Developments of Fluorescent Probes for Elucidation of Biological Functions of Phosphate Species
Project/Area Number |
19350085
|
Research Category |
Grant-in-Aid for Scientific Research (B)
|
Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Chemistry related to living body
|
Research Institution | Kyoto University |
Principal Investigator |
OJIDA Akio Kyoto University, 大学院・工学研究科, 講師 (10343328)
|
Project Period (FY) |
2007 – 2009
|
Project Status |
Completed (Fiscal Year 2009)
|
Budget Amount *help |
¥14,560,000 (Direct Cost: ¥11,200,000、Indirect Cost: ¥3,360,000)
Fiscal Year 2009: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2008: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2007: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
|
Keywords | 蛍光プローブ / バイオセンサー / バイオイメージング / リン酸アニオン / タンパク質 / FRET / レシオ検出 / キナーゼ / 分子認識 / 蛋白質 |
Research Abstract |
In this research project, we have developed the new fluorescent probes, which are useful for elucidation of biological functions of various biological phosphate species. We especially focused on the developments of the three types of the fluorescent probes, including 1) protein-based hybrid-type biosensor for phosphorylated protein/peptide, 2) molecular probe for aggregation of phosphorylated tau protein exisiting in brain of Alztheimer's disease, and 3) molecular probe for intracellular ATP with a OFF/ON-type fluorescence sensing mode. As the results, we have successfully achieved the probes with the desirable functions in each of the three themes.
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Report
(4 results)
Research Products
(21 results)