Studies on the mechanism of ion distribution and re-translocation in plant tissues.

• Project/Area Number: 19370018
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: 植物生理・分子
• Research Institution: Kobe University
• Principal Investigator: MIMURA Tetsuro Kobe University, 大学院・理学研究科, 教授 (20174120)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000); Fiscal Year 2009: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2008: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2007: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
• Keywords: 葉 / 維管束 / 生体膜輸送系 / 無機栄養塩 / イオンホメオスタシス / リン酸 / シロイヌナズナ / 転流 / 生体膜輸送 / 葉肉細胞 / 無機栄養塩類 / ソース・シンク / 排水組織 / ソース・シン

Research Abstract

Molecular mechanisms of homeostasis of low molecular weight substances were studied in plant cells and tissues. We have focused on the phosphate homeostasis using Arabidopsis plants and cells, or Poplar tree. First we clarified a relationship between root architecture and environmental phosphate status. We found cooperative roles of phosphate and iron are quite important. Second, we analyzed the re-translocation system of phosphate from older leaves to younger leaves of Arabidopsis based on the gene expression. Further, we observed phosphate recovery of poplar leaves during late summer and autumn. Last, we analyzed cell and tissue metabolism of inositol phosphates during seed development.

Research Products

• [Journal Article] Generation of stable "low phytic acid" transgenic rice through antisense repression of the 1D-myo-inositol 3-phosphate synthase gene using 18-kDa oleosin promoter. (2009)
  • Author(s): Kuwano, M., Mimura, T., Takaiwa, F., Yoshida, K.T.
  • Journal Title: Plant Biotechnology J. 7(1) Pages: 96-105
  • Peer Reviewed
• [Journal Article] Seeing Is Believing : On the Use of Image Databases for Visually Exploring Plant Organelle Dynamics. (2009)
  • Author(s): Mano, S., Miwa, T., Nishikawa, S., Mimura, T., Nishimura, M.
  • Journal Title: Plant Cell Physiol. 50 Pages: 2000-2014
  • NAID: 10027344621
  • Peer Reviewed
• [Journal Article] Dynamic aspects of ion accumulation by vesicle traffic under salt stress in Arabidopsis. (2009)
  • Author(s): Hamaji, K., Nagira, M., Yoshida, K., Ohnishi, M., Oda, Y., Uemura, T., Goh, T., Sato, M.-H., Terao-Morita, M., Tasaka, M., Hasezawa, S., Nakano, A., Hara-Nishimura, I., Maeshima, M., Fukaki, H., Mimura, T.
  • Journal Title: Plant Cell Physiol. 50 Pages: 2023-2033
  • NAID: 10027344737
  • Peer Reviewed
• [Journal Article] Generation of stable "low phytic acid" transgenic rice through antisense repression of the 1D-myo-inositol 3-phosphate synthase gene using 18-kDa oleosin promoter. (2009)
  • Author(s): Kuwano M.
  • Journal Title: Plant Biotechnology Journal Volume: 7 Pages: 96-105
  • Peer Reviewed
• [Journal Article] Dynamic aspects of ion accumulation by vesicle traffic under salt stress in Arabidopsis. (2009)
  • Author(s): Hamaji K.
  • Journal Title: Plant & Cell Physiology Volume: 50 Pages: 2023-2033
  • Peer Reviewed
• [Journal Article] Seeing Is Believing : On the Use of Image Databases for Visually Exploring Plant Organelle Dynamics. (2009)
  • Author(s): Mano S.
  • Journal Title: Plant & Cell Physiology Volume: 50 Pages: 2000-2014
  • Peer Reviewed
• [Journal Article] Generation of stable "low phytic acid" transgenic rice through antisense repression of the 1D-myo-inositol 3-phosphate synthase gene using 18-kDa oleosin promoter (2009)
  • Author(s): Kuwano M.
  • Journal Title: Plant Biotechnology Journal 7 Pages: 96-105
  • Peer Reviewed
• [Journal Article] Dynamic aspects of ion accumulation by vesicle traffic under salt stress in Arabidopsis (2009)
  • Author(s): Hamaji K.
  • Journal Title: Plant & Cell Physiology 50 Pages: 2023-2033
  • Peer Reviewed
• [Journal Article] Seeing Is Believing : On the Use of Image Databases for Visually Exploring Plant Organelle Dynamics (2009)
  • Author(s): Mano S.
  • Journal Title: Plant & Cell Physiology 50 Pages: 2000-2014
  • Peer Reviewed
• [Journal Article] Localization of myo-inositol-1-phosphate synthase to the endosperm in developing seeds of Arabidopsis. (2008)
  • Author(s): Mitsuhashi, N., Kondo, M., Nakaune, S., Ohnishi, M., Hayashi, M., Hara-Nishimura, I., Richardson, A., Fukaki, H., Nishimura, M., Mimura, T.
  • Journal Title: J.Exp.Bot. 59 Pages: 3069-3076
  • Peer Reviewed
• [Journal Article] Domain II mutations in CRANE/IAA18 suppress lateral root formation and affect shoot development in Arabidopsis thaliana. (2008)
  • Author(s): Uehara, T., Okushima, Y., Mimura, T., Tasaka, M., Fukaki, H.
  • Journal Title: Plant Cell Physiol. 49 Pages: 1025-1083
  • NAID: 10027332233
  • Peer Reviewed
• [Journal Article] Deletion of a histidine-rich loop of AtMTP1, a vacuolar Zn^<2+>/H^+ antiporter of Arabidopsis thaliana, stimulates the transport activity. (2008)
  • Author(s): Kawachi, M., Kobae, Y., Mimura, T., Maeshima, M.
  • Journal Title: J.Biol.Chem. 283 Pages: 8374-8383
  • Peer Reviewed
• [Journal Article] Osmoregulation coupled with change of growth mode in Spirogyra. (2008)
  • Author(s): Yoshida, K., Ohtani, A.Mimura, T., Shimmen, T.
  • Journal Title: Functional Plant Biology 35 Pages: 580-584
  • Peer Reviewed
• [Journal Article] Proteome analysis of vacuolar membrane. (2008)
  • Author(s): Mimura, T., Ohnishi, M., Shimaoka, T., Tomizawa, K.
  • Journal Title: In "Plant Genetic Engineering vol 9 : Plant membrane and vacuolar transporters"(Ed.by Jaiwal PK.)(CABInternatonal) Pages: 301-343
  • Peer Reviewed
• [Journal Article] Localization of myo-inositol-1-phosphate synthase to the endosperm in developing seeds of Arabidopsis (2008)
  • Author(s): Mitsuhashi N.
  • Journal Title: Journal of Experimental Botany 59 Pages: 3069-3076
  • Peer Reviewed
• [Journal Article] Deletion of a histidine-rich loop of AtMTP1, a vacuolar Zn^<2+>/H^+ antiporter of Arabidopsis thaliana, stimulates the transport activity (2008)
  • Author(s): Kawachi M.
  • Journal Title: Journal of Biological Chemistry 283 Pages: 8374-8383
  • Peer Reviewed
• [Journal Article] Domain II mutations in CRANE/IAA18 suppress lateral root formation and affect shoot development in Arabidopsis thaliana (2008)
  • Author(s): Uehara T.
  • Journal Title: Plant & Cell Physiology 49 Pages: 1025-1083
  • Peer Reviewed
• [Journal Article] The plant organelles database(PODB): acollection of visualized plant organelles and protocols for plant organelle research. (2008)
  • Author(s): Mano S.
  • Journal Title: Nucleic Acid Research 36
  • Peer Reviewed
• [Journal Article] Deletion of a histidine-rich loop of AtMTPI, a vacuolar Zn2+/H+ antiporter of Arabidopsis thaliana, stimulates the transport activity. (2008)
  • Author(s): Kawachi M.
  • Journal Title: Journal of Biological Chemistry (in press)
  • Peer Reviewed
• [Journal Article] Inorganic phosphate uptake in intact vacuoles isolated from suspension cultured cells of (2007)
  • Author(s): Ohnishi, M., Mimura, T., Tsujimura, T., Mitsuhashi, M., Washitani-Nemoto, S., Maeshima, M., Martinoia, E.
  • Journal Title: Catharanthus roseus (L.) G.Don under varying Pi status. Planta 225 Pages: 711-718
  • Peer Reviewed
• [Journal Article] リン環境と植物「植物における環境と生物ストレスに対する応答」 (2007)
  • Author(s): 三村徹郎、大西美輪、深城英弘
  • Journal Title: 蛋白質核酸酵素-別冊(共立出版) 52 Pages: 625-632
• [Journal Article] Inorganic phosphate uptake in intact vacuoles isolated from suspension cultured cells of Catharanthus roseus(L.)G. Don under varying Pi status. (2007)
  • Author(s): Ohnishi M.
  • Journal Title: Planta 225 Pages: 711-718
  • Peer Reviewed
• [Presentation] Metabolome researches in plant metabolic regulation. (2010)
  • Author(s): 三村徹郎
  • Organizer: 21st International Conference on Arabidopsis Research
  • Place of Presentation: Yokohama Japan
• [Presentation] プロテオミクスによる液胞機能の解析、「植物科学におけるプロテオミクス」 (2010)
  • Author(s): 大西美輪、吉田勝久、三村徹郎
  • Organizer: 第51回日本植物生理学会年会
  • Place of Presentation: 熊本
• [Presentation] 植物体内におけるリンの取り込み,分配,蓄積の生理学、「植物はどうやってリンを見つけて,運んで,利用するか」 (2010)
  • Author(s): 三村徹郎
  • Organizer: 第51回日本植物生理学会年会
  • Place of Presentation: 熊本
• [Presentation] ポストゲノム解析から見えてきた植物細胞代謝における新しい液胞像、植物化学シンポジウム (2009)
  • Author(s): 三村徹郎, 姉川彩, 大西美輪
  • Place of Presentation: 神戸
  • Year and Date: 2009-12-17
• [Presentation] 液胞膜エンジニアリングによる植物代謝システム制御 (2009)
  • Author(s): 三村徹郎
  • Organizer: CREST「代謝調節機構解析に基づく細胞機能制御基盤技術」研究領域第2回公開シンポジウム
  • Place of Presentation: 東京(日本科学未来館)
  • Year and Date: 2009-10-16
• [Presentation] 植物液胞のポストゲノム解析 (2009)
  • Author(s): 三村徹郎
  • Organizer: 第4回メタボロームシンポジウム
  • Place of Presentation: 横浜
• [Presentation] Membrane microdomains in the plant vacuole. (2009)
  • Author(s): Yoshida Katsuhisa
  • Organizer: 3rd Asian Symposium on Plant Lipids
  • Place of Presentation: 横浜情報文化センター(神奈川県)
• [Presentation] Membrane microdomains in the plant vacuole (2009)
  • Author(s): Yoshida Katsuhisa
  • Organizer: 3rd Asian Symposium on Plant Lipids
  • Place of Presentation: 横浜
• [Presentation] シロイヌナズナ根系の栄養塩応答-リン酸と鉄の相互作用 (2008)
  • Author(s): 崎谷祐介
  • Organizer: (社)日本植物学会
  • Place of Presentation: 高知大学
  • Year and Date: 2008-09-26
• [Presentation] Post genome analysis of vacuolar function and control of plant metabolism. (2008)
  • Author(s): 三村徹郎
  • Organizer: 5th International Conference on Plant Metabolomics
  • Place of Presentation: Yokohama
• [Presentation] シロイヌナズナ葉内における物質輸送及び分配様式の解析. (2007)
  • Author(s): 竹山知華
  • Organizer: (社)日本植物学会
  • Place of Presentation: 千葉県野田市
  • Year and Date: 2007-09-09
• [Book] Photobook 植物細胞の知られざる世界 (2010)
  • Author(s): 西村幹夫・三村徹郎・西村いくこ・真野昌二監修、永野惇・桧垣匠文
  • Publisher: 化学同人
• [Book] 「基礎生物学シリーズ7 植物生理学」(三村徹郎、鶴見誠二編) (2009)
  • Author(s): 三村徹郎
  • Publisher: 化学同人
• [Book] 「水環境の今と未来 藻類と植物のできること」(川井浩史、三村徹郎 編)(水生植物の生理) (2009)
  • Author(s): 三村徹郎 村上明男
  • Publisher: 生物研究社
• [Book] 基礎生物学シリーズ7 植物生理学 (2009)
  • Author(s): 三村徹郎
  • Total Pages: 210
  • Publisher: 化学同人
• [Book] Plant membrane and vacuolar transporters (2008)
  • Author(s): 三村徹郎
  • Total Pages: 642
  • Publisher: CABInternatonal
• [Remarks] ホームページ等
  • URL: http://www.research.kobe-u.ac.jp/fsci-mimura/
• [Remarks] 研究代表者 三村徹郎