| Budget Amount *help |
¥13,520,000 (Direct Cost: ¥10,400,000、Indirect Cost: ¥3,120,000)
Fiscal Year 2009: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2008: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2007: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
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| Research Abstract |
Activity of a G protein-coupled receptor (GPCR) is evoked upon agonist binding via yet unknown dynamics of the protein moiety. We have crystallized prototypical GPCR rhodopsin, in the presence of some retinal derivatives that would bind to the surface of the protein and affect the activity. The binding of β-ionone, which is a truncated form of retinal and is supposed to have some allosteric effect, has been detected by X-ray diffraction analysis to 2.6 angstroms resolution. This is the first example demonstrating that the hydrophobic surface of a GPCR could be occupied by a specifically bound small molecule.
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