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How does firefly luciferase control the bioluminescence reaction?

Research Project

Project/Area Number 19370039
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Structural biochemistry
Research InstitutionKyoto University

Principal Investigator

NAKATSU Toru  Kyoto University, 薬学研究科, 准教授 (50293949)

Co-Investigator(Kenkyū-buntansha) HIRATAKE Jun  京都大学, 化学研究所, 教授 (80199075)
KATO Taiichirou  兵庫県立大学, 工学研究科, 助教 (60423901)
KIMURA Yasuhisa  京都大学, 農学研究科, 助教 (10415143)
Project Period (FY) 2007 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
Fiscal Year 2009: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2008: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2007: ¥10,790,000 (Direct Cost: ¥8,300,000、Indirect Cost: ¥2,490,000)
Keywords構造生物学 / ルシフェラーゼ / 発光反応 / X線結晶構造解析 / 量子収率 / ルシフェリン / オキシルシフェリン / ルシフェリン再生酵素 / 反応中間体アナログ / 遷移状態アナログ / 結晶化 / 変異体
Research Abstract

We measure the bioluminescent activities and determine the crystal structures of G326S and Y257A firefly luciferase mutants which emit red light. In the result, it is supposed that the emission color change from yellow-green to red and the decrease of the emission are caused by the energy loss of the excited oxyluciferin. In addition we determine the crystal structure of luciferin regenerating enzyme, which controls the emission reaction in firefly.

Report

(4 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • 2007 Annual Research Report
  • Research Products

    (23 results)

All 2010 2009 2008 2007

All Journal Article (14 results) (of which Peer Reviewed: 13 results) Presentation (9 results)

  • [Journal Article] Deleting two C-terminal alpha-helices is effective to crystallize the bacterial ABC transporter Escherichia coli MsbA complexed with AMP-PNP2010

    • Author(s)
      Terakado K, Kodan A, Nakano H, Kimura Y, Ueda K, Nakatsu T, Kato H.
    • Journal Title

      Acta Crystallogr D Vol.66

      Pages: 319-323

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] 高機能化ホタル・ルシフェラーゼ創製に向けて2010

    • Author(s)
      中津亨
    • Journal Title

      日本結晶学会誌 52(1)

      Pages: 81-88

    • NAID

      10026107980

    • Related Report
      2009 Final Research Report
  • [Journal Article] Deleting two C-terminal alpha-helices is effective to crystallize the bacterial ABC transporter Escherichia coli MsbA complexed with AMP-PNP2010

    • Author(s)
      Terakado K, Kodan A, Nakano H, Kimura Y, Ueda K, Nakatsu T, Kato H.
    • Journal Title

      Acta Crystallogr D 66

      Pages: 319-323

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] X-ray crystallographic study of an HIV-1 fusion inhibitor with the gp41 S138A substitution.2009

    • Author(s)
      Watabe T, Terakawa Y, Watanabe K, Ohno H, Nakano H, Nakatsu T, Kato H, Izumi K, Kodama E, Matsuoka M, Kitaura K, Oishi S, Fujii N.
    • Journal Title

      J Mol Biol. Vol.392

      Pages: 657-665

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Functional role of the linker region in purified human P-glycoprotein.2009

    • Author(s)
      Sato T, Kodan A, Kimura Y, Ueda K, Nakatsu T, Kato H.
    • Journal Title

      FEBS J. Vol.276

      Pages: 3504-3516

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] X-ray crystallographic study of an HIV-1 fusion inhibitor with the gp41 S138A substitutio2009

    • Author(s)
      Watabe T, Terakawa Y, Watanabe K, Ohno H, Nakano H, Nakatsu T, Kato H, Izumi K, Kodama E, Matsuoka M, Kitaura K, Oishi S, Fujii N.
    • Journal Title

      J Mol Biol. 392

      Pages: 657-665

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Functional role of the linker region in purified human P-glycoprotein2009

    • Author(s)
      Sato T, Kodan A, Kimura Y, Ueda K, Nakatsu T, Kato H.
    • Journal Title

      FEBS J. 276

      Pages: 3504-3516

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Structural basis for gibberellin recognition by its receptor GID12008

    • Author(s)
      Shimada A, Ueguchi-Tanaka M, Nakatsu T, Nakajima M, Naoe Y, Ohmiya H, Kato H, Matsuoka M.
    • Journal Title

      Nature Vol.456

      Pages: 520-523

    • NAID

      130006990929

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Mechanism of efficient firefly bioluminescence via adiabatic transition state and seam of sloped conical intersection2008

    • Author(s)
      Chung LW, Hayashi S, Lundberg M, Nakatsu T, Kato H, Morokuma K.
    • Journal Title

      J Am Chem Soc. Vol.130

      Pages: 12880-12881

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] A direct repeat of E-box-like elements is required for cell-autonomous circadian rhythm of clock genes2008

    • Author(s)
      Nakahata Y, Yoshida M, Takano A, Soma H, Yamamoto T, Yasuda A, Nakatsu T, Takumi T.
    • Journal Title

      BMC Mol Biol. 1

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Structural basis for gibberellin recognition by its receptor GID12008

    • Author(s)
      Shimada A, Ueguchi-Tanaka M, Nakatsu T, Nakajima M, Naoe Y, Ohmiya H, Kato H, Matsuoka M.
    • Journal Title

      Nature 456

      Pages: 520-523

    • NAID

      130006990929

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mechanism of efficient firefly bioluminescence via adiabatic transition state and seam of sloped conical intersection2008

    • Author(s)
      Chung LW, Hayashi S, Lundberg M, Nakatsu T, Kato H, Morokuma K.
    • Journal Title

      J Am Chem Soc. 130

      Pages: 12880-12881

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A direct repeat of E-box-like elements is required for cell-autonomous circadian rhythm of clock genes2008

    • Author(s)
      Nakahata Y, Yoshida M, Takano A, Soma H, Yamamoto T, Yasuda A,
    • Journal Title

      BMC Mol Biol. 0:00

      Pages: 1-1

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Crystallization and preliminary X-ray crystallographic studies of Pz peptidase A from Geobacillus2007

    • Author(s)
      Kawasaki, A., Nakano, H., Tsujimoto, Y., Matsui, H.,
    • Journal Title

      Acta Crystallogr. F63

      Pages: 142-144

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] ホタル・ルシフェラーセによるπ空間制御機構2009

    • Author(s)
      寺角香菜子、中津亨
    • Organizer
      日本生物物理学会
    • Place of Presentation
      アスティ徳島
    • Year and Date
      2009-10-30
    • Related Report
      2009 Final Research Report
  • [Presentation] ホタル・ルシフェラーゼによるπ空間制御機構2009

    • Author(s)
      寺角香菜子, 中津亨
    • Organizer
      日本生物物理学会
    • Place of Presentation
      アスティ徳島
    • Year and Date
      2009-10-30
    • Related Report
      2009 Annual Research Report
  • [Presentation] ヒト由来ペルオキシソーム膜タンパク質輸送因子Pex3pとPex19pの構造基盤2009

    • Author(s)
      佐藤康彦, 柴田洋之, 中津亨, 中野博明, 柏山恭範, 今中常雄, 加藤博章
    • Organizer
      日本生化学会
    • Place of Presentation
      神戸ポートアイランド
    • Year and Date
      2009-10-23
    • Related Report
      2009 Annual Research Report
  • [Presentation] メタノール資化性酵母 Pichia pastoris 由来Pex3-Pex19p相互作用の解析2009

    • Author(s)
      平井秀憲, 江川響子, 柴田洋之, 中津亨, 加藤博章
    • Organizer
      日本生化学会
    • Place of Presentation
      神戸ポートアイランド
    • Year and Date
      2009-10-23
    • Related Report
      2009 Annual Research Report
  • [Presentation] ホタルルシフェラーゼは基質の不斉をどのように見分けているのか?2009

    • Author(s)
      加藤太一郎, 宮永佳奈, 番匠亜沙美, 中津亨, 武尾正弘, 根来誠司
    • Organizer
      日本生物工学会
    • Place of Presentation
      名古屋大学
    • Year and Date
      2009-09-24
    • Related Report
      2009 Annual Research Report 2009 Final Research Report
  • [Presentation] ジベレリン受容体の立体構造に基づく分子進化と基質認識機構の解明2009

    • Author(s)
      島田麻子, 上口(田中)美弥子, 中津亨, 中嶋正敏, 直江洋一, 大宮博子, 加藤博章, 松岡信
    • Organizer
      蛋白質科学会
    • Place of Presentation
      熊本全日空ホテルニュースカイ
    • Year and Date
      2009-05-20
    • Related Report
      2009 Annual Research Report
  • [Presentation] ホタルにおける発光制御メカニズム2009

    • Author(s)
      中津亨
    • Organizer
      日本農芸化学会2009年度大会
    • Place of Presentation
      福岡国際会議場
    • Year and Date
      2009-03-28
    • Related Report
      2009 Final Research Report
  • [Presentation] ルシフェリン再生酵素のX線結晶構造解析2009

    • Author(s)
      奥田智彦、五味恵子、梶山直樹、加藤博章、中津亨
    • Organizer
      日本薬学会
    • Place of Presentation
      国立京都国際会館
    • Year and Date
      2009-03-26
    • Related Report
      2009 Final Research Report
  • [Presentation] ゲンジボタル・ルシフェラーゼの発光反応および発光色制御機構の構造基盤2008

    • Author(s)
      中津亨
    • Organizer
      酵素工学研究会
    • Place of Presentation
      京都テルサ
    • Year and Date
      2008-04-25
    • Related Report
      2009 Final Research Report

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Published: 2007-04-01   Modified: 2016-04-21  

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