oshida and Sigeo Ihara
  • Organizer: European Conference on Complex Systems
  • Place of Presentation: Dresden,Germany
• [Presentation] Robustness of the protein interaction networks (2007)
  • Author(s): Nobuhiko Oshida, Jun-ichi Kitakami, Naoko S. Nishikawa, Yoshihiro Ohta, and Sieo Ihara
  • Organizer: The 7th international workshop on advanced genomics
  • Place of Presentation: Tokyo
• [Presentation] Traffic efficiency of scale-free networks in biological systems. The Conference on Engineering Principles in Biological Systems (2006)
  • Author(s): Nobuhiko Oshida, Jun-ichi Kitakami, and Sigeo Ihara
  • Organizer: Cold Spring Harbor Laboratory
  • Place of Presentation: USA
• [Remarks]
  • URL: http://www.lsbm.org/
• [Remarks]
  • URL: http://www.escut.org/
• [Remarks]
  • URL: http://www.lsbm.org/
• [Remarks]
  • URL: http://www.lsbm.org/