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Functional significance of enzymes involved in conjugation and de-conjugation of isoflavones in the growth and symbiosis of soybean plant

Research Project

Project/Area Number 19310134
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Living organism molecular science
Research InstitutionTohoku University

Principal Investigator

NAKAYAMA Toru  Tohoku University, 大学院・工学研究科, 教授 (80268523)

Co-Investigator(Kenkyū-buntansha) 高橋 征司  東北大学, 大学院・工学研究科, 助教 (90343061)
Project Period (FY) 2007 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
Fiscal Year 2008: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2007: ¥13,260,000 (Direct Cost: ¥10,200,000、Indirect Cost: ¥3,060,000)
Keywordsイソフラボン / ダイズ / グルコシダーゼ / グルコシルトランスフェラーゼ / 遺伝子発現 / 修飾・脱修飾 / 微生物感染 / 根粒菌 / マロニルトランスフェラーゼ / β-グルコシダーゼ / ファイトアレキシン / プロモーター / 相互作用
Research Abstract

本研究では, ダイズ根の根粒菌感染におけるイソフラボンの修飾・脱修飾酵素遺伝子(GmIF7GT, GmIF7MaT, GmICHG)および生合成酵素遺伝子(GmIFS1, GmIFS2)の発現レベルの変動を解析し, 植物-共生微生物間の相互作用における同酵素群の生理的役割について考察した. 解析の結果, 根粒菌感染による生合成・修飾・脱修飾いずれの酵素遺伝子の発現レベルも変動しないことが明らかとなった.

Report

(3 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • Research Products

    (23 results)

All 2009 2008 2007

All Journal Article (10 results) (of which Peer Reviewed: 10 results) Presentation (8 results) Book (2 results) Patent(Industrial Property Rights) (3 results)

  • [Journal Article] Kinetic and thermodynamic characterization of the cold activity acquired upon single amino-acid substitution near the active site of a thermostable α-glucosidase2008

    • Author(s)
      Akio Noguchi, Tokuzo Nishino, Toru Nakayama
    • Journal Title

      Journal of Molecular Catalysis B : Enzymatic 56

      Pages: 300-306

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] cDNA cloning of glycosyltransferases from Chinese wolfberry (Lycium barbarum L.) fruits and enzymatic synthesis of a catechin glucoside using a recombinant enzyme (UGT73A10)2008

    • Author(s)
      A. Noguchi, N. Sasaki, M. Nakaoa, H. Fukami, S. Takahashi, T. Nishino, T. Nakayama
    • Journal Title

      Journal of Molecular Catalysis B : Enzymatic 55

      Pages: 84-92

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The product chain length determination mechanism of type II geranylgeranyl diphosphate synthase requires subunit interaction2008

    • Author(s)
      M. Noike, T. Katagiri, T. Nakayama, T. Koyama, T. Nishino and H. Hemmi
    • Journal Title

      The FEBS Journal 275

      Pages: 3921-3933

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Anthocyanin acyltransferase engineered for the synthesis of a novel polyacylated anthocyanin2007

    • Author(s)
      Hirokazu Suzuki, Tokuzo Nishino, Toru Nakayama
    • Journal Title

      Plant Biotechnology 24

      Pages: 495-501

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Structural and Mutational Studies of Anthocyanin Malonyltransferases Establish the Features of BAHD Enzyme Catalysis2007

    • Author(s)
      Hideaki Unno, Fumiko Ichimaida, Hirokazu Suzuki, Seiji Takahashi, Yoshikazu Tanaka, Atsushi Saito, Tokuzo Nishino, Masami Kusunoki, and Toru Nakayama
    • Journal Title

      The Journal of Biological Chemistry 282

      Pages: 15812-158122

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] cDNA cloning of a BAHD acyltransferase from soybean(Glycine max) : isoflavone 7-O-glucoside-6'-O-malonyltransferase2007

    • Author(s)
      Hirokazu Suzuki, Tokuzo Nishino, Toru Nakayama
    • Journal Title

      Phytochemistry 68

      Pages: 2035-2042

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] A UDP-glucose : isoflavone 7-O-glucosyltransferase from the roots of soybean(Glycine max) seedlings : PURIFICATION, GENECLONING, PHYLOGENETICS, AND AN IMPLICASTION FOR AN ALTERNATIVE STRATEGY OF ENZYME CATALYSIS2007

    • Author(s)
      Akio Noguchi, Atsushi Saito, Yu Homma, Masahiro Nakao, Nobuhiro Sasaki, Tokuzo Nishino, Seiji Takahashi, and Toru Nakayama
    • Journal Title

      The Journal of Biological Chemistry 282

      Pages: 23581-23590

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] A UDP-glucose: isoflavone 7-O-Glucosyltransferase from the Roots of Soybean(Glycine max)Seedlings: PURIFICATION, GENECLONING,PHYLOGENETICS, AND AN IMPLICASTION FOR AN ALTERNATIVE STRATEGY OF ENZYME CATALYSIS2007

    • Author(s)
      Akio Noguchi, et. al.
    • Journal Title

      The Journal of Biological Chemistry 282

      Pages: 23581-23590

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] cDNA cloning of a BAHD acyltransferase from soybean(Glycinemax): Isoflavone 7-O-glucoside-6"-O-malonyltransferase2007

    • Author(s)
      Hirokazu Suzuki, et. al.
    • Journal Title

      Phytochemistry 68

      Pages: 2035-2042

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Structural and Mutational Studies of Anthocyanin Malonyltransferases Establish the Features of BAHD Enzyme Catalysis2007

    • Author(s)
      Hideaki Unno, et. al.
    • Journal Title

      The Journal of Biological Chemistry 282

      Pages: 15812-15822

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] 花色発現にかかわるフラボノイドの生合成の酵素科学2008

    • Author(s)
      中山亨
    • Organizer
      富山県立大学生物工学研究センター講演会
    • Place of Presentation
      富山県射水市
    • Year and Date
      2008-09-19
    • Related Report
      2008 Final Research Report
  • [Presentation] キンギョソウのフラボノイド代謝の細胞内区画化と代謝工学2008

    • Author(s)
      中山亨
    • Organizer
      第60回日本生物工学会大会シンポジウム「代謝と物質輸送から眺めた植物の科学と工学」
    • Place of Presentation
      仙台
    • Year and Date
      2008-08-28
    • Related Report
      2008 Final Research Report
  • [Presentation] キンギョソウのフラボノイド代謝の細胞内区画化と代謝工学2008

    • Author(s)
      中山亨
    • Organizer
      第60回日本生物工学会大会シンポジウム「代謝と物質輸送から眺めた植物の科学と工学」
    • Place of Presentation
      東北学院大学
    • Year and Date
      2008-08-28
    • Related Report
      2008 Annual Research Report
  • [Presentation] 花色発現の生化学と分子生物学2008

    • Author(s)
      中山亨
    • Organizer
      フロンティアバイオサイエンスコロキウム生命機能研究科第41回研究交流会
    • Place of Presentation
      大阪大学
    • Year and Date
      2008-07-09
    • Related Report
      2008 Annual Research Report
  • [Presentation] 花色発現の生化学と分子生物学2008

    • Author(s)
      中山亨
    • Organizer
      フロンティアバイオサイエンスコロキウム生命機能研究科第41回研究交流会
    • Place of Presentation
      大阪
    • Year and Date
      2008-07-08
    • Related Report
      2008 Final Research Report
  • [Presentation] Enzymology of aurone biosynthesis and its application in floriculture2008

    • Author(s)
      中山亨
    • Organizer
      4th Japan-Finland Biotechnology Symposium
    • Place of Presentation
      札幌市
    • Related Report
      2008 Final Research Report
  • [Presentation] 花色改変技術への期待: アントシアニンアシル基転移酵素の機能と構造2007

    • Author(s)
      中山 亨
    • Organizer
      平成19年度日本生物工学会大会
    • Place of Presentation
      広島大学
    • Year and Date
      2007-09-26
    • Related Report
      2007 Annual Research Report
  • [Presentation] Enzymolgy and molecular biology of aurone biosynthesis, The Third Korea-Japan Joint Symposium : Plant metabolism2007

    • Author(s)
      中山亨
    • Organizer
      biosynthesis to signal transduction
    • Place of Presentation
      Incheon, 韓国
    • Related Report
      2008 Final Research Report
  • [Book] Biosynthesis, Functions, and Applications2009

    • Author(s)
      Keiko Yonekura-Sakakibara, Toru Nakayama, Mami Yamazaki, Kazuki Saito
    • Publisher
      Anthocyanins(Springer)
    • Related Report
      2008 Final Research Report
  • [Book] Anthocyanins : Biosynthesis, functions, and applications2008

    • Author(s)
      Toru Nakayama, 他
    • Publisher
      Springer
    • Related Report
      2008 Annual Research Report
  • [Patent(Industrial Property Rights)] フラボノイド3位グルクロン酸転移酵素, 及びそれをコードするポリヌクレオチド2009

    • Inventor(s)
      中山亨, 小埜栄一郎, 福井祐子
    • Industrial Property Rights Holder
      サントリー
    • Industrial Property Number
      2009-011065
    • Filing Date
      2009-01-22
    • Related Report
      2008 Final Research Report
  • [Patent(Industrial Property Rights)] スーパーオキシドデイスムターゼを用いるアセトアルデヒド分解方法2008

    • Inventor(s)
      中山亨, 山口晴彦, 細矢美穂
    • Industrial Property Rights Holder
      東北大学サントリー
    • Industrial Property Number
      2008-284725
    • Filing Date
      2008-11-05
    • Related Report
      2008 Final Research Report
  • [Patent(Industrial Property Rights)] アセトアルデヒド分解微生物2008

    • Inventor(s)
      中山亨, 山口晴彦
    • Industrial Property Rights Holder
      東北大学サントリー
    • Industrial Property Number
      2008-202334
    • Filing Date
      2008-08-05
    • Related Report
      2008 Final Research Report

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Published: 2007-04-01   Modified: 2016-04-21  

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