| Budget Amount *help |
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2010: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2009: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2008: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2007: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
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| Research Abstract |
Here I aimed to control gene expression in targeted cells and organelle by using our laser injection method (Laser-assisted Thermal-expansion Microinjection ; LTM). I especially focused on down-regulation of targeted genes by using a unique plant Torenia fournieri, of which embryo sac is protruding from the ovule tissue. We succeeded in down-regulation of targeted genes by injecting morpholino antisense oligomers (MOs) into the protruding embryo sac. When we down-regulated LURE1 or LURE2 genes, which were candidates of pollen tube attractant genes specifically expressed in the synergid cell, pollen tube attraction was impaired. These results lead to identification of true attractant molecules LUREs. MOs were shown to be effective in plant cells as well as a protist Physarum polycephalum. Combination of laser injection with MOs was shown to be a powerful technique for down-regulation of eukaryotic cells.
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