Crystallographic analysis of higher plant phytochrome

• Project/Area Number: 19370070
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biophysics
• Research Institution: Osaka Prefecture University
• Principal Investigator: TOKUTOMI Satoru Osaka Prefecture University, 大学院・理学系研究科, 教授 (90142009)
• Co-Investigator(Kenkyū-buntansha): NAKASAKO Masayoshi 慶応義塾大学, 理工学部, 教授 (30227764) ; YOSHIHARA Shizue 大阪府立大学, 大学院・理学系研究科, 助教 (20382236)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥11,050,000 (Direct Cost: ¥8,500,000、Indirect Cost: ¥2,550,000); Fiscal Year 2008: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2007: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
• Keywords: 植物 / 光受容体 / フィトクロム / フォトトロピン / 結晶構造解析 / 植物光受容体 / 分子構造 / 光変換反応

Research Abstract

固着生活を送る植物は光を重要な環境情報として用いており、進化の過程で主に赤色光領域の光受容体であるフィトクロムとフォトトロピンなどの複数の青色光受容体を獲得してきた。本研究ではフィトクロムの結晶構造解析を目指して研究を行ったが結晶を得ることができなかった。結晶化条件スクリーニングで幾つかの有望な結果を得て引き続き研究を行っている。一方、当初目的にはなかったが、フォトトロピンに関して、シロイヌナズナの2種類のフォトトロピンの光受容ドメインの一つであるLOV1の結晶構造解析を行い、LOVドメインの初めての二量体構造を明らかにした。

Research Products

• [Journal Article] Different role of the Jalpha helix in the light-induced activation of the LOV2 domains in various phototropins. (2009)
  • Author(s): Koyama, T., Iwata, T., Yamamoto, A., Sato, Y., Matsuoka, D., Tokutomi, S., Kandori, H.
  • Journal Title: Biochemistry 48巻 Pages: 7621-7618
  • Peer Reviewed
• [Journal Article] Oligomeric State-Dependent Conformational Change of the BLUF Protein TePixD (T110078) (2009)
  • Author(s): Tanaka, K., Nakasone, Y., Okajima, K., Ikeuchi, M., Tokutomi, S., Terazima, M.
  • Journal Title: J.Mot.Bio 1.386巻 Pages: 1290-1230
  • Peer Reviewed
• [Journal Article] Light Signal Transduction Pat hway from the Flavin Chromophore to the Ja helix of (2009)
  • Author(s): Yamamoto, A., Iwata, T., Sato, Y., Matsuoka, D., Tokutomi, S., Kandori. H.
  • Journal Title: Arabidpsis Phototropinl.Biophys.J. 96巻 Pages: 2771-2778
  • Peer Reviewed
• [Journal Article] Vibra tional Assignment of the Flavin-Cyst einyl Adduct in a Signaling State of the LOV Domain in FKF1. (2009)
  • Author(s): Kikuchi, S., Unno, M., Zikihara, K., Tokutomi, S., Yamauchi, S.
  • Journal Title: J.Phys.Chem. 113巻 Pages: 2913-2921
  • Peer Reviewed
• [Journal Article] Time-Resolved FT-IR Study on Photoadduct Formation and Secondary Structural Changes within the Phototropin LOV Domain (2009)
  • Author(s): Pfeifer A., Majerus, T., Zikihara, K., Matsuoka, D., Tokutomi, S., Heberle, J., Kottke, T.
  • Journal Title: Biophys J 96巻 Pages: 1462-1470
  • Peer Reviewed
• [Journal Article] Oligomeric structure of LOV domains in Arabidopsis phototropin. (2009)
  • Author(s): Katsura, H., Zikihara, K., Okajima, K., Yoshihara, S., Tokutomi, S.
  • Journal Title: FEBS Lett. 583巻 Pages: 526-530
  • Peer Reviewed
• [Journal Article] Stability of Dimer and Domain-Domain Interaction of Arabidop sis Phototropin 1 LOV2. (2008)
  • Author(s): Nakasone, Y., Eitoku, T., Zikihara, K., Matsuoka, D., Tokutomi, S., T erazima, M.
  • Journal Title: J.Mot.Biol. 373巻 Pages: 904-913
  • Peer Reviewed
• [Journal Article] Structural Basis of the LOV1 Dimerization of Arabidopsis Phototropins 1 and 2. (2008)
  • Author(s): Nakasako, M., Zikihara, K., Matsuoka, D., Katsura, H., Tokutomi, S.
  • Journal Title: J.Mot.Biol 381巻 Pages: 718-733
  • NAID: 130006993861
  • Peer Reviewed
• [Journal Article] rystallization and preliminary X-ray diffraction analysis of the LOV1 dom ains of phototropin 1 and 2 from Ara bidopsis thaliana (2008)
  • Author(s): Nakasako, M., Hirata, M., Shimizu, N., Hosokawa, S., Matsuoka, D., Oka, T., Yamamoto, M., Tokutomi, S. C
  • Journal Title: Acta Crystallogr. Sect F 64巻 Pages: 617-621
  • Peer Reviewed
• [Journal Article] Involvement of e lectron transfer in the photoreaction of Zebrafish Cryptochrome-DASH. (2008)
  • Author(s): Zikihara, K., Ishikawa, T., Todo, T., Tokutomi, S.
  • Journal Title: Photochem.Photobiol. 84巻 Pages: 1016-1023
  • Peer Reviewed
• [Journal Article] Role of Phe1010 in the Light-Induced Structural Changes of the neo1-LOV2 Domain of Adiantum. (2008)
  • Author(s): Yamamoto, A., Iwata, T., Tokutomi, S., Kandori, H.
  • Journal Title: Biochemistry 47巻 Pages: 922-928
  • Peer Reviewed
• [Journal Article] Structural Basis of the LOV1 Dimerization of Arabidopsis Phototropins 1 and 2. (2008)
  • Author(s): Nakasako, M., Zikihara, K., Mtsuoka, D., Katsura, H., Tokutomi, S.
  • Journal Title: J. Mol. Biol. 381 Pages: 718-733
  • NAID: 130006993861
  • Peer Reviewed
• [Journal Article] Role of Phe1010 in the Light-Induced Structural Changes of the neol-LOV2 Domain of Adiantum. (2008)
  • Author(s): Yamamoto, A., Iwata, T., Tokutomi, S. and Kandori, H.
  • Journal Title: Biochemistry 47 Pages: 922-928
  • Peer Reviewed
• [Journal Article] Molecular structure and regulation of phototropin kinase by blue tight (2007)
  • Author(s): Tokutomi, S., Matsuoka, D., Zikihara, K.
  • Journal Title: Biochim.Biophys. Acta1784巻 Pages: 133-142
  • Peer Reviewed
• [Journal Article] Primary processes during the light-signal transduction of phototropin (2007)
  • Author(s): Matsuoka, D., Iwata, T., Zikihara, K., Kandori, H., Tokutomi, S.
  • Journal Title: Photochem.Photobiol 83巻 Pages: 122-130
  • Peer Reviewed
• [Journal Article] Dynamics of conformational changes of Arabi dopsis phototropin 1 LOV2 with the 1 inker domain (2007)
  • Author(s): Nakasone, Y., Eitoku, T., Matsuoka, D., Tokutomi, S., Terazima, M.
  • Journal Title: J.Mol.Biol. 367巻 Pages: 432-442
  • Peer Reviewed
• [Journal Article] Photochemical intermediates of Arabidopsis phototropin 2 LOV2 asso ciated with conformational changes (2007)
  • Author(s): Eitoku, T., Nakasone, Y., Zikihara, K., Matsuoka, D., Tokutomi, S., Terazima, M.
  • Journal Title: J.Mol.Biol 371巻 Pages: 1290-1303
  • Peer Reviewed
• [Journal Article] He terogeneous Environment of the S-H Group of Cys966 near the Flavin Chrom ophore in the LOV2 Domain of Adiantum (2007)
  • Author(s): Sato, Y., Nabeno, M., Iwata, T., Tokutomi, S., Sakurai, M., Kandori, N.
  • Journal Title: Neochromel.Biochemistry 46巻 Pages: 10258-10265
  • Peer Reviewed
• [Journal Article] Hydration and Tempera ture Similarly Affect Light-Induced Protein Structural Changes in the Chromophoric Domain of Phototropin (2007)
  • Author(s): Iwata, T., Yamamoto, A., Tokutomi, S., Kandori, H.
  • Journal Title: Biochemistry 46巻 Pages: 7016-7021
  • Peer Reviewed
• [Journal Article] Dynamics of conformational changes of Arabidopsis phototropin 1 LOV2 with the linker domain. (2007)
  • Author(s): Nakasone, Y., Eitoku T., Matsuka, D., Tokutomi, S. and Terazima, M.
  • Journal Title: J.Mol.Biol. 367 Pages: 432-42
  • Peer Reviewed
• [Journal Article] Molecular structure and regulation of phototropin kinase by blue light. (2007)
  • Author(s): Tokutomi, s., Matsuoka, D. and Zikihara, K.
  • Journal Title: Biochim.Biophys.Acta 1784 Pages: 133-42
  • Peer Reviewed
• [Presentation] Molecular basis for regulation of phototropin kinase by blue light (2009)
  • Author(s): Tokutomi, S.
  • Organizer: Memorial Symposium for the 24^<th> Intern ational Prize for Biology, Celebrating Dr. Winslow R. Briggs "Biology of Sensing"
  • Place of Presentation: Kyoto
• [Presentation] Photoreaction of a Blue/Green Reversible Phytochrome-Like Photoreceptor Studied By Low-Temperature Spectroscopy (2009)
  • Author(s): Yoshihara, S., Zikihara, K. Tokutomi, S.
  • Organizer: International Conference on Tetrapyrrole Photoreceptors of Photosynthetic Organisms
  • Place of Presentation: Montlay CA USA
• [Presentation] Regulation of phototropin kinase by blue light (2009)
  • Author(s): Tokutomi, S.
  • Organizer: 15^th International Congress on Photobiology
  • Place of Presentation: Dusseldorf Germany
• [Presentation] フォトトロピンの光受容ドメインの分子構造、光反応、キナーゼ活性光制御 (2009)
  • Author(s): 徳富哲, 直原一徳, 岡島公司, 桂ひとみ, 吉原静恵
  • Organizer: 日本植物生理学会第50回年会, シンポジウム
  • Place of Presentation: 名古屋
• [Presentation] How blue light regulates kinase activity of phototropin (2008)
  • Author(s): Tokutomi, S.
  • Organizer: 34^th Annual Meeting of American Society for Photobiology
  • Place of Presentation: San Francisco USA
• [Presentation] Structural basis for the photoreception of Arabidpsis phototropin (2008)
  • Author(s): Tokutomi, S., Zikihara, K., Okajima, K., Katsura, H., Yoshihara, S., Nakasako, M.
  • Organizer: 15^th Anniversary Meeting for Korean Society of Photoscience
  • Place of Presentation: Jeju Korea
• [Presentation] シロイヌナズナ・フォトトロピン1および2、LOVドメインのダイマー形成分子基盤 (2008)
  • Author(s): 中迫雅由, 直原一徳, 松岡大介, 桂ひとみ, 徳富哲
  • Organizer: 日本植物生理学会第49回年会
  • Place of Presentation: 札幌
• [Presentation] Structural basis of the light regulation of phototropin Kivase (2008)
  • Author(s): Tokutomi, S.
  • Organizer: Gordon Research Conference
  • Place of Presentation: Ventura CA USA
• [Presentation] Structural basis for the photoreception of Arabidpsis phototropin (2008)
  • Author(s): Tokutomi. S., Zikihara, K., Okajima, K, Katsura, H., Yoshihara, S., Nakasako, M
  • Organizer: 15^<th> Anniversary Meeting for Korean Society of Photscience
  • Place of Presentation: Korea, Jeju
• [Presentation] シロイヌナズナ・フォトトロピン1および2、LOV1ドメインのダイマー形成分子基盤 (2008)
  • Author(s): 中迫 雅由、直原 一徳、松岡 大介、桂 ひとみ、徳富 哲
  • Organizer: 日本植物生理学会第49回年会
  • Place of Presentation: 札幌
• [Presentation] Structural basis of the light regulation of phototropin kinase (2008)
  • Author(s): Tokutomi, S.
  • Organizer: Gordon Research Conference:Photosensory Receptors & Signal Transduction
  • Place of Presentation: Ventuta,CA,USA
• [Presentation] シロイヌナズナ・フォトトロピン1および2LOV1ドメインの結晶構造解析 (2007)
  • Author(s): 中迫雅由, 松岡大介, 直原一徳, 桂ひとみ, 徳富哲
  • Organizer: 日本生物物理学会第45回年会
  • Place of Presentation: 横浜
• [Presentation] シロイヌナズナ・フォトトロピン1および2LOV1ドメインの結晶構造解析 (2007)
  • Author(s): 中迫 雅由、松岡 大介、直原 一徳、桂 ひとみ、徳富 哲
  • Organizer: 日本生物物理学会第45同年会
  • Place of Presentation: 横浜
• [Remarks]
  • URL: http://www.b.s.osakafu-u.ac.jp/~toxan/home.html