| Budget Amount *help |
¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000)
Fiscal Year 2009: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2008: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
Fiscal Year 2007: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
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| Research Abstract |
Nanoporous membranes with different pore dimensions were prepared from a series of polyethylene (PE)/polystyrene (PS) diblock copolymer films by acid etching under various conditions. Scanning probe microscope (SPM) observation indicated that the pore morphology was emphasized by elongation of etching time with elimination of amorphous PS phase. However, further etching destructs the PE crystalline phase which supports the network geometry of nanoporous membrane. For quantitative analyses of pore morphology, pore size distribution was evaluable by the height profiles extracted from a series of the scanning probe microscope images. The pore size was controllable by acid treatment conditions. Their biological applications were examined by cell proliferation tests using Hep G2 cells. Cell proliferation was confirmed for all membranes prepared in this study. However, membranes exhibiting typical nanoporous morphologies gave the lower numbers of cells after cell removal treatment by trypsin addition and centrifugation, compared to those for un-etched membrane having smooth surface. These results indicate the robust attachment between cell and membrane surface for the former membrane.
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