Nonlinear Raman microscopy to detect membrane protein
| Project/Area Number |
19360033
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied optics/Quantum optical engineering
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| Research Institution | Osaka University |
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Principal Investigator |
HASHIMOTO Mamoru Osaka University, 大学院・基礎工学研究科, 准教授 (70237949)
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| Co-Investigator(Kenkyū-buntansha) |
FUKUSHIMA Shuichiro 大阪大学, 大学院・基礎工学研究科, 助教 (40362644)
FUJITA Katsumasa 大阪大学, 大学院・工学研究科, 准教授 (80362664)
YOSHIKI Keisuke 兵庫県立大学, 大学院・工学研究科, 助教 (60432548)
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| Project Period (FY) |
2007 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000)
Fiscal Year 2009: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2008: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
Fiscal Year 2007: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
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| Keywords | ラマン散乱顕微鏡 / 非線形光学効果 / 振動分光学 / 膜タンパク質 / 光学顕微鏡 / 非線形光学 |
|---|
| Research Abstract |
We developed a multi-focus excitation nonlinear Raman microscope using a microlens array scanner for real-time molecular imaging. Parallel exposure of a specimen with light from two highly controlled ps mode-locked lasers (jitter of 30fs, point-by-point wavelength scan within 300ms) and parallel detection with an image sensor enabled real-time imaging. We demonstrated real-time Raman imaging of living cells (frame rate of 10fps). We also combined the developed system with the laser ablation to destruct the cell membrane, and demonstrated to observe the membrane repairing process. We added the polarization pattern control system to a nonlinear Raman microscopy system to detect membrane proteins by control the direction of electro-magnetic filed on the focus spot. The possibility of the selective detection of ordered molecules was confirmed with the imaging of liquid crystal.
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Report
(4 results)
Research Products
(51 results)
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[Journal Article]2009
Author(s)
井上康志, 川田善正, 渡慶次学, 橋本守, 火原彰秀
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Journal Title
顕微分光法ナノ・マイクロの世界を見る分光法(講談社サイエンティフィック)
Pages: 55-76
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