Development and application of cell-free molecular display system
Project/Area Number |
19360373
|
Research Category |
Grant-in-Aid for Scientific Research (B)
|
Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Biofunction/Bioprocess
|
Research Institution | Nagoya University |
Principal Investigator |
NAKANO Hideo Nagoya University, 生命農学研究科, 教授 (00237348)
|
Co-Investigator(Kenkyū-buntansha) |
IWASAKI Yugo 名古屋大学, 大学院・生命農学研究科, 准教授 (50273214)
KOJIMA Takaaki 名古屋大学, 大学院・生命農学研究科, 助教 (40509080)
河原崎 泰昌 静岡県立大学, 食品栄養科学部, 准教授 (80303585)
中嶋 光敏 筑波大学, 大学院・生命環境科学研究科・教授, 教授 (10343815)
|
Co-Investigator(Renkei-kenkyūsha) |
NAKAJIMA Mitsutoshi 筑波大学, 大学院・生命環境科学研究科, 教授 (10343815)
|
Project Period (FY) |
2007 – 2010
|
Project Status |
Completed (Fiscal Year 2010)
|
Budget Amount *help |
¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000)
Fiscal Year 2010: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2009: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2008: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2007: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
|
Keywords | エマルジョンPCR / 無細胞タンパク質合成系 / マイクロチャネル乳化ペプチド / ビーズディスプレイ / マイクロチャネル乳化 / ペプチド / ビーズデイスプレイ |
Research Abstract |
A novel biomolecule selection system named 'Bead Display' that displays DNA and its encoding protein on the surface of the beads has been developed. Since the system can utilize cell sorter that can analyze and select samples, ultra high-throughput assay at most 10 million samples per an hour can be theoretically possible. As an example, selection of peptides, enzyme, or analysis of DNA binding motifs were successfully demonstrated.
|
Report
(6 results)
Research Products
(47 results)
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[Journal Article] Directed Evolution of Angiotensin II-inhibiting Peptides Using Microbeads Display.2010
Author(s)
Gan, R., Furuzawa, S., Kojima, T., Kanie, K., Kato, R., Okochi, M., Honda, H., Nakano, H.
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Journal Title
J.Biosci.Bioeng. 109
Pages: 411-417
NAID
Related Report
Peer Reviewed
-
[Journal Article] Directed Evolution of Angiotensin II-inhibiting Peptides Using Microbeads Display.2010
Author(s)
Gan, R., Furuzawa, S., Kojima, T., Kanie, K., Kato, R., Okochi, M., Honda, H., Nakano, H.
-
Journal Title
J.Biosci.Bioeng.
Volume: 109
Pages: 411-417
NAID
Related Report
Peer Reviewed
-
-
[Journal Article] Directed Evolution of Angiotensin II-inhibiting Peptides Using Microbeads Display2010
Author(s)
Gan, R., Furuzawa, S., Kojima, T., Kanie, K., Kato, R., Okochi, M., Honda, H. Nakano, H
-
Journal Title
J.Biosci.Bioeng 109
Pages: 411-417
NAID
Related Report
Peer Reviewed
-
[Journal Article] Directed Evolution of Angiotensin II-inhibiting Peptides Using Microbeads Display2010
Author(s)
Gan, R., Furuzawa, S., Kojima, T., Kanie, K., Kato, R., Okochi, M., Honda, H., Nakano, H.
-
Journal Title
J.Biosci.Bioeng. 109
Pages: 411-417
NAID
Related Report
Peer Reviewed
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