High-order Structure-based Molecular Mechanisms in Replicational Initiation Complex Formation, DNA Unwinding, and Dissociation
| Project/Area Number |
19370077
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Molecular biology
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| Research Institution | Kyushu University |
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Principal Investigator |
KATAYAMA Tsutomu Kyushu University, 大学院・薬学研究院, 教授 (70264059)
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| Co-Investigator(Kenkyū-buntansha) |
UEDA Tadashi 九州大学, 大学院・薬学研究院, 教授 (90184928)
ABE Yoshito 九州大学, 大学院・薬学研究院, 准教授 (60315091)
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| Project Period (FY) |
2007 – 2008
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| Project Status |
Completed (Fiscal Year 2008)
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| Budget Amount *help |
¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000)
Fiscal Year 2008: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000)
Fiscal Year 2007: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000)
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| Keywords | DNA 複製開始 / DnaA, 細胞周期 / 蛋白質複合体 / 蛋白質機能構造解析 / ATP / DNA複製 / DnaA / 細胞周期 / タンパク質複合体 / タンパク質機能構造解析 / ヘリカーゼ / DNA動態変化 |
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| Research Abstract |
本研究計画では、特に、高次蛋白質複合体の立体構造と特異機能との連関を基盤として、染色体複製開始の主要な分子機構を解明することを目指した。成果として、大腸菌の複製開始蛋白質DnaA による複製開始複合体の高次構造を推定解析し、DNA 開裂のためキーとなる複製開始複合体の機能構造を同定した。および、DNA 開裂のメカニズムを開始複合体の機能構造と連関させて解明した。さらに、DNA 開裂後におこる、DnaB ヘリカーゼ装着開始機構を解明した。
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Report
(3 results)
Research Products
(100 results)
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[Journal Article] A common mechanism for the ATP-DnaA-dependent formation of open complexes at the replication origin2008
Author(s)
Ozaki, S., Kawakami,H., Nakamura, K., Fujikawa, N., Kagawa, W., Park, S.-Y., Yokoyama, S., Kurumizaka, H., and Katayama, T.
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Journal Title
J. Biol. Chem. 283(13)
Pages: 8351-8362
Related Report
Peer Reviewed
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[Journal Article] Modes of over-initiation, dnaA gene expression and the inhibition of cell division in a novel cold-sensitive hda mutant in Escherichia coli2008
Author(s)
Fujimitsu, K., Su'etsugu, M., Yamaguchi, Y., Mazda, K., Fu, N., Kawakami, H., and Katayama, T.
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Journal Title
J. Bacteriol. 190 (15)
Pages: 5368-5381
Related Report
Peer Reviewed
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[Journal Article] The interaction of DiaA and DnaA regulates the replication cycle in E. coli by directly promoting ATP-DnaA-specific initiation complexes2007
Author(s)
Keyamura, K., Fujikawa, N., Ishida, T., Ozaki, S., Su'etsugu, M., Fujimitsu, K., Kagawa, W., Yokoyama, S., Kurumizaka, H. and Katayama, T.
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Journal Title
Genes Dev. 21
Pages: 2083-2099
Related Report
Peer Reviewed
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[Journal Article] (*Co-corresponding authors) ; Structure and function of DnaA N-terminal domains : Specific sites and mechanisms in inter-DnaA interaction and in DnaB helicase loading on oriC2007
Author(s)
Abe, Y., Jo, T., Matsuda, Y., Matsunaga, C., Katayama, T.*, and Ueda, T.*
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Journal Title
J. Biol. Chem. 282(24)
Pages: 17816-17827
Related Report
Peer Reviewed
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[Journal Article] Assignment of ^1H, ^<13>C and ^<15>N resonances of N-terminal domains of DnaA protein Biomol2007
Author(s)
Abe, Y., Watanabe, N., Yoshida, Y., Ebata, F., Katayama, T., and Ueda, T.
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Journal Title
NMR Assign. 1
Pages: 57-59
Related Report
Peer Reviewed
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[Presentation] Multiple copies of the ribonucleotide reductase genes repress overinitiation of chromosomal replication caused by a novel cold-sensitive hda mutant in Escherichia coli.2008
Author(s)
Fujimitsu, K., Su'etsugu, M., Yamaguchi, Y., Mazda, K., Fu, N., Kawakami H., and Katayama, T.
Organizer
6th 3R (Replication, Recombination, Repair) Symposium
Place of Presentation
Kakegawa, Japan
Related Report
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[Presentation] Analysis on Mechanisms of ATP-DnaA Assembly on oriC and Duplex Unwinding.2008
Author(s)
Katayama, K., Ozaki, S., Keyamura, K., Kawakami, H., Fujikawa, N., Kagawa, W., Nakamura, K., Kazuyuki Fujimitsu, Su'etsugu, M., Yokoyama, S., and Kurumizaka, H.
Organizer
EMBO Workshop on Replication & Segregation of Chromosomes
Place of Presentation
Geilo, Norway
Related Report
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[Presentation] DiaA promotes the unwinding of oriC by directly stimulating the formation of ATP-DnaA- oriC complexes in E. coli.2008
Author(s)
Keyamura, K., Fujikawa, N., Ishida, T., Ozaki, S., Su'etsugu, M., Fujimitsu, K., Kagawa, W., Yokoyama, S., Kurumizaka, H., Katayama, T.
Organizer
EMBO Workshop on Replication & Segregation of Chromosomes
Place of Presentation
Geilo, Norway
Related Report
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[Presentation] Role for specific residues of DnaA AAA+ domain in duplex DNA unwinding during the initiation of chromosomal replication.2007
Author(s)
Ozaki, S., Kawakami, H., Nakamura, K., Fujikawa, N., Kagawa, W., Yokoyama, S., Kurumizaka, H., and Katayama, T.
Organizer
The Seventh International Meeting on AAA Proteins
Place of Presentation
Royal Agricultural College, Cirencester, England
Related Report
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[Presentation] Biochemical analysis of DnaA AAA+domain on the ATP-dependent regulation for the initiation of chromosomal replication.(招待講演)2007
Author(s)
Katayama, T., Ozaki, S., Kawakami, H., Nakamura, K., Keyamura, K., Fujikawa, N., Kagawa, W., Yokoyama, S., and Kurumizaka, H.
Organizer
The Seventh International Meeting on AAA Proteins
Place of Presentation
Royal Agricultural College, Cirencester, England)
Related Report
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