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Regulation of homologous recombination through sumoylation of Rad52

Research Project

Project/Area Number 19370073
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Molecular biology
Research InstitutionTohoku University

Principal Investigator

SEKI Masayuki  Tohoku University, 大学院・薬学研究科, 准教授 (70202140)

Project Period (FY) 2007 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000)
Fiscal Year 2008: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000)
Fiscal Year 2007: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000)
KeywordsUbc9 / SUMO / Rad52 / Rad51 / 複製チェックポイント / 相同組換え / SUMO化 / 出芽酵母 / ChIP on chip / チェックポイント / 核移行
Research Abstract

相同組換えの中心酵素Rad52は、複製チャックポイントの欠損株で誘導される通常は活性化しない複製開始点領域に蓄積後、SUMO修飾を受けることを明らかにした。Rad52のSUMO化には、Rad52のオリゴマー形成、核への移行などが事前に必要であり、Rad52が機能する場にリクルートされてSUMO化を受け、Rad51の一本鎖DNAへのリクルートを部分的に促進し、それとカップルしてRad52の脱SUMO化が起こる制御機構を世界ではじめて提唱した。

Report

(3 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • Research Products

    (44 results)

All 2009 2008 2007

All Journal Article (38 results) (of which Peer Reviewed: 37 results) Presentation (4 results) Book (2 results)

  • [Journal Article] Accumulation of sumoylated Rad52 in checkpoint mutants perturbed in DNA replication2009

    • Author(s)
      Ohuchi, T., Seki, M., Kugou, K., Tada, S., Ohta, K., Enomoto, T.
    • Journal Title

      DNA Repair 8

      Pages: 690-696

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Generation and characterization of cells that can be conditionally depleted of mitochondrial SOD22009

    • Author(s)
      Takada, S., Inoue, E., Tano, K., Yoshii, H., Abe, T., Yoshimura, A., Akita, M., Tada, S., Watanabe, M., Seki, M., Enomoto, T.
    • Journal Title

      Biochem.Biophys.Res.Commun. 379(2)

      Pages: 233-238

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Repression of nascent strand elongation by deregulated Cdt1 during DNA replication in Xenopus egg extracts2009

    • Author(s)
      Tsuyama, T., Watanabe, S., Aoki, A., Cho, Y., Seki, M, Enomoto, T, Tada, S.
    • Journal Title

      Mol.Biol.Cell. 20

      Pages: 937-947

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Accumulation of sumoylated Rad52 in checkpoint mutants perturbed in DNA replication2009

    • Author(s)
      Ohuchi T, Seki M, Kugou K, Tada S, Ohta K, Enomoto T.
    • Journal Title

      DNA Repair (印刷中)

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Repression of nascent strand elongation by deregulated Cdtl during DNA replication in Xenopusegg extracts2009

    • Author(s)
      Tsuyama T, Watanabe S, Aoki A, Cho Y, Seki M, Enomoto T, Tada S.
    • Journal Title

      Mol. Biol. Cell 20

      Pages: 937-947

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A novel role for Rad17 in homologous recombination2008

    • Author(s)
      Nishino, K., Inoue, E., Takada, S., Abe, T., Akita, M., Yoshimura, A., Tada, S., Kobayashi, M., Yamamoto, K., Seki, M., Enomoto, T.
    • Journal Title

      Genes Genet.Syst. 83

      Pages: 427-431

    • NAID

      10023888077

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Nuclear localization of Rad52 is pre-requisite for its sumoylation2008

    • Author(s)
      Ohuchi, T., Seki, M., Enomoto, T.
    • Journal Title

      Biochem.Biophys.Res.Commun. 372

      Pages: 126-130

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Rad52 sumoylation and its involvement in the efficient induction of homologous recombination2008

    • Author(s)
      Ohuchi, T., Seki, M., Branzei, D., Maeda, D., Ui, A., Ogiwara, H., Tada, S., Enomoto, T.
    • Journal Title

      DNA Repair 7

      Pages: 879-889

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Analyses of functional interaction between RECQL1, RECQL5, and BLM which physically interact with DNA topoisomerase IIIα2008

    • Author(s)
      Otsuki, M., Seki, M., Inoue, E., Abe, T., Narita, Y.,Yoshimura, A., Tada, S., Ishii, Y., Enomoto, T.
    • Journal Title

      Biochim.Biophys.Acta 1782

      Pages: 75-81

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Vertebrate WRNIP1 and BLM are required for efficient maintenance of genome stability2008

    • Author(s)
      Hayashi, T., Seki, M., Inoue, E.,, Yoshimura, A., Kusa, Y., Tada, S., Enomoto, T.
    • Journal Title

      Genes Genet.Syst. 83

      Pages: 95-100

    • NAID

      10024395708

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] RecQ family helicases in genome stability : lessons from gene disruption studies in DT40 cells2008

    • Author(s)
      Seki, M., Otsuki, M., Ishii, Y., Tada, S., Enomoto, T.
    • Journal Title

      Cell Cycle 7

      Pages: 2472-2478

    • Related Report
      2008 Final Research Report
  • [Journal Article] Nuclear localization of Rad52 is pre-requisite for its sumoylation2008

    • Author(s)
      Ohuchi T, Seki M, Enomoto T.
    • Journal Title

      Biochem. Biophys. Res. Commun. 372

      Pages: 126-130

    • Related Report
      2008 Annual Research Report 2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Analyses of functional interaction betweon RECQL1, RECQL5, and BLM which physically interact with DNA topoisomerase IIIα2008

    • Author(s)
      Otsuki M, Seki M, Inoue E, Abe T, Narita Y, Yoshimura A, Tada S, Ishii Y, Enomoto T
    • Journal Title

      Biochim. Biophys. Acta. 1782

      Pages: 75-81

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Rad52 sumoylation and its involvement in the efficient induction of homologous recombination2008

    • Author(s)
      Ohuchi T, Seki M, Branzei D, Maeda D, Ui A, Ogiwara H, Tada S, Enomoto T.
    • Journal Title

      DNA Repair 7

      Pages: 879-889

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Analyses of functional interaction between RECQL1, RECQL5, and BLM which physically interact with DNA topoisomerase IIIa2008

    • Author(s)
      Otsuki M, Seki M, Inoue E, Abe T, Narita Y, Yoshimura A, Tada S, Ishii Y, Enomoto T
    • Journal Title

      Biochim. Biophys. Acta. 1782

      Pages: 75-81

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Functional interactions between BLM and XRCC3 in the cell2007

    • Author(s)
      Otsuki, M., Seki, M., Inoue, E., Yoshimura, A., Kato, G., Yamanouchi, S., Kawabe, Y., Tada, S., Shinohara, A., Komura, J., Ono, T., Takeda, S., Ishii, Y., Enomoto, T.
    • Journal Title

      J.Cell Biol. 179

      Pages: 53-63

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Ctf18 is required for homologous recombination-mediated double-strand break repair2007

    • Author(s)
      Ogiwara. H., Ohuchi, T,, Ui, A., Tada, S., Enomoto, T., Seki, M.
    • Journal Title

      Nucleic Acids Res. 35

      Pages: 4989-5000

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Rmi1, a member of the Sgs1-Top3 complex in budding yeast, contributesto sister chromatidcohesion2007

    • Author(s)
      Lai, M.S., Seki, M., Ui, A., Enomoto, T.
    • Journal Title

      EMBO Rep. 8

      Pages: 685-690

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Possible involvement of RecQL4 in the repair of double-strand DNA breaks in Xenopus egg extracts2007

    • Author(s)
      Kumata Y, Tada S, Yamanada Y, Tsuyama T, Kobayashi T, Dong YP, Ikegami K, Murofushi H, Seki M, Enomoto T.
    • Journal Title

      Biochim.Biophys.Acta. 1773

      Pages: 556-564

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Activation of a novel pathway involving Mms1 and Rad59 in sgs1 cells2007

    • Author(s)
      Ui A, Seki M, Ogiwara H, Lai MS, Yamamoto K, Tada S, Enomoto T
    • Journal Title

      Biochem.Biophys.Res.Commun. 356

      Pages: 1031-1037

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] WRN functions in a RAD18-dependent damage avoidance pathway2007

    • Author(s)
      Dong, Y.P., Seki, M., Yoshimura, A., Inoue, E., Furukawa, S., Tada, S., Enomoto, T.
    • Journal Title

      Biol.Pharm.Bull. 30

      Pages: 1080-1083

    • NAID

      110006278395

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Rad50 is involved in MMS-induced recombination between homologous chromosomes in mitotic cells2007

    • Author(s)
      Tomizawa Y, Ui A, Onoda F, Ogiwara H, Tada S, Enomoto T, Seki, M
    • Journal Title

      Genes Genet.Syst. 82

      Pages: 157-160

    • NAID

      10024394124

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] The INO80 chromatin remodeling complex functions in sister chromatid cohesion2007

    • Author(s)
      Ogiwara, H., Enomoto, T., Seki, M.
    • Journal Title

      Cell Cycle 6

      Pages: 1090-1095

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Actin-related protein Arp4 functions in kinetochore assembly2007

    • Author(s)
      Ogiwara, H., Ui, A., Kawashima, S., Kugou, K., Onoda, F., Iwahashi, H., Harata, M., Ohta, K., Enomoto, T., Seki, M.
    • Journal Title

      Nucleic Acids Res. 35

      Pages: 3109-3117

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] The INO80 complex is required for damage-induced recombination2007

    • Author(s)
      Kawashima, S., Ogiwara, H., Tada, S., Harata, M., Wintersberger, U., Enomoto, T., Seki, M.
    • Journal Title

      Biochem.Biophys.Res.Commun. 355

      Pages: 835-841

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] WRN counteracts the NHEJ pathway upon camptothecin exposure2007

    • Author(s)
      Otsuki, M., Seki, M., Kawabe, Y., Inoue, E., Dong, Y.P., Abe, T., Kato, G., Yoshimura, A., Tada, S., Enomoto, T.
    • Journal Title

      Biochem.Biophys.Res.Commun. 355

      Pages: 477-482

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Chl1 and Ctf4 are required for damage-induced recombinations2007

    • Author(s)
      Ogiwara, H., Ui, A., Lai, M.S., Enomoto, T, Seki, M.
    • Journal Title

      Biochem.Biophys.Res.Commun. 354

      Pages: 222-226

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Role of Elg1 protein in double strand break repair2007

    • Author(s)
      Ogiwara, H., Ui, A., Enomoto, T., Seki, M.
    • Journal Title

      Nucleic Acids Res 35

      Pages: 353-362

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Functional interactions between BLM and XRCC3 in the cell2007

    • Author(s)
      Otsuki M, Seki M, Inoue E, Yoshimura A, Kato G, Yamanouchi S, Kawabe Y, Tada S, Shinohara A, Komura J, Ono T, Takeda S, Ishii Y, Enomoto T.
    • Journal Title

      J. Cell Biol. 179

      Pages: 53-63

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Ctf18 is required for homologous recombination-mediated double-strand break repair2007

    • Author(s)
      Ogiwara. H., Ohuchi, T, Ui, A., Tada, S., Enomoto, T., Seki, M.
    • Journal Title

      Nucleic Acids Res. 35

      Pages: 4989-5000

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Rmi1, a member of the Sgs1-Top3 complex in budding yeast, contributesto sister chromatid cohesion2007

    • Author(s)
      Lai, M. S., Seki. M., Ui, A., Enomoto, T.
    • Journal Title

      EMBO Rep. 8

      Pages: 685-690

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Activation of a novel pathway involving Mms1 and Rad59 in sgs1 cells2007

    • Author(s)
      Ui A, Seki M, Ogiwara H, Lai MS, Yamamoto K, Tada S, Enomoto T.
    • Journal Title

      Biochem. Biophys. Res. Commun. 356

      Pages: 1031-1037

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Possible involvement of RecQL4 in the repair of double-strand DNA breaks in Xenopus egg extracts2007

    • Author(s)
      Kumata Y, Tada S, Yamanada Y, Tsuyama T, Kobayashi T, Dong YP, Ikegami K, Murofushi H, Seki M, Enomoto T.
    • Journal Title

      Biochim. Biophys. Acta. 1773

      Pages: 556-564

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] WRN counteracts the NHEJ pathway upon camptothecin exposure2007

    • Author(s)
      Otsuki M, Seki M, Kawabe Y, Inoue E, Dong YP, Abe T, Kato G, Yoshimura A, Tada S, Enomoto T.
    • Journal Title

      Biochem. Biophys. Res. Commun. 355

      Pages: 477-482

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] WRN functions in a RAD18-dependent damage avoidance pathway2007

    • Author(s)
      Dong YP, Seki M, Yoshimura A, Inoue E, Furukawa S, Tada S, Enomoto T
    • Journal Title

      Biol. Pharm. Bull. 30

      Pages: 1080-1083

    • NAID

      110006278395

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Rad50 is involved in MMS-induced recombination between homologous chromosomes in mitotic cells2007

    • Author(s)
      Tomizawa Y, Ui A, Onoda F, Ogiwara H, Tada S, Enomoto T, Seki M.
    • Journal Title

      Genes Genet. Syst. 82

      Pages: 157-160

    • NAID

      10024394124

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The INO80 chromatin remodeling complex functions in sister chromatid cohesion2007

    • Author(s)
      Ogiwara H, Enomoto T, Seki M.
    • Journal Title

      Cell Cycle 6

      Pages: 1090-1095

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Actin-related protein Arp4 functions in kinetochore assembly2007

    • Author(s)
      Ogiwara H, Ui A, Kawashima S, Kugou K, Onoda F, Iwahashi H, Harata M, Ohta K, Enomoto T, Seki M.
    • Journal Title

      Nucleic Acids Res. 35

      Pages: 3109-3117

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] Takemi Enomoto Requisite conditions for sumoylation of Rad52 and enhancement of the modification in checkpoint mutants2008

    • Author(s)
      Takashi Ohuchi, Masayuki Seki
    • Organizer
      第31回日本分子生物学会年会(第81回日本生化学会大会)合同大会
    • Place of Presentation
      神戸ポートアイランド
    • Year and Date
      2008-12-12
    • Related Report
      2008 Final Research Report
  • [Presentation] Requisite conditions for sumoylation of Rad52 and enhancement of the modification in checkpoint mutants2008

    • Author(s)
      Takashi Ohuchi, Masayuki Seki, Takemi Enomoto
    • Organizer
      第31回日本分子生物学会年会第81回日本生化学会大会合同大会
    • Place of Presentation
      神戸ボートアイランド
    • Year and Date
      2008-12-12
    • Related Report
      2008 Annual Research Report
  • [Presentation] Requirement of specific amino acids in Rad52 for sumoylation and recombination2007

    • Author(s)
      関政幸
    • Organizer
      第三回Japan-US DNA Repair Meeting
    • Place of Presentation
      仙台 秋保 ホテル クレセント
    • Year and Date
      2007-05-09
    • Related Report
      2008 Final Research Report
  • [Presentation] Requirement of specific amino acids in Rad52 for sumoylation and recombination2007

    • Author(s)
      関政幸
    • Organizer
      第三回 Japan-US DNA Repair Meeting
    • Place of Presentation
      仙台市/秋保/ホテルク レセント
    • Year and Date
      2007-05-09
    • Related Report
      2007 Annual Research Report
  • [Book] 蛋白質 核酸 酵素「別冊 ; 染色体サイクル」(Vol.54)2009

    • Author(s)
      関政幸/Mong Sing Lai/榎本武美
    • Publisher
      共立出版
    • Related Report
      2008 Final Research Report
  • [Book] 染色体サイクル2009

    • Author(s)
      関政幸/Mong Sing Lai/榎本武美
    • Total Pages
      4
    • Publisher
      共立出版
    • Related Report
      2008 Annual Research Report

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Published: 2007-04-01   Modified: 2016-04-21  

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