Roles of tyrosine kinases during evolution of multicellular animals

• Project/Area Number: 19370053
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Functional biochemistry
• Research Institution: Osaka University
• Principal Investigator: OKADA Masato Osaka University, 微生物病研究所, 教授 (10177058)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000); Fiscal Year 2008: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000); Fiscal Year 2007: ¥10,660,000 (Direct Cost: ¥8,200,000、Indirect Cost: ¥2,460,000)
• Keywords: 多細胞動物 / 進化 / チロシンキナーゼ / リン酸化 / Src / Csk / 立襟鞭毛虫 / 線虫

Research Abstract

蛋白質のチロシン残基特異的リン酸化酵素(チロシンキナーゼ)は動物特有の酵素である。本研究では、多細胞動物の成立におけるチロシンキナーゼの意義を明らかするために、動物の祖先とされる単細胞原生生物(立襟鞭毛虫)、原始的な多細胞動物(海綿)、および、特殊に進化した線形動物(線虫)をモデルとして用いた解析を行い、チロシンキナーゼおよびその下流因子の活性制御系が動物の多細胞化の過程で発達あるいは特殊化してきたことを明らかにした。

Research Products

• [Journal Article] Non-receptor tyrosine kinase CSK-1 controls pharyngeal muscle organization in Caenorhabditis elegans (2009)
  • Author(s): Takata, N., Itoh, B., Misaki, K., Hirose, T., Yonemura, S., & Okada, M.
  • Journal Title: Genes Cells 14 Pages: 381-393
• [Journal Article] Ablation of Csk in neural crest lineages causes corneal anomaly by deregulating collagen fibril organization and cell motility (2008)
  • Author(s): Takatsuka, A., Yaga, R., Koike, M., Oneyama, C., Nada, S., Schmedt, C., Uchiyama, Y. & Okada, M.
  • Journal Title: Dev Biol 315 Pages: 474-488
• [Journal Article] Functional dissection of transformation by c-Src and v-Src. (2008)
  • Author(s): Oneyama, C., Hikita, T., Nada, S. & Okada, M.
  • Journal Title: Genes to Cells 13 Pages: 1-12
• [Journal Article] Functional dissection of transformation by c-Src and v-Src (2008)
  • Author(s): Chitose Oneyama
  • Journal Title: Genes to Cells 13 Pages: 1-12
  • Peer Reviewed
• [Journal Article] Ablation of Csk in neural crest lineages causes corneal anomaly by deregulating collagen fibril organization and cell motility (2008)
  • Author(s): Atsuko Takatsuka
  • Journal Title: Developmental Biology (in press)
  • Peer Reviewed
• [Journal Article] C-terminal Src kinase controls development and maintenance of mouse squamous epithelia. (2007)
  • Author(s): Yagi, R., Waguri, S., Sumikawa, Y., Nada, S., Oneyama, C., Itami, S., Schmedt, C., Uchiyama, Y. & Okada, M.
  • Journal Title: Embo J 26 Pages: 1234-1244
• [Journal Article] C-terminal Src kinase controls development and maintenance of mouse squamous epithelia (2007)
  • Author(s): Reiko Yagi
  • Journal Title: EMBO J 26 Pages: 1234-1244
  • Peer Reviewed
• [Journal Article] Non-receptor tyrosine kinase CSK-1 controls pharyngeal muscle organization in Caenorhabditis elegans
  • Author(s): Takata, N.
  • Journal Title: Genes to Cells (印刷中)
  • Peer Reviewed
• [Presentation] 哺乳動物においてSrcシグナルに関与するWntシグナル分子Prickleの機能解析 (2008)
  • Author(s): 高橋佑介
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2008-12-11
• [Presentation] Functional transition of proto-oncogenes during evolution ofmulticellularity in animals (2008)
  • Author(s): 渡利彰浩
  • Organizer: 第67回日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2008-10-28
• [Presentation] Functional transition of proto-oncogenes during evolution of multicellularity in animals. (2008)
  • Author(s): 渡利彰浩
  • Organizer: 第67回日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2008-10-28
• [Presentation] SRC-1 interacts with Wntsignaling protein PRKL-1/Prickle in C. elegans (2008)
  • Author(s): Itoh, B. et.al.
  • Organizer: 2008 East Asia Worm Meeting
  • Place of Presentation: Shanghai, China
  • Year and Date: 2008-04-20
• [Presentation] SRC-1 interacts with Wnt signaling protein PRKL-1/Prickle in C. elegans. (2008)
  • Author(s): Itoh, B.
  • Organizer: 2008 East Asia Worm Meeting
  • Place of Presentation: Shanghai, China
  • Year and Date: 2008-04-20
• [Presentation] CSK-1 controls pharyngeal muscle organization in C. elegans (2008)
  • Author(s): Takata, N. et.al.
  • Organizer: 2008 East Asia Worm Meeting
  • Place of Presentation: Shanghai, China
  • Year and Date: 2008-04-19
• [Presentation] CSK-1 controls pharyngeal muscle organization in C. elegans. (2008)
  • Author(s): Nozomu Takata
  • Organizer: 2008 East Asia Worm Meeting
  • Place of Presentation: Shanghai, China
  • Year and Date: 2008-04-19
• [Presentation] 線虫SRC-1はWntシグナルに関与する (2007)
  • Author(s): 伊東文祥
  • Organizer: 第30回日本分子生物学会年会・第80回日本生化学大会合同大会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2007-12-14
• [Presentation] 線虫の非受容体型チロシンキナーゼSrcの負の制御因子であるCSK-1は咽頭筋原繊維の構築を制御する (2007)
  • Author(s): 高田望
  • Organizer: 第30回日本分子生物学会年会・第80回日本生化学大会合同大会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2007-12-13
• [Presentation] 線虫C.elegansにおけるmechanosensoryneuronの極性決定に関わる遺伝子群のスクリーニング (2007)
  • Author(s): 増田瞳
  • Organizer: 第30回日本分子生物学会年会・第80回日本生化学大会合同大会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2007-12-12
• [Presentation] 線虫C. elegansにおけるmechanosensory neuronの極性決定に関わる遺伝子群のスクリーニング (2007)
  • Author(s): 増田瞳
  • Organizer: 第30回日本分子生物学会年会・第80回日本生化学大会合同大会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2007-12-12