Structure and function of autotransporter proteins from pathogenic bacteria

• Project/Area Number: 19370045
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Structural biochemistry
• Research Institution: Yokohama City University
• Principal Investigator: TAME Jeremy R.H. Yokohama City University, 国際総合科学研究科, 教授 (00336588)
• Co-Investigator(Kenkyū-buntansha): PARK Sam-yong 横浜市立大学, 国際総合科学研究, 准教授 (20291932) ; UNZAI Satoru 横浜市立大学, 国際総合科学研究科, 助教 (60336592)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000); Fiscal Year 2008: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000); Fiscal Year 2007: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000)
• Keywords: 構造生物学 / X線結晶構造解析 / タンパク質 / オートトランスポーター

Research Abstract

オートトランスポータータンパク質Heme Binding Protein (HBP)のパッセンジャードメイン立体構造解析に成功した。HBPの外膜貫通領域βバレルドメインとオートシャペロン部分の発現系構築に成功し、タンパク質の結晶化および立体構造解析に成功した。

Research Products

• [Journal Article] The nature of the TRAP-Anti-TRAP complex (2009)
  • Author(s): Watanabe, M., Heddle, J. G., Kikuchi, K., Unzai, S., Akashi, S., Park, S. Y. & Tame, J. R.
  • Journal Title: Proc Natl Acad Sci U S A 106 Pages: 2176-81
  • Peer Reviewed
• [Journal Article] Structural insight into the essential PB1-PB2 subunit contact of the influenza virus RNA polymerase (2009)
  • Author(s): Sugiyama, K., Obayashi, E., Kawaguchi, A., Suzuki, Y., Tame, J. R., Nagata, K. & Park, S. Y.
  • Journal Title: Embo J., accepted
  • Peer Reviewed
• [Journal Article] Mutational Analysis of the Substrate Specificity of Escherichia coli Penicillin Binding Protein 4 (PBP4) (2009)
  • Author(s): Clarke, T., Kawai, F., Park, S. Y., Tame, J., Dowson, C. & Roper, D.
  • Journal Title: Biochemistry 48 Pages: 2675-2683
  • Peer Reviewed
• [Journal Article] RNA and Protein Complexes of trp RNA-Binding Attenuation Protein Characterized by Mass Spectrometry (2009)
  • Author(s): Akashi, S., Watanabe, M., Heddle, J. G., Unzai, S., Park, S. Y. & Tame, J. R.
  • Journal Title: Anal Chem 81 Pages: 2218-2226
  • Peer Reviewed
• [Journal Article] The nature of the TRAP-Anti-TRAP complex. (2009)
  • Author(s): Watanabe M, Heddle JG, Kikuchi K, Unzai S, Akashi S, Park SY, Tame JR.
  • Journal Title: Proc Natl Acad Sci U S A. 106(7) Pages: 2176-81
  • Peer Reviewed
• [Journal Article] Intersubunit linker length as a modifier of protein stability: crystal structures and thermostability of mutant TRAP (2008)
  • Author(s): Watanabe, M., Mishima, Y., Yamashita, I., Park, S. Y., Tame, J. R. & Heddle, J. G.
  • Journal Title: Protein Sci 17 Pages: 518-26
  • Peer Reviewed
• [Journal Article] The structural basis for an essential subunit interaction in influenza virus RNA polymerase (2008)
  • Author(s): Obayashi, E., Yoshida, H., Kawai, F., Shibayama, N., Kawaguchi, A., Nagata, K., Tame, J. R. & Park, S. Y.
  • Journal Title: Nature 454 Pages: 1127-31
  • NAID: 10023987774
  • Peer Reviewed
• [Journal Article] The structural basis for an essential subunit interaction in influenza virus RNA polymerase. (2008)
  • Author(s): Obayashi E, Yoshida H, Kawai F, Shibayama N, Kawaguchi A, Nagata K, Tame JR, Park SY.
  • Journal Title: Nature 454(7208) Pages: 1127-31
  • NAID: 10023987774
  • Peer Reviewed
• [Journal Article] Intersubunit linker length as a modifier of protein stability : crystal structures and thermostability of mutant TRAP. (2008)
  • Author(s): Watanabe M, Mishima Y, Yamashita I, Park SY, Tame JR, Heddle JG.
  • Journal Title: Protein Sci. 17(3) Pages: 518-26
  • Peer Reviewed
• [Journal Article] Intersubunit linker length as a modifier of protein stability: crystal structures and thermostability of mutant TRAP. (2008)
  • Author(s): Watanabe M, Mishima Y, Yamashita I, Park SY, Tame JR, Heddle JG.
  • Journal Title: Protein Sci. 17(3) Pages: 518-26
  • Peer Reviewed
• [Journal Article] Effect of N-terminal residues on the structural stability of recombinant horse L-chain apoferritin in an acidic environment (2007)
  • Author(s): Yoshizawa, K., Mishima, Y., Park, S. Y., Heddle, J. G., Tame, J. R., Iwahori, K., Kobayashi, M. & Yamashita, I.
  • Journal Title: J Biochem 142 Pages: 707-13
  • Peer Reviewed
• [Journal Article] Limited tolerance towards folded elements during secretion of the autotransporter Hbp (2007)
  • Author(s): Jong, W. S., ten Hagen-Jongman, C. M., den Blaauwen, T., Slotboom, D. J., Tame, J. R., Wickstrom, D., de Gier, J. W., Otto, B. R. & Luirink, J.
  • Journal Title: Mol Microbiol 63 Pages: 1524-36
  • Peer Reviewed
• [Journal Article] Structure and mechanism of HpcG, a hydratase in the homoprotocatechuate degradation pathway of Escherichia coli (2007)
  • Author(s): Izumi, A., Rea, D., Adachi, T., Unzai, S., Park, S. Y., Roper, D. I. & Tame, J. R.
  • Journal Title: J Mol Biol 370 Pages: 899-91
  • Peer Reviewed
• [Journal Article] Crystallization and preliminary crystallographic studies of the metalloglycoprotein esterase A4 using a baculovirus expression system (2007)
  • Author(s): Hiraki, T., Shibayama, N., Yoon, Y. H., Yun, K. M., Hamamoto, T., Tame, J. R. & Park, S. Y.
  • Journal Title: Acta Crystallogr Sect F Struct Biol Cryst Commun 63 Pages: 734-6
  • Peer Reviewed
• [Journal Article] Dynamic allostery in the ring protein TRAP (2007)
  • Author(s): Heddle, J. G., Okajima, T., Scott, D. J., Akashi, S., Park, S. Y. & Tame, J. R.
  • Journal Title: J Mol Biol 371 Pages: 154-67
  • Peer Reviewed
• [Journal Article] Using the ring-shaped protein TRAP to capture and confine gold nanodots on a surface (2007)
  • Author(s): Heddle, J. G., Fujiwara, I., Yamadaki, H., Yoshii, S., Nishio, K., Addy, C., Yamashita, I. & Tame, J. R.
  • Journal Title: Small 3 Pages: 1950-6
  • Peer Reviewed
• [Journal Article] Nickel binding to NikA : an additional binding site reconciles spectroscopy, calorimetry and crystallography (2007)
  • Author(s): Addy, C., Ohara, M., Kawai, F., Kidera, A., Ikeguchi, M., Fuchigami, S., Osawa, M., Shimada, I., Park, S. Y., Tame, J. R. & Heddle, J. G.
  • Journal Title: Acta Crystallogr D Biol Crystallogr 63 Pages: 221-9
  • Peer Reviewed
• [Journal Article] Effect of N-terminal residues on the structural stability of recombinant horse L-chain apoferritin in an acidic environment. (2007)
  • Author(s): Yoshizawa K, Mishima Y, Park SY, Heddle JG, Tame JR, Iwahori K, Kobayashi M, Yamashita I.
  • Journal Title: J Biochem 142(6) Pages: 707-13
  • Peer Reviewed
• [Journal Article] Using the ring-shaped protein TRAP to capture and confine gold nanodots on a surface. (2007)
  • Author(s): Heddle JG, Fujiwara I, Yamadaki H, Yoshii S, Nishio K, Addy C, Yamashita I, Tame JR.
  • Journal Title: Small. 3(11) Pages: 1950-6
  • Peer Reviewed
• [Journal Article] Crystallization and preliminary crystallographic studies of the metallogl ycoprotein esterase A4 using a baculovirus expression system. (2007)
  • Author(s): Hiraki T, Shibayama N, Yoon YH, Yun KM, Hamamoto T, Tame JR, Park SY.
  • Journal Title: Acta Crystallogr Sect F Struct Biol Cryst Commun 63(Pt 9) Pages: 734-6
  • Peer Reviewed
• [Journal Article] Structure and mechanism of HpcG, a hydratase in the homoprotocatechuate degradation pathway of Escherichia coli. (2007)
  • Author(s): Izumi A, Rea D, Adachi T, Unzai S, Park SY, Roper DI, Tame JR.
  • Journal Title: J Mol Biol. 370(5) Pages: 899-911
  • Peer Reviewed
• [Journal Article] Dynamic allostery in the ring protein TRAP. (2007)
  • Author(s): Heddle JG, Okajima T, Scott DJ, Akashi S, Park SY, Tame JR.
  • Journal Title: J Mol Biol. 371(1) Pages: 154-67
  • Peer Reviewed
• [Presentation] Haemoglobin as a drug target (2008)
  • Author(s): Takeshi Yokoyama, Satoru Unzai, Sam-Yong Park, Jeremy
  • Organizer: The 40th crystallographic meeting at Erice
  • Place of Presentation: Italy
• [Presentation] Protonation states of buried histidine residues in human deoxyhemoglobin revealed by neutron crystallography (2008)
  • Author(s): Sam-Yong Park, Toshiyuki Chatake, Naoya Shibayama, Yukiko Morimoto
  • Organizer: XVth International Conference on Oxygen Binding and Sensing Proteins
  • Place of Presentation: Aarhus, Denmark
• [Presentation] インフルエンザウイルスのRNAポリメラーゼにおけるサブユニット間相互作用 (2008)
  • Author(s): 尾林栄治, 吉田尚史, 河合文啓,川口敦史, 永田恭介, Jeremy Tame,朴三用
  • Organizer: 第81回日本生化学会大会
  • Place of Presentation: 神戸
• [Presentation] What a crystal structure doesn't tell you (2008)
  • Author(s): Jeremy R. H. Tame
  • Organizer: よこはまNMR構造生物学研究会 国際シンポジウム
  • Place of Presentation: 横浜
• [Presentation] Structural insights into serine protease autotoransporter HBP from pathogenic Escherichia coli. (2008)
  • Author(s): J. Tame
  • Organizer: Society for General Microbiology
  • Place of Presentation: Edinburgh, UK
• [Presentation] X-ray Crystallography of Autotransporters from Pathogenic Bacteria (2007)
  • Author(s): Kaoru Nishimura, Sam-Yong Park, Jeremy
  • Organizer: Molecular Microbiology Young Microbiologists Minisymposium Prokaryotic Membranes
  • Place of Presentation: Paris, France
  • Year and Date: 2007-10-12
• [Presentation] X-ray Crystallography of Autotransporters from Pathogenic Bacteria. (2007)
  • Author(s): Kaoru Nishimura, Sam-Yong Park, and Jeremy Tame
  • Organizer: Molecular Microbiology Young Microbiologists Minisymposium Prokaryotic Membranes.
  • Place of Presentation: Paris, France
  • Year and Date: 2007-10-12
• [Presentation] Dynamic allostery in the tryptophan-binding protein TRAP (2007)
  • Author(s): Heddle JG, Akashi S, Park SY, Tame JR.
  • Organizer: Biophysics of ligand binding to drug tagets.
  • Place of Presentation: Strasbourg, France
• [Book] Dioxygen Binding and Sensing Proteins : A Tribute to Beatrice and Jonathan Wittenberg(編集 : Martino Bolognesi, Guido Di Prisco, Cinzia Verde, 出版社 : Springer)(ISBN 8847008069, 9788847008069) (2008)
  • Author(s): Satoru Unzai, Kiyohiro Imai, Sam-Yong Park, Kiyoshi Nagai, and Jeremy R.H.
  • Publisher: Mutagenic studies on the Origins of the Root Effect