Gene regulation of targeted cells and organelle by laser microinjection
Project/Area Number |
19370017
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
植物生理・分子
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Research Institution | Nagoya University |
Principal Investigator |
HIGASHIYAMA Tetsuya Nagoya University, 大学院・理学研究科, 教授 (00313205)
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Project Period (FY) |
2007 – 2010
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Project Status |
Completed (Fiscal Year 2010)
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Budget Amount *help |
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2010: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2009: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2008: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2007: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
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Keywords | レーザーインジェクション / マイクロインジェクション / 植物細胞 / モルフォリノアンチセンスオリゴ / 遺伝子発現制御 / モルフォリノアンセチョセンスオリゴ / 植物培養細胞 / 雌性配偶体 / 花粉管ガイダンス / 遺伝子発現阻害 / 抗体 / 細胞 / オルガネラ / 顕微操作 |
Research Abstract |
Here I aimed to control gene expression in targeted cells and organelle by using our laser injection method (Laser-assisted Thermal-expansion Microinjection ; LTM). I especially focused on down-regulation of targeted genes by using a unique plant Torenia fournieri, of which embryo sac is protruding from the ovule tissue. We succeeded in down-regulation of targeted genes by injecting morpholino antisense oligomers (MOs) into the protruding embryo sac. When we down-regulated LURE1 or LURE2 genes, which were candidates of pollen tube attractant genes specifically expressed in the synergid cell, pollen tube attraction was impaired. These results lead to identification of true attractant molecules LUREs. MOs were shown to be effective in plant cells as well as a protist Physarum polycephalum. Combination of laser injection with MOs was shown to be a powerful technique for down-regulation of eukaryotic cells.
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Report
(6 results)
Research Products
(150 results)
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[Journal Article] Defensin-like polypeptide LUREs are pollen tube attractants secreted from synergid cells2009
Author(s)
Okuda S., Tsutsui H., Shiina K., Sprunck S., Takeuchi H., Yui R., Kasahara R. D., Hamamura Y., Mizukami A., Susaki D., Kawano N., Sakakibara T., Namiki S., Itoh K., Otsuka K., Matsuzaki M., Nozaki H., Kuroiwa T., Nakano A., Kanaoka M. M., Dresselhaus T., Sasaki N., Higashiyama T
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Journal Title
Nature 458
Pages: 357-361
Related Report
Peer Reviewed
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[Presentation] 花粉管誘引物質の同定2008
Author(s)
東山哲也, 奥田哲弘, 筒井大貴, 椎名恵子, 武内秀憲, 金岡雅浩, 佐々木成江
Organizer
日本植物学会第72 回大会
Place of Presentation
高知
Year and Date
2008-09-26
Related Report
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[Presentation] ミトコンドリア核内の機能領域を構成するタンパク質の解析2008
Author(s)
伊藤喜重, 泉亜紀子, 森稔幸, 前田桂, 由比良子, 堂前直, 東山哲也, 黒岩常祥, 室伏きみ子, 河野重行, 佐々木成江
Organizer
日本植物学会第72回大会
Place of Presentation
高知
Year and Date
2008-09-25
Related Report
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