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Development and application of cell-free molecular display system

Research Project

Project/Area Number 19360373
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Biofunction/Bioprocess
Research InstitutionNagoya University

Principal Investigator

NAKANO Hideo  Nagoya University, 生命農学研究科, 教授 (00237348)

Co-Investigator(Kenkyū-buntansha) IWASAKI Yugo  名古屋大学, 大学院・生命農学研究科, 准教授 (50273214)
KOJIMA Takaaki  名古屋大学, 大学院・生命農学研究科, 助教 (40509080)
河原崎 泰昌  静岡県立大学, 食品栄養科学部, 准教授 (80303585)
中嶋 光敏  筑波大学, 大学院・生命環境科学研究科・教授, 教授 (10343815)
Co-Investigator(Renkei-kenkyūsha) NAKAJIMA Mitsutoshi  筑波大学, 大学院・生命環境科学研究科, 教授 (10343815)
Project Period (FY) 2007 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000)
Fiscal Year 2010: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2009: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2008: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2007: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
KeywordsエマルジョンPCR / 無細胞タンパク質合成系 / マイクロチャネル乳化ペプチド / ビーズディスプレイ / マイクロチャネル乳化 / ペプチド / ビーズデイスプレイ
Research Abstract

A novel biomolecule selection system named 'Bead Display' that displays DNA and its encoding protein on the surface of the beads has been developed. Since the system can utilize cell sorter that can analyze and select samples, ultra high-throughput assay at most 10 million samples per an hour can be theoretically possible. As an example, selection of peptides, enzyme, or analysis of DNA binding motifs were successfully demonstrated.

Report

(6 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report   Self-evaluation Report ( PDF )
  • 2008 Annual Research Report
  • 2007 Annual Research Report
  • Research Products

    (47 results)

All 2011 2010 2009 2008 2007 Other

All Journal Article (12 results) (of which Peer Reviewed: 10 results) Presentation (32 results) Patent(Industrial Property Rights) (3 results)

  • [Journal Article] Analysis of DNA-Protein Interaction Analysis.2011

    • Author(s)
      Kojima, T., Nakano, H.GLOBE
    • Journal Title

      PCR Protocols, Methods in Molecular Biology J. Park (ed.) 687

      Pages: 307-317

    • Related Report
      2010 Final Research Report
  • [Journal Article] GLOBE : Analysis of DNA-Protein Interaction Analysis.2011

    • Author(s)
      Kojima, T., Nakano, H.
    • Journal Title

      PCR Protocols, Methods in Molecular Biology J.Park (ed.)

      Volume: 687 Pages: 307-317

    • Related Report
      2010 Annual Research Report
  • [Journal Article] High-throughput Screening of DNA Binding Sites for Transcription Factor AmyR from Aspergillus nidulans Using DNA Beads Display System.2010

    • Author(s)
      Kojima, T., Hashimoto, Y., Kato, M., Kobayashi, T., Nakano, H.
    • Journal Title

      J.Biosci.Bioeng. 109

      Pages: 519-512

    • NAID

      110007641894

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Directed Evolution of Angiotensin II-inhibiting Peptides Using Microbeads Display.2010

    • Author(s)
      Gan, R., Furuzawa, S., Kojima, T., Kanie, K., Kato, R., Okochi, M., Honda, H., Nakano, H.
    • Journal Title

      J.Biosci.Bioeng. 109

      Pages: 411-417

    • NAID

      110007618908

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Directed Evolution of Angiotensin II-inhibiting Peptides Using Microbeads Display.2010

    • Author(s)
      Gan, R., Furuzawa, S., Kojima, T., Kanie, K., Kato, R., Okochi, M., Honda, H., Nakano, H.
    • Journal Title

      J.Biosci.Bioeng.

      Volume: 109 Pages: 411-417

    • NAID

      110007618908

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] (2010) High-throughput Screening of DNA Binding Sites for Transcripti on Factor AmyR from Aspergillus nidulans Using DNA Beads Display System.2010

    • Author(s)
      Kojima, T., Hashimoto, Y., Kato, M., Kobayashi, T., Nakano, H.
    • Journal Title

      J.Biosci.Bioeng.

      Volume: 109 Pages: 519-512

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Directed Evolution of Angiotensin II-inhibiting Peptides Using Microbeads Display2010

    • Author(s)
      Gan, R., Furuzawa, S., Kojima, T., Kanie, K., Kato, R., Okochi, M., Honda, H. Nakano, H
    • Journal Title

      J.Biosci.Bioeng 109

      Pages: 411-417

    • NAID

      110007618908

    • Related Report
      2009 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] Directed Evolution of Angiotensin II-inhibiting Peptides Using Microbeads Display2010

    • Author(s)
      Gan, R., Furuzawa, S., Kojima, T., Kanie, K., Kato, R., Okochi, M., Honda, H., Nakano, H.
    • Journal Title

      J.Biosci.Bioeng. 109

      Pages: 411-417

    • NAID

      110007618908

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] エマルジョンPCRが拓く分子間相互作用ハイスループットスクリーニング2009

    • Author(s)
      兒島孝明, 甘睿, 中野秀雄
    • Journal Title

      生物工学会誌 87

      Pages: 283-286

    • NAID

      110007338553

    • Related Report
      2009 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] Microbeads Display of Proteins Using Emulsion PCR and Cell-Free Protein2008

    • Author(s)
      Rui Gan, Yumiko Yamanaka, Takaaki Kojima, Hideo Nakano
    • Journal Title

      Synthesis.Biotechnol.Prog. 24

      Pages: 1107-1114

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Microbeads display of proteins using emulsion PCR and cell-free protein synthesis2008

    • Author(s)
      Gan, R, Yamanaka, Y, Kojima, T, Nakano, H.
    • Journal Title

      Biotechnol.Prog. 24

      Pages: 1107-1114

    • Related Report
      2009 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] High-throughput Screening of DNA Binding Sites for Transcription Factor AmyR from Aspergillus nidulans Using DNA Beads Display System

    • Author(s)
      Kojima, T., Hashimoto, Y., Kato, M., Kobayashi, T. Nakano, H.
    • Journal Title

      J.Biosci.Bioeng (in press)

    • NAID

      110007641894

    • Related Report
      2009 Self-evaluation Report
    • Peer Reviewed
  • [Presentation] Beads display : A Novel High-throughput Screening Technology to Explore Functional Biomolecules.2010

    • Author(s)
      見島孝明, 中野秀雄
    • Organizer
      The Eleventh China-Japan-Korea Joint Symposium on Enzyme Engineering
    • Place of Presentation
      Chengdu, China
    • Year and Date
      2010-11-06
    • Related Report
      2010 Annual Research Report 2010 Final Research Report
  • [Presentation] ビーズディスプレイ法によるチロシンキナーゼCsk新規活性検出法の確立2010

    • Author(s)
      長屋貴士、兒島孝明、中野秀雄
    • Organizer
      日本農芸化学会中部支部第159回例会
    • Place of Presentation
      名古屋
    • Year and Date
      2010-10-30
    • Related Report
      2010 Annual Research Report
  • [Presentation] グルタルアルデヒドを用いたビーズディスプレイ法におけるDNA・蛋白質複合体安定化法の確立2010

    • Author(s)
      三上友美子、松田英樹、松井大吾、兒島孝明、中野秀雄
    • Organizer
      日本農芸化学会中部支部第159回例会
    • Place of Presentation
      名古屋
    • Year and Date
      2010-10-30
    • Related Report
      2010 Annual Research Report
  • [Presentation] マイクロビーズディスプレイと無細胞タンパク質合成系を用いた糖鎖結合性タンパク質の新規解析法2010

    • Author(s)
      酒井謙、兒島孝明、山川奈緒、西浦佑二、田中浩士、高橋孝志、佐藤ちひろ、北島健、中野秀雄
    • Organizer
      日本農芸化学会中部支部第159回例会
    • Place of Presentation
      名古屋
    • Year and Date
      2010-10-30
    • Related Report
      2010 Annual Research Report
  • [Presentation] 無細胞タンパク質合成系とビーズディスプレイ法を用いた糖鎖結合性タンパク質の解析法2010

    • Author(s)
      酒井謙、兒島孝明、山川奈緒、西浦佑二、田中浩士、高橋孝志、佐藤ちひろ、北島健、中野秀雄
    • Organizer
      日本生物工学会
    • Place of Presentation
      宮崎
    • Year and Date
      2010-10-28
    • Related Report
      2010 Annual Research Report
  • [Presentation] ビーズディスプレイ法におけるDNA-蛋白質複合体の安定化2010

    • Author(s)
      三上友美子、松田英樹、松井大吾、兒島孝明、中野秀雄
    • Organizer
      日本生物工学会
    • Place of Presentation
      宮崎
    • Year and Date
      2010-10-28
    • Related Report
      2010 Annual Research Report
  • [Presentation] 無細胞蛋白質合成系を用いた1分子・1細胞由来mRNAの解析技術2010

    • Author(s)
      中野秀雄、見島孝明
    • Organizer
      日本生物工学会
    • Place of Presentation
      宮崎
    • Year and Date
      2010-10-27
    • Related Report
      2010 Annual Research Report
  • [Presentation] Bead Display : A High-throughput Screening Technology of Functional Proteins and DNAs2010

    • Author(s)
      中野秀雄
    • Organizer
      International Symposium, Annual Meeting of The Korean Society for Microbiology and Biotechnology
    • Place of Presentation
      韓国 ソウル
    • Year and Date
      2010-06-25
    • Related Report
      2010 Final Research Report
  • [Presentation] Bead Display : A High-throughput Screening Technology of Functional Proteins and DNAs2010

    • Author(s)
      Hideo NAKANO
    • Organizer
      International Symposium & Annual Meeting of The Korean Society for Microbiology and Biotechnology
    • Place of Presentation
      韓国 ソウル
    • Year and Date
      2010-06-25
    • Related Report
      2010 Annual Research Report
  • [Presentation] ビーズディスプレイ法におけるDNA-蛋白質複合体の安定化2010

    • Author(s)
      三上友美子, 松田英樹, 松井大悟, 兒島孝明, 中野秀雄
    • Organizer
      日本農芸化学会
    • Place of Presentation
      東京
    • Year and Date
      2010-03-29
    • Related Report
      2009 Annual Research Report 2009 Self-evaluation Report
  • [Presentation] ビーズディスプレイ法におけるDNA-蛋白質複合体の安定化2010

    • Author(s)
      三上友美子、松田英樹、松井大悟、見島孝明、中野秀雄
    • Organizer
      日本生物工学会2010年62回大会
    • Place of Presentation
      宮崎
    • Related Report
      2010 Final Research Report
  • [Presentation] DNAのビーズディスプレイ法を用いたDNA-酵母転写因子相互作用検出システム2009

    • Author(s)
      王ハン輝、見島孝明、中野秀雄
    • Organizer
      日本生物工学会
    • Place of Presentation
      名古屋
    • Year and Date
      2009-09-22
    • Related Report
      2010 Final Research Report 2009 Annual Research Report
  • [Presentation] エマルジョン培養法による分泌酵素産生菌の選択的濃縮2009

    • Author(s)
      長尾伸人、安東大介、小島晃代、河原崎泰昌、見島孝明、中野秀雄
    • Organizer
      日本生物工学会
    • Place of Presentation
      名古屋
    • Year and Date
      2009-09-22
    • Related Report
      2010 Final Research Report 2009 Annual Research Report 2009 Self-evaluation Report
  • [Presentation] ビーズディスプレイ法を用いたリパーゼのハイスループットスクリーニング法の開発2009

    • Author(s)
      松井大悟、浅賀由香理、甘睿、兒島孝明、中野秀雄
    • Organizer
      日本生物工学会
    • Place of Presentation
      名古屋
    • Year and Date
      2009-09-22
    • Related Report
      2010 Final Research Report 2009 Annual Research Report 2009 Self-evaluation Report
  • [Presentation] High-throughput Screening of DNA Binding Sites for Transcription Factor, AmyR, from Aspergillus nidulans Using Beads Display System of DNA2008

    • Author(s)
      Tadakaaki Kojima, Yoko Hashimoto, Masashi Kato, Tetsuya Kobayashi, Hideo Nakano
    • Organizer
      4th Korea-Japan Workshop on Combinatorial Bioengineering
    • Place of Presentation
      Seoul
    • Year and Date
      2008-11-21
    • Related Report
      2009 Self-evaluation Report
  • [Presentation] Beads display : a Novel Technology to Explore Functional Biomolecules2008

    • Author(s)
      Hideo Nakano
    • Organizer
      4th Korea-Japan Workshop on Combinatorial Bioengineering
    • Place of Presentation
      Seoul
    • Year and Date
      2008-11-21
    • Related Report
      2009 Self-evaluation Report
  • [Presentation] Beads Display System of Proteins Using Emulsion PCR and Cell-free Protein Synthesis2008

    • Author(s)
      Takaaki Kojima, Rui Gan, Yumiko Yamanaka, Hideo Nakano
    • Organizer
      12th KOREAN PEPTIDE-PROTEIN SOCIETY SYMPOSIUM
    • Place of Presentation
      Seoul
    • Year and Date
      2008-11-20
    • Related Report
      2009 Self-evaluation Report
  • [Presentation] Genotype-Phenotype Coupling Screening of DNA2008

    • Author(s)
      Rui GAN, Yumiko YAMANAKA, Hideo NAKANO
    • Organizer
      Molecules Displayed on Microbeads by Using Cell-free Protein Synthesis Biocat2008
    • Place of Presentation
      ハンブルグ、ドイツ
    • Year and Date
      2008-09-03
    • Related Report
      2010 Final Research Report
  • [Presentation] Genotype-Phenotype Coupling Screening of DNA Molecules Displayed on Microbeads by Using Cell-free Protein Synthesis2008

    • Author(s)
      Rui GAN, Yumiko YAMANAKA, Hideo NAKANO
    • Organizer
      Biocat2008
    • Place of Presentation
      ハンブルグ、ドイツ
    • Year and Date
      2008-09-03
    • Related Report
      2008 Annual Research Report
  • [Presentation] Genotype-Phenotype Coupling Screening of DNA Molecules Displayed on Microbeads by Using Cell-free Protein2008

    • Author(s)
      Rui GAN, Yumiko YAMANAKA, Hideo NAKANO
    • Organizer
      Synthesis Biocat2008
    • Place of Presentation
      Hamburg Germany
    • Year and Date
      2008-09-01
    • Related Report
      2009 Self-evaluation Report
  • [Presentation] DNAのビーズディスプレイ法を用いたDNA-転写因子間相互作用検出法2008

    • Author(s)
      兒島孝明, 橋本陽子, 加藤雅士, 小林哲夫, 中野秀雄
    • Organizer
      日本生物工学会
    • Place of Presentation
      仙台
    • Year and Date
      2008-08-28
    • Related Report
      2009 Self-evaluation Report
  • [Presentation] DNAのビーズディスプレイ法を用いたDNA-転写因子間相互作用検出法2008

    • Author(s)
      兒島孝明, 他
    • Organizer
      日本生物工学会平成20年度大会
    • Place of Presentation
      仙台
    • Year and Date
      2008-08-28
    • Related Report
      2008 Annual Research Report
  • [Presentation] 無細胞蛋白質合成系を用いたマイクロビーズ上での分子ディスプレイ法の開発2008

    • Author(s)
      古澤聖司, Rui GAN, 中野秀雄
    • Organizer
      日本農芸化学会
    • Place of Presentation
      名古屋
    • Year and Date
      2008-03-28
    • Related Report
      2009 Self-evaluation Report
  • [Presentation] Microbeads Display of Proteins Using Emulsion PCR and Cell-free Protein Synthesis2008

    • Author(s)
      Rui GAN, Yumiko YAMANAKA, Takaaki KOJIMA, Hideo NAKANO
    • Organizer
      日本農芸化学会
    • Place of Presentation
      名古屋
    • Year and Date
      2008-03-28
    • Related Report
      2009 Self-evaluation Report
  • [Presentation] 無細胞蛋白質合成系を用いたマイクロビーズ上での分子ディスプレイ法の開発2008

    • Author(s)
      古澤聖司, Rui GAN、中野秀雄
    • Organizer
      日本農芸化学会2008年度大会
    • Place of Presentation
      名古屋
    • Year and Date
      2008-03-27
    • Related Report
      2010 Final Research Report 2007 Annual Research Report
  • [Presentation] Microbeads Display of Proteins Using Emulsion PCR and Cell-free Protein Synthesis2008

    • Author(s)
      Rui GAN, Yumiko YAMANAKA, Hideo NAKANO
    • Organizer
      日本農芸化学会2008年度大会
    • Place of Presentation
      名古屋
    • Year and Date
      2008-03-27
    • Related Report
      2010 Final Research Report 2007 Annual Research Report
  • [Presentation] High-throughput Screening of DNA Binding Sites for Transcription Factor, AmyR, from Aspergillus nidulans Using Beads Display System of DNA.2008

    • Author(s)
      見島孝明, 橋本陽子, 加藤雅士, 小林哲夫, 中野秀雄
    • Organizer
      4th Korea-Japan Workshop on Combinatorial Bioengineering
    • Place of Presentation
      Seoul
    • Related Report
      2010 Final Research Report
  • [Presentation] DNAのビーズディスプレイ法を用いたDNA-転写因子問相互作用検出法2008

    • Author(s)
      見島孝明, 橋本陽子、加藤雅士、小林哲夫、中野秀雄
    • Organizer
      日本生物工学会平成20年度大会
    • Place of Presentation
      仙台
    • Related Report
      2010 Final Research Report
  • [Presentation] DNAのビーズディスプレイ法を用いたDNA転写因子問相互作用検出法2007

    • Author(s)
      橋本陽子、見島孝明、加藤雅士、小林哲夫、中野秀雄
    • Organizer
      日本生物工学会平成19年度大会
    • Place of Presentation
      広島
    • Year and Date
      2007-09-26
    • Related Report
      2010 Final Research Report
  • [Presentation] DNAのビーズディスプレイ法を用いたDNA転写因子間相互作用検出法2007

    • Author(s)
      橋本陽子, 他
    • Organizer
      日本生物工学会平成19年度大会
    • Place of Presentation
      広島
    • Year and Date
      2007-09-26
    • Related Report
      2007 Annual Research Report
  • [Presentation] 無細胞蛋白質合成系を用いたBurkholderia cepacia由来リパーゼの基質特異性の改変2007

    • Author(s)
      合田陽平, 他
    • Organizer
      日本生物工学会平成19年度大会
    • Place of Presentation
      広島
    • Year and Date
      2007-09-26
    • Related Report
      2007 Annual Research Report
  • [Presentation] DNAのビーズディスプレイ法を用いたDNA転写因子間相互作用検出法2007

    • Author(s)
      橋本陽子, 兒島孝明, 加藤雅士, 小林哲夫, 中野秀雄
    • Organizer
      日本生物工学会
    • Place of Presentation
      東広島
    • Year and Date
      2007-09-18
    • Related Report
      2009 Self-evaluation Report
  • [Patent(Industrial Property Rights)] 分子ディスプレイ法およびその用途2008

    • Inventor(s)
      中野秀雄古澤聖司
    • Industrial Property Rights Holder
      国立大学法人名古屋大学
    • Industrial Property Number
      2008-018324
    • Filing Date
      2008-01-29
    • Related Report
      2010 Final Research Report
  • [Patent(Industrial Property Rights)] 分子ディスプレイ法及びその用途2008

    • Inventor(s)
      中野秀雄, 古澤聖司
    • Industrial Property Rights Holder
      名古屋大学
    • Filing Date
      2008-01-29
    • Related Report
      2009 Self-evaluation Report
  • [Patent(Industrial Property Rights)] 分子ディスプレイ法およびその用途2008

    • Inventor(s)
      中野秀雄, 古澤聖司
    • Industrial Property Rights Holder
      名古屋大学
    • Industrial Property Number
      2008-018324
    • Filing Date
      2008-01-29
    • Related Report
      2007 Annual Research Report

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Published: 2007-04-01   Modified: 2016-04-21  

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