Localization of transmembrane receptors and functional and structural compartentalization of the cytoplasmic membrane
Project/Area Number |
19370066
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Biophysics
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Research Institution | Hosei University |
Principal Investigator |
KAWAGISHI Ikuro Hosei University, 生命科学部, 教授 (80234037)
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Project Period (FY) |
2007 – 2009
|
Project Status |
Completed (Fiscal Year 2009)
|
Budget Amount *help |
¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000)
Fiscal Year 2009: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2008: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2007: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
|
Keywords | 大腸菌 / コレラ菌 / 走化性 / シグナル伝達 / ヒスチジンキナーゼ / 蛋白質複合体 / 緑色蛍光蛋白質 / 蛍光顕微鏡 / Hisキナーゼ / His キナーゼ |
Research Abstract |
The chemoreceptors and the histidine kinases of Escherichia coli as well as protein components of chemotaxis-related signaling systems were analyzed for their localization to obtain several important results concerning the mechanisms underlying localization and its control of transmembrane sensors. Movements of the chemoreceptor through the membrane were analyzed to find that they are restricted by actin-like cytoskeleton or structures associated with it. The chemoreceptor was labeled with FlAsH and HaloTag to establish new systems for localization analyses. These results shed new light on the localization/function of transmembrane sensors and the architecture of the cytoplasmic membrane in prokaryotic cells.
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Report
(4 results)
Research Products
(207 results)
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[Journal Article] Thermosensing function of the Escherichia coli redox sensor Aer.2010
Author(s)
Nishiyama, S., Ohno, S., Ohta, N., Inoue, Y., Fukuoka, H., Ishijima, A., Kawagishi, I.
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Journal Title
J. Bacteriol 192
Pages: 1740-1743
Related Report
Peer Reviewed
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[Journal Article] Thermosensing function of the Escherichia coli redox sensor Aer2010
Author(s)
Nishiyama, S., Ohno, S., Ohta, N., Inoue, Y., Fukuoka, H., Ishijima, A., Kawagishi, I.
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Journal Title
J.Bacteriol. 192
Pages: 1740-1743
Related Report
Peer Reviewed
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[Journal Article] Systematic Cys mutagenesis of FlgI, theflagellar P-ring component of Escherichia coli.2008
Author(s)
Hizukuri, Y., Kojima, S., Yakushi, T., Kawagishi, I., Homma, M.
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Journal Title
Microbiology 154
Pages: 810-817
Related Report
Peer Reviewed
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[Journal Article] Systematic Cys mutagenesis of Flgl,theflagellar P-ring component of Escherichia coli.2008
Author(s)
Hizukuri, Y., Kojima, S., Yakushi, T., Kawagishi, I., and Homma, M.
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Journal Title
Mierobiology 154
Pages: 810-817
Related Report
Peer Reviewed
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[Journal Article] Involvement of minor components associated with the FimA fimbriae of Porphyromonas gingivalis in adhesive functions.2007
Author(s)
Nishiyama, S., Murakami, Y., Nagata, H., Shizukuishi, S., Kawagishi, I., Yoshimura, F.
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Journal Title
Microbiology 153
Pages: 1916-1925
Related Report
Peer Reviewed
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[Journal Article] The voltage-gated Na+ channel NaVBP co-localizes with methyl-accepting chemotaxis protein at cell poles of alkaliphilic Bacillus pseudofirmus OF4.2007
Author(s)
Fujinami, S., Sato, T., Trimmer, J.S., Spiller, B.W., Clapham, D.E., Krulwich, T.A., Kawagishi, I., Ito, M.
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Journal Title
Microbiology 153
Pages: 4027-4038
Related Report
Peer Reviewed
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[Journal Article] Involvement of minor components associated with the FimA fimbriae of Porphyromonas gingivalis in adhesive functions.2007
Author(s)
Nishiyama, S. , Murakami, Y., Nagata, H., Shizukuishi, S., Kawagishi, I., and Yoshimura, F.
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Journal Title
Mierobiology 153
Pages: 1916-1925
Related Report
Peer Reviewed
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[Journal Article] The voltage-gated Na^+ channel Na_vBP co-localizes with methyl-accepting chemotaxis Protein at Cell Poles of Alkaliphilic Bacillus pseudofirmus OF4.2007
Author(s)
Fujinami, S., Sato, T., Trimmer, J.S., Spiller, B.W., Clapham, D.E.. , Krulwich, T.A., Kawaishi, I.and Ito, M.
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Journal Title
Mierobiology 153
Pages: 4027-4038
Related Report
Peer Reviewed
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[Journal Article]
Author(s)
入枝泰樹, 太田徳子, 川岸郁朗
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Journal Title
自己組織化ハンドブック(下村政嗣, 山口智彦 編)(株式会社エヌ・ティー・エス) 2009
Pages: 365-374
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[Presentation] Thermosensing function of Aer, a redox sensor of E. coli2009
Author(s)
Nishiyama, S., Ohno, S. N. Ohta, N., Inoue, Y., Fukuoka, H., Ishijima, A., Kawagishi, I.
Organizer
Bacterial Locomotion and Signal Tranduction X.
Place of Presentation
Cuerunavaca, Mexico.
Related Report
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[Presentation] Localization patterns of the histidine kinases in an Escherichia coli cell2009
Author(s)
Inaba, T., Banno, S., Sawaki, H., Yamakawa, A., Yoshimoto, M., Homma, M., Kawagishi. I
Organizer
Bacterial Locomotion and Signal Tranduction X
Place of Presentation
Cuerunavaca, Mexico.
Related Report
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[Presentation] Role of the HAMP domain of the bacterial chemoreceptor in signaling and subcellular localization.2008
Author(s)
Ohta, N., Irieda, H., Banno, S., Homma, M., Kawagishi, I.
Organizer
JSPS-DST Asian Academic Seminar 2008.
Place of Presentation
Bangalore, India
Related Report
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[Presentation] Localization control of components of the chemotaxis-related signaling systems in Vibrio cholerae.2008
Author(s)
Hiremath, G., Ebisawa, T., Hyakutake, A., Banno, S., Nishiyama, S., Kawagishi, I.
Organizer
JSPS-DST Asian Academic Seminar 2008.
Place of Presentation
Bangalore, India
Related Report
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[Presentation] 大腸菌アミノ酸受容体のリガンド識別機構.2008
Author(s)
田島寛隆, 今田勝巳, 佐久間麻由子, 本間道夫, 川岸郁朗
Organizer
文部科学省科学研究費補助金特定領域「マルチスケール操作によるシステム細胞工学」第5回公開シンポジウム
Place of Presentation
東京都千代田区(法政大学市ヶ谷キャンパス)
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[Presentation] Role of the HAMP domain of the bacterial chemoreceptor in signaling and subcellular localization2008
Author(s)
Ohta, N., Irieda, H., Banno, S., Homma, M., Kawagishi, I.
Organizer
JSPS-DST Asian Academic Seminar 2008.
Place of Presentation
Bangalore, India
Related Report
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[Presentation] Localization control of components of the chemotaxis-related signaling system Ill in Vibrio cholerae2008
Author(s)
Hiremath, G., Ebisawa, T., Hyakutake, A., Suzuki, D., Nishiyama, S., Homma, M., Kawagishi. I.
Organizer
日本生物物理学会第46回年会
Place of Presentation
福岡市(福岡国際会議場)
Related Report
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[Presentation] Aerobic/anaerobic control of localization of signaling components of Vibrio cholerae2008
Author(s)
Ebisawa, T., Hyakutake, A., Suzuki, D., Nishiyama, S., Hornma, M., Kawagishi. I.
Organizer
日本生物物理学会第46回年会
Place of Presentation
福岡市(福岡国際会議場)
Related Report
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[Presentation] Localization control of components of the chemotaxis-related signaling systems in Vibrio cholerae2008
Author(s)
Hiremath, G., Ebisawa, T., Hyakutake, A., Banno, S., Nishiyama, S., Kawagishi, I.
Organizer
JSPS-DST Asian Academic Seminar 2008.
Place of Presentation
Bangalore, India
Related Report
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