Molecular analysis of recombination mediators from fission yeast

• Project/Area Number: 19370002
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Genetics/Genome dynamics
• Research Institution: Yokohama City University
• Principal Investigator: IWASAKI Hiroshi Yokohama City University, 大学院・生命ナノシステム科学研究科 (60232659)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000); Fiscal Year 2008: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2007: ¥13,520,000 (Direct Cost: ¥10,400,000、Indirect Cost: ¥3,120,000)
• Keywords: 相同組換え / 複製フォーク / DNA二重鎖切断修復 / Rad51リコンビナーゼ / ATPase / 試験管内再構成系 / ホリデイ構造 / DNA結合タンパク質 / 分裂酵母 / Rad22 / DNA二重鎖切断 / DNA修復 / Swi5 / メディエーター / Rad51 / Rhp51

Research Abstract

本研究は、分裂酵母の2種類の組換えメディエーター(Rad22, Swi5-Sfr1)が、協調してRad51リコンビナーゼによるDNA鎖交換反応を促進することをin vitroで証明した。この時、Swi5-Sfr1複合体はRad51のATP依存的DNA結合能の上昇を引き起こし、Rad22はRPAにコートされた単鎖DNAにRad51をリクルートするという、2つのメディエーターの異なる役割分担による詳細な分子メカニズムが明らかになった。

Research Products

• [Journal Article] RAD6-RAD18-RAD5 pathway-dependent tolerance to chronic low-dose UV light (2009)
  • Author(s): Hishida T, Kubota Y, Carr AM and Iwasaki H
  • Journal Title: Nature 457 Pages: 612-615
  • Peer Reviewed
• [Journal Article] RAD6-RAD18-RAD5 pathway-dependent tolerance to chronic low-dose UV light. (2009)
  • Author(s): Hishida T, Kubota Y, Carr AM, Iwasaki H.
  • Journal Title: Nature 457 Pages: 612-615
  • Peer Reviewed
• [Journal Article] A DNA polymerase α accessory protein, Mcl1, is required for propagation of centromere structures in fission yeast (2008)
  • Author(s): Natsume T, Tsutsui Y, Takashi T, Dunleavy EM, Pidoux AL, Iwasaki H, Shirahige K, Allshire RC, and Yamao F
  • Journal Title: PLoS One 3 Pages: 2221-2221
  • Peer Reviewed
• [Journal Article] Molecular characterization of the Schizosaccharomyces pombe nip1^+/ctp1^+ gene in DNA double strand break repair in association with the Mre11-Rad50-Nbs1 complex (2008)
  • Author(s): Akamatsu Y, Murayama Y, Yamada T, Nakazaki T, Tsutsui Y, Ohta K and Iwasaki H
  • Journal Title: Mol. Cell. Biol 28 Pages: 3639-3651
  • Peer Reviewed
• [Journal Article] Mus81 is essential for sister chromatid recombination at broken replication forks (2008)
  • Author(s): Roseaulin L, Yamada Y, Tsutsui Y, Russell P, Iwasaki H, and Arcangioli B
  • Journal Title: EMBO J 27 Pages: 1378-1387
  • Peer Reviewed
• [Journal Article] Reconstitution of DNA strand exchange mediated by Rhp51 recombinase and two mediators (2008)
  • Author(s): Kurokawa Y, Murayama Y, Haruta-Takahashi N, Urabe I and Iwasaki H
  • Journal Title: PLoS Biol 6
  • Peer Reviewed
• [Journal Article] Formation and branch migration of Holliday junctions mediated by eukaryotic recombinases (2008)
  • Author(s): Murayama Y, Kurokawa Y, Mayanagi K, and Iwasaki H
  • Journal Title: Nature 451 Pages: 1018-1021
  • Peer Reviewed
• [Journal Article] Fission yeast Swi5 protein, a novel DNA recombination mediator (2008)
  • Author(s): Haruta N, Akamatsu Y, Tsutsui Y, Kurokawa Y, Murayama Y, Arcangioli B, Iwasaki H
  • Journal Title: DNA Repair (Amst) 7 Pages: 1-9
  • Peer Reviewed
• [Journal Article] Reconstitution of DNA strand exchange mediated by Rhp51 recombinase and two mediators (2008)
  • Author(s): Kurokawa Y, Murayama Y, Haruta-Takahashi N, Urabe I, Iwasaki H.
  • Journal Title: PLoS Biol 6
  • Peer Reviewed
• [Journal Article] Molecular characterization of the Schizosaccharomyces pombe nipl+/ctp1+ gene in DNA double strand break repair in association with the Mrell-Rad50-Nbs1 complex (2008)
  • Author(s): Akamatsu Y, Murayama Y, Yamada T, Nakazaki T, Tsutsui Y, Ohta K, Iwasaki H.
  • Journal Title: Mol Cell Biol 28 Pages: 3639-3651
  • Peer Reviewed
• [Journal Article] Reconstitution of DNA strand exchange mediated by Rhp51 recombinase and two mediators (2008)
  • Author(s): Kurokawa Y, Murayama Y, Haruta-Takahashi N, Urabe I, Iwasaki H.
  • Journal Title: PLoS Biol 6(印刷中)
  • Peer Reviewed
• [Journal Article] Molecular characterization of the Schizosaccharomyces pombe nip1+/ctp1+gene in DNA double strand break repair in association with the Mre11-Rad50-Nbs1 complex (2008)
  • Author(s): Akamatsu Y, Murayama Y, Yamada T, Nakazaki T, Tsutsui Y, Ohta K, Iwasaki H.
  • Journal Title: Mol Cell Biol (印刷中)
  • Peer Reviewed
• [Journal Article] Formation and branch migration of Holliday junctions mediated by eukaryotic recombinases. (2008)
  • Author(s): Murayama Y, Kurokawa Y, Mayanagi K, Iwasaki H.
  • Journal Title: Nature 451 Pages: 1018-21
  • Peer Reviewed
• [Journal Article] Fission yeast Swi5/Sfr1 and Rhp55/Rhp57 differentially regulate Rhp51-dependent recombination outcomes (2007)
  • Author(s): Akamatsu Y, Tsutsui Y, Morishita T, Siddique, MDP, Kurokawa, Y. Ikeguchi M, Yamao F, Arcangioli B, and Iwasaki H
  • Journal Title: EMBO J. 26 Pages: 1352-1362
  • Peer Reviewed
• [Presentation] Biochemical analysis of DNA strand exchange mediated by the fission yeast recombinase Rhp51 and its auxiliary proteins (2008)
  • Author(s): 岩崎博史
  • Organizer: 2008年度日本分子生物学会日本生化学会合同年会4S11シンポジウム 染色体サイクルの制御機構
  • Place of Presentation: 神戸ポートアイランド
• [Presentation] Biochemical analyses of the Swi5-Sfr1 complex on the two different recombainses in fission yeast (2008)
  • Author(s): Iwasaki H.
  • Organizer: The 6^h international 3R symposium
  • Place of Presentation: つま恋リゾート. 静岡
• [Presentation] Stimulation of Rhp51- (Rad51-) and Dmc1-mediated DNA strand exchanges by the Swi5-Sfr1 complex (2008)
  • Author(s): Iwasaki H.
  • Organizer: The 1^<st> GCOE symposium on "Chromosome Biology"
  • Place of Presentation: 東京工業大学.東京
• [Presentation] 相同組換えにおけるDNA鎖交換の反応機構 (2008)
  • Author(s): 岩崎博史
  • Organizer: 2008年度日本遺伝学会大80大会Workshop 11染色体機能とDNAトランスアクション
  • Place of Presentation: 名古屋大学工学部
• [Presentation] DNA strand exchange reactions mediated by the fission yeast recombinase Rhp51 (spRad51) and its mediators, Rad22 (spRad52) and Swi5-Sfr1 (2008)
  • Author(s): Iwasaki H.
  • Organizer: EMBO Conference Series on Recombination Mechanism
  • Place of Presentation: Il Ciocco, Italy
• [Presentation] The fission yeast nip1^+ gene, which is involved in DNA double strand break repair togehter with the Mre11-Rad50-Nbs1 complex, is a functional counterpart Saccharomyces Sae2/Com1 and mammalian CtIP (2008)
  • Author(s): Akamatsu Y, Murayama Y, Yamada T, Tsutsui Y, Ohta K and Iwasaki H
  • Organizer: Keystone symposia on DNA replication and Recombination
  • Place of Presentation: Santa Fe, New Mexico, USA
• [Presentation] Biochemical analysis of DNA strand exchange mediated by the fission yeast recombinase Rhp51 and its auxiliary proteins (2008)
  • Author(s): 岩崎博史
  • Organizer: 日本分子生物学会日本生化学会合同年会シンポジウム4S11染色体サイクルの制御機
  • Place of Presentation: 神戸ポートアイランド
• [Presentation] 分裂酵母Rhp51リコンビナーゼによるDNA鎖交換反応のメカニズム (2007)
  • Author(s): 岩崎博史
  • Organizer: 日本分子生物学会BMB2007
  • Place of Presentation: 横浜パシフィコ
  • Year and Date: 2007-12-14
• [Presentation] 分裂酵母Rhp51リコンビナーゼによるDNA鎖交換反応のメカニズム (2007)
  • Author(s): 岩崎博史
  • Organizer: 日本分子生物学会日本生化学会合同年会. 4S17シンポジウム 進化的視点からとらえる相同組換え修復
  • Place of Presentation: パシフィコ横浜
• [Presentation] Biochemical analysis of DNA strand exchange reactions mediated by fission yeast recombinase Rhp51 (2007)
  • Author(s): Iwasaki H.
  • Organizer: 2^<nd> International symposium of Chromosome and Genome Stability & Instability
  • Place of Presentation: 千里ライフサイエンスセンター、大阪
• [Presentation] The Swi5-Sfr1 protein complex, a novel recombination mediator in fission yeast (2007)
  • Author(s): Iwasaki H.
  • Organizer: The 4^<th> International Fission Yeast Meeting
  • Place of Presentation: Copenhagen, Denmark
• [Presentation] Reconstitution of Rhp51 (spRad51)- dependent DNA strand exchange reaction during HR repair in fission yeast (2007)
  • Author(s): Iwasaki H.
  • Organizer: The 3rd US-Japan DNA repair meeting
  • Place of Presentation: 秋保, 宮城
• [Remarks]
  • URL: http://www.yokohama-cu.ac.jp/res/researcher/info/080207_iwasaki.html
• [Remarks]
  • URL: http://www.yokohama-cu.ac.jp/res/researcher/info/080207_iwasaki.html