Regulation of homologous recombination through sumoylation of Rad52
Project/Area Number |
19370073
|
Research Category |
Grant-in-Aid for Scientific Research (B)
|
Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Molecular biology
|
Research Institution | Tohoku University |
Principal Investigator |
SEKI Masayuki Tohoku University, 大学院・薬学研究科, 准教授 (70202140)
|
Project Period (FY) |
2007 – 2008
|
Project Status |
Completed (Fiscal Year 2008)
|
Budget Amount *help |
¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000)
Fiscal Year 2008: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000)
Fiscal Year 2007: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000)
|
Keywords | Ubc9 / SUMO / Rad52 / Rad51 / 複製チェックポイント / 相同組換え / SUMO化 / 出芽酵母 / ChIP on chip / チェックポイント / 核移行 |
Research Abstract |
相同組換えの中心酵素Rad52は、複製チャックポイントの欠損株で誘導される通常は活性化しない複製開始点領域に蓄積後、SUMO修飾を受けることを明らかにした。Rad52のSUMO化には、Rad52のオリゴマー形成、核への移行などが事前に必要であり、Rad52が機能する場にリクルートされてSUMO化を受け、Rad51の一本鎖DNAへのリクルートを部分的に促進し、それとカップルしてRad52の脱SUMO化が起こる制御機構を世界ではじめて提唱した。
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Report
(3 results)
Research Products
(44 results)
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[Journal Article] Accumulation of sumoylated Rad52 in checkpoint mutants perturbed in DNA replication2009
Author(s)
Ohuchi, T., Seki, M., Kugou, K., Tada, S., Ohta, K., Enomoto, T.
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Journal Title
DNA Repair 8
Pages: 690-696
Related Report
Peer Reviewed
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[Journal Article] Generation and characterization of cells that can be conditionally depleted of mitochondrial SOD22009
Author(s)
Takada, S., Inoue, E., Tano, K., Yoshii, H., Abe, T., Yoshimura, A., Akita, M., Tada, S., Watanabe, M., Seki, M., Enomoto, T.
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Journal Title
Biochem.Biophys.Res.Commun. 379(2)
Pages: 233-238
Related Report
Peer Reviewed
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[Journal Article] Repression of nascent strand elongation by deregulated Cdt1 during DNA replication in Xenopus egg extracts2009
Author(s)
Tsuyama, T., Watanabe, S., Aoki, A., Cho, Y., Seki, M, Enomoto, T, Tada, S.
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Journal Title
Mol.Biol.Cell. 20
Pages: 937-947
Related Report
Peer Reviewed
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[Journal Article] A novel role for Rad17 in homologous recombination2008
Author(s)
Nishino, K., Inoue, E., Takada, S., Abe, T., Akita, M., Yoshimura, A., Tada, S., Kobayashi, M., Yamamoto, K., Seki, M., Enomoto, T.
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Journal Title
Genes Genet.Syst. 83
Pages: 427-431
NAID
Related Report
Peer Reviewed
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[Journal Article] Rad52 sumoylation and its involvement in the efficient induction of homologous recombination2008
Author(s)
Ohuchi, T., Seki, M., Branzei, D., Maeda, D., Ui, A., Ogiwara, H., Tada, S., Enomoto, T.
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Journal Title
DNA Repair 7
Pages: 879-889
Related Report
Peer Reviewed
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[Journal Article] Analyses of functional interaction between RECQL1, RECQL5, and BLM which physically interact with DNA topoisomerase IIIα2008
Author(s)
Otsuki, M., Seki, M., Inoue, E., Abe, T., Narita, Y.,Yoshimura, A., Tada, S., Ishii, Y., Enomoto, T.
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Journal Title
Biochim.Biophys.Acta 1782
Pages: 75-81
Related Report
Peer Reviewed
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[Journal Article] Vertebrate WRNIP1 and BLM are required for efficient maintenance of genome stability2008
Author(s)
Hayashi, T., Seki, M., Inoue, E.,, Yoshimura, A., Kusa, Y., Tada, S., Enomoto, T.
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Journal Title
Genes Genet.Syst. 83
Pages: 95-100
NAID
Related Report
Peer Reviewed
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[Journal Article] Analyses of functional interaction betweon RECQL1, RECQL5, and BLM which physically interact with DNA topoisomerase IIIα2008
Author(s)
Otsuki M, Seki M, Inoue E, Abe T, Narita Y, Yoshimura A, Tada S, Ishii Y, Enomoto T
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Journal Title
Biochim. Biophys. Acta. 1782
Pages: 75-81
Related Report
Peer Reviewed
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[Journal Article] Analyses of functional interaction between RECQL1, RECQL5, and BLM which physically interact with DNA topoisomerase IIIa2008
Author(s)
Otsuki M, Seki M, Inoue E, Abe T, Narita Y, Yoshimura A, Tada S, Ishii Y, Enomoto T
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Journal Title
Biochim. Biophys. Acta. 1782
Pages: 75-81
Related Report
Peer Reviewed
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[Journal Article] Functional interactions between BLM and XRCC3 in the cell2007
Author(s)
Otsuki, M., Seki, M., Inoue, E., Yoshimura, A., Kato, G., Yamanouchi, S., Kawabe, Y., Tada, S., Shinohara, A., Komura, J., Ono, T., Takeda, S., Ishii, Y., Enomoto, T.
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Journal Title
J.Cell Biol. 179
Pages: 53-63
Related Report
Peer Reviewed
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[Journal Article] Ctf18 is required for homologous recombination-mediated double-strand break repair2007
Author(s)
Ogiwara. H., Ohuchi, T,, Ui, A., Tada, S., Enomoto, T., Seki, M.
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Journal Title
Nucleic Acids Res. 35
Pages: 4989-5000
Related Report
Peer Reviewed
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[Journal Article] WRN functions in a RAD18-dependent damage avoidance pathway2007
Author(s)
Dong, Y.P., Seki, M., Yoshimura, A., Inoue, E., Furukawa, S., Tada, S., Enomoto, T.
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Journal Title
Biol.Pharm.Bull. 30
Pages: 1080-1083
NAID
Related Report
Peer Reviewed
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[Journal Article] Actin-related protein Arp4 functions in kinetochore assembly2007
Author(s)
Ogiwara, H., Ui, A., Kawashima, S., Kugou, K., Onoda, F., Iwahashi, H., Harata, M., Ohta, K., Enomoto, T., Seki, M.
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Journal Title
Nucleic Acids Res. 35
Pages: 3109-3117
Related Report
Peer Reviewed
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[Journal Article] The INO80 complex is required for damage-induced recombination2007
Author(s)
Kawashima, S., Ogiwara, H., Tada, S., Harata, M., Wintersberger, U., Enomoto, T., Seki, M.
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Journal Title
Biochem.Biophys.Res.Commun. 355
Pages: 835-841
Related Report
Peer Reviewed
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[Journal Article] WRN counteracts the NHEJ pathway upon camptothecin exposure2007
Author(s)
Otsuki, M., Seki, M., Kawabe, Y., Inoue, E., Dong, Y.P., Abe, T., Kato, G., Yoshimura, A., Tada, S., Enomoto, T.
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Journal Title
Biochem.Biophys.Res.Commun. 355
Pages: 477-482
Related Report
Peer Reviewed
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[Journal Article] Functional interactions between BLM and XRCC3 in the cell2007
Author(s)
Otsuki M, Seki M, Inoue E, Yoshimura A, Kato G, Yamanouchi S, Kawabe Y, Tada S, Shinohara A, Komura J, Ono T, Takeda S, Ishii Y, Enomoto T.
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Journal Title
J. Cell Biol. 179
Pages: 53-63
Related Report
Peer Reviewed
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[Journal Article] Ctf18 is required for homologous recombination-mediated double-strand break repair2007
Author(s)
Ogiwara. H., Ohuchi, T, Ui, A., Tada, S., Enomoto, T., Seki, M.
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Journal Title
Nucleic Acids Res. 35
Pages: 4989-5000
Related Report
Peer Reviewed
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[Book] 染色体サイクル2009
Author(s)
関政幸/Mong Sing Lai/榎本武美
Total Pages
4
Publisher
共立出版
Related Report