Structural basis of functional changes of RNA modification enzymes through evolution of life

• Project/Area Number: 19350087
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Chemistry related to living body
• Research Institution: Ehime University
• Principal Investigator: HORI Hiroyuki Ehime University, 大学院・理工学研究科, 教授 (20256960)
• Co-Investigator(Kenkyū-buntansha): NUREKI Osamu 東京大学, 医科学研究所, 教授 (10272460) ; YOKOBORI Shin-ichi 東京薬科大学, 生命科学部, 講師 (40291702)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000); Fiscal Year 2009: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2008: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2007: ¥9,880,000 (Direct Cost: ¥7,600,000、Indirect Cost: ¥2,280,000)
• Keywords: 酵素化学 / 核酸 / 酵素 / 生体分子 / ゲノム / 進化

Research Abstract

Living organisms can be classified to three domains, eukaryotes, archaea and eubacteria. The purpose of this study is clarification of structural and functional differences of RNA modification enzymes according to the evolution of life. Our new findings are as follows : discovery of new multisite-specificity RNA methyltransferase, structural study and hypothetical reaction mechanism of folate-dependent RNA methyltransferase, multistep recognition of substrate tRNA by protein-knot RNA methyltransferase, and discovery of RNA modification network.

Research Products

• [Journal Article] Flexible recognition of the tRNA G18 methylation target site by TrmH methyltransferase through first binding and induced fit processes (2010)
  • Author(s): A. Ochi, K. Makabe, K. Kuwajima, H. Hori
  • Journal Title: J. Biol. Chem. 285(in press) Pages: 9018-9029
• [Journal Article] N7-Methylguanine at position 46 (m7G46) in tRNA from Thermus thermophilus is required for cell viability through a tRNA modification network. (2010)
  • Author(s): C. Tomikawa, T. Yokogawa, T. Kanai, H. Hori
  • Journal Title: Nucleic Acids Res. 38 Pages: 942-957
• [Journal Article] Flexible recognition of the tRNA G18 methylation target site by Trm H methyltransferase through first binding and induced fit processes (2010)
  • Author(s): A.Ochi, K.Makabe, K.Kuwajima, H.Hori
  • Journal Title: J.Biol.Chem. 285 Pages: 9018-9029
  • Peer Reviewed
• [Journal Article] N^7-Methylguanine at position 46(m^7G46)in tRNA from Thermus thermophilus is required for cell viability through a tRNA modification network (2010)
  • Author(s): C.Tomikawa, T.Yokogawa, T.Kanai, H.Hori
  • Journal Title: Nucleic Acids Res. 38 Pages: 942-957
  • Peer Reviewed
• [Journal Article] (2010)
  • Author(s): H.Hori
  • Journal Title: Methods in Mol. Biol. vol. 607(Hamana Press) Pages: 173-185
• [Journal Article] Aquifex aeolicus tRNA (N2, N2-guanine)-dimethyltransferase (Trm1) catalyzes transfer of methyl groups not only to guanine 26 but also to guanine 27 in tRNA. (2009)
  • Author(s): T. Awai, S. Kimura, C. Tomikawa, A. Ochi, Ihsanawati, Y. Bessho, S. Yokoyama, S. Ohno, K. Nishikawa, T. Yokogawa, T. Suzuki, H. Hori
  • Journal Title: J. Biol. Chem. 284 Pages: 20467-20478
• [Journal Article] Atomic structure of a folate/FAD-dependent tRNA T54 methyltransferase (2009)
  • Author(s): H. Nishimasu, R. Ishitani, K. Yamashita, C. Iwashita, A. Hirata, H. Hori, O. Nureki
  • Journal Title: Proc. Natl. Acad. Sci. USA 106 Pages: 8180-8185
• [Journal Article] Micro-NanoMechatronics and Human Science (2009)
  • Author(s): T. Kitajima, A. Hirata, C. Iwashita, S.-I. Yokobori H. Hori
  • Journal Title: CFP09768-CDR P2-13
• [Journal Article] Production of yeast (m2G10) methyltransferase (Trm11 and Trm112 complex) in a wheat germ cell-free translation system. (2009)
  • Author(s): K. Okada, Y. Muneyoshi, Y. Endo, H. Hori
  • Journal Title: Nucleic Acids Res. Symp. Ser. 53 Pages: 303-304
• [Journal Article] Aquifex aeolicus tRNA(N^2, N^2-guanine)-dimethyltransferase(Trm1)catalyzes transfer of methyl groups not only to guanine 26 but also to guanine 27 in tRNA (2009)
  • Author(s): T.Awai, S.Kimura, C.Tomikawa, A.Ochi, Ihsanawati, Y.Bessho, S.Yokoyama, S.Ohno, K.Nishikawa, T.Yokogawa, T.Suzuki, H.Hori
  • Journal Title: J.Biol.Chem. 284 Pages: 20467-20478
  • Peer Reviewed
• [Journal Article] Atomic structure of a folate/FAD-dependent tRNA T54 methyltransferase (2009)
  • Author(s): H.Nishimasu, R.Ishitani, K.Yamashita, C.Iwashita, A.Hirata, H.Hori, O.Nureki
  • Journal Title: Proc.Natl.Acad. Sci.USA 106 Pages: 8180-8185
  • Peer Reviewed
• [Journal Article] Aquifex aeolicus tRNA(N^2, N^2-guanine)-dimethyltransferase(Trm1)catalyzes transfer of methyl groups not only to guanine 26 but also to guanine 27 in tRNA (2009)
  • Author(s): T.Kitajima, A.Hirata, C.Iwashita, S.Yokobori, H.Hori
  • Journal Title: IEEE : Micro NanoMechatronics and Human Science 2009 CFP09768-CDR Pages: 2-13
  • Peer Reviewed
• [Journal Article] Production of yeast(m^2G10)methyltransferase(Trm11 and Trm112 complex)in a wheat germ cell-free translation system (2009)
  • Author(s): K.Okada, Y.Muneyoshi, Y.Endo., H.Hori
  • Journal Title: Nucleic Acids Res.Symp.Ser. 53 Pages: 303-304
  • Peer Reviewed
• [Journal Article] Atomic structure of a folate/FAD-dependent tRNA T54 methyltransferase (2009)
  • Author(s): H. Nishimasu, R. Ishitani, K. Yamashita, C. Iwashita, A. Hirata, H. Hori, O. Nureki
  • Journal Title: Proc. Natl. Acad. Sci. USA (In press)
  • Peer Reviewed
• [Journal Article] Stabilization of tRNA (m1G37) methyltransferase [TrmD] from Aquifex aeolicus by an intersubunit disulfide bond formation (2008)
  • Author(s): T. Toyooka, T. Awai, T. Kanai, T. Imanaka, H. Hori
  • Journal Title: Genes to Cells 13 Pages: 807-816
• [Journal Article] Production of Hetero Subunits RNA Modification Enzyme by Wheat Germ Cell-Free Translation System (2008)
  • Author(s): K. Matsumoto, C. Tomikawa, T. Toyooka, A. Ochi, Y. Takano, N. Takayanagi, M. Abe, Y. Endo, H. Hori
  • Journal Title: J. of Biotechnol. 133 Pages: 453-460
• [Journal Article] The C-terminal region of thermophilic tRNA (m7G46) methyltransferase (TrmB) stabilizes the dimer structure and enhances fidelity of methylation. (2008)
  • Author(s): C. Tomikawa, A. Ochi, H. Hori
  • Journal Title: Proteins: Structure, Function and Bioinformtics 71 Pages: 1400-1408
• [Journal Article] Broad substrate RNA specificity of Trm1 [tRNA (m2 2G26) methyltransferase] from Aquifex aeolicus (2008)
  • Author(s): T. Awai, H. Hori
  • Journal Title: Micro-NanoMechatronics and Human Science P1-10
• [Journal Article] Stabilization of tRNA (m^1G37) methyltransferase [TrmD] from Aquifex aeolicus by an intersubunit disulfide bond formation (2008)
  • Author(s): T. Toyooka, T. Awai, T. Kanai, T. Imanaka, H. Hori
  • Journal Title: Genes to Cells 13 Pages: 807-816
  • Peer Reviewed
• [Journal Article] Broad substrate RNA specificity of Trml [tRNA (m^2G26) methyltransferase] from Aquifex aeolicus (2008)
  • Author(s): T. Awai, H. Hori
  • Journal Title: IEEE : Micro-NanoMechatronics and Human Science 2008 CEP07768-CDR Pages: 1-10
  • Peer Reviewed
• [Journal Article] The C-terminal region of thermophilic tRNA(m^7G46)methyltransferase(TrmB)stabilizes the dimer structure and enhances fidelity of methylation (2008)
  • Author(s): C. Tomikawa, A. Ochi, and H. Hori
  • Journal Title: Proteins: Structure, Function and Bioinformtics 71 Pages: 1400-1408
  • Peer Reviewed
• [Journal Article] Production of Hetero Subunits RNA Modification Enzyme by Wheat GermCell-Free Translation System (2008)
  • Author(s): K. Matsumoto, C. Tomikawa, T. Toyooka, A. Ochi, Y. Takano, N. Takayanagi, M. Abe, Y. Endo, and H. Hori
  • Journal Title: J.of Biotechnol. 133 Pages: 453-460
  • Peer Reviewed
• [Journal Article] RNA recognition mechanism of eukaryote tRNA (m7G46) methyltransferase (Trm8-Trm82 complex) (2007)
  • Author(s): K. Matsumoto, T. Toyooka, C. Tomikawa, A. Ochi, Y. Takano, N. Takayanagi, Y. Endo, H. Hori
  • Journal Title: FEBS Lett. 581 Pages: 1599-1604
• [Journal Article] Detection of structural changes in a cofactor binding protein by using a wheat germ cell-free protein synthesis system coupled with unnatural amino acid probing (2007)
  • Author(s): M. Abe, S. Ohno, T. Yokogawa, T. Nakanishi, F. Arisaka, T. Hosoya, T. Hiramatsu, M. Suzuki, T. Ogasawara, T. Sawasaki, K. Nishikawa, M. Kitamura, H. Hori, Y. Endo
  • Journal Title: Proteins: Structure, Function and Bioinformatics 67 Pages: 643-652
• [Journal Article] Complex Formations between Artificial RNA-DNA Chimera Nucleic Acids and RNA Modification Enzymes (2007)
  • Author(s): A. Ochi, H. Hori
  • Journal Title: Micro-NanoMechatronics and Human Science Pages: 113-118
• [Journal Article] Differences in substrate selectivity of the new SPOUT superfamily of methyltransferases (2007)
  • Author(s): T. Toyooka, H. Hori
  • Journal Title: Nucleic Acids Res. Symp. Ser. 51 Pages: 445-446
• [Journal Article] Hydroxyl Radical Probing of tRNA (Gm18) methyltransferase [TrmH]-AdoMet-artificial tRNA ternary complex (2007)
  • Author(s): A. Ochi, H. Hori
  • Journal Title: Nucleic Acids Res. Symp. Ser. 51 Pages: 373-374
• [Journal Article] Hetero subunit interaction and RNA recognition of yeast tRNA (m7G46) methyltransferase synthesized in a wheat germ cell-free translation system (2007)
  • Author(s): Y. Muneyoshi, K. Matsumoto, C. Tomikawa, T. Toyooka, A. Ochi, T. Masaoka, Y. Endo, H. Hori
  • Journal Title: Nucleic Acids Res. Symp. Ser. 51 Pages: 359-360
• [Journal Article] Hetero subunit interaction and RNA recognition of yeast tRNA(m^7G46)methyltransferase synthesized in a wheat germ cell-free translation system (2007)
  • Author(s): Y. Muneyoshi, K. Matsumoto, C. Tomikawa, T. Toyooka, A. Ochi, T. Masaoka, Y. Endo, and H. Hori
  • Journal Title: Nucleic Scids Res.Symp.Ser 51 Pages: 359-360
  • Peer Reviewed
• [Journal Article] Hydroxyl Radical Probing of tRNA(Gm18)methyltransferase[TrmH]-AdoMet-artificial tRNA ternary complex (2007)
  • Author(s): A. Ochi, and H. Hori
  • Journal Title: Nucleic Scids Res.Symp.Ser 51 Pages: 373-374
  • Peer Reviewed
• [Journal Article] Differences in substrate selectivity of the new SPOUT superfamily of methyltransferases (2007)
  • Author(s): T. Toyooka, and H. Hori
  • Journal Title: Nucleic Scids Res.Symp.Ser 51 Pages: 445-446
  • Peer Reviewed
• [Journal Article] Complex Formations between Artificial RNA-DNA Chimera Nucleic Acids and RNA Modification Enzymes (2007)
  • Author(s): A. Ochi, and H. Hori
  • Journal Title: Micro-NanoMechatronics and Human Science 2007 CEP07768-CDR Pages: 113-118
  • Peer Reviewed
• [Presentation] The lack of tRNA m^7G46 modification in Thermus thermophilus causes hypo-modification of other modifications in tRNA and depresses protein synthesis at high temperatures (2010)
  • Author(s): 冨川手恵, 横川隆志, 金井保, 堀弘幸
  • Organizer: 第8回科研費特定領域「バイオ操作」公開シンポジウム
  • Place of Presentation: 福岡
  • Year and Date: 2010-03-11
• [Presentation] Transfer RNA Methyltransferases from a Hyperthermophilic Eubacterium, Aquifex aeolicus (2010)
  • Author(s): H. Hori, T. Awai, T. Toyooka, C. Tomikawa, H. Okamoto, H. Takeda, K. Watanabe, A. Ochi, A. Hirata, S. Kimura, Y. Ikeuchi, T. Yokogawa, T. Suzuki
  • Organizer: 23rd tRNA workshop Portugal 2010
  • Place of Presentation: Aveiro, Portugal
  • Year and Date: 2010-01-28
• [Presentation] Multi-site substrate recognition mechanism of Aquifex aeolicus Trm1 (2010)
  • Author(s): T. Awai, Ihsanawati, S Kimura, C. Tomikawa, A. Ochi, T. Yokogawa, T. Suzuki, Y. Bessho, S. Yokoyama, H. Hori
  • Organizer: 23rd tRNA workshop Portugal 2010
  • Place of Presentation: Aveiro, Portugal
  • Year and Date: 2010-01-28
• [Presentation] Flexible recognition of methylation target site, guannosine at position 18 in tRNA by tRNA (Gm18) methyltransferase (2010)
  • Author(s): A. Ochi, H. Hori
  • Organizer: 23rd tRNA workshop Portugal 2010
  • Place of Presentation: Aveiro, Portugal
  • Year and Date: 2010-01-28
• [Presentation] Characterization of TrmJ [Transfer RNA (Cm32/Um32) Methyltransferase] Ortholog (TK1970) from a Hyperthermophilic Archaeon Thermococcus kodakarensis (2010)
  • Author(s): A. Hirata, A. Ochi, C. Tomikawa, T. Kitajima, T. Kanai, H. Hori
  • Organizer: 23rd tRNA workshop Portugal 2010
  • Place of Presentation: Aveiro, Portugal
  • Year and Date: 2010-01-28
• [Presentation] Atomic structure of a folate/FAD-dependent tRNA T54 methyltransferase (2010)
  • Author(s): H. Nishimasu, R. Ishitani, K. Yamashita, C. Iwashita, A. Hirata, H. Hori, O. Nureki
  • Organizer: 23rd tRNA workshop Portugal 2010
  • Place of Presentation: Aveiro, Portugal
  • Year and Date: 2010-01-28
• [Presentation] Flexible recognition of methylation target site, guannosine at position 18 in tRNA by tRNA(Gm18)methyltransferase (2010)
  • Author(s): 越智杏奈, 堀弘幸
  • Organizer: 23rd tRNA workshop Portugal 2010
  • Place of Presentation: Aveiro, Portugal
  • Year and Date: 2010-01-28
• [Presentation] Characterization of TrmJ [Transfer RNA(Cm32/Um32)Methyltransferase] Ortholog(TK1970)from a Hyperthermophilic Archaeon Thermococcus kodakarensis (2010)
  • Author(s): A.Hirata, A.Ochi, C.Tomikawa, T.Kitajima, T.Kanai, H.Hori
  • Organizer: 23rd tRNA workshop Portugal 2010
  • Place of Presentation: Aveiro, Portugal
  • Year and Date: 2010-01-28
• [Presentation] 超好熱性真正細菌Aquifex aeolicus由来Trm1 [tRNA (m2 2G26) methyltransferase]のtRNA認識機構と構造の相関 (2009)
  • Author(s): 粟井貴子、Ihsanawati, 木村聡、冨川千恵、越智杏奈、横川隆志、鈴木勉、別所義隆、横山茂之、堀弘幸
  • Organizer: 第32回日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-12
• [Presentation] 葉酸依存性tRNA m5U54メチル化酵素TrmFOの基質認識メカニズム解明に向けて (2009)
  • Author(s): 山下光輝、西増弘志、石谷隆一郎、岩下知香子、平田章、濡木理、堀弘幸
  • Organizer: 第32回日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-12
• [Presentation] 無細胞翻訳系を用いた酵母由来tRNA (m2G10)メチル基転移酵素(Trm11-Trm112)の合成と解析 (2009)
  • Author(s): 岡田和樹、宗吉裕樹、遠藤弥重太、堀弘幸
  • Organizer: 第32回日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-12
• [Presentation] Thermus thermophilusにおけるtRNAシュードウリジン55合成酵素TruBが生育およびtRNA修飾に与える影響 (2009)
  • Author(s): 石田一雄、冨川千恵、岩下知香子、堀弘幸
  • Organizer: 第32回日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-12
• [Presentation] 超好熱性真正細菌 Aquifex aeolicus 由来Tm1[tRNA(m^2_2G26)methyltransferase] のtRNA認識機構と構造の相関 (2009)
  • Author(s): 粟井貴子, Ihsanawati, 木村聡, 冨川千恵, 越智杏奈, 横川隆志, 鈴木勉, 別所義隆, 横山茂之, 堀弘幸
  • Organizer: 第32回日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-12
• [Presentation] 葉酸依存性tRNA m^5U54 メチル化酵素TrmF0の基質認識メカニズム解明に向けて (2009)
  • Author(s): 山下光輝, 西増弘志, 石谷隆一郎, 岩下知香子, 平田章, 濡木理, 堀弘幸
  • Organizer: 第32回日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-12
• [Presentation] 無細胞翻訳系を用いた酵母由来tRNA(m^2G10)メチル基転移酵素(Trm11-Trm112)の合成と解析 (2009)
  • Author(s): 岡田和樹, 宗吉裕樹, 遠藤弥重太, 堀弘幸
  • Organizer: 第32回日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-12
• [Presentation] Thermus thermophilus におけるtRNAシュードウリジン55合成酵素TruBが生育およびtRNA修飾に与える影響 (2009)
  • Author(s): 石田一雄, 冨川千恵, 岩下知香子, 堀弘幸
  • Organizer: 第32回日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-12
• [Presentation] 小麦胚芽無細胞タンパク質合成系とプロテインスプライシング反応を用いたRNase CL2発現システムの構築 (2009)
  • Author(s): 山内貴博, 岡田和樹, 宗吉裕樹, 横川隆志, 山崎俊夫, 遠藤弥重太, 堀弘幸
  • Organizer: 第32回日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-11
• [Presentation] Enzymatic and Crystallographic Characterization of Archaeal tRNA splicing endonuclease (2009)
  • Author(s): 北嶋翼, 平田章, 岩下知加子, 横堀伸一, 堀弘幸
  • Organizer: International MicroNano mechatronics and Human Science 2009
  • Place of Presentation: Nagoya
  • Year and Date: 2009-11-11
• [Presentation] FAD/葉酸依存性tRNA m5U54メチル化酵素TrmFOの基質認識メカニズムの解明に向けて (2009)
  • Author(s): 山下光輝、西増弘志、石谷隆一郎、岩下知香子、平田章、濡木理、堀弘幸
  • Organizer: 2009年日本化学会西日本大会
  • Place of Presentation: 松山
  • Year and Date: 2009-11-07
• [Presentation] 無細胞翻訳系を用いた酵母由来tRNA (m2G10) methyltransferase (Trm11-Trm112)の合成と解析 (2009)
  • Author(s): 岡田和樹、宗吉裕樹、遠藤弥重太、堀弘幸
  • Organizer: 2009年日本化学会西日本大会
  • Place of Presentation: 松山
  • Year and Date: 2009-11-07
• [Presentation] FAD/葉酸依存性tRNAm^5U54 メチル化酵素 TrmF0の基質認識メカニズムの解明に向けて (2009)
  • Author(s): 山下光輝, 西増弘志, 石谷隆一郎, 岩下知香子, 平田章, 濡木理, 堀弘幸
  • Organizer: 2009年 日本化学会西日本大会
  • Place of Presentation: 松山
  • Year and Date: 2009-11-07
• [Presentation] 無細胞翻訳系を用いた酵母由来 tRNA(m^2G10)methyltransferase(Trm11-Trm112)の合成と解析 (2009)
  • Author(s): 岡田和樹, 宗吉裕樹, 遠藤弥重太, 堀弘幸
  • Organizer: 2009年 日本化学会西日本大会
  • Place of Presentation: 松山
  • Year and Date: 2009-11-07
• [Presentation] Production of yeast tRNA(m2G10)methyltransferase(Trm11 and Trm112)in a wheat germ cell-free translation system (2009)
  • Author(s): 岡田和樹, 宗吉裕樹, 遠藤弥重太, 堀弘幸
  • Organizer: 6th International Symposium on Nucleic Acids Chemistry 2009
  • Place of Presentation: Takayama
  • Year and Date: 2009-09-27
• [Presentation] Synthesis yeast tRNA (m2G10) methyltransferase (Trm11 and Trm112) in a wheat germ cell-free translation system. (2009)
  • Author(s): K. Okada, Y. Muneyoshi, Y. Endo, H. Hori
  • Organizer: International Protein Island Matsuyama Symposium, Matsuyama
  • Year and Date: 2009-09-25
• [Presentation] Synthesis yeast tRNA(m2G10)methyltransferase(Trm11 and Trm 112)in a wheat germ cell-free translation system (2009)
  • Author(s): 岡田和樹, 宗吉裕樹, 遠藤弥重太, 堀弘幸
  • Organizer: International Protein Island Matsuyama Symposium
  • Place of Presentation: Matsuyama
  • Year and Date: 2009-09-25
• [Presentation] Construction of an expression system of RNase CL2 by a wheat germ cell-free translation system coupled with protein splicing reaction (2009)
  • Author(s): 山内貴博, 岡田和樹, 宗吉裕樹, 横川隆志, 山崎俊夫, 遠藤弥重太, 堀弘幸
  • Organizer: International Protein Island Matsuyama Symposium
  • Place of Presentation: Matsuyama
  • Year and Date: 2009-09-25
• [Presentation] Thermus thermophilus由来dihidrouridine合成酵素(Dus)の機能解析 (2009)
  • Author(s): 吉田剛士、岩崎絵梨、粟井貴子、冨川千恵、平田章、堀弘幸
  • Organizer: 第8回高度好熱菌丸ごと一匹プロジェクト連携会議・理研・播磨SPring-8
  • Year and Date: 2009-08-22
• [Presentation] FAD/Folate依存tRNA m5U54メチル化酵素TrmFOの基質認識メカニズム解明に向けて (2009)
  • Author(s): 山下光輝、西増弘志、岩下知香子、石谷隆一郎、平田章、濡木理、堀弘幸
  • Organizer: 第8回高度好熱菌丸ごと一匹プロジェクト連携会議・理研・播磨SPring-8
  • Year and Date: 2009-08-22
• [Presentation] Thermus thermophilusのtRNA修飾酵素遺伝子破壊株の解析-tRNA m7G46メチル化酵素はネットワークの鍵酵素の一つである- (2009)
  • Author(s): 冨川千恵、横川隆志、金井保、堀弘幸
  • Organizer: 第8回高度好熱菌丸ごと一匹プロジェクト連携会議・理研・播磨SPring-8
  • Year and Date: 2009-08-22
• [Presentation] Thermus thermophilus 由来 dihidrouridine 合成酵素(Dus)の機能解析 (2009)
  • Author(s): 吉田剛士, 岩崎絵梨, 粟井貴子, 冨川千恵, 平田章, 堀弘幸
  • Organizer: 第8回高度好熱菌丸ごと-匹プロジェクト連携会議
  • Place of Presentation: 理研・播磨・SPring-8
  • Year and Date: 2009-08-22
• [Presentation] Thermus thermophilusのtRNA修飾酵素遺伝子破壊株の解析-tRNAm^7G-46 ナチル化酵素はネットワークの鍵酵素の一つである- (2009)
  • Author(s): 冨川手恵, 横川隆志, 金井保, 堀弘幸
  • Organizer: 第8回高度好熱菌丸ごと一匹プロジェクト連携会議
  • Place of Presentation: 理研・播磨・SPring-8
  • Year and Date: 2009-08-22
• [Presentation] FAD/Folate 依存 tRNA m^5U54 メチル化酵素 TrmF0の基質認識メカニズム解明に向けて (2009)
  • Author(s): 山下光輝, 西増弘志, 岩下知香子, 石谷隆一郎, 平田章, 濡木理, 堀弘幸
  • Organizer: 第8回高度好熱菌丸ごと一匹プロジェクト連携会議
  • Place of Presentation: 理研・播磨・SPring-8
  • Year and Date: 2009-08-22
• [Presentation] 葉酸/FAD依存性tRNA U54メチル化酵素TrmFOの構造と機能 (2009)
  • Author(s): 西増弘志、石谷隆一郎、山下光輝、岩下知加子、平田章、堀弘幸、濡木理
  • Organizer: 第11回日本RNA学会
  • Place of Presentation: 新潟
  • Year and Date: 2009-07-28
• [Presentation] マルチサイト特異性RNAメチル化酵素のtRNA認識機構 (2009)
  • Author(s): 粟井貴子、木村聡、冨川千恵、越智杏奈、Ihsanawati、別所義隆、横山茂之、横川隆志、鈴木勉、堀弘幸
  • Organizer: 第11回日本RNA学会
  • Place of Presentation: 新潟
  • Year and Date: 2009-07-28
• [Presentation] 葉酸/FAD依存性tRNA U54メチル化酵素TrmF0の構造と機能 (2009)
  • Author(s): 西増弘志, 石谷隆一郎, 山下光輝, 岩下知加子, 平田章, 堀弘幸, 濡木理
  • Organizer: 第11回日本RNA学会
  • Place of Presentation: 新潟
  • Year and Date: 2009-07-28
• [Presentation] 古細菌(αβ)2型RNAスプライシングエンドヌクレアーゼはどのように変則的部位にあるイントロンを切断できるのか (2009)
  • Author(s): 北嶋翼、平田章、岩下知加子、横堀伸一、堀弘幸
  • Organizer: 第11回日本RNA学会
  • Place of Presentation: 新潟
  • Year and Date: 2009-07-27
• [Presentation] Thermus thermophilus HB8 tRNA (Gm18) methyltransferase [TrmH]のtRNA G18認識メカニズム (2009)
  • Author(s): 越智杏奈、堀弘幸
  • Organizer: 第11回日本RNA学会
  • Place of Presentation: 新潟
  • Year and Date: 2009-07-27
• [Presentation] tRNAm7G46修飾の有無は他tRNA修飾酵素活性に影響を与える (2009)
  • Author(s): 冨川千恵、金井保、横川隆志、堀弘幸
  • Organizer: 第11回日本RNA学会
  • Place of Presentation: 新潟
  • Year and Date: 2009-07-27
• [Presentation] 古細菌(αβ)2型RNAスプライシングエンドヌクレアーゼはどのように変則的都位にあるイントロンを切断できるのか (2009)
  • Author(s): 北嶋翼, 平田章, 岩下知加子, 横堀伸一, 堀弘幸
  • Organizer: 第11回日本RNA学会
  • Place of Presentation: 新潟
  • Year and Date: 2009-07-27
• [Presentation] Thermus thermophilus HB8 tRNA(Gm18)methyltransferase [TrmH]の tRNA G18認織メカニズム (2009)
  • Author(s): 越智杏奈, 堀弘幸
  • Organizer: 第11回日本RNA学会
  • Place of Presentation: 新潟
  • Year and Date: 2009-07-27
• [Presentation] tRNAm^7G46修飾の有無は他tRNA修飾酵素活性に影響を与える (2009)
  • Author(s): 冨川千恵, 金井保, 横川隆志, 堀弘幸
  • Organizer: 第11回日本RNA学会
  • Place of Presentation: 新潟
  • Year and Date: 2009-07-27
• [Presentation] 始原菌 (古細菌) tRNAスプライシングエンドヌクレアーゼによる変則的イントロンの切断 (2009)
  • Author(s): 北嶋 翼, 平田 章, 岩下 知香子, 横堀 伸一, 渡邊 洋一堀 弘幸
  • Organizer: 第7回科研費特定領域「バイオ操作」公開シンポジウム
  • Place of Presentation: 仙台
  • Year and Date: 2009-03-06
• [Presentation] tRNA修飾に対する翻訳伸長因子EF-Tuの影響 (2008)
  • Author(s): 中村仁, 堀弘幸
  • Organizer: 第31回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-13
• [Presentation] 好熱菌由来tRNA (m7G46) methyltransferase [TrmB]の触媒メカニズムの解明に向けた機能解析 (2008)
  • Author(s): 冨川千恵, 堀弘幸
  • Organizer: 第31回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-13
• [Presentation] 酵母由来tRNA (m7G46)メチル基転移酵素[Trm8-Trm82]の解析及び、ウリ類炭疽病菌Trm8オーソログAPH1遺伝子の比較解析 (2008)
  • Author(s): 宗吉裕樹、松本啓介、高野義孝、高柳直幸、遠藤弥重太、堀弘幸
  • Organizer: 第31回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-13
• [Presentation] 無細胞翻訳系を用いた酵母由来ヘテロサブユニットtRNA修飾酵素の合成と解析 (2008)
  • Author(s): 岡田和樹、宗吉裕樹、遠藤弥重太、堀弘幸
  • Organizer: 第31回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-13
• [Presentation] 真正細菌Aquifex aeolicus由来Trm1 [tRNA (m2 2G26) methyltransferase]の構造と基質認識メカニズム (2008)
  • Author(s): 粟井貴子、Ihsanawati、木村聡、冨川千恵、越智杏奈、横川隆志、鈴木勉、横山茂之、別所義隆、堀弘幸
  • Organizer: 第31回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-13
• [Presentation] 超好熱性古細菌Aeropyrum pernixにおけるtRNAThr前駆体とRNA修飾の解析 (2008)
  • Author(s): 北嶋翼、岩下知香子、横堀伸一、堀弘幸
  • Organizer: 第31回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-13
• [Presentation] 葉酸依存性tRNA(U54)メチル化酵素[TrmFO]の試験管内アッセイ法の確立 (2008)
  • Author(s): 山下光輝、岩下知香子, 西増弘志, 濡木理, 堀弘幸
  • Organizer: 第31回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-13
• [Presentation] Thermus thermophilusにおけるtRNAシュードウリジン55合成酵素TruBの重要性 (2008)
  • Author(s): 石田一雄、冨川千恵、越智杏奈、横川隆志、岩下知香子、堀弘幸
  • Organizer: 第31回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-13
• [Presentation] tRNA (Gm18) methyltransferase [TrmH] TypeI型酵素とTypeII型酵素の比較 (2008)
  • Author(s): 越智杏奈、堀弘幸
  • Organizer: 第31回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-13
• [Presentation] tRNA (Gm18) methyItransferase [TrmH] TypeI型酵素とTypell型酵素の比較 (2008)
  • Author(s): 越智杏奈, 堀 弘幸
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会合同年会(BMB2008)
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-09
• [Presentation] Thermus thermophilusにおけるtRNAシュードウリジン55合成酵素TruBの重要性 (2008)
  • Author(s): 石田 一雄, 冨川 千恵, 越智 杏奈, 横川 隆志, 岩下 知香子, 堀 弘幸
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会合同年会(BMB2008)
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-09
• [Presentation] 葉酸依存性tRNA(U54)メチル化酵素[TrmFO]の試験管内アツセイ法の確立 (2008)
  • Author(s): 山下 光輝, 岩下 知香子, 西増 弘志, 濡木 理, 堀 弘幸
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会合同年会(BMB2008)
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-09
• [Presentation] 超好熱性古細菌Aeropyrum pernixにおけるtRNA^<Thr>前駆体とRNA修飾の解析 (2008)
  • Author(s): 北嶋 翼, 岩下 知香子, 横堀 伸一, 堀 弘幸
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会合同年会(BMB2008)
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-09
• [Presentation] 真正細菌Aquifex aeolicus由来 Trmi [tRNA (m^2_2 G26) methyltransferase] の構造と基質認識メカニズム (2008)
  • Author(s): 粟井 貴子, Ihsanawati, 木村聡, 冨川 千恵, 越智 杏奈, 横川 隆志, 鈴木 勉, 横山 茂之, 別所 義隆, 堀 弘幸
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会合同年会(BMB2008)
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-09
• [Presentation] 無細胞翻訳系を用いた酵母由来ヘテロサブユニットtRNA修飾酵素の合成と解析 (2008)
  • Author(s): 岡田 和樹, 宗吉 裕樹, 遠藤 弥重太, 堀 弘幸
  • Organizer: 第31回日本分子生物学会年会・第8 1回日本生化学会合同年会(BMB2008)
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-09
• [Presentation] 酵母由来tRNA (m^7G46)メチル基転移酵素[Trm8-Trm82]の解析及び、ウリ類炭疽病菌Trm8オーソログAPH1遺伝子の比較解析 (2008)
  • Author(s): 宗吉 裕樹, 松本 啓介, 高野 義孝, 高柳 直幸, 遠藤 弥重太, 堀 弘幸
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会合同年会(BMB2008)
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-09
• [Presentation] 好熱菌由来tRNA (m^7G46) methyltransferase [TrmB] の触媒メカニズムの解明に向けた機能解析 (2008)
  • Author(s): 冨川千恵 堀 弘幸
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会合同年会(BMB2008)
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-09
• [Presentation] tRNA修飾に対する翻訳伸長因子EF-Tuの影響 (2008)
  • Author(s): 中村 仁, 堀 弘幸
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会合同年会(BMB2008)
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-09
• [Presentation] Broad substrate RNA specificity of Trml [tRNA (m^2_2 G26)methyltransferase] from Aquifex aeolicus (2008)
  • Author(s): T. Awai, H. Hori
  • Organizer: 2008 International Symposium on Micro-NanoMe chatronics and Human Science
  • Place of Presentation: Nagoya
  • Year and Date: 2008-11-06
• [Presentation] Yeast tRNA (m^7G46)methyltransferase which contains two protein subunits (Trm8 and Trm82) synthesized in a wheat germ cell-free translation system (2008)
  • Author(s): Y. Muneyoshi, K. Matsumoto, C. Tomikawa, T. Toyooka, Anna Ochi, T. Masaoka, Y. Endo, H. Hori
  • Organizer: Protein Island Matsuyama International Symposium 2008
  • Place of Presentation: Matsuyama
  • Year and Date: 2008-09-27
• [Presentation] Yeast tRNA (m7G46) methyltransferase which contains two protein subunits (Trm8 and Trm82) synthesized in a wheat germ cell-free translation system. (2008)
  • Author(s): Y. Muneyoshi, K. Matsumoto, C. Tomikawa, T. Toyooka, Anna Ochi, T. Masaoka, Y. Endo, H. Hori
  • Organizer: Protein Island Matsuyama International Symposium 2008
  • Place of Presentation: Matsuyama
  • Year and Date: 2008-09-25
• [Presentation] tRNAシュードウリジン55合成酵素TruB遺伝子破壊株及び変異株の生育について (2008)
  • Author(s): 石田一雄, 冨川千恵, 岩下知香子, 堀弘幸
  • Organizer: 高度好熱菌丸ごと一匹プロジェクト第7回連携研究会、理研・播磨
  • Year and Date: 2008-09-13
• [Presentation] Thermus thremophilus由来tRNA U54メチル化酵素TrmFO in vitroアッセイ法の確立 (2008)
  • Author(s): 山下光輝, 岩下知香子, 西増弘志, 濡木理, 堀弘幸
  • Organizer: 高度好熱菌丸ごと一匹プロジェクト第7回連携研究会、理研・播磨
  • Year and Date: 2008-09-13
• [Presentation] Thermus thermophilus tRNA(m1A58) methyltransferase [TrmI]の基質認識機構 (2008)
  • Author(s): 美濃地真之, 牛尾なつみ, 堀弘幸
  • Organizer: 高度好熱菌丸ごと一匹プロジェクト第7回連携研究会、理研・播磨
  • Year and Date: 2008-09-13
• [Presentation] 超好熱性真正細菌Aquifex aeolicusの推定上のtrm1遺伝子産物の性質決定 (2008)
  • Author(s): 粟井貴子、木村聡、Ihsanawati、冨川千恵、越智杏奈、別所義隆、横山茂之、横川隆志、鈴木勉、堀弘幸
  • Organizer: 高度好熱菌丸ごと一匹プロジェクト第7回連携研究会、理研・播磨
  • Year and Date: 2008-09-13
• [Presentation] Effects of tRNA (m7G46) modification in an extreme thermophile, Thermus thermophilus (2008)
  • Author(s): Chie Tomikawa, Takashi Yokogawa, Hiroyuki Hori
  • Organizer: FEBS Meeting
  • Place of Presentation: Greek
  • Year and Date: 2008-09-13
• [Presentation] 超好熱性真正細菌Aquifex aeolicusの推定上のtrml遺伝子産物の性質決定 (2008)
  • Author(s): 粟井貴子, 木村 聡, Ihsanawati, 冨川千恵, 越智杏奈, 別所義隆, 横山茂之, 横川隆志, 鈴木勉, 堀弘幸
  • Organizer: 高度好熱菌丸ごと一匹プロジェクト第7回連携研究会
  • Place of Presentation: 理研・播磨(SPring-8)
  • Year and Date: 2008-09-13
• [Presentation] Therms thermophilus tRNA (m^1 A58) methyltransferase [Trml]の基質認識機構 (2008)
  • Author(s): 美濃地 真之, 牛尾 なつみ, 堀 弘幸
  • Organizer: 高度好熱菌丸ごと一匹プロジェクト第7回連携研究会
  • Place of Presentation: 理研・播磨(SPring-8)
  • Year and Date: 2008-09-13
• [Presentation] Thermus thremophilus由来tRNA U54 メチル化酵素TrmFO in vitro アツセイ法の確立 (2008)
  • Author(s): 山下 光輝, 岩下 知香子, 西増 弘志, 濡木 理, 堀 弘幸
  • Organizer: 高度好熱菌丸ごと一匹プロジェクト第7回連携研究会
  • Place of Presentation: 理研・播磨(SPring-8)
  • Year and Date: 2008-09-13
• [Presentation] tRNAシュードウリジン55合成酵素TruB遺伝子破壊株及び変異株の生育について (2008)
  • Author(s): 石田 一雄, 冨川 千恵, 岩下 知香子, 堀 弘幸
  • Organizer: 高度好熱菌丸ごと一匹プロジェクト第7回連携研究会
  • Place of Presentation: 理研・播磨(SPring-8)
  • Year and Date: 2008-09-13
• [Presentation] 超好熱菌Aquifex aeolicus由来tRNA (m1G37)メチル化酵素[TrmD]のサブユニット間ジスルフィド結合形成の役割 (2008)
  • Author(s): 豊岡峻、粟井貴子、金井保、今中忠行、堀弘幸
  • Organizer: 第10回日本RNA学会年会
  • Place of Presentation: 札幌
  • Year and Date: 2008-07-25
• [Presentation] 高度好熱菌Thermus thermophilusにおけるtRNA m7G46修飾の役割は一体何か? (2008)
  • Author(s): 冨川千恵、横川隆志、堀弘幸
  • Organizer: 第10回日本RNA学会年会
  • Place of Presentation: 札幌
  • Year and Date: 2008-07-25
• [Presentation] Thermus thermophilus HB8 tRNA (Gm18) methyltransferase [TrmH] C末端領域による基質認識メカニズム (2008)
  • Author(s): 越智杏奈、堀弘幸
  • Organizer: 第10回日本RNA学会年会
  • Place of Presentation: 札幌
  • Year and Date: 2008-07-25
• [Presentation] 真正細菌Aquifex aeolicus由来Trm1 [tRNA (m2 2G26) methyltransferase]はG26のみならずG27もメチル化する (2008)
  • Author(s): 粟井貴子、木村聡、Ihsanawati、冨川千恵、越智杏奈、別所義隆、横山茂之、横川隆志、鈴木勉、堀弘幸
  • Organizer: 第10回日本RNA学会年会
  • Place of Presentation: 札幌
  • Year and Date: 2008-07-25
• [Presentation] 真正細菌Aquifex aeolicus由来Trm1 [tRNA (m22G26) methyltransferase] はG26のみならずG27もメチル化する (2008)
  • Author(s): 粟井貴子, 木村 聡, Ihsanawati, 冨川千恵, 越智杏奈, 別所義隆, 横山茂之, 横川隆志, 鈴木 勉, 堀 弘幸
  • Organizer: 第10回日本RNA学会年会
  • Place of Presentation: 札幌
  • Year and Date: 2008-07-25
• [Presentation] 高度好熱菌Thermus thermophilusにおけるtRNA m7G46修飾の役割は一体何か? (2008)
  • Author(s): 冨川千恵, 横川隆志, 堀 弘幸
  • Organizer: 第1O回日本RNA学会年会
  • Place of Presentation: 札幌
  • Year and Date: 2008-07-23
• [Presentation] 超好熱菌Aquifex aeolicus由来tRNA (m1G37)メチル化酵素 [TrmD] のサブユニット間ジスルフィド結合形成の役割 (2008)
  • Author(s): 豊岡 峻, 粟井貴子, 金井 保, 今中忠行, 堀 弘幸
  • Organizer: 第1O回日本RNA学会年会
  • Place of Presentation: 札幌
  • Year and Date: 2008-07-23
• [Presentation] RNA転写後複合体ネットワークの試験管内再構成 (2008)
  • Author(s): 堀 弘幸
  • Organizer: 第6回科研費特定領域「バイオ操作」公開シンポジウム
  • Place of Presentation: 京都
  • Year and Date: 2008-06-13
• [Presentation] 翻訳伸長因子EF-TuとtRNA修飾酵素の相互作用 (2007)
  • Author(s): 中村仁, 堀弘幸
  • Organizer: 第30回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-14
• [Presentation] 好熱菌由来tRNA (m7G46) methyltransferase (TrmB)のコアドメインならびにc末端領域の機能解析 (2007)
  • Author(s): 冨川千恵堀弘幸
  • Organizer: 第30回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-14
• [Presentation] 超好熱性真正細菌Aquifex aeolicus由来Trm1 [tRNA (m2 2G26) methyltransferase]の基質RNA認識メカニズム (2007)
  • Author(s): 粟井貴子、堀弘幸
  • Organizer: 第30回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-14
• [Presentation] 超好熱性真正細菌Aquifex aeolicus由来Trml[tRNA(m^2_2G26)methyltransferase]の基質RNA認識メカニズム (2007)
  • Author(s): 粟井 貴子、堀 弘幸
  • Organizer: 第30回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-13
• [Presentation] Thermus thermophilus HB8のtRNA修飾酵素生産量の生育環境変化による影響 (2007)
  • Author(s): 岩下 知香子、堀 弘幸
  • Organizer: 第30回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-13
• [Presentation] tRNA・グアニン・トランスグリコシラーゼによるDNAの塩基の交換 (2007)
  • Author(s): 車田 光謙、堀 弘幸
  • Organizer: 第30回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-13
• [Presentation] 好熱菌由来tRNA(m^7G46)methyltransferase(TrmB)のコアドメインならびにC末端領域の機能解析 (2007)
  • Author(s): 冨川 千恵、堀 弘幸
  • Organizer: 第30回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-13
• [Presentation] Transfer RNA methylation in Thermus thermophilus (2007)
  • Author(s): H.Hori, C.Iwashita, A.Shinkai, C.Tomikawa, Y.Terui, C.Nakamoto, K.Watanabe, N.Shigi, T.Suzuki, Y.Endo, T.Oshima, and K.Watanabe
  • Organizer: tRNA workshop 2007
  • Place of Presentation: Uppsala,Sweden
  • Year and Date: 2007-10-02
• [Presentation] Chemical probing of tRNA-protein interface in TrmH-AdoMet-artificial tRNA ternary complex (2007)
  • Author(s): A.Ochi, and H.Hori
  • Organizer: tRNA workshop 2007
  • Place of Presentation: Uppsala,Sweden
  • Year and Date: 2007-10-02
• [Presentation] Functions of the C-terminal region of thermophilic tRNA(m^7G46)methyltransferase(TrmB) (2007)
  • Author(s): C.Tomikawa, and H.Hori
  • Organizer: tRNA workshop 2007
  • Place of Presentation: Uppsala,Sweden
  • Year and Date: 2007-10-02
• [Presentation] Substrate Recognition of Trml[tRNA(m^2_2G26)methyltransferase]from Aquifex aeolicus (2007)
  • Author(s): T.Awai, Ihsanawati, T.Toyooka, Y.Bessho, S.Yokoyama, and H.Hori
  • Organizer: tRNA workshop 2007
  • Place of Presentation: Uppsala,Sweden
  • Year and Date: 2007-10-02
• [Presentation] 翻訳伸長因子EF-TuとtRNA修飾酵素の相互作用解析系の構築 (2007)
  • Author(s): 中村 仁, 堀 弘幸
  • Organizer: 高度好熱菌丸ごと一匹プロジェクト第6回連携研究会
  • Place of Presentation: 兵庫
  • Year and Date: 2007-08-03
• [Presentation] 生育環境変化によるtRNA修飾酵素生産量の変動 (2007)
  • Author(s): 岩下 知香子, 堀 弘幸
  • Organizer: 高度好熱菌丸ごと一匹プロジェクト第6回連携研究会
  • Place of Presentation: 兵庫
  • Year and Date: 2007-08-03
• [Presentation] 生命ドメインの変遷に伴うtRNA(m^7G46)メチル化酵素の分子進化 (2007)
  • Author(s): 冨川 千恵、松本 啓介、豊岡 峻、越智 杏奈、高柳 直行、高野 義孝、遠藤 弥重太、堀 弘幸
  • Organizer: 第9回日本RNA学会年会
  • Place of Presentation: 名古屋
  • Year and Date: 2007-07-30
• [Presentation] SPOUTスーパーファミリーメチル化酵素の構造と機能の相関 (2007)
  • Author(s): 豊岡 峻、堀 弘幸
  • Organizer: 第9回日本RNA学会年会
  • Place of Presentation: 名古屋
  • Year and Date: 2007-07-29
• [Book] Synthesis of a Hetero Subunit RNA Modification Enzyme by the Wheat Germ Cell-Free Translation System (2010)
  • Author(s): H. Hori
  • Publisher: Methods in Mol. Biol.
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