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Novel concept for creating molecul ar assembly-assisted extraction

Research Project

Project/Area Number 19360354
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Properties in chemical engineering process/Transfer operation/Unit operation
Research InstitutionKyushu University

Principal Investigator

GOTO Masahiro  Kyushu University, 工学研究院, 教授 (10211921)

Co-Investigator(Kenkyū-buntansha) KAMIYA Noriho  九州大学, 工学研究院, 准教授 (50302766)
KUBOTA Fukiko  九州大学, 工学研究院, 助教 (60294899)
丸山 達生  神戸大学, 大学院・工学研究科, 准教授 (30346811)
Co-Investigator(Renkei-kenkyūsha) MARUYAMA T Atuo  神戸大学, 工学, 准教授 (30346811)
Project Period (FY) 2007 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
Fiscal Year 2008: ¥8,840,000 (Direct Cost: ¥6,800,000、Indirect Cost: ¥2,040,000)
Fiscal Year 2007: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000)
Keywords逆ミセル / 分子集合体 / タンパク質抽出 / DNA / 溶媒抽出 / 逆キセル / マイクロエマルション / 抽出 / 分離 / タンパク質分離 / イオン液体 / 分子認識 / 抽出分離 / バイオ分子 / タンパク質 / 界面活性剤
Research Abstract

我々は、これまで金属イオンをはじめアミノ酸や核酸さらにはタンパク質などの抽出分離に関する研究を展開してきた。その結果, 単一の分子では抽出能力がない分子(主に界面活性分子)を, 特定の配列で組織化させることによって, 新たな抽出能力が発現することを見いだした。本研究では, これまで抽出という単位操作において使用されてきた単一の抽出試薬とは異なり, 認識ターゲットに対して高い抽出能および選択性を有するような分子集合試薬(複数の分子が集合してはじめて抽出能力を発揮するような分離素子)を開発した。その有用性をDNAの選択的抽出をモデルとして検証した。

Report

(3 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • Research Products

    (21 results)

All 2009 2008 2007

All Journal Article (16 results) (of which Peer Reviewed: 16 results) Presentation (5 results)

  • [Journal Article] Biocatalysis in Water-in-Ionic Liquid Microemulsions : A Case Study with Horseradish Peroxidase2009

    • Author(s)
      M.M. Zaman, N. Kamiya, and M. Goto
    • Journal Title

      Langmuir 25

      Pages: 977-982

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Biocatalysis in Water-in-Ionic Liquid Microemulsions : A Case Study with Horseradish Peroxidase2009

    • Author(s)
      M. M. Zaman, N. Kamiya, M. Goto
    • Journal Title

      Langmuir 25

      Pages: 977-982

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Surface modification of a solid-state cellulose matrix with lactose by a surfactant-enveloped enzyme in a nonaqueous medium2009

    • Author(s)
      S. Egusa, T. Kitaoka, M. Goto
    • Journal Title

      J. Mater. Chem. 19

      Pages: 1836-1842

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Design and in vivo evaluation of solid-in-oil suspension for oral delivery of human growth hormone2008

    • Author(s)
      H. Yoshiura, Y. Tahara, M. Hashida, N. Kamiya, A. Hirata, T. Fujii and M. Goto
    • Journal Title

      Biochemical Engineering Journal 41

      Pages: 106-110

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] A Nobel Solid-in-oil Nanosuspension for Transdermal Delivery of Diclofenac Sodium2008

    • Author(s)
      H. Piao, N. Kamiya, A. Hirata, T. Fujii and M. Goto
    • Journal Title

      Pharmaceutical Research 25

      Pages: 896-901

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Directed aggregation and fusion of lipid vesicles induced by DNA-surfactants2008

    • Author(s)
      T. Maruyama, H. Yamamura, M. Hiraki, Y. Kemori, H. Takata and M. Goto
    • Journal Title

      Colloids and Surfaces B : Biointerfaces 66

      Pages: 119-124

    • NAID

      120001623939

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] A solid-in-oil nanodispersion for transcutaneous protein delivery2008

    • Author(s)
      Y. Tahara, S. Honda, N. Kamiya, H. Piao, A. Hirata, E. Hayakawa, T. Fujii, M. Goto
    • Journal Title

      J. Control. Release 131

      Pages: 14-18

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Directed aggregation and fusion of lipid vesicles induced by DNA-surfactants2008

    • Author(s)
      T. Maruyama, M. Goto, et. al.
    • Journal Title

      Colloids and Surfaces B : Biointerfaces 66

      Pages: 119-124

    • NAID

      120001623939

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] An enzymatic method for site-specific labeling of recombinant proteins with oligonucleotides2007

    • Author(s)
      J. Tominaga, Y. Kemori, Y. Tanaka, T. Maruyama, N. Kamiya and M. Goto
    • Journal Title

      Chem. Commun

      Pages: 401-403

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Perfluorocarbon-based liquid-liquid extraction for separation of transition metal ions.,2007

    • Author(s)
      T. Maruyama, K. Nakashima, F. Kubota, M. Goto
    • Journal Title

      Anal. Sci. 23

      Pages: 763-765

    • NAID

      10019525982

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Calixarene-Assisted Protein Refolding via Liquid-Liquid Extraction2007

    • Author(s)
      K. Shimojo, T. Oshima, H. Naganawa, M. Goto
    • Journal Title

      Biomacromolecules 8

      Pages: 3061-3066

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Synthesis of Cellulose In Vitro by Using a Cellulase/Surfactant Complex in a Nonaqueous Mediumc2007

    • Author(s)
      S. Egusa, T. Kitaoka, M. Goto, H. Wariishi
    • Journal Title

      Angew. Chem. Int. Edit. 46(12)

      Pages: 2063-2065

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Sequence-selective extraction of single-stranded DNA using DNA-functionalized reverse micelles2007

    • Author(s)
      T. Maruyama, T. Hosogi, M. Goto
    • Journal Title

      Chem. Commun

      Pages: 4450-4454

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Sequence-selective extraction of single-stranded DNA using DNA-functionalized reverse micelles2007

    • Author(s)
      T. Maruyama, T. Hosogi, M. Goto
    • Journal Title

      Chemical Communications 2007

      Pages: 4450-2252

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Recognition of Lysine Residues on Protein Surfaces Using Calixarenes and Its Application2007

    • Author(s)
      T. Oshima, M. Goto, ら
    • Journal Title

      Current Drug Discovery Tech. 4

      Pages: 220-228

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Calixarene-Assisted Protein Refolding via Liquid-Liquid Extraction2007

    • Author(s)
      K. Shimojo, M. Goto, ら
    • Journal Title

      Biomacromolecules 8

      Pages: 3061-3066

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] DNA-酵素複合体の活性制御を利用した分析システムの開発2009

    • Author(s)
      嶋田如水、丸山達生、神谷典穂、後藤雅宏
    • Organizer
      化学工学会第74回年会
    • Related Report
      2008 Final Research Report
  • [Presentation] Sequence selective extraction of DNA using a molecular assembly2008

    • Author(s)
      M. Goto
    • Organizer
      AIChE annual meeting
    • Place of Presentation
      フィラデルフィア(米国)
    • Year and Date
      2008-11-18
    • Related Report
      2008 Annual Research Report
  • [Presentation] Sequence-Selective Extraction of DNA Using DNA Functionalized Reversed Micelles Engineering Conferences International2008

    • Author(s)
      M. Goto
    • Organizer
      Jan. at Costarica(招待講演)
    • Related Report
      2008 Final Research Report
  • [Presentation] イオン液体を用いた生体分子の抽出2007

    • Author(s)
      小柳 裕介、後藤 雅宏, ら
    • Organizer
      第44回化学関連支部合同九州大会
    • Place of Presentation
      北九州国際会議場
    • Related Report
      2007 Annual Research Report
  • [Presentation] Protein Transfer From Aqueous Phase to Ionic Liquids and Its Change of Biological Function2007

    • Author(s)
      M. Goto
    • Organizer
      The 2^<nd> Congress on Liquid Ions, 2007 Aug
    • Place of Presentation
      パシフィコ横浜
    • Related Report
      2007 Annual Research Report

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Published: 2007-04-01   Modified: 2016-04-21  

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