Analysis of mechanisms underlying maintenance of plant stem cells

• Project/Area Number: 19370019
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: 植物生理・分子
• Research Institution: Nara Institute of Science and Technology
• Principal Investigator: UMEDA Masaaki Nara Institute of Science and Technology, バイオサイエンス研究科, 教授 (80221810)
• Co-Investigator(Kenkyū-buntansha): OKUSHIMA Yoko 奈良先端科学技術大学院大学, バイオサイエンス研究科, 助教 (00432592)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000); Fiscal Year 2009: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000); Fiscal Year 2008: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000); Fiscal Year 2007: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
• Keywords: 幹細胞 / 細胞周期 / 分化全能性 / 細胞分裂 / 細胞分化 / サイトカニン / CDKインヒビター

Research Abstract

In this study, we focused on negative regulators of cell division to reveal molecular mechanisms underlying maintenance of plant stem cells. We found that some signal derived from very long chain fatty acid, which is produced in the epidermis of shoot apices, acts on inner cell layers to suppress stem cell division. Moreover, we developed a novel S phase-specific reporter gene by using the carboxy-terminal region of CDT1a to monitor cell proliferation in meristems.

Research Products

• [Journal Article] Two Arabidopsis cyclin A3s possess G1 cyclin-like features (2010)
  • Author(s): Takahashi, I., Kojima, S., Sakaguchi, N., Umeda-Hara, C., Umeda, M.
  • Journal Title: Plant Cell Rep. 29 Pages: 307-315
  • NAID: 120006537390
  • Peer Reviewed
• [Journal Article] The Arabidopsis cyclin-dependent kinase-activating kinase CDKF;1 is a major regulator of cell proliferation and cell expansion but is dispensable for CDKA activation (2009)
  • Author(s): Takatsuka, H., Ohno, R., Umeda, M.
  • Journal Title: Plant J. 59 Pages: 475-487
  • NAID: 120006537396
  • Peer Reviewed
• [Journal Article] Quantitative and cell type-specific transcriptional regulation of A-type cyclin-dependent kinase in Arabidopsis thaliana (2009)
  • Author(s): Adachi, S., Nobusawa, T., Umeda, M.
  • Journal Title: Dev. Biol. 329 Pages: 306-314
  • NAID: 120006537386
  • Peer Reviewed
• [Journal Article] SUMO E3 ligase HIGH PLOIDY2 regulates endocycle onset and meristem maintenance in Arabidopsis (2009)
  • Author(s): Ishida, T., Fujiwara, S., Miura, K., Stacey, N., Yoshimura, M., Schneider, K., Adachi, S., Minamisawa, K., Umeda, M., Sugimoto, K.
  • Journal Title: Plant Cell. 21 Pages: 2284-2297
  • Peer Reviewed
• [Journal Article] SUMO E3 ligase HIGH PLOIDY2 regulates endocycle onset and meristem maintenance in Arabidopsis (2009)
  • Author(s): Ishida, T.
  • Journal Title: Plant Cell 21 Pages: 2284-2297
  • Peer Reviewed
• [Journal Article] The Arabidopsis cyclin-dependent kinase-activating kinase CDKF ; 1 is a major regulator of cell proliferation and cell expansion but is dispensable for CDKA activation (2009)
  • Author(s): Takatsuka, H.
  • Journal Title: Plant J. 59 Pages: 475-487
  • Peer Reviewed
• [Journal Article] Quantitative and cell type-specific transcriptional regulation of A-type cyclin-dependent kinase in Arabidopsis thaliana (2009)
  • Author(s): Adachi, S.
  • Journal Title: Dev.Biol 329 Pages: 306-314
  • Peer Reviewed
• [Journal Article] Quantitative and cell type-specific transcriptional regulation of A-type cyclin-dependent kinase in Arabidopsis thaliana (2009)
  • Author(s): Adachi, S.
  • Journal Title: Dev. Biol. (in press)
  • Peer Reviewed
• [Journal Article] Targeted degradation of the cyclin-dependent kinase inhibitor ICK4/KRP6 by RING-type E3 ligases is essential for mitotic cell cycle progression during Arabidopsis gametogenesis (2008)
  • Author(s): Liu, J., Zhang, Y., Qin, G., Tsuge, T., Sakaguchi, N., Luo, G., Sun, K., Shi, D., Aki, S., Zheng, N., Aoyama, T., Oka, A., Yang, W., Umeda, M., Xie, Q., Gu, H., Qu, L.-J.
  • Journal Title: Plant Cell. 20 Pages: 1538-1554
  • Peer Reviewed
• [Journal Article] The rice FLATTENED SHOOT MERISTEM, encoding CAF-1 p150 subunit, is required for meristem maintenance by regulating the cell-cycle period (2008)
  • Author(s): Abe, M., Kuroshita, H., Umeda, M., Itoh, J., Nagato Y.
  • Journal Title: Dev. Biol. 319 Pages: 384-393
  • Peer Reviewed
• [Journal Article] The rice FLATTENED SHOOT MERISTEM, encoding CAF-1pl50 subunit, is required for meristem maintenance by regulating the cell-cycle period (2008)
  • Author(s): Abe, M.
  • Journal Title: Dev. Biol. 319 Pages: 384-393
  • Peer Reviewed
• [Journal Article] Targeted degradation of the cyclin-dependent kinase inhibitor ICK4/KRP6 by RING-type E3 ligases is essential for mitotic cell cycle progression during Arabidopsis gametogenesis (2008)
  • Author(s): Liu, J.
  • Journal Title: Plant Cell 20 Pages: 1538-1554
  • Peer Reviewed
• [Journal Article] The Arabidopsis D-type cyclin CYCD4 controls cell division in the stomatal lineage of the hypocotyl epidermis (2007)
  • Author(s): Kono, A., Umeda-Hara, C., Adachi, S., Nagata, N., Konomi, M., Nakagawa, T., Uchimiya, H., Umeda, M.
  • Journal Title: Plant Cell. 19 Pages: 1265-1277
  • Peer Reviewed
• [Journal Article] Potential roles for autophosphorylation, kinase activity, and abundance of a CDK-activating kinase (Ee;CDKF;1) during growth in leafy spurge (2007)
  • Author(s): Chao, W.S., Serpe, M.D., Jia, Y., Shelver, W.L., Anderson, J.V., Umeda, M.
  • Journal Title: Plant Mol. Biol. 63 Pages: 365-379
  • Peer Reviewed
• [Journal Article] The Arabidopsis D-type cyclin CYCD4 controls cell division in the stomatal lineage of the hypocotyl epidermis. (2007)
  • Author(s): Kono, A.
  • Journal Title: Plant Cell 19 Pages: 1265-1277
  • Peer Reviewed
• [Journal Article] Potential roles for autophosphorylation, kinase activity, and abundance of a CDK-activating kinase(Ee;CDKF;1)during growth inleafy spurge. (2007)
  • Author(s): Chao, W. S.
  • Journal Title: Plant Molecular Biology 63 Pages: 365-379
  • Peer Reviewed
• [Presentation] シロイヌナズナの気孔形成過程におけるCYCD4の機能解析 (2010)
  • Author(s): 奥島葉子、天野廣海、安達澄子、梅田正明
  • Organizer: 第51回日本植物生理学会年会
  • Place of Presentation: 熊本
  • Year and Date: 2010-03-19
• [Presentation] Functional divergence of CDK-activating kinases in plant development (2009)
  • Author(s): 高塚大知、梅田正明
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-10
• [Presentation] シロイヌナズナの根の成長過程における細胞周期制御因子の解析 (2009)
  • Author(s): 奥島葉子、稲本裕幸、清水皓平、梅田正明
  • Organizer: 第27回日本植物細胞分子生物学会大会
  • Place of Presentation: 神奈川
  • Year and Date: 2009-07-30
• [Presentation] Synthesis of very long chain fatty acids in the epidermis controls cell division in Arabidopsis (2009)
  • Author(s): Takashi Nobusawa, Yoko Okushima, Masaaki Umeda
  • Organizer: 20th International Conference Arabidopsis Research
  • Place of Presentation: Edinburgh
  • Year and Date: 2009-07-02
• [Presentation] 極長鎖脂肪酸合成を介したシロイヌナズナの器官形成制御機構 (2009)
  • Author(s): 信澤岳、草野都、岡咲洋三、斉藤和季、奥島葉子、梅田正明
  • Organizer: 第50回日本植物生理学会年会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-03-21
• [Presentation] 極長鎖脂肪酸合成酵素PASTICCINO2を介したシロイヌナズナの形態形成制御機構 (2008)
  • Author(s): 信澤岳、奥島葉子、河合博光、西田生郎、梅田正明
  • Organizer: 日本植物学会第72回大会
  • Place of Presentation: 高知
  • Year and Date: 2008-09-26
• [Presentation] PATICCINO2 is essential for shoot epidermis formation (2008)
  • Author(s): Takashi Nobusawa, Yoko Okushima, Hiromitsu Kawai, Ikuo Nishida, Masaaki Umeda
  • Organizer: 19th International Conference on Arabidopsis Research
  • Place of Presentation: Montreal
  • Year and Date: 2008-07-24
• [Presentation] PASTICCINO2 is essential for shoot epidermis formation (2008)
  • Author(s): 信澤 岳
  • Organizer: 19th International Conference on Arabidopsis Research
  • Place of Presentation: Montreal
  • Year and Date: 2008-07-23
• [Presentation] 器官形成を支える細胞周期制御因子の量的・質的制御機構 (2008)
  • Author(s): 梅田正明、Gyung-Tae Kim
  • Organizer: 第49回日本植物生理学会年会
  • Place of Presentation: 札幌
  • Year and Date: 2008-03-21
• [Presentation] シロイヌナズナCDK活性化キナーゼCDKF;1の機能解析 (2007)
  • Author(s): 高塚大知、梅田正明
  • Organizer: 第30回日本分子生物学会年会・第80回日本生化学会大会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-12
• [Book] in Cell cycle control and plant development: CDK phosphorylation (2007)
  • Author(s): Shimotohno, A., Umeda, M.
  • Publisher: Blackwell Publishing, Oxford
• [Book] Cell cycle control and plant development (2007)
  • Author(s): Shimotohno, A.
  • Publisher: Blackwell Publishing