Regulation of homologous recombination through sumoylation of Rad52

• Project/Area Number: 19370073
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Molecular biology
• Research Institution: Tohoku University
• Principal Investigator: SEKI Masayuki Tohoku University, 大学院・薬学研究科, 准教授 (70202140)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000); Fiscal Year 2008: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000); Fiscal Year 2007: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000)
• Keywords: Ubc9 / SUMO / Rad52 / Rad51 / 複製チェックポイント / 相同組換え / SUMO化 / 出芽酵母 / ChIP on chip / チェックポイント / 核移行

Research Abstract

相同組換えの中心酵素Rad52は、複製チャックポイントの欠損株で誘導される通常は活性化しない複製開始点領域に蓄積後、SUMO修飾を受けることを明らかにした。Rad52のSUMO化には、Rad52のオリゴマー形成、核への移行などが事前に必要であり、Rad52が機能する場にリクルートされてSUMO化を受け、Rad51の一本鎖DNAへのリクルートを部分的に促進し、それとカップルしてRad52の脱SUMO化が起こる制御機構を世界ではじめて提唱した。

Research Products

• [Journal Article] Accumulation of sumoylated Rad52 in checkpoint mutants perturbed in DNA replication (2009)
  • Author(s): Ohuchi, T., Seki, M., Kugou, K., Tada, S., Ohta, K., Enomoto, T.
  • Journal Title: DNA Repair 8 Pages: 690-696
  • Peer Reviewed
• [Journal Article] Generation and characterization of cells that can be conditionally depleted of mitochondrial SOD2 (2009)
  • Author(s): Takada, S., Inoue, E., Tano, K., Yoshii, H., Abe, T., Yoshimura, A., Akita, M., Tada, S., Watanabe, M., Seki, M., Enomoto, T.
  • Journal Title: Biochem.Biophys.Res.Commun. 379(2) Pages: 233-238
  • Peer Reviewed
• [Journal Article] Repression of nascent strand elongation by deregulated Cdt1 during DNA replication in Xenopus egg extracts (2009)
  • Author(s): Tsuyama, T., Watanabe, S., Aoki, A., Cho, Y., Seki, M, Enomoto, T, Tada, S.
  • Journal Title: Mol.Biol.Cell. 20 Pages: 937-947
  • Peer Reviewed
• [Journal Article] Accumulation of sumoylated Rad52 in checkpoint mutants perturbed in DNA replication (2009)
  • Author(s): Ohuchi T, Seki M, Kugou K, Tada S, Ohta K, Enomoto T.
  • Journal Title: DNA Repair (印刷中)
  • Peer Reviewed
• [Journal Article] Repression of nascent strand elongation by deregulated Cdtl during DNA replication in Xenopusegg extracts (2009)
  • Author(s): Tsuyama T, Watanabe S, Aoki A, Cho Y, Seki M, Enomoto T, Tada S.
  • Journal Title: Mol. Biol. Cell 20 Pages: 937-947
  • Peer Reviewed
• [Journal Article] A novel role for Rad17 in homologous recombination (2008)
  • Author(s): Nishino, K., Inoue, E., Takada, S., Abe, T., Akita, M., Yoshimura, A., Tada, S., Kobayashi, M., Yamamoto, K., Seki, M., Enomoto, T.
  • Journal Title: Genes Genet.Syst. 83 Pages: 427-431
  • NAID: 10023888077
  • Peer Reviewed
• [Journal Article] Nuclear localization of Rad52 is pre-requisite for its sumoylation (2008)
  • Author(s): Ohuchi, T., Seki, M., Enomoto, T.
  • Journal Title: Biochem.Biophys.Res.Commun. 372 Pages: 126-130
  • Peer Reviewed
• [Journal Article] Rad52 sumoylation and its involvement in the efficient induction of homologous recombination (2008)
  • Author(s): Ohuchi, T., Seki, M., Branzei, D., Maeda, D., Ui, A., Ogiwara, H., Tada, S., Enomoto, T.
  • Journal Title: DNA Repair 7 Pages: 879-889
  • Peer Reviewed
• [Journal Article] Analyses of functional interaction between RECQL1, RECQL5, and BLM which physically interact with DNA topoisomerase IIIα (2008)
  • Author(s): Otsuki, M., Seki, M., Inoue, E., Abe, T., Narita, Y.,Yoshimura, A., Tada, S., Ishii, Y., Enomoto, T.
  • Journal Title: Biochim.Biophys.Acta 1782 Pages: 75-81
  • Peer Reviewed
• [Journal Article] Vertebrate WRNIP1 and BLM are required for efficient maintenance of genome stability (2008)
  • Author(s): Hayashi, T., Seki, M., Inoue, E.,, Yoshimura, A., Kusa, Y., Tada, S., Enomoto, T.
  • Journal Title: Genes Genet.Syst. 83 Pages: 95-100
  • NAID: 10024395708
  • Peer Reviewed
• [Journal Article] RecQ family helicases in genome stability : lessons from gene disruption studies in DT40 cells (2008)
  • Author(s): Seki, M., Otsuki, M., Ishii, Y., Tada, S., Enomoto, T.
  • Journal Title: Cell Cycle 7 Pages: 2472-2478
• [Journal Article] Nuclear localization of Rad52 is pre-requisite for its sumoylation (2008)
  • Author(s): Ohuchi T, Seki M, Enomoto T.
  • Journal Title: Biochem. Biophys. Res. Commun. 372 Pages: 126-130
  • Peer Reviewed
• [Journal Article] Analyses of functional interaction betweon RECQL1, RECQL5, and BLM which physically interact with DNA topoisomerase IIIα (2008)
  • Author(s): Otsuki M, Seki M, Inoue E, Abe T, Narita Y, Yoshimura A, Tada S, Ishii Y, Enomoto T
  • Journal Title: Biochim. Biophys. Acta. 1782 Pages: 75-81
  • Peer Reviewed
• [Journal Article] Rad52 sumoylation and its involvement in the efficient induction of homologous recombination (2008)
  • Author(s): Ohuchi T, Seki M, Branzei D, Maeda D, Ui A, Ogiwara H, Tada S, Enomoto T.
  • Journal Title: DNA Repair 7 Pages: 879-889
  • Peer Reviewed
• [Journal Article] Analyses of functional interaction between RECQL1, RECQL5, and BLM which physically interact with DNA topoisomerase IIIa (2008)
  • Author(s): Otsuki M, Seki M, Inoue E, Abe T, Narita Y, Yoshimura A, Tada S, Ishii Y, Enomoto T
  • Journal Title: Biochim. Biophys. Acta. 1782 Pages: 75-81
  • Peer Reviewed
• [Journal Article] Functional interactions between BLM and XRCC3 in the cell (2007)
  • Author(s): Otsuki, M., Seki, M., Inoue, E., Yoshimura, A., Kato, G., Yamanouchi, S., Kawabe, Y., Tada, S., Shinohara, A., Komura, J., Ono, T., Takeda, S., Ishii, Y., Enomoto, T.
  • Journal Title: J.Cell Biol. 179 Pages: 53-63
  • Peer Reviewed
• [Journal Article] Ctf18 is required for homologous recombination-mediated double-strand break repair (2007)
  • Author(s): Ogiwara. H., Ohuchi, T,, Ui, A., Tada, S., Enomoto, T., Seki, M.
  • Journal Title: Nucleic Acids Res. 35 Pages: 4989-5000
  • Peer Reviewed
• [Journal Article] Rmi1, a member of the Sgs1-Top3 complex in budding yeast, contributesto sister chromatidcohesion (2007)
  • Author(s): Lai, M.S., Seki, M., Ui, A., Enomoto, T.
  • Journal Title: EMBO Rep. 8 Pages: 685-690
  • Peer Reviewed
• [Journal Article] Possible involvement of RecQL4 in the repair of double-strand DNA breaks in Xenopus egg extracts (2007)
  • Author(s): Kumata Y, Tada S, Yamanada Y, Tsuyama T, Kobayashi T, Dong YP, Ikegami K, Murofushi H, Seki M, Enomoto T.
  • Journal Title: Biochim.Biophys.Acta. 1773 Pages: 556-564
  • Peer Reviewed
• [Journal Article] Activation of a novel pathway involving Mms1 and Rad59 in sgs1 cells (2007)
  • Author(s): Ui A, Seki M, Ogiwara H, Lai MS, Yamamoto K, Tada S, Enomoto T
  • Journal Title: Biochem.Biophys.Res.Commun. 356 Pages: 1031-1037
  • Peer Reviewed
• [Journal Article] WRN functions in a RAD18-dependent damage avoidance pathway (2007)
  • Author(s): Dong, Y.P., Seki, M., Yoshimura, A., Inoue, E., Furukawa, S., Tada, S., Enomoto, T.
  • Journal Title: Biol.Pharm.Bull. 30 Pages: 1080-1083
  • NAID: 110006278395
  • Peer Reviewed
• [Journal Article] Rad50 is involved in MMS-induced recombination between homologous chromosomes in mitotic cells (2007)
  • Author(s): Tomizawa Y, Ui A, Onoda F, Ogiwara H, Tada S, Enomoto T, Seki, M
  • Journal Title: Genes Genet.Syst. 82 Pages: 157-160
  • NAID: 10024394124
  • Peer Reviewed
• [Journal Article] The INO80 chromatin remodeling complex functions in sister chromatid cohesion (2007)
  • Author(s): Ogiwara, H., Enomoto, T., Seki, M.
  • Journal Title: Cell Cycle 6 Pages: 1090-1095
  • Peer Reviewed
• [Journal Article] Actin-related protein Arp4 functions in kinetochore assembly (2007)
  • Author(s): Ogiwara, H., Ui, A., Kawashima, S., Kugou, K., Onoda, F., Iwahashi, H., Harata, M., Ohta, K., Enomoto, T., Seki, M.
  • Journal Title: Nucleic Acids Res. 35 Pages: 3109-3117
  • Peer Reviewed
• [Journal Article] The INO80 complex is required for damage-induced recombination (2007)
  • Author(s): Kawashima, S., Ogiwara, H., Tada, S., Harata, M., Wintersberger, U., Enomoto, T., Seki, M.
  • Journal Title: Biochem.Biophys.Res.Commun. 355 Pages: 835-841
  • Peer Reviewed
• [Journal Article] WRN counteracts the NHEJ pathway upon camptothecin exposure (2007)
  • Author(s): Otsuki, M., Seki, M., Kawabe, Y., Inoue, E., Dong, Y.P., Abe, T., Kato, G., Yoshimura, A., Tada, S., Enomoto, T.
  • Journal Title: Biochem.Biophys.Res.Commun. 355 Pages: 477-482
  • Peer Reviewed
• [Journal Article] Chl1 and Ctf4 are required for damage-induced recombinations (2007)
  • Author(s): Ogiwara, H., Ui, A., Lai, M.S., Enomoto, T, Seki, M.
  • Journal Title: Biochem.Biophys.Res.Commun. 354 Pages: 222-226
  • Peer Reviewed
• [Journal Article] Role of Elg1 protein in double strand break repair (2007)
  • Author(s): Ogiwara, H., Ui, A., Enomoto, T., Seki, M.
  • Journal Title: Nucleic Acids Res 35 Pages: 353-362
  • Peer Reviewed
• [Journal Article] Functional interactions between BLM and XRCC3 in the cell (2007)
  • Author(s): Otsuki M, Seki M, Inoue E, Yoshimura A, Kato G, Yamanouchi S, Kawabe Y, Tada S, Shinohara A, Komura J, Ono T, Takeda S, Ishii Y, Enomoto T.
  • Journal Title: J. Cell Biol. 179 Pages: 53-63
  • Peer Reviewed
• [Journal Article] Ctf18 is required for homologous recombination-mediated double-strand break repair (2007)
  • Author(s): Ogiwara. H., Ohuchi, T, Ui, A., Tada, S., Enomoto, T., Seki, M.
  • Journal Title: Nucleic Acids Res. 35 Pages: 4989-5000
  • Peer Reviewed
• [Journal Article] Rmi1, a member of the Sgs1-Top3 complex in budding yeast, contributesto sister chromatid cohesion (2007)
  • Author(s): Lai, M. S., Seki. M., Ui, A., Enomoto, T.
  • Journal Title: EMBO Rep. 8 Pages: 685-690
  • Peer Reviewed
• [Journal Article] Activation of a novel pathway involving Mms1 and Rad59 in sgs1 cells (2007)
  • Author(s): Ui A, Seki M, Ogiwara H, Lai MS, Yamamoto K, Tada S, Enomoto T.
  • Journal Title: Biochem. Biophys. Res. Commun. 356 Pages: 1031-1037
  • Peer Reviewed
• [Journal Article] Possible involvement of RecQL4 in the repair of double-strand DNA breaks in Xenopus egg extracts (2007)
  • Author(s): Kumata Y, Tada S, Yamanada Y, Tsuyama T, Kobayashi T, Dong YP, Ikegami K, Murofushi H, Seki M, Enomoto T.
  • Journal Title: Biochim. Biophys. Acta. 1773 Pages: 556-564
  • Peer Reviewed
• [Journal Article] WRN counteracts the NHEJ pathway upon camptothecin exposure (2007)
  • Author(s): Otsuki M, Seki M, Kawabe Y, Inoue E, Dong YP, Abe T, Kato G, Yoshimura A, Tada S, Enomoto T.
  • Journal Title: Biochem. Biophys. Res. Commun. 355 Pages: 477-482
  • Peer Reviewed
• [Journal Article] WRN functions in a RAD18-dependent damage avoidance pathway (2007)
  • Author(s): Dong YP, Seki M, Yoshimura A, Inoue E, Furukawa S, Tada S, Enomoto T
  • Journal Title: Biol. Pharm. Bull. 30 Pages: 1080-1083
  • NAID: 110006278395
  • Peer Reviewed
• [Journal Article] Rad50 is involved in MMS-induced recombination between homologous chromosomes in mitotic cells (2007)
  • Author(s): Tomizawa Y, Ui A, Onoda F, Ogiwara H, Tada S, Enomoto T, Seki M.
  • Journal Title: Genes Genet. Syst. 82 Pages: 157-160
  • NAID: 10024394124
  • Peer Reviewed
• [Journal Article] The INO80 chromatin remodeling complex functions in sister chromatid cohesion (2007)
  • Author(s): Ogiwara H, Enomoto T, Seki M.
  • Journal Title: Cell Cycle 6 Pages: 1090-1095
  • Peer Reviewed
• [Journal Article] Actin-related protein Arp4 functions in kinetochore assembly (2007)
  • Author(s): Ogiwara H, Ui A, Kawashima S, Kugou K, Onoda F, Iwahashi H, Harata M, Ohta K, Enomoto T, Seki M.
  • Journal Title: Nucleic Acids Res. 35 Pages: 3109-3117
  • Peer Reviewed
• [Presentation] Takemi Enomoto Requisite conditions for sumoylation of Rad52 and enhancement of the modification in checkpoint mutants (2008)
  • Author(s): Takashi Ohuchi, Masayuki Seki
  • Organizer: 第31回日本分子生物学会年会(第81回日本生化学会大会)合同大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2008-12-12
• [Presentation] Requisite conditions for sumoylation of Rad52 and enhancement of the modification in checkpoint mutants (2008)
  • Author(s): Takashi Ohuchi, Masayuki Seki, Takemi Enomoto
  • Organizer: 第31回日本分子生物学会年会第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸ボートアイランド
  • Year and Date: 2008-12-12
• [Presentation] Requirement of specific amino acids in Rad52 for sumoylation and recombination (2007)
  • Author(s): 関政幸
  • Organizer: 第三回Japan-US DNA Repair Meeting
  • Place of Presentation: 仙台 秋保 ホテル クレセント
  • Year and Date: 2007-05-09
• [Presentation] Requirement of specific amino acids in Rad52 for sumoylation and recombination (2007)
  • Author(s): 関政幸
  • Organizer: 第三回 Japan-US DNA Repair Meeting
  • Place of Presentation: 仙台市/秋保/ホテルク レセント
  • Year and Date: 2007-05-09
• [Book] 蛋白質 核酸 酵素「別冊 ; 染色体サイクル」(Vol.54) (2009)
  • Author(s): 関政幸/Mong Sing Lai/榎本武美
  • Publisher: 共立出版
• [Book] 染色体サイクル (2009)
  • Author(s): 関政幸/Mong Sing Lai/榎本武美
  • Total Pages: 4
  • Publisher: 共立出版