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Water adaptation strategy in anuran amphibians and molecular diversity of aquaporin

Research Project

Project/Area Number 19370024
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Morphology/Structure
Research InstitutionShizuoka University

Principal Investigator

TANAKA Shigeyasu  Shizuoka University, 創造科学技術大学院, 教授 (90146233)

Co-Investigator(Kenkyū-buntansha) SUZUKI Masakazu  静岡大学, 理学部, 准教授 (60280913)
Project Period (FY) 2007 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000)
Fiscal Year 2009: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2008: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2007: ¥10,660,000 (Direct Cost: ¥8,200,000、Indirect Cost: ¥2,460,000)
Keywords水チャネル / アクアポリン / 抗利尿ホルモン / 多様性 / 水環境適応 / 無尾両生類 / バソトシン / 下腹部皮膚 / 水吸収 / 水適応 / 翻訳阻害 / 生物多様性 / 分子多様性 / アフリカツメガエル / 両生類
Research Abstract

細胞膜には,アクアポリン(AQP)と呼ばれる水を通す穴がある。カエル類は,樹上棲,陸上棲,半水棲,水棲と異なる水環境に適応し,AQP分子も多様性を示す。下腹部皮膚,膀胱,腎臓に抗利尿ホルモン依存性AQPが存在するが,系統学的な由来を異にしている。水棲ツメガエルの下腹部皮膚ではAQP mRNAは,発現しているが,タンパク質に翻訳できない仕組みがあることを発見した。これにより,ツメガエルの水中生活への適応が保障されている。

Report

(4 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • 2007 Annual Research Report
  • Research Products

    (59 results)

All 2010 2009 2008 2007 Other

All Journal Article (21 results) (of which Peer Reviewed: 20 results) Presentation (25 results) Book (10 results) Remarks (3 results)

  • [Journal Article] Water Adaptation Strategy in Anuran Amphibians: Molecular Diversity of Aquaporin.2010

    • Author(s)
      Akabane, G., Hasegawa, T., Mochida H, Matsuda M, Suzuki M, Tanaka S
    • Journal Title

      Endocrinology 151

      Pages: 165-173

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Correlation between aquaporin and water permeability in response to vasotocin, hydrin, and β-adrenergic effectors in the ventral pelvic skin of the tree frog, Hyla japonica.2010

    • Author(s)
      Ogushi Y, Kitagawa D, Hasegawa T, Suzuki M, Tanaka S
    • Journal Title

      J Exp Biol 213

      Pages: 288-294

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Water Adaptation Strategy in Anuran Amphibians : Molecular Diversity of Aquaporin2010

    • Author(s)
      Yuji Ogushi
    • Journal Title

      Endocrinology 151

      Pages: 165-173

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Correlation between aquaporin and water permeability in response to vasotocin, hydrin, and β-adrenergic effectors in the ventral pelvic skin of the tree frog, Hyla japonica2010

    • Author(s)
      Yuji Ogushi
    • Journal Title

      J Exp Biol 213

      Pages: 288-294

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Molecular and cellular regulation of water homeostasis in anuran amphibians by aquaporins.2009

    • Author(s)
      Suzuki M, Tanaka S
    • Journal Title

      Comp Biochem Physiol A 153

      Pages: 231-241

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] The spatial and temporal expression of detal-like protein 1 (Dlk1) in the rat pituitary gland during development.2009

    • Author(s)
      Nakakura T, Sato M, Suzuki M, Hatano O, Takemori H, Taniguchi Y, Minoshima Y, Tanaka S
    • Journal Title

      Histochemistry and Cell Biology 131

      Pages: 141-153

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Neuroendocrine regulation of TSH secretion in amphibians.2009

    • Author(s)
      Okada R, Kobayashi T, Yamamoto K, Nakakura T, Tanaka S, Vaudry H, Kikuyama S
    • Journal Title

      Ann NY Acad Sci 1163

      Pages: 162-170

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Demonstration of the proopiomelanocortin signaling system in the primary immune organ of the quail.2009

    • Author(s)
      Kobayashi T, Takaso A, Tanaka S, Amano M, Takahashi A, Iwamuro S, Machida T
    • Journal Title

      Ann NY Acad Sci 1163

      Pages: 441-443

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Molecular and cellular regulat ion of water homeostasis in anuran amphibians by aquaporins.2009

    • Author(s)
      Suzuki M
    • Journal Title

      Comp Biochem Physiol A. (In press)

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The spatial and temporal expression of detal-like protein 1 (Dlkl)in the rat pituitary gland during development.2009

    • Author(s)
      Nakakura T
    • Journal Title

      Histochemistry and Cell Biology 131

      Pages: 141-153

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Immunolocalization of a mammalian aquaporin 3 homologoue in water-transporting epithelial cells in several organs of the clawed toad Xenopus laevis.2008

    • Author(s)
      Mochida H, Nakakura T, Suzuki M, Hayshi H, Kikuyama S, Tanaka S
    • Journal Title

      Cell Tissue Res 333

      Pages: 297-309

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Immunolocalization of a mammalian aquaporin 3 homologoue in water-transporting epithelial cells in several organs of the clawed toad Xenopus laevis.2008

    • Author(s)
      Mochida H
    • Journal Title

      Cell Tissue Res 333

      Pages: 297-309

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Immunocytochemical and phylogenetic analyses of an arginine vasotocin-dependent aquaporin, AQP-h2K, specifically expressed in the kidney of the tree frog, Hyla japonica.2007

    • Author(s)
      Ogushi Y, Mochida H, Nakakura T, Suzuki M, Tanaka S
    • Journal Title

      Endocrinology 148

      Pages: 5891-5901

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Possible involvement of brain-derived neurotrophic (BDNF) in the innervation of dopaminergic neurons from the periventricular nucleus to the rat pars intermedia.2007

    • Author(s)
      Nakakura T, Suzuki M, Watanabe Y, Tanaka S
    • Journal Title

      Zool Sci 24

      Pages: 1086-1093

    • Related Report
      2009 Final Research Report
  • [Journal Article] Functional analysis of Nkx2. 1 and Pax9 for calcitonin gene transcription.2007

    • Author(s)
      Suzuki M, Katagiri N, Ueda M, Tanaka S
    • Journal Title

      Gen Comp Endocrinol 152

      Pages: 259-266

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Gene cloning and expression of an aquaporin (AQP-h3BL) in the basolateral membrane of water-permeable epithelial cells in osmoregulatory organs of the tree frog.2007

    • Author(s)
      Akabane G, Ogushi Y, Hasegawa T, Suzuki M, Tanaka S
    • Journal Title

      Am J Physiol Regul Integr Comp Physiol 292

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Amphibian aquaporins and adaptation to terrestrial environments: A review.2007

    • Author(s)
      Suzuki M, Hasegawa T, Ogushi Y, Tanaka S
    • Journal Title

      Comp Biochem Physiol A Mol Integr Physiol 148

      Pages: 72-81

    • Related Report
      2009 Final Research Report 2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Immunocytochemical and phylogenetic analyses of an arginine vasotocin-dependent aquaporin, AQP-h2K, specifically expressed in the kidney of the tree frog, Hyla japonica2007

    • Author(s)
      Ogushi Y, Mochida H, Nakakura T, Suzuki M, Tanaka S
    • Journal Title

      Endocrinology 148

      Pages: 5891-5901

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Gene cloning and expression of an aquaporin (AQP-h3BL)in the basolateral membrane of water-permeable epithelial cells in osmoregulatory organs of the tree frog.2007

    • Author(s)
      Akabane G, Ogushi Y, Hasegawa T, Suzuki M, Tanaka S
    • Journal Title

      Am J Physiol Regul Integr Comp Physiol 292

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Molecular diversity of vasotocin-dependent aquaporins closely associated with water adaptation strategy in anuran amphibians.

    • Author(s)
      Suzuki, M, Tanaka S
    • Journal Title

      J Neuroendocrinol (In press)

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Molecular diversity of vasotocin-dependent aquaporins closely associated with water adaptation strategy in anuran amphibians

    • Author(s)
      Masakazu Suzuki
    • Journal Title

      J Neuroendocrinol (In press)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] 両生類におけるアクアポリンの多様性とAVT応答性の進化2009

    • Author(s)
      鈴木雅一, 尾串雄次, 田中滋康
    • Organizer
      第19回バゾプレシン研究会
    • Place of Presentation
      東京
    • Year and Date
      2009-01-10
    • Related Report
      2009 Final Research Report
  • [Presentation] ネッタイツメガエルにおけるアクアポリン遺伝子の発現調節2009

    • Author(s)
      佐野貴太, 鈴木雅一, 田中滋康
    • Organizer
      第34回日本比較内分泌学会大会・日本比較生理生化学会第31回大会合同大会CompBiol2009
    • Place of Presentation
      豊中
    • Related Report
      2009 Final Research Report
  • [Presentation] 半陸上棲無尾両生類のPelvic patchにおける皮膚型アクアポリンの局在2009

    • Author(s)
      尾串雄次, 都築亜純, 鈴木雅一, 田中滋康
    • Organizer
      第34回日本比較内分泌学会大会・日本比較生理生化学会第31回大会合同大会CompBiol2009
    • Place of Presentation
      豊中
    • Related Report
      2009 Final Research Report
  • [Presentation] ニホンアマガエル下垂体における哺乳類アクアポリンに相同なAQP-h3BLの発現2009

    • Author(s)
      尾串雄次, 中倉敬, 赤羽根弦, 佐藤恵, 持田弘, 鈴木雅一, 田中滋康
    • Organizer
      (社)日本動物学会第80回大会予稿集
    • Place of Presentation
      静岡
    • Related Report
      2009 Final Research Report
  • [Presentation] 砂漠に生息するヒキガエル下腹部皮膚に発現するアクアポリン2009

    • Author(s)
      柴田侑毅, 長井孝紀, 竹内浩昭, 長谷川敬展, Stanley D Hillyard, 鈴木雅一, 田中滋康
    • Organizer
      (社)日本動物学会第80回大会予稿集
    • Place of Presentation
      静岡
    • Related Report
      2009 Final Research Report
  • [Presentation] Molecular diversity of vasotocin-dependent aquaporins closely associated with water adaptation strategy in anuran amphibians2009

    • Author(s)
      Suzuki M, Tanaka S
    • Organizer
      VIIIth World Congress on Neurohypophysial Hormones
    • Place of Presentation
      Kiatakyuushuu
    • Related Report
      2009 Final Research Report
  • [Presentation] 下垂体プロオピオメラノコルチン細胞に関する分子細胞形態学的研究2009

    • Author(s)
      田中滋康
    • Organizer
      第24回日本下垂体研究会
    • Place of Presentation
      三沢
    • Related Report
      2009 Final Research Report
  • [Presentation] Molecular diversity of vasotocin-dependent aquaporins closely associated with water adaptation strategy in anuran amphibians2009

    • Author(s)
      Masakazu Suzuki
    • Organizer
      VII^<th> World Congress on Neurohypophysial Hormones
    • Place of Presentation
      Kitakyushu
    • Related Report
      2009 Annual Research Report
  • [Presentation] カエルの水適応におけるアクアポリンの多様性とホルモン調節2008

    • Author(s)
      鈴木雅一
    • Organizer
      第33回日本比較内分泌学会大会及びシンポジウム
    • Place of Presentation
      広島
    • Year and Date
      2008-12-06
    • Related Report
      2008 Annual Research Report
  • [Presentation] アフリカツメガエルに発現する下腹部皮膚型アクアポリンの発現調節2008

    • Author(s)
      尾串雄次, 赤羽根弦, 鈴木雅一, 田中滋康
    • Organizer
      (社)日本動物学会第79回大会
    • Place of Presentation
      福岡
    • Related Report
      2009 Final Research Report 2008 Annual Research Report
  • [Presentation] カエルの水適応におるアクアポリンの多様性とホルモン調節2008

    • Author(s)
      鈴木雅一, 田中滋康
    • Organizer
      日本比較内分泌学会シンポジウム
    • Place of Presentation
      広島
    • Related Report
      2009 Final Research Report
  • [Presentation] アフリカツメガエルにおける膀胱型アクアポリン(AQP)の同定と機能解析2008

    • Author(s)
      片山いずみ, 尾串雄次, 中倉敬, 持田弘, 鈴木雅一, 田中滋康
    • Organizer
      (社)日本動物学会第79回大会
    • Place of Presentation
      福岡
    • Related Report
      2009 Final Research Report 2008 Annual Research Report
  • [Presentation] アマガエル下垂体のゴナドトロピン細胞における哺乳類アクアポリン3と相同タンパク質の局在2008

    • Author(s)
      塩田真央
    • Organizer
      第35回日本神経内分泌学会第23会日本下垂体研究会合同学術集会
    • Place of Presentation
      東京
    • Related Report
      2008 Annual Research Report
  • [Presentation] アマガエル下腹部皮膚の水吸収の仕組み2008

    • Author(s)
      塩田真央
    • Organizer
      (社)日本動物学会第79回大会
    • Place of Presentation
      福岡
    • Related Report
      2008 Annual Research Report
  • [Presentation] Timing of the frog's jump out of a pond anuran amphibian aquaporin water channels2007

    • Author(s)
      Tanaka S, Ogushi Y, Suzuki M
    • Organizer
      Comprehensive understanding of diverse biological timing mechanism.
    • Place of Presentation
      Okayama
    • Related Report
      2009 Final Research Report
  • [Presentation] 無尾両生類の下腹部皮膚に発現するAVT依存性AQPの多様性2007

    • Author(s)
      尾串雄次, 赤羽根弦, 鈴木雅一, 田中滋康
    • Organizer
      第32回日本比較内分泌学会大会およびシンポジウム
    • Place of Presentation
      日光
    • Related Report
      2009 Final Research Report
  • [Presentation] ウシガエル下垂体中葉細胞に発現するアクアポリン(AQP^h3BL)タンパク質2007

    • Author(s)
      小宮山雄太, 鈴木雅一, 菊山榮, 田中滋康
    • Organizer
      第32回日本比較内分泌学会大会およびシンポジウム
    • Place of Presentation
      日光
    • Related Report
      2009 Final Research Report
  • [Presentation] 「カエルも汗をかくか?」シンポジウム:両生類はワンダーランド2007

    • Author(s)
      田中滋康
    • Organizer
      日本動物学会第78回大会
    • Place of Presentation
      弘前
    • Related Report
      2009 Final Research Report
  • [Presentation] アマガエル腎臓に特異的に発現するアクアポリンの同定および免疫組織化学的解析2007

    • Author(s)
      尾串雄次, 中倉敬, 鈴木雅一, 田中滋康
    • Organizer
      日本動物学会第78回大会
    • Place of Presentation
      弘前
    • Related Report
      2009 Final Research Report
  • [Presentation] 哺乳類アクアポリン(AQP)3に相同なツメガエルAQP-x3BLの免疫組織学的発現部位2007

    • Author(s)
      持田弘, 中倉敬, 鈴木雅一, 菊山榮, 田中滋康
    • Organizer
      日本動物学会第78回大会
    • Place of Presentation
      弘前
    • Related Report
      2009 Final Research Report
  • [Presentation] カエルには, 下垂体神経葉ホルモンバソトシン(AVT)依存性アクアポリンが3種類存在する2007

    • Author(s)
      田中滋康, 尾串雄次, 持田弘, 赤羽根弦, 中倉敬, 鈴木雅一
    • Organizer
      日本下垂体研究会第22回学術集会
    • Place of Presentation
      神奈川
    • Related Report
      2009 Final Research Report
  • [Presentation] Evolution of regulatory systems in anuran amphibian water homeostasis for adapting to terrestrial environments.2007

    • Author(s)
      Tanaka S, Suzuki M
    • Organizer
      5th International Conference of Aquaporin
    • Place of Presentation
      Nara
    • Related Report
      2009 Final Research Report
  • [Presentation] Expression of an aquaporin, AQP-h3BL, in the basolateral membrane of water-permeable epithelial cells in osmoregulatory organs of the tree frog.2007

    • Author(s)
      Akabane G, Ogushi Y, Hasegawa T, Suzuki M, Tanaka S
    • Organizer
      5th International Conference of Aquaporin
    • Place of Presentation
      Nara
    • Related Report
      2009 Final Research Report
  • [Presentation] Immunocytochemical and phylogenetic analyses of an arginine vatotocin-dependent aquaporin, AQP-h2K, specifically expressed in the kidney of the tree frog.2007

    • Author(s)
      Ogushi Y, Mochida H, Akabane G, Suzuki M, Tanaka S
    • Organizer
      5th International Conference of Aquaporin
    • Place of Presentation
      Nara
    • Related Report
      2009 Final Research Report
  • [Presentation] Evolution of regulatory systems in anuran amphibian water homeostasis for adapting to terrestrial environments2007

    • Author(s)
      Tanaka S
    • Organizer
      The 5th International Conference of Aquaporin:Explorig new functions of aquaporin
    • Place of Presentation
      Nara
    • Related Report
      2007 Annual Research Report
  • [Book] Synthetic and Integrative Biology2010

    • Author(s)
      Shigeyasu Tanaka
    • Publisher
      Nova Science Publishers(In press)
    • Related Report
      2009 Annual Research Report
  • [Book] Integrative Biology of Aquaporins in Anuran Amphibians2009

    • Author(s)
      Tanaka S, Suzuki M
    • Publisher
      Nova Science Publishers(in press)
    • Related Report
      2009 Final Research Report
  • [Book] Osmotic and ion regulation in amphibians2009

    • Author(s)
      Hillyard DS, Mobjerg N, Tanaka S, Larsen EH
    • Publisher
      CRC press
    • Related Report
      2009 Final Research Report
  • [Book] Osmotic and ion regulation in amphibians. In : Osmotic and Ionic Regulation : Cells and Animals2009

    • Author(s)
      Hillyard D
    • Publisher
      CRC press
    • Related Report
      2008 Annual Research Report
  • [Book] 両生類の水代謝 In : 水とからだの事典2009

    • Author(s)
      田中滋康
    • Publisher
      朝倉書店
    • Related Report
      2008 Annual Research Report
  • [Book] 両生類の水代謝(佐々木成,石橋賢一編集)2008

    • Author(s)
      田中滋康
    • Publisher
      朝倉書店
    • Related Report
      2009 Final Research Report
  • [Book] 高感度mRNA検出法で細胞を同定する-in situ RT-PCR法-比較生理化学2007

    • Author(s)
      中倉敬,田中滋康
    • Related Report
      2009 Final Research Report
  • [Book] 「動物のアクアポリンの多様性と比較「内分泌的展望」特集新しい内分泌現象2007

    • Author(s)
      田中滋康,長谷川敬展,鈴木雅一
    • Publisher
      日本比較内分泌学会
    • Related Report
      2009 Final Research Report
  • [Book]

    • Author(s)
      田中滋康生物学辞典
    • Publisher
      東京化学同人(印刷中)
    • Related Report
      2009 Final Research Report
  • [Book] 水とからだの事典(分担:両生類の水代謝)

    • Author(s)
      佐々木 成, 石橋 賢一編集
    • Publisher
      朝倉書店
    • Related Report
      2007 Annual Research Report
  • [Remarks]

    • URL

      http://www.ipc.shizuoka.ac.jp/~sbstana/ST-Lab-J.html

    • Related Report
      2009 Final Research Report
  • [Remarks]

    • URL

      http://www.ipc.shizuoka.ac.jp/sbstana/ST-Lab-J.html

    • Related Report
      2008 Annual Research Report
  • [Remarks]

    • URL

      http://www.ipc.shizuoka.ac.jp/~sbstana/ST-Lab-J.html

    • Related Report
      2007 Annual Research Report

URL: 

Published: 2007-04-01   Modified: 2016-04-21  

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