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Development of a highly sensitive sensing technique using a nanoparticle hybrid array

Research Project

Project/Area Number 19350043
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Analytical chemistry
Research InstitutionOsaka Prefecture University

Principal Investigator

TSUTOMU Nagaoka  Osaka Prefecture University, 産学官連携機構, 教授 (00172510)

Co-Investigator(Kenkyū-buntansha) 椎木 弘  大阪府立大学, 産学官連携機構, 助教 (70335769)
Co-Investigator(Renkei-kenkyūsha) SHIIGI Hiroshi  大阪府立大学, 産学官連携機構, 准教授 (70335769)
Project Period (FY) 2007 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥14,040,000 (Direct Cost: ¥10,800,000、Indirect Cost: ¥3,240,000)
Fiscal Year 2008: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2007: ¥10,530,000 (Direct Cost: ¥8,100,000、Indirect Cost: ¥2,430,000)
Keywords金ナノ粒子 / DNA / 脱蛍光技術 / 電気抵抗検出 / ナノ粒子アレイ / 分子デバイス / 化学センサ / ナノ接合 / 電流検出 / アレイ / ナノ粒子配列膜 / SNP
Research Abstract

金ナノ粒子を1 nm程度の間隔で配列した2次元アレイを作製し, 電流検出型DNAセンサに応用した。50 nmの金ナノ粒子からなるアレイを作製し, その上にDNAを修飾した2 nmプローブ金ナノ粒子を配置した。このアレイで起こるDNAのハイブリッド化を電流測定により検討した。その結果, 50 fmolまでのDNA量の検出が可能であり, 1塩基ミスマッチも高選択的に検出することができた。

Report

(3 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • Research Products

    (19 results)

All 2009 2008 2007 Other

All Journal Article (9 results) (of which Peer Reviewed: 9 results) Presentation (3 results) Book (4 results) Remarks (3 results)

  • [Journal Article] Label-Free DNA Detection Based on Opening Bridge Structured Gold-Nanoparticle Array2008

    • Author(s)
      S. Tokonami, H. Shiigi, T. Nagaoka
    • Journal Title

      Anal. Chem 80

      Pages: 8071-8075

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Label-free DNA Detection of Nano-Gapped Gold Nanoparticle Array Electrode2008

    • Author(s)
      S. Tokonami, H. Shiigi, T. Nagaoka
    • Journal Title

      J. Electrochem. Soc 155

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Praparation of Nano-Gapped Gold Nanoparticle Array For DNA detection2008

    • Author(s)
      S. Tokonami, H. Shiigi, T. Nagaoka
    • Journal Title

      Electroanalysis 20

      Pages: 355-360

    • Related Report
      2008 Annual Research Report 2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Label-Free DNA Detection Based on Opening Bridge structured Gold-Nanoparticle Array2008

    • Author(s)
      S. Tokonami, H. Shiigi, T. Nagaoka
    • Journal Title

      Anal. Chem. 80

      Pages: 8071-8075

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Label-free DNA Detection of Nano-Gapped Gold Nanoparticle Array Electrode2008

    • Author(s)
      S. Tokonami, H. Shiigi, T. Nagaoka
    • Journal Title

      J. Electrochem. Soc. 155

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Label-free DNA detection of nano-gapped gold nanoparticle array electrode2008

    • Author(s)
      S. Tokonami
    • Journal Title

      J. Electrochem. Soc. 155

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Preparation of nano-gapped nanoparticle array for DNA detection2008

    • Author(s)
      S. Tokonami
    • Journal Title

      Electroanalysis 20

      Pages: 355-360

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 「金ナノ粒子を利用する増感化学センサー技術」2007

    • Author(s)
      長岡勉
    • Journal Title

      分析化学 56

      Pages: 201-212

    • NAID

      110006249641

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] 金ナノ粒子を利用する増感化学センサー技術2007

    • Author(s)
      長岡 勉
    • Journal Title

      分析化学 56

      Pages: 201-212

    • NAID

      110006249641

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] Electrical DNA detection with gold nanoparticle arrays2008

    • Author(s)
      T. Nagaoka
    • Organizer
      REMSEA 2008
    • Place of Presentation
      Singapore
    • Year and Date
      2008-08-05
    • Related Report
      2008 Annual Research Report 2008 Final Research Report
  • [Presentation] A nanoparticle-array detector based on optical and conductivity observation2007

    • Author(s)
      T. Nagaoka
    • Organizer
      14th International Conference on Flow Injection Analysis
    • Place of Presentation
      Berlin
    • Year and Date
      2007-09-06
    • Related Report
      2008 Final Research Report
  • [Presentation] A nanoparticle-array detector based on optical and conductivity observation2007

    • Author(s)
      T.Nagaoka
    • Organizer
      14th International Conference on Flow Injection Analysis
    • Place of Presentation
      Institute of Physics of the Technical University,Berhn,Germany
    • Year and Date
      2007-09-06
    • Related Report
      2007 Annual Research Report
  • [Book] S. Tokonami, H. Shiigi, T. Nagaoka, Gold Nano Films Synthesis, Characterization, and Potential Biomedicai Applications, Nanomaterials for the Life Sciences2009

    • Author(s)
      S. Tokonami, H. Shiigi, T. Nagaoka
    • Publisher
      Wiley-VCH(印刷中)
    • Related Report
      2008 Annual Research Report
  • [Book] 「産学官連携活動の実際第13章」2008

    • Author(s)
      長岡勉(分担執筆)
    • Publisher
      中央経済社
    • Related Report
      2008 Final Research Report
  • [Book] 産学官連携活動の実際2008

    • Author(s)
      長岡 勉
    • Publisher
      中央経済社
    • Related Report
      2007 Annual Research Report
  • [Book] " Gold Nano Films Synthesis, Characterization, and Potential Biomedical Applications," Nanomaterials for the Life Sciences,(Ed. Dr. Challa S. S. Rl. Kumar)

    • Author(s)
      S. Tokonami, H. Shiigi, T. Nagaoka
    • Publisher
      Wiley-VCH(in press)
    • Related Report
      2008 Final Research Report
  • [Remarks]

    • URL

      http://tokachi.riast.osakafu-u.ac.jp/%7Esentan1/home.html

    • Related Report
      2008 Final Research Report
  • [Remarks]

    • URL

      http://tokachi.riast.osakafu-u.ac.jp/~sentan1/home.html

    • Related Report
      2008 Annual Research Report
  • [Remarks]

    • URL

      http://tokachi.riast.osakafu-u.ac.jp/%7Esentan1/home.html

    • Related Report
      2007 Annual Research Report

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Published: 2007-04-01   Modified: 2016-04-21  

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