Proteomic analysis of EGF receptor-mediated signaling

• Project/Area Number: 19370041
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Structural biochemistry
• Research Institution: The University of Tokushima
• Principal Investigator: TANIGUCHI Hisaaki The University of Tokushima, 疾患酵素学研究センター, 教授 (10257636)
• Co-Investigator(Kenkyū-buntansha): KONISHI Hiroaki 徳島大学, 疾患酵素学研究センター, 准教授 (40252811) ; FUJIWARA Kazuko 徳島大学, 疾患酵素学研究センター, 准教授 (20108880) ; IKEDA Kazuko 徳島大学, 疾患酵素学研究センター, 助教 (10108863) ; KOSAKO Hidetaka 徳島大学, 疾患酵素学研究センター, 准教授 (10291171)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000); Fiscal Year 2009: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2008: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2007: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
• Keywords: タンパク質 / プロテオーム / バイオテクノロジー / シグナル伝達 / 分析科学 / 蛋白質

Research Abstract

Four novel proteins identified by the phosphoproteomic analysis of the EGF receptor mediated signaling pathways were studied for their physiological functions. The proteins include Ymer protein, CFBP, CLPABP (Caldiolipine and Phosphatidic Acid Binding Protein) and GAREM (Grb2-associated and regulator of Erk/MAPK). The posttranslational modifications such as phosphorylation and ubiquitination were analyzed for their regulatory roles in the EGF receptor signaling.

Research Products

• [Journal Article] Phosphoproteomics reveals new ERK MAP kinase targets and links ERK to nucleoporin-mediated nuclear transport. (2009)
  • Author(s): Kosako, H., Yamaguchi, N., Aranami, C., Ushiyama, M., Kose, S., Imamoto, N., Taniguchi, H., Nishida, E., Hattori, S.
  • Journal Title: Nature Struct. Mol. Biol. 16 Pages: 1026-1035
  • Peer Reviewed
• [Journal Article] Mitotic regulation of the stability of microtubule plus-end tracking protein EB3 by ubiquitin ligase SIAH-1 and Aurora mitotic kinases (2009)
  • Author(s): Ban, R., Matsuzaki, H., Akashi, T., Sakashita, G., Taniguchi, H., Park, S, Y. Tanaka, H., Furukawa, K., Urano, T.
  • Journal Title: J. Biol. Chem. 284 Pages: 28367-28381
  • Peer Reviewed
• [Journal Article] a novel adaptor protein for growth factor receptor-bound protein 2, contributes to cellular transformation through the activation of extracellular signal-regulated kinase signaling. (2009)
  • Author(s): Tashiro K, Tsunematsu T, Okubo H, Ohta T, Sano E, Yamauchi E, Taniguchi H, Konishi H. GAREM
  • Journal Title: J. Biol. Chem. 284 Pages: 20206-20214
  • Peer Reviewed
• [Journal Article] Comprehensive egg-coat proteome of an ascidian Ciona intestinalis reveals gamete recognition molecules involved in self-sterility. (2009)
  • Author(s): Yamada, L., Saito, T., Taniguchi, H., Sawada, H., Harada, Y.
  • Journal Title: J. Biol. Chem. 284 Pages: 9402-9410
  • Peer Reviewed
• [Journal Article] Comprehensive egg-coat proteome of an ascidian Ciona intestinalis reveals gamete recognition molecules involved in self-sterility. (2009)
  • Author(s): Yamada, L.
  • Journal Title: J.Biol.Chem., 284 Pages: 9402-9410
  • Peer Reviewed
• [Journal Article] GAREM, a novel adaptor protein for growth factor receptor-bound protein 2, contributes to cellular transformation through the activation of extracellular signal-regulated kinase signaling. (2009)
  • Author(s): Tashiro K.
  • Journal Title: J.Biol.Chem. 284 Pages: 20206-20214
  • Peer Reviewed
• [Journal Article] Mitotic regulation of the stability of microtubule plus-end tracking protein EB3 by ubiquitin ligase SIAH-1 and Aurora mitotic kinases (2009)
  • Author(s): Ban, R.
  • Journal Title: J.Biol.Chem 284 Pages: 28367-28381
  • Peer Reviewed
• [Journal Article] Phosphoproteomics reveals new ERK MAP kinase targets and links ERK to nucleoporin-mediated nuclear transport. (2009)
  • Author(s): Kosako, H
  • Journal Title: Nature Struct.Mol.Biol. 16 Pages: 1026-1035
  • Peer Reviewed
• [Journal Article] An a posteriori calibration method for improving protein identification accuracy in proteomics using electrospray ionization time-of-flight tandem mass spectrometry. (2008)
  • Author(s): Ishino Y., Taniguchi, H
  • Journal Title: Rapid Commun. Mass Spectrom. 22 Pages: 1335-1338
  • Peer Reviewed
• [Journal Article] Contribution of peroxisome-specific isoform of Lon protease in sorting PTS1 proteins to peroxisomes. (2008)
  • Author(s): Omi, S., Nakata, R., Okamura-Ikeda, K., Konishi, H., Taniguchi, H.
  • Journal Title: J. Biochem 143 Pages: 649-660
  • NAID: 10024909907
  • Peer Reviewed
• [Journal Article] Mechanism of Self-Sterility in a Hermaphroditic Chordate. (2008)
  • Author(s): Harada, Y., Takagaki, Y., Sunagawa, M., Saito, T., Yamada, L., Taniguchi, H., Shoguchi, E., Sawada, H.
  • Journal Title: Science 320 Pages: 548-550
  • Peer Reviewed
• [Journal Article] Novel tyrosine phosphorylated and cardiolipin-binding protein CLPABP functions as mitochondrial RNA granule. (2008)
  • Author(s): Sano, E., Shono, S., Tashiro, K., Konishi, H., Yamauchi, E., Taniguchi, H.
  • Journal Title: Biochim Biophys Acta Mol. Cell. Res. 1783 Pages: 1036-1047
  • Peer Reviewed
• [Journal Article] An a posteriori calibration method for improving protein identification accuracy in proteomics using electrospray ionization time-of-flight tandem mass spectrometry. (2008)
  • Author(s): Ishino Y.
  • Journal Title: Rapid Commun Mass Spectrom. 22 Pages: 1335-1338
  • Peer Reviewed
• [Journal Article] Mechanism of self-sterility in a hermaphroditic chordate. (2008)
  • Author(s): Harada Y.
  • Journal Title: Science. 320 Pages: 548-550
  • Peer Reviewed
• [Journal Article] Contribution of Peroxisome-specific Isoform of Lon Protease in Sorting PTS1 Proteins to Peroxisomes. (2008)
  • Author(s): Omi s.
  • Journal Title: J Biochem. 143 Pages: 649-660
  • Peer Reviewed
• [Journal Article] Novel tyrosine phosphorylated and cardiolipin-binding protein CLPABP functions as mitochondrial RNA granule. (2008)
  • Author(s): Sano E.
  • Journal Title: Biochim Biophys Acta. Mol. Cell. Res. 1783 Pages: 1036-1047
  • Peer Reviewed
• [Journal Article] Mass spectrometry-based prokaryote gene annotation. (2007)
  • Author(s): Ishino, Y., Okada, H., Ikeuchi, M., Taniguchi, H.
  • Journal Title: Proteomics 22 Pages: 4053-4065
  • Peer Reviewed
• [Journal Article] Crystal Structure of Bovine Lipoyltransferase in Complex with Lipoyl-AMP. (2007)
  • Author(s): Fujiwara, K., Hosaka, H., Matsuda, M., Okamura-Ikeda, K., Motokawa, Y., Suzuki, M., Nakagawa, A., Taniguchi, H.
  • Journal Title: J Mol Biol 371 Pages: 222-234
  • Peer Reviewed
• [Journal Article] PGC7/Stella protects against DNA demethylation in early embryogenesis. (2007)
  • Author(s): Nakamura, T., Arai, Y., Umehara, H., Masuhara, M., Kimura, T., Taniguchi, H., Sekimoto, T., Ikawa, M., Yoneda, Y., Okabe, M., Tanaka, S., Shiota, K., Nakano, T.
  • Journal Title: ature Cell Biology 9 Pages: 64-71
  • Peer Reviewed
• [Journal Article] タンパク質間相互作用におけるアシル化の役割 (2007)
  • Author(s): 谷口寿
  • Journal Title: 生体の科学 58(5) Pages: 360-363
• [Journal Article] Crystal structure of bovine lipoyltransferase in complex with lipoyl-AMP (2007)
  • Author(s): Fujiwara K.
  • Journal Title: J Mol Biol 371 Pages: 222-234
  • Peer Reviewed
• [Journal Article] PGC7/Stella protects against DNA demethylation in early embryogenesis (2007)
  • Author(s): Nakamura T.
  • Journal Title: Nat Cell Biol 9 Pages: 64-71
  • Peer Reviewed
• [Journal Article] Mass spectrometry based prokaryote gene annotation (2007)
  • Author(s): Ishino Y.
  • Journal Title: Proteomics 22 Pages: 4053-4065
  • Peer Reviewed
• [Presentation] Western blot-like presentation of gel-enhanced LC/MS data and software-based detection of protein modifications (2010)
  • Author(s): 吉沢明康
  • Organizer: RECOMBサテライトミーティング
  • Place of Presentation: アトキンソンホール、UCSD、サンディエゴ、USA
  • Year and Date: 2010-03-28
• [Presentation] Annotation of prokaryote genomes using large-scale proteomic data (2010)
  • Author(s): 谷口寿章
  • Organizer: RECOMBサテライトミーティング
  • Place of Presentation: アトキンソンホール、UCSD、サンディエゴ、USA
  • Year and Date: 2010-03-27
• [Presentation] ProteoFiT : a bundle of VBA macros for parsing, filtering and analyzing Mascot database search results for large-scale proteomics. (2010)
  • Author(s): 谷口貴子
  • Organizer: RECOMBサテライトミーティング
  • Place of Presentation: アトキンソンホール、UCSD、サンディエゴ、USA
  • Year and Date: 2010-03-27
• [Presentation] a bundle of VBA macros for parsing, filtering and analyzing Mascot database search results for large-scale proteomics. (2010)
  • Author(s): 谷口貴子、谷口寿章ProteoFiT
  • Organizer: RECOM Satellite Meeting on Computational Proteomics.
  • Place of Presentation: UCSD, San Diego, USA
• [Presentation] Annotation of prokaryote genomes using large-scale proteomic data. (2010)
  • Author(s): 谷口寿章
  • Organizer: RECOM Satellite Meeting on Computational Proteomics
  • Place of Presentation: UCSD, San Diego, USA
• [Presentation] Western blot-like presentation of gel-enhanced LC/MS data and software-based detection of protein modifications. (2010)
  • Author(s): 吉沢明康、山田力志、谷口寿章
  • Organizer: RECOM Satellite Meeting on Computational Proteomics.
  • Place of Presentation: UCSD, San Diego, USA
• [Presentation] Phosphorylation of nuclear pore complex proteins by ERK MAP kinase regulates interaction with transport receptors. (2010)
  • Author(s): 小迫英尊
  • Organizer: ASCB 49th Annual Meeting
  • Place of Presentation: San Diego, USA
• [Presentation] Phosphorylation of nuclear pore complex proteins by ERK MAP kinase regulates interaction with transport receotors. (2009)
  • Author(s): 小迫英尊
  • Organizer: 2009 ASCB Annual Meeting
  • Place of Presentation: コンベンションセンター(サンディェゴ)
  • Year and Date: 2009-12-06
• [Presentation] Ascidian Adult Body Map: A proteomic view of adult ascidian tissues. (2009)
  • Author(s): 吉沢明康、山田力志、谷口寿章
  • Organizer: The 5th International Tunicate Meeting
  • Place of Presentation: Naha, Okinawa
  • Year and Date: 2009-06-22
• [Presentation] Global protein profiling of ascidian C. intestinalis: toward the comprehensive understanding at the protein level (2009)
  • Author(s): 山田力志、谷口寿章
  • Organizer: The 5th International Tunicate Meeting
  • Place of Presentation: Naha, Okinawa
  • Year and Date: 2009-06-22
• [Presentation] Global protein profiling of ascidian C.intestinalis : toward the comprehensive understanding at the protein level (2009)
  • Author(s): 山田力志
  • Organizer: The 5^<th> International Tunicate Meeting
  • Place of Presentation: 沖縄産業支援センター
  • Year and Date: 2009-06-22
• [Presentation] Ascidian Adult Body Map : A proteomic view of adult ascidian tissues (2009)
  • Author(s): 吉沢明康
  • Organizer: The 5^<th> International Tunicate Meeting
  • Place of Presentation: 沖縄産業支援センター
  • Year and Date: 2009-06-22
• [Presentation] プロテオミクスによる細胞生物学へのアプローチ日本細胞生物学会大会 (2009)
  • Author(s): 谷口寿章
  • Place of Presentation: 名古屋
  • Year and Date: 2009-06-04
• [Presentation] プロテオミクスによる細胞生物学へのアプローチ (2009)
  • Author(s): 谷口寿章
  • Organizer: 日本細胞生物学会大会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2009-06-04
• [Presentation] Ascidian Adult Body Map:質量分析によるホヤ・プロテオーム・データベースの構築、日本分子生物学会 (2009)
  • Author(s): 吉沢明康、山田力志、谷口寿章
  • Organizer: 第9回春季シンポジウム
  • Place of Presentation: 宮崎
  • Year and Date: 2009-05-11
• [Presentation] Ascidian Adult Body Map : 質量分析によるホヤ・プロテオーム・データベースの構築 (2009)
  • Author(s): 吉沢明康
  • Organizer: 日本分子生物学会 第9回春季シンポジウム
  • Place of Presentation: シーガイヤワールドコンベンションセンター・サミット
  • Year and Date: 2009-05-11
• [Presentation] プロテオミクスによるリンパ腫特異的血清マーカーの探索BMB2008 (2008)
  • Author(s): 庄野繁一、谷口貴子、木戸慎介、阿倍正博、松本俊夫、谷口寿章
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-12
• [Presentation] プロテオミクスによるリンパ腫特異的血清マーカーの探索 (2008)
  • Author(s): 庄野 繁一
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会・合同大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2008-12-12
• [Presentation] グリシン開裂酵素系T-Hタンパク質複合体の結晶構造解析と反応機構BMB2008 (2008)
  • Author(s): 池田和子, 保坂晴美, 真板宣夫, 藤原和子, 中川敦史, 本川雄太郎, 谷口寿章
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-09
• [Presentation] 大腸菌lipoate-protein ligase Aによる2段階反応の構造的基盤BMB2008 (2008)
  • Author(s): 藤原和子, 保坂晴美, 池田和子, 真板宣夫, 本川雄太郎, 中川敦史, 谷口寿章
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-09
• [Presentation] 大腸菌lipoate-protein ligase Aによる2段階反応の構造的基盤 (2008)
  • Author(s): 藤原 和子
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会・合同大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2008-12-09
• [Presentation] グリシン開裂酵素系T-Hタンパク質複合体の結晶構造解析と反応機構 (2008)
  • Author(s): 池田 和子
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会・合同大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2008-12-09
• [Presentation] 質量分析を基盤とするプロテオミクスの医学・生物学研究への応用 (2008)
  • Author(s): 谷口寿章
  • Organizer: 第56回質量分析総合討論会
  • Place of Presentation: つくば
  • Year and Date: 2008-05-15
• [Presentation] 質量分析を基盤とするプロテオミクスの医学・生物学研究への応用 (2008)
  • Author(s): 谷口 寿章
  • Organizer: 第56回質量分析総合討論会
  • Place of Presentation: つくば国際会議場エポカル
  • Year and Date: 2008-05-15
• [Presentation] グリシン開裂酵素系T-Hタンパク質複合体の結晶構造解析 (2007)
  • Author(s): 池田 和子
  • Organizer: 第30回日本分子生物学会・第80回日本生化学会合同大会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2007-12-14
• [Presentation] X線結晶構造解析によるリポ酸転移酵素の触媒機構の解明 (2007)
  • Author(s): 藤原 和子
  • Organizer: 第30回日本分子生物学会・第80回日本生化学会合同大会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2007-12-11
• [Presentation] 脂肪細胞分泌タンパク質のプロテオーム解析による新規アディポサイトカインの探索 (2007)
  • Author(s): 奥田 祐子
  • Organizer: 第28回日本肥満学会
  • Place of Presentation: 都市センターホテル
  • Year and Date: 2007-10-20
• [Presentation] Secretome Analysis of Human Adipocytes Obtained from Visceral and Subcutaneous Adipose Tissues (2007)
  • Author(s): Yuko Okuda
  • Organizer: Hupo 6th ANNUAL WORLD CONGRESS, SEOUL 2007
  • Place of Presentation: COEX
  • Year and Date: 2007-10-09
• [Book] 「プロテオミクスと病態解析」図説分子病態学第4版 (2008)
  • Author(s): 谷口寿章, 一瀬白帝、鈴木宏治(共編)
  • Publisher: 中外医学社刊
• [Remarks]
  • URL: http://www.ier.tokushima-u.ac.jp/
• [Patent(Industrial Property Rights)] 生理活性物質と相互作用する物質の効率的スクリーニング方法 (2007)
  • Inventor(s): 谷口 寿章, 他
  • Industrial Property Rights Holder: 徳島大学, 他
  • Filing Date: 2007-08-28