Feasibility of implantable liver tissues having a vascular-like blood flow network

• Project/Area Number: 19360371
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biofunction/Bioprocess
• Research Institution: The University of Tokyo
• Principal Investigator: SAKAI Yasuyuki The University of Tokyo, 生産技術研究所, 教授 (00235128)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥16,380,000 (Direct Cost: ¥12,600,000、Indirect Cost: ¥3,780,000); Fiscal Year 2009: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2008: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2007: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
• Keywords: 肝組織工学 / 胎児肝細胞 / 移植 / 三次元担体 / 液性因子 / 酸素供給 / 血流導入 / 生物・生体工学 / ES細胞

Research Abstract

Combination of 3D culture and soluble factors was very effective in maturation of fetal rat hepatocytes in vitro. Oxygen supply at appropriate exposure concentrations was also important to fully utilize the self-organization capacity of such hepatocyte progenitor population. Control of blood cell-to-implanted hepatocytes was shown to be a crucial issue in designing and engineering of implantable pre-vascularized liver tissues.

Research Products

• [Journal Article] Toward engineering of vascularized three-dimensional liver tissue equivalents possessing a clinicallysignificant mass (2010)
  • Author(s): Y. Sakai, H. Huang, S. Hanada, T. Niino
  • Journal Title: Biochem. Eng.J. 48 Pages: 348-361
  • Peer Reviewed
• [Journal Article] Toward engineering of vascularized three-dimensional liver tissue equivalents possessing a clinically-significant mass (2010)
  • Author(s): 酒井康行, H.Huang, 勝田毅, T.Niino
  • Journal Title: Biochemical Engineering Journal 48 Pages: 348-361
  • Peer Reviewed
• [Journal Article] 異所性体内埋め込み型人工肝臓開発の現状と展望 (2010)
  • Author(s): 勝田毅, 小森喜久夫, 寺谷工, 落谷孝広, 酒井康行
  • Journal Title: 生産研究(掲載確定) 62(印刷中)
  • NAID: 130000342799
• [Journal Article] Direct oxygen supply with polydimethylsiloxane(PDMS)membranes induces a spontaneous organization of thick heterogeneous liver tissues from rat fetal liver cells in vitro (2010)
  • Author(s): M.Hamon, 勝田毅, T.Fujii, 酒井康行
  • Journal Title: Cell Transplantation ((掲載確定), inpress.)
  • Peer Reviewed
• [Journal Article] 胚性幹細胞を用いた肝細胞分化誘導研究の現状 (2009)
  • Author(s): 勝田毅, 小森喜久夫, 酒井康行
  • Journal Title: 生産研究 61(2) Pages: 116-121
  • NAID: 130000102287
• [Journal Article] 胚性幹細胞を用いた肝細胞分化誘導研究の現状 (2009)
  • Author(s): 勝田毅, 小森喜久夫, 酒井康行
  • Journal Title: 生産研究 61 Pages: 116-121
  • NAID: 130000102287
• [Journal Article] Soluble factor-dependent in vitro growth and maturation of rat fetal liver cells in a three-dimensional culture system, T (2008)
  • Author(s): S. Hanada, N. Kojima, Y. Sakai
  • Journal Title: Tissue Eng. 14(1) Pages: 149-160
  • Peer Reviewed
• [Journal Article] A method for the design of 3D scaffolds for high-density cell attachment and determination of optimum perfusion culture conditions (2008)
  • Author(s): C. Provin, K. Takano, Y. Sakai, T. Fujii R. Shirakashi
  • Journal Title: J.Biomech. 41(7) Pages: 1436-1449
  • Peer Reviewed
• [Journal Article] 組織工学的手法を用いた肝組織再構築に関する研究の現状と課題 (2008)
  • Author(s): 花田三四郎, 酒井康行
  • Journal Title: 生産研究 60 Pages: 147-151
  • NAID: 110006936136
• [Journal Article] Soluble factor-dependent in vitro growth and maturation of rat fetal liver cells in a three-dimensional culture system (2008)
  • Author(s): S.Hanada, N.Kojima, Y.Sakai
  • Journal Title: Tissue Engineering Part A 14 Pages: 149-160
  • Peer Reviewed
• [Journal Article] 組織工学的手法を用いた肝組織再構築に関する研究の現状と課題 (2008)
  • Author(s): 花田 三四郎・酒井 康行
  • Journal Title: 生産研究 60 Pages: 147-151
  • NAID: 110006936136
• [Journal Article] Direct oxygen supply with poly dimethylsiloxane (PDMS) membranes induces a spontaneous organization of thick heterogeneous liver tissues from rat fetal liver cells in vitro
  • Author(s): M. Hamon, S. Hanada, T. Fujii, Y. Sakai
  • Journal Title: Cell Transplant (in press)
  • Peer Reviewed
• [Journal Article] Transplantation of a fetal liver cell-loaded hyaluronic acid sponge onto the mesentery recovers a Wilson's disease model rat
  • Author(s): T. Katsuda, T. Teratani, T. Ochiya, Y. Sakai
  • Journal Title: J.Biochem (in press)
  • NAID: 10027878100
  • Peer Reviewed
• [Presentation] 高代謝形臓器再構築のための担体デザインと三次元造形(招待講演) (2010)
  • Author(s): 酒井康行, 新野俊樹
  • Organizer: 機械学会第22回バイオエンジニアリング講演会
  • Place of Presentation: 岡山
  • Year and Date: 2010-01-10
• [Presentation] マウスES細胞からの効率的な肝細胞分化を目指した培養環境制御の導入 (2009)
  • Author(s): 勝田毅, 寺谷工, 落谷孝広, 酒井康行
  • Organizer: 第16回肝細胞研究会
  • Place of Presentation: 山形(口頭発表)
  • Year and Date: 2009-06-15
• [Presentation] 酸素分圧の段階的制御によるマウスES細胞からの肝細胞分化誘導 (2009)
  • Author(s): 勝田毅, 寺谷工, 落谷孝広, 酒井康行
  • Organizer: 第8回日本再生医療学会総会
  • Place of Presentation: 東京(口頭発表)
  • Year and Date: 2009-03-24
• [Presentation] 各種スケールの2D・3D組織再構築と工学的方法論, 招待講演 (2009)
  • Author(s): 酒井康行, 山口哲志, 小森喜久夫, 新海政重, 新野俊樹, 藤井輝夫, 長棟輝行, 牛田多加志
  • Organizer: 第8回日本再生医療学会総会
  • Place of Presentation: 東京
  • Year and Date: 2009-03-24
• [Presentation] 酸素分圧の段階的制御によるマウスES細胞からの肝細胞分化誘導 (2009)
  • Author(s): 勝田毅, 寺谷工, 落谷孝広, 酒井康行
  • Organizer: 第8回日本再生医療学会総会
  • Place of Presentation: 東京
  • Year and Date: 2009-03-05
• [Presentation] Fetal rat liver cell cultures : maintenance of liver function and formation of thick tissue induced by the enhancement of oxygen supply (2008)
  • Author(s): M Hamon, T Fujii, Y. Sakai
  • Organizer: Tissue Engineering and Regenerative Medicine International Society, Asia-Pacific Chapter Meeting 2008
  • Place of Presentation: Taipei, Taiwan
  • Year and Date: 2008-09-15
• [Presentation] Contributions of Nano- and Micro-Technologies to Liver Tissue Engineering, 招待講演 (2008)
  • Author(s): Y. Sakai
  • Organizer: CNBI-CNSI 合同ナノテクシンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2008-08-25
• [Presentation] 肝細胞培養における工学的外部環境の寄与-三次元化・酸素供給-, 招待講演 (2008)
  • Author(s): 酒井康行, 黄紅雲, 花田三四郎, 西川昌輝, 小森喜久夫, 小島伸彦, 山本貴富喜,藤井輝夫
  • Organizer: 第15回肝細胞研究会
  • Place of Presentation: 静岡
  • Year and Date: 2008-06-17
• [Presentation] 大型肝組織再構築のための担体デザイン・三次元造形・前駆細胞育成, 招待講演 (2008)
  • Author(s): 酒井康行, 黄紅雲, 大泉俊輔, 水野篤志, 太田勝次, 小島伸彦, 新野俊樹
  • Organizer: 第7回日本再生医療学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-03-21
• [Presentation] マウスES細胞からの肝細胞分化誘導のための新規培養系 (2008)
  • Author(s): 高橋亨, 寺谷工, 西川昌輝, 藤井輝夫, 落谷孝広, 酒井康行
  • Organizer: 化学工学会第73回大会
  • Place of Presentation: 静岡(口頭発表)
  • Year and Date: 2008-03-17
• [Presentation] 胎生後期ラット胎児肝細胞の三次元培養と液性因子による成熟化の検討 (2008)
  • Author(s): 花田 三四郎, 酒井 康行
  • Organizer: 第7回日本再生医療学会総会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2008-03-13
• [Book] 第18章, 血流導入型体内埋め込み組織の構築, 細胞治療・再生医療のための培養システム (紀ノ岡正博・酒井康行監修) (2010)
  • Author(s): 酒井康行, 新野俊樹 (分担執筆)
  • Publisher: シーエムシー出版
• [Book] 第18章,血流導入型体内埋め込み組織の構築,pp.159-166,細胞治療・再生医療のための培養システム紀ノ岡正博, 酒井康行監修 (2010)
  • Author(s): 酒井康行, 新野俊樹(分担執筆)
  • Total Pages: 320
  • Publisher: シーエムシー出版,大阪
• [Book] 未分化肝細胞を用いた異所的な肝組織の再構築とその制御, 遺伝子医学MOOK別冊, 進み続ける細胞移植治療の実際―再生医療の実現に向けた科学・技術の周辺要素の理解― (2008)
  • Author(s): 小島伸彦, 酒井康行
  • Publisher: メディカルDo