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Search for mRNA elements involved in the compatibility between 5' untranslated regions and coding regions in chloroplast translation

Research Project

Project/Area Number 19370021
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 植物生理・分子
Research InstitutionNagoya City University

Principal Investigator

SUGIURA Masahiro  Nagoya City University, 大学院・システム自然科学研究科, 名誉教授 (80027044)

Co-Investigator(Kenkyū-buntansha) 黒田 洋詩  名古屋市立大学, 大学院・システム自然科学研究科, 研究員 (80381903)
中邨 真之  名古屋市立大学, 大学院・システム自然科学研究科, 研究員 (60322145)
湯川 眞希  名古屋市立大学, 大学院・システム自然科学研究科, 研究員 (00448705)
Co-Investigator(Renkei-kenkyūsha) KURODA Hiroshi  名古屋市立大学, 大学院・システム自然科学研究科, 研究員 (80381903)
NAKAMURA Masayuki  名古屋市立大学, 大学院・システム自然科学研究科, 研究員 (60322145)
YUKAWA Maki  名古屋市立大学, 大学院・システム自然科学研究科, 研究員 (00448705)
Project Period (FY) 2007 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥15,730,000 (Direct Cost: ¥12,100,000、Indirect Cost: ¥3,630,000)
Fiscal Year 2008: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
Fiscal Year 2007: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
Keywords葉緑体 / 翻訳 / タバコ / mRNA / in vitro系 / コード領域 / 5'UTR / In vitro / 適合配列
Research Abstract

植物細胞の中で光合成を行う葉緑体は盛んにタンパク質も作っている。そこで、葉緑体で有用タンパク質を生産する試みがなされたが、必ずしも成功しなかった。このことは、葉緑体のタンパク質合成の機構が動・植物の細胞質や細菌の機構とは違っていることを意味する。本研究で、葉緑体でのタンパク質合成にはメッセンジャーRNAの上流部分のみならずタンパク質のアミノ酸配列を規定する部分の一部も必要なことを明らかにした。

Report

(3 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • Research Products

    (44 results)

All 2009 2008 2007 Other

All Journal Article (10 results) (of which Peer Reviewed: 10 results) Presentation (29 results) Book (2 results) Remarks (3 results)

  • [Journal Article] Selection of synonymous codons for better expression of recombinant proteins in tobacco chloroplasts2009

    • Author(s)
      Nakamura, M. and M. Sugiura
    • Journal Title

      Plant Biotechnology (in press)

    • NAID

      10025181202

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] A new in vitro translation system for non-radioactive assay from tobacco chloroplasts : effect of pre-mRNA processing on translation in vitro2009

    • Author(s)
      Yukawa M., H. Kuroda and M. Sugiura
    • Journal Title

      Plant J 49

      Pages: 367-376

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Termination codon-dependent translation of partially overlapping ndhC-ndhK transcripts in chloroplasts2008

    • Author(s)
      Yukawa, M. and M. Sugiura
    • Journal Title

      Proc. Natl. Acad. Sci. USA 105

      Pages: 19550-19554

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Termination codon-dependent translation of partially overlapping ndhC-ndhK transcripts in chloroplasts2008

    • Author(s)
      Yukawa, Y., M. Sugiura
    • Journal Title

      Proceedings of the National Academy of Sciences of the United States of America 105

      Pages: 19549-19553

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Selection of synonymous codons for better expression of recombinant proteins in tobacco chloroplasts2008

    • Author(s)
      Nakamura, M., M. Sugiura
    • Journal Title

      Plant Biotechnology (in press)

    • NAID

      10025181202

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Translation of psbC mRNAs starts from the downstream GUG, not the upstream AUG, and requires the extended Shine-Dalgarno sequence in tobacco chloroplasts2007

    • Author(s)
      Kuroda, H., H. Suzuki, T. Kusumegi, T. Hirose, Y. Yukawa and M. Sugiura
    • Journal Title

      Plant Cell Physiol 48

      Pages: 1374-1378

    • NAID

      10027004706

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Translation efficiencies of synonymous codons are not always correlated with codon usage in tobacco chloroplasts2007

    • Author(s)
      Nakamura, M. and M. Sugiura
    • Journal Title

      Plant J 49

      Pages: 128-134

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] A survey of expressed tRNA genes in the chromosome I of Arabidopsis using an RNA polymerase III-dependent in vitro transcription system2007

    • Author(s)
      Yukawa, Y., T. Mizutani, K. Akama and M. Sugiura
    • Journal Title

      Gene 392

      Pages: 7-13

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Translation of psbC mRNAs starts from the downstream GUG, not the upstream AUG, and requires the extended Shine-Dalgarno sequence in tobacco chloroplasts2007

    • Author(s)
      Kuroda, H., H. Suzuki, T. Kusumegi, T. Hirose, Y. Yukawa and M. Sugiura
    • Journal Title

      Plant Cell Physiology 48

      Pages: 1374-1378

    • NAID

      10027004706

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The complete structure of the cucumber(Cucumis sativus L.)chloroplast genome: its composition and comparative analysis2007

    • Author(s)
      Plader, W., Y. Yukawa, M. Sugiura and S. Malepszy
    • Journal Title

      Cell. Mol. Biol. Lett. 12

      Pages: 582-594

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] 葉緑体mRNAの5'非翻訳領域の切断と翻訳効率2009

    • Author(s)
      黒田洋詩、足達由佳、湯川泰、杉浦昌弘
    • Organizer
      第50回に本植物生理学会年会
    • Place of Presentation
      名古屋
    • Related Report
      2008 Final Research Report
  • [Presentation] 葉緑体翻訳開始に関与するトランス因子2009

    • Author(s)
      黒田洋詩、杉浦昌弘
    • Organizer
      第50回に本植物生理学会年会
    • Place of Presentation
      名古屋
    • Related Report
      2008 Final Research Report
  • [Presentation] 同義コドンの選択によるコード領域の翻訳効率の変化について2009

    • Author(s)
      中邨真之、杉浦昌弘
    • Organizer
      第50回に本植物生理学会年会
    • Place of Presentation
      名古屋
    • Related Report
      2008 Final Research Report
  • [Presentation] タバコ葉緑体ndhC-K mRNAにおける終止コドンに依存した翻訳開始機構2009

    • Author(s)
      湯川眞希、杉浦昌弘
    • Organizer
      第50回に本植物生理学会年会
    • Place of Presentation
      名古屋
    • Related Report
      2008 Final Research Report
  • [Presentation] タバコ葉緑体翻訳反応における5'非翻訳領域とコード領域の適合性検証2009

    • Author(s)
      大羽祐衣、湯川眞希、杉浦昌弘、湯川泰
    • Organizer
      第50回に本植物生理学会年会
    • Place of Presentation
      名古屋
    • Related Report
      2008 Final Research Report
  • [Presentation] Unique mechanisms of translation in higher plant chloroplasts.2008

    • Author(s)
      Sugiura, M., M. Yukawa, H. Kuroda, M. Nakamura
    • Organizer
      The XVI Congress of the Federation of European Societies of Plant Biology
    • Place of Presentation
      Tampere, Finland
    • Year and Date
      2008-08-18
    • Related Report
      2008 Annual Research Report
  • [Presentation] 葉緑体mRNAの翻訳開始に必要なシス配列とトランス因子(口頭発表)2008

    • Author(s)
      黒田洋詩、杉浦昌弘
    • Organizer
      第31回日本分子生物学会年会
    • Place of Presentation
      神戸
    • Related Report
      2008 Final Research Report
  • [Presentation] 葉緑体psbD-psbC翻訳機構の解析2008

    • Author(s)
      足達由佳、黒田洋詩、杉浦昌弘、湯川泰
    • Organizer
      第31回日本分子生物学会年会
    • Place of Presentation
      神戸
    • Related Report
      2008 Final Research Report
  • [Presentation] タバコ葉緑体ndhC-ndhK mRNAにみられる翻訳終結に依存した翻訳開始機構2008

    • Author(s)
      湯川眞希、杉浦昌弘
    • Organizer
      第31回日本分子生物学会年会
    • Place of Presentation
      神戸
    • Related Report
      2008 Final Research Report
  • [Presentation] 葉緑体mRNAのタンパク質コード領域に存在する翻訳調節領域の解析2008

    • Author(s)
      中邨真之、杉浦昌弘
    • Organizer
      第31回日本分子生物学会年会
    • Place of Presentation
      神戸
    • Related Report
      2008 Final Research Report
  • [Presentation] 葉緑体rps2 mRNA の翻訳に必要なシス配列2008

    • Author(s)
      杉浦昌弘、黒田洋詩
    • Organizer
      第31回日本分子生物学会年会
    • Place of Presentation
      神戸
    • Related Report
      2008 Final Research Report
  • [Presentation] An active in vitro translation system from chloroplasts : a powerful tool to study mechanism of translation in chloroplasts2008

    • Author(s)
      Kuroda, H., M. Yukawa, H. Suzuki, Y. Yukawa and M. Sugiura
    • Organizer
      International Symposium "The Ins and Outs of Chloroplasts"
    • Place of Presentation
      Osaka
    • Related Report
      2008 Final Research Report
  • [Presentation] 葉緑体の特異な翻訳機構 : コード領域内の必須配列2008

    • Author(s)
      杉浦昌弘、中邨真之
    • Organizer
      日本植物学会第72回大会
    • Place of Presentation
      高知
    • Related Report
      2008 Final Research Report
  • [Presentation] 葉緑体ndhK遺伝子の翻訳開始点2008

    • Author(s)
      杉浦昌弘、湯川眞希
    • Organizer
      日本遺伝学会第80回大会
    • Place of Presentation
      名古屋(口頭発表)
    • Related Report
      2008 Final Research Report
  • [Presentation] Unique mechanisms of translation in higher plant chloroplasts2008

    • Author(s)
      Sugiura, M., M. Yukawa, H. Kuroda and M. Nakamura
    • Organizer
      The XVI Congress of the Federation of European Societies of Plant Biology, Tampere, Finland
    • Place of Presentation
      Selected speaker
    • Related Report
      2008 Final Research Report
  • [Presentation] タバコ葉緑体の同義コドン間の翻訳効率の測定2008

    • Author(s)
      杉浦昌弘、中邨真之
    • Organizer
      第49回日本植物生理学会年会
    • Place of Presentation
      札幌
    • Related Report
      2008 Final Research Report
  • [Presentation] 葉緑体mRNAの翻訳調節におけるタンパク質コード領域の影響2008

    • Author(s)
      中邨真之、杉浦昌弘
    • Organizer
      第49回日本植物生理学会年会
    • Place of Presentation
      札幌
    • Related Report
      2008 Final Research Report
  • [Presentation] 葉緑体in vitro翻訳系を用いたatpE mRNA翻訳開始機構の解析2008

    • Author(s)
      黒田洋詩、鈴木晴香、湯川泰、杉浦昌弘
    • Organizer
      第49回日本植物生理学会年会
    • Place of Presentation
      札幌
    • Related Report
      2008 Final Research Report
  • [Presentation] タバコ葉緑体ndhC-K mRNAにおける翻訳開始機構に関する研究2008

    • Author(s)
      湯川眞希、杉浦昌弘
    • Organizer
      第49回日本植物生理学会年会
    • Place of Presentation
      札幌(口頭発表)
    • Related Report
      2008 Final Research Report
  • [Presentation] Mechanisms of protein synthesis in higher plant chloroplasts2007

    • Author(s)
      Sugiura, M.
    • Organizer
      10th International Colloquium on Endocytobiology and Symbiosis
    • Place of Presentation
      Gmunden, Austria
    • Year and Date
      2007-09-11
    • Related Report
      2007 Annual Research Report
  • [Presentation] 葉緑体ゲノムにおけるコドン使用頻度と翻訳効率は必ずしも一致しない2007

    • Author(s)
      中邨真之、杉浦昌弘
    • Organizer
      第71回日本生化学会中部支部例会・シンポジウム
    • Place of Presentation
      名古屋
    • Year and Date
      2007-05-19
    • Related Report
      2008 Final Research Report
  • [Presentation] タバコ葉緑体のSD様配列のないmRNAの翻訳開始に必要なシス領域とトランス因子の解析2007

    • Author(s)
      黒田洋詩、杉浦昌弘
    • Organizer
      第71回日本生化学会中部支部例会・シンポジウム
    • Place of Presentation
      名古屋
    • Year and Date
      2007-05-19
    • Related Report
      2008 Final Research Report
  • [Presentation] 葉緑体のpsbCmRNAの翻訳は上流のAUGではなく下流のGUGから始まる2007

    • Author(s)
      黒田洋詩、鈴木晴香、久寿米木幸寛、廣瀬哲郎、湯川泰、杉浦昌弘
    • Organizer
      第30回日本分子生物学会年会・第80回日本生化学会大会合同大会
    • Place of Presentation
      横浜
    • Related Report
      2008 Final Research Report
  • [Presentation] SD様配列を持たない葉緑体mRNAの翻訳開始機構の解析2007

    • Author(s)
      黒田洋詩、杉浦昌弘
    • Organizer
      第30回日本分子生物学会年会・第80回日本生化学会大会合同大会
    • Place of Presentation
      横浜
    • Related Report
      2008 Final Research Report
  • [Presentation] 葉緑体in vitro翻訳系を用いたatpE遺伝子の翻訳機構の解析2007

    • Author(s)
      鈴木晴香、黒田洋詩、杉浦昌弘、湯川泰
    • Organizer
      第30回日本分子生物学会年会・第80回日本生化学会大会合同大会
    • Place of Presentation
      横浜
    • Related Report
      2008 Final Research Report
  • [Presentation] 葉緑体同義コドン間の翻訳効率2007

    • Author(s)
      杉浦昌弘、中邨真之
    • Organizer
      日本遺伝学会第79回大会
    • Place of Presentation
      岡山
    • Related Report
      2008 Final Research Report
  • [Presentation] Mechanisms of protein synthesis in higher plant chloroplasts2007

    • Author(s)
      Sugiura, M
    • Organizer
      10^<th> International Colloquium on Endocytobiology and Symbiosis, Gmunden, Austria
    • Place of Presentation
      invited lecture
    • Related Report
      2008 Final Research Report
  • [Presentation] 光化学系IIサブユニットCP43をコードするタバコ葉緑体psbCの翻訳開始点はGUGである2007

    • Author(s)
      黒田洋詩、鈴木晴香、久寿米木幸寛、廣瀬哲郎、湯川泰、杉浦昌弘
    • Organizer
      日本光合成研究会・公開シンポジウム
    • Place of Presentation
      岡山
    • Related Report
      2008 Final Research Report
  • [Presentation] Analysis of translation efficiencies between synonymous codons in tobacco chloroplasts with the aid of in vitro translation system

    • Author(s)
      Nakamura, M. and M. Sugiura
    • Place of Presentation
      invited lecture
    • Related Report
      2008 Final Research Report
  • [Book] Springer2008

    • Author(s)
      Sugiura, M.
    • Publisher
      RNA editing in chloroplasts. In "RNA Editing"(H.U. Goringer, ed)Springer
    • Related Report
      2008 Final Research Report
  • [Book] RNA editing in chloroplasts. In"RNA Editing"(H.U.Goringer, ed)2008

    • Author(s)
      Sugiura, M.(分担執筆)
    • Total Pages
      23
    • Publisher
      Springer-Verlag, Berlin Heidelberg
    • Related Report
      2007 Annual Research Report
  • [Remarks]

    • URL

      http://www.nsc.nagoya-cu.ac.jp/~sugiura/index.html

    • Related Report
      2008 Final Research Report
  • [Remarks]

    • URL

      http://www.nsc.nagoya-cu.ac.jp/~sugiura/index.html

    • Related Report
      2008 Annual Research Report
  • [Remarks]

    • URL

      http://www.nsc.nagoya-cu.ac.jp/~sugiura/index.html

    • Related Report
      2007 Annual Research Report

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Published: 2007-04-01   Modified: 2016-04-21  

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