| Budget Amount *help |
¥16,900,000 (Direct Cost: ¥13,000,000、Indirect Cost: ¥3,900,000)
Fiscal Year 2009: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2008: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2007: ¥10,270,000 (Direct Cost: ¥7,900,000、Indirect Cost: ¥2,370,000)
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| Research Abstract |
FilGAP is a newly-identified RhoGAP, which activity is specific for Rac. I have studied the mechanism of regulation of FilGAP activity in vivo. (1) I have identified protein factors, which binds specifically to de-phosphorylated FilGAP. (2) FilGAP is phosphorylated at 6 amino acid residues by ROCK. I have identified Serine 402 as a crucial phosphorylation site to activate FilGAP. (3) The PH domain of FilGAP binds phospholipid PIP3. However, I found that the activity of FilGAP is not dependent on PIP3. Activated small GTPase Arf6 binds the PH domain of FilGAP and stimulates its GAP activity. This suggests FilGAP is involved in the Arf6-dependent regulation of Rac. FilGAP therefore regulates Rac downstream of RhoA and Arf6.
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