Cell Cycle Regulation of Cell Morphology in Saccharomyces cerevisiae

• Project/Area Number: 19370074
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Molecular biology
• Research Institution: The University of Tokyo
• Principal Investigator: OHYA Yoshikazu The University of Tokyo, 大学院・新領域創成科学研究科, 教授 (20183767)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000); Fiscal Year 2008: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000); Fiscal Year 2007: ¥11,700,000 (Direct Cost: ¥9,000,000、Indirect Cost: ¥2,700,000)
• Keywords: 細胞壁 / 出芽酵母 / 細胞周期チェックポイント / Rho1p / シグナル伝達 / 紡錘極体 / 細胞周期 / HOG / MAP kinase / 紡錘体 / M期

Research Abstract

真核単細胞のモデルである出芽酵母を用いて、「細胞周期進行」という時間軸に添って細胞の形態形成が正しく起きるメカニズムを解明することを本研究の目的にした。そのためには、(1)細胞壁合成などの細胞形態形成がうまく行かない時に細胞周期を停止する機構と、(2)細胞周期進行とともに細胞の形態形成が起きる機構を明らかにすることが必要である。我々は細胞壁合成チェックポンとの研究を行い、複数の核移行の過程がこれに関与することを明らかにした。またG1/S 期のサイクリン依存性タンパク質リン酸化酵素が細胞形態形成の制御因子であるRho1p の活性化に直接影響を与えることを明らかにした。細胞の空間形成は、細胞の数や細胞の機能分化の制御とともに多細胞生物における多細胞集団の構築にとっても極めて重要な要素になっている。したがって、本研究計画で行った細胞周期と「細胞の空間形成」の連携に関する研究は、他の研究との有機的な結合により、真核細胞の運命を決める細胞周期についてより深い理解が得られるものであった。

Research Products

• [Journal Article] Multidimensional quantification of subcellular morphology of Saccharomyces cerevisiae using CalMorph, the high-throughput image-processing program (2009)
  • Author(s): Negishi T, Nogami S, Ohya Y.
  • Journal Title: J Biotechnol 141(3-4) Pages: 109-17
  • Peer Reviewed
• [Journal Article] Novel 24-membered macrolides,JBIR-19 and-20 isolated from Metarhizium sp (2009)
  • Author(s): Kozone I, Ueda J, Watanabe M, Nogami S, Nagai A, Inaba S, Ohya Y, Takagi M, Shin-ya K,
  • Journal Title: fE61. Journal of Antibiotics 62(3) Pages: 159-62
  • Peer Reviewed
• [Journal Article] A microfluidic device to acquire high-magnification microphotographs of yeast cells (2009)
  • Author(s): Ohnuki S, Nogami S, Ohya Y
  • Journal Title: Cell Div 4-5(Epub)
  • Peer Reviewed
• [Journal Article] Comprehensive and quantitative analysis of yeast deletion mutants defective in apical and isotropic bud growth (2009)
  • Author(s): Watanabe M, Watanabe D, Nogami S, Morishita S, Ohya Y.
  • Journal Title: Curr Genet (in press)
  • Peer Reviewed
• [Journal Article] Novel 24-membered macrolides, 313IR-19 and -20 isolated from Metarhiziusp. fE61. (2009)
  • Author(s): Kozone et al.
  • Journal Title: Cell Div 62 Pages: 159-62
  • Peer Reviewed
• [Journal Article] A microfluidic device to acquire high-magnification microphotographs of yast cells. (2009)
  • Author(s): Ohnuki et al.
  • Journal Title: Cell Div. 4 Pages: 5-11
  • Peer Reviewed
• [Journal Article] G1/S cyclin-dependent kinase regulates small GTPase Rho1p through phosphorylation of RhoGEF Tus1p in Saccharomyces cerevisiae (2008)
  • Author(s): Kono K, Nogami S, Abe M, Nishizawa M, Morishita S, Pellman D, Ohya Y
  • Journal Title: Mol Biol Cell 19(4) Pages: 1763-71
  • Peer Reviewed
• [Journal Article] Gl/S cyclin-dependent kinase regulates small GTPase Rholp through phosp horylation of RhoGEF Tuslp in Saccharomyces cerevisiae. (2008)
  • Author(s): Kono et al.
  • Journal Title: Mol Biol Cell 19 Pages: 1763-71
  • Peer Reviewed
• [Journal Article] Diversity of Ca^<2+>-induced morphology revealed by morphological phenotyping of Ca^<2+>-sensitive mutants of Saccharomyces cerevisiae (2007)
  • Author(s): Ohnuki S, Nogami S, Kanai H, Hirata D, Nakatani Y, Morishita S, Ohya Y,
  • Journal Title: Eukaryotic Cell 6(5) Pages: 817-30
  • Peer Reviewed
• [Journal Article] Involvement of small GTPase Rpo1p in cell size control in yeast (2007)
  • Author(s): Kikuchi Y, Mizuuchi E, Nogami S, Morishita S, Ohya Y
  • Journal Title: FEMS Yeast Res 7(4) Pages: 569-78
  • Peer Reviewed
• [Journal Article] Conditional genomic rearrangement by designed meioticrecombination using VDE(PI-SceI) in yeast (2007)
  • Author(s): Fukuda T, Ohya Y, Ohta K
  • Journal Title: Mol Genet Genomics 278(4) Pages: 467-78
  • Peer Reviewed
• [Journal Article] Yvert G, Genetic complexity and QTL mapping of yeast morphological traits (2007)
  • Author(s): Nogami S, Ohya Y
  • Journal Title: PLoS Genet 3(2) Pages: 31-31
  • Peer Reviewed
• [Journal Article] Homologous subunits of yeast 1,3-betaglucan synthase are important for spore wall assembly (2007)
  • Author(s): Ishihara S, Hirata A, Nogami S, BeauvaisA, Latge JP, Ohya Y
  • Journal Title: in Saccharomyces cerevisiae Eukaryotic Cell 6(2) Pages: 143-56
  • Peer Reviewed
• [Journal Article] Diversity of Ca^2+-induced morphology revealed by morphological phenotyping of Ca^2+-sensitive mutants of Saccharomyces cerevisiae (2007)
  • Author(s): Ohnuki S
  • Journal Title: Eukaryotic Cell 6 Pages: 817-30
  • Peer Reviewed
• [Journal Article] Involvement of small GTPase Rholp in cell size control in yeast (2007)
  • Author(s): Kikuchi Y
  • Journal Title: FEMS Yeast Res 7 Pages: 569-78
  • Peer Reviewed
• [Journal Article] Conditional genmic rearrangement by meiotic recombination using VDE (PI-SciI) in yeast (2007)
  • Author(s): Fukuda T
  • Journal Title: Mol Genet Genomics 278 Pages: 467-478
• [Presentation] Quantitative morphological analysis of heterozygous mutants of yeast essential gene (2008)
  • Author(s): 野上識、大矢禎一
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-11
• [Presentation] 出芽酵母の細胞壁チェックポイントにおけるHog1p MAPKリン酸化の役割 (2008)
  • Author(s): 菊地陽、大矢禎一
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-11
• [Presentation] マイクロ流体チップ上における細胞形態の直接観察のための酵母細胞姿勢制御 (2008)
  • Author(s): 大貫慎輔、大矢禎一
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-11
• [Presentation] 高浸透圧に対する1, 3-β-グルカン層の役割に関する超微形態学的研究 (2008)
  • Author(s): 平田愛子、大矢禎一
  • Organizer: 日本植物形態学会第20回大会
  • Place of Presentation: 高知
  • Year and Date: 2008-09-24
• [Presentation] Use of CalMorph for drug discovery (2008)
  • Author(s): Ohya Y
  • Organizer: ISCB2008 29th Annual Conference of the International Society for Clinical Biostatistics
  • Place of Presentation: Copenhagen, Denmark
  • Year and Date: 2008-08-20
• [Presentation] High-throughput microphotography system using microfluidic chip in budding yeast (2008)
  • Author(s): Ohnuki S, Ohya Y
  • Organizer: ISCB2008 29th Annual Conference of the International Society for Clinical Biostatistics
  • Place of Presentation: Copenhagen, Denmark
  • Year and Date: 2008-08-20
• [Presentation] Quantitative morphological analysis of heterozygous mutants of yeast essential gene (2008)
  • Author(s): Nogami S, Ohya Y
  • Organizer: 2008 Yeast Genetics and Molecular Biology Meeting
  • Place of Presentation: Toronto, CANADA
  • Year and Date: 2008-07-24
• [Presentation] Development of high-throughput microphotography system using microfluidic chip for large-scale phnotyping in budding yeast (2008)
  • Author(s): Ohnuki S, Ohya Y
  • Organizer: 2008 Yeast Genetics and Molecular Biology Meeting
  • Place of Presentation: Toronto, CANADA
  • Year and Date: 2008-07-24
• [Presentation] The investigations of roles of yeast karyopherins in coupling cell wall synthesis and cell cycle progression through cell wall integrity checkpoint (2008)
  • Author(s): Negishi T, Ohya Y
  • Organizer: 2008 Yeast Genetics and Molecular Biology Meeting
  • Place of Presentation: Toronto, CANADA
  • Year and Date: 2008-07-24
• [Presentation] Quantitative morphological analysis of yeast cells (2008)
  • Author(s): Ohya Y
  • Organizer: 2008 Yeast Genetics and Molecular Biology Meeting
  • Place of Presentation: Toronto, CANADA
  • Year and Date: 2008-07-24
• [Presentation] 出芽酵母の細胞壁チェックポイントにおけるHOG 経路の役割 (2007)
  • Author(s): 菊地陽、大矢禎一
  • Organizer: 第30回日本分子生物学会年会・第80回日本生化学会大会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-11
• [Presentation] 出芽酵母における大規模表現型解析のためのハイスループット高倍率顕微鏡撮影用マイクロ流体チップの開 (2007)
  • Author(s): 大貫慎輔、大矢禎一
  • Organizer: 第30回日本分子生物学会年会・第80回日本生化学会大会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-11
• [Presentation] Chemical genomics to understand cell morphology of Saccharomyces cerevisiae (2007)
  • Author(s): Ohya Y
  • Organizer: Symposium on Systems Biology Initiative
  • Place of Presentation: 東京
  • Year and Date: 2007-11-18
• [Presentation] Cell wall checkpoint that cell wall integrity in Saccharomyces cerevisiae (2007)
  • Author(s): Ohya Y
  • Organizer: First International Symposium on Plant and Yeast Polysaccharides
  • Place of Presentation: Angret, France
  • Year and Date: 2007-08-26
• [Presentation] Cell wall checkpoint that monitors cell wall integrity in Saccharomyces cerevisiae (2007)
  • Author(s): Ohya Y
  • Organizer: Fisrt Intenational Symposium on Plant and Yeast Polysaccharides
  • Place of Presentation: Angret,France
  • Year and Date: 2007-08-26
• [Presentation] Dynactin and its interacting molecules are involved in cell wall integrity checkpoint of Saccharomyces cerevisiae (2007)
  • Author(s): Ohya Y
  • Organizer: The 2nd International Workshop on Cell Regulations in Division and Arrest
  • Place of Presentation: 沖縄
  • Year and Date: 2007-03-23
• [Presentation] Cell wall integrity checkpoint that monitors cell wall remodeling in Saccharomyces cerevisiae (2007)
  • Author(s): Ohya Y
  • Organizer: The first International Fungal / Plant Cell Wall Meeting
  • Place of Presentation: Biarritz,France
  • Year and Date: 2007-03-12
• [Presentation] 定量的形態情報に基づく出芽酵母のQTL解析定量的形態情報に基づく出芽酵母のQTL解析 (2007)
  • Author(s): 野上識、大矢禎一
  • Organizer: 第30回日本分子生物学会年会・第80回日本生化学会大会
  • Place of Presentation: 横浜
  • Year and Date: 2007-01-11
• [Remarks]
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