Analysis of transcriptional regulatory networks in response to abioticstress in plants

• Project/Area Number: 19370016
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: 植物生理・分子
• Research Institution: The University of Tokyo
• Principal Investigator: SHINOZAKI Kazuko The University of Tokyo, 大学院・農学生命科学研究科, 教授 (30221295)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000); Fiscal Year 2008: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000); Fiscal Year 2007: ¥11,440,000 (Direct Cost: ¥8,800,000、Indirect Cost: ¥2,640,000)
• Keywords: 転写因子 / 水分ストレス / 高温ストレス / 遺伝子発現制御 / シグナル伝達系 / E3リガーゼ / ユビキチン化 / リポーター遺伝子 / 遺伝子破壊株 / マイクロアレイ / スプライシング

Research Abstract

植物は、温度や水分環境の変化に応答して自らの生育や生理状態を変化させることにより、厳しい生育環境にも耐えることができるようになる。本研究課題では、高温・水分ストレス応答で働く転写因子DREB2Aについて、それを活性化させる仕組みについて明らかにするとともに、高温・水分ストレス応答における標的遺伝子を探索し、ストレス耐性遺伝子の発現制御ネットワークを分子レベルで明らかにする。

Research Products

• [Journal Article] Arabidopsis DREB2A-interacting proteins function as RING E3 ligases and negatively regulate plant drought stress-responsive gene expression. (2008)
  • Author(s): Qin, F., Sakuma, Y., Tran, L.-S. P., Maruyama, K., Kidokoro, S., Fujita, Y., Fujita, M., Umezawa, T., Sawano, Y., Miyazono, K., Tanokura, M., Shinozaki, K, and Yamaguchi-Shinozaki, K.
  • Journal Title: Plant Cell 20 Pages: 1693-1707
  • Peer Reviewed
• [Journal Article] Functional analysis of an Arabidopsis heat shock transcription factor HsfA3 in the transcriptional cascade downstream of the DREB2A stress regulatory system (2008)
  • Author(s): Yoshida, T., Sakuma, Y., Todaka, D., Maruyama, K., Qin, F., Mizoi, J., Kidokoro, S., Fujita, Y., Shinozaki, K., and Yamaguchi-Shinozaki, K.
  • Journal Title: Biochem. Biophys. Res. Commun 368 Pages: 515-521
  • Peer Reviewed
• [Journal Article] Functional analysis of an Arabidopsis heat shock transcription factor HsfA3 in the transcriptional cascade downstream of the DREB2A stress regulatory system (2008)
  • Author(s): Yoshida, T. et al.
  • Journal Title: Biochem. Biophys. Res. Commun. 368 Pages: 515-521
  • Peer Reviewed
• [Journal Article] Arabidopsis DREB2A-interacting proteins function as RING E3 ligases and negatively regulate plant drought stress-responsive gene expression (2008)
  • Author(s): Qin, F. et al.
  • Journal Title: Plant Cell 20 Pages: 1693-1707
  • Peer Reviewed
• [Journal Article] Functional analysis of an Arabidopsis heat shock transcription factor HsfA3 in the transcriptional cascade downstream of the DREB2A stressregulatory system (2008)
  • Author(s): Takumi Yoshida
  • Journal Title: Biochemical and Biophysical Research Communications 368 Pages: 515-521
  • Peer Reviewed
• [Journal Article] Regulation and functional analysis of ZmDREB2A in response to drought and heat stresses in Zea mays L (2007)
  • Author(s): Qin, F., Kakimoto, M., Sakuma, Y., Maruyama, K., Osakabe, Y., Tran, L.-S. P., Shinozaki, K. and Yamaguchi-Shinozaki, K.
  • Journal Title: L. Plant J. 50 Pages: 54-69
  • Peer Reviewed
• [Journal Article] Regulation and functional analysis of ZmDREB2A in response to drought and heat stresses in Zeamays L (2007)
  • Author(s): Feng Qin
  • Journal Title: The Plant Journal 50 Pages: 54-69
  • Peer Reviewed
• [Presentation] Posttranscriptional modification study on an Arabidopsis transcription factor DREB2A involved in drought-and heat-inducible gene expression (2009)
  • Author(s): Qin, F., Shinozaki, K. and Yamaguchi-Shinozaki, K.
  • Organizer: 第50回日本植物生理学会年会
  • Place of Presentation: 名古屋
• [Presentation] シロイヌナズナの環境ストレス応答性転写因子DREB2Aの高温ストレス下における発現制御機構の解明 (2009)
  • Author(s): 吉田拓実
  • Organizer: 第50回日本植物生理学会年会
  • Place of Presentation: 名古屋
• [Presentation] シロイヌナズナにおける乾燥と高温の複合ストレスに応答した遺伝子発現制御機構の解明 (2009)
  • Author(s): 志村恵実
  • Organizer: 第50回日本植物生理学会年会
  • Place of Presentation: 名古屋
• [Presentation] シロイヌナズナの乾燥と高温ストレス誘導性遺伝子の発現制御機構におけるDREB2B遺伝子の機能解析 (2009)
  • Author(s): 安田奈保美
  • Organizer: 第50回日本植物生理学会年会
  • Place of Presentation: 名古屋
• [Presentation] 比較系統解析に基づいたDREB2型転写因子に共通する機能ドメインの探索 (2009)
  • Author(s): 溝井順哉
  • Organizer: 第50回日本植物生理学会年会
  • Place of Presentation: 名古屋
• [Presentation] Posttranscriptional modification study on an Arabidopsis transcription factor DREB2A involved in drought- and heat-inducible gene expression (2009)
  • Author(s): Feng Qin
  • Organizer: 第50回日本植物生理学会年会
  • Place of Presentation: 名古屋
• [Presentation] 環境耐性作物のゲノム育種 (2008)
  • Author(s): 篠崎和子
  • Organizer: 第1回アグリバイオインフォマティクス研究会
  • Place of Presentation: 東京
  • Year and Date: 2008-07-15
• [Presentation] Functional analysis of a DRIP1 (DREB2A Interacting Protein 1) homolog DRIP3 using overexpressors and RNAi silencing approaches under drought stress condition (2008)
  • Author(s): Fu, C., Qin, F., Shinozaki, K. and Yamaguchi-Shinozaki, K.
  • Organizer: Plant Genomics in China IX
  • Place of Presentation: Guangzhou, China
• [Presentation] Regulatory networks of gene expression in abiotic stress response in plants (2008)
  • Author(s): Yamaguchi-Shinozaki, K.
  • Organizer: Plant Genomics in China IX
  • Place of Presentation: Guangzhou, China
• [Presentation] シロイヌナズナの環境ストレス応答性転写因子DREB2Aの高温ストレス下における発現制御機構の解明 (2008)
  • Author(s): 吉田拓実
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸
• [Presentation] Functional analysis of Arabidopsis HsfA3 controlled by DREB2A regulatory system under heat stress (2007)
  • Author(s): Yoshida, T., Sakuma, Y., Todaka, D., Osakabe, Y., Maruyama, K., Shinozaki, K. and Yamaguchi-Shinozaki, K.
  • Organizer: 7^<th> APSRC International Symposium : Abiotic Stress and Plant Growth and Development
  • Place of Presentation: Gwangju, Korea
  • Year and Date: 2007-11-08
• [Presentation] Regulatory networks of gene expression in abiotic stress responses in Arabidopsis and rice (2007)
  • Author(s): Yamaguchi-Shinozaki, K. and Shinozaki, K.
  • Organizer: 2007 International Symposium on Plant Stress and Metabolism & Annual Meeting of the KSABC
  • Place of Presentation: Gwangju, Korea
• [Presentation] Improving abiotic stress tolerance in crops (2007)
  • Author(s): Yamaguchi-Shinozaki, K. and Shinozaki, K.
  • Organizer: Danforth Center Fall Symposium "Frontiers in Transgenesis"
  • Place of Presentation: St. Louis, USA
• [Remarks]
  • URL: http://park.itc.u-tokyo.ac.jp/pmp/index.html