The molecular basis of limb regeneration

• Project/Area Number: 19370089
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Developmental biology
• Research Institution: Tohoku University
• Principal Investigator: TAMURA Koji Tohoku University, 大学院・生命科学研究科, 教授 (70261550)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000); Fiscal Year 2008: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000); Fiscal Year 2007: ¥10,270,000 (Direct Cost: ¥7,900,000、Indirect Cost: ¥2,370,000)
• Keywords: 四肢 / 器官再生 / 両生類 / 形態形成 / 遺伝子発現 / 形熊形成 / エピジェネティクス / Hox / トランスジェニック動物 / エンハンサー / 細胞内シグナル伝達

Research Abstract

「器官再生を可能としている分子メカニズムの包括的理解」の研究目的のもと、両生類におけるトランスジェニック技術の再生研究への応用基盤形成を中心に多角的な解析を行った。結果、Prx1エンハンサーを用いたトランスジェニックXenopusの作成により再生幹細胞の可視化に成功しこの形成にMAPK・PI3K経路が必須であることを発見した。また四肢再生過程におけるshh発現にDNAメチル化を含めたエピジェネティックな制御が重要であることを見出した。

Research Products

• [Journal Article] Repatterning in amphibian limb regeneration : a model for study of genetic and epigenetic control of organ regeneration (2009)
  • Author(s): Yakushiji, N., Yokoyama, H., and Tamura, K.
  • Journal Title: Seminars in Cell and Developmental Biology (In Press)
  • Peer Reviewed
• [Journal Article] Normal developmental stages of the Madagascar ground gecko Paroedura pictus with special reference to limb morphogenesis (2009)
  • Author(s): Noro, M., Uejima, A., Abe, G., Manabe, M., and Tamura, K.
  • Journal Title: Developmental Dynamics 238 Pages: 100-109
  • Peer Reviewed
• [Journal Article] Time-lapse analysis reveals local asymmetrical changes in C-looping heart tube (2008)
  • Author(s): Kidokoro, H., Okabe, M.,and Tamura, K.
  • Journal Title: Developmental Dynamics 237 Pages: 3545-3556
  • Peer Reviewed
• [Journal Article] Competent stripes for diverse positions of limbs/fins in gnathostome embryos (2008)
  • Author(s): Yonei-Tamura, S., Abe, G., Tanaka, Y., Anno, H., Noro, M., Ide, H., Aono, H., Kuraishi, R., Osumi, N., Kuratani, S.,and Tamura, K.
  • Journal Title: Evolution & Development 10 Pages: 737-745
  • Peer Reviewed
• [Journal Article] Non-canonical Role of Hox14 revealed by its expression patterns in lamprey and shark (2008)
  • Author(s): Kuraku, S., Takio, Y., Tamura, K.., Aono, H., Meyer, A., Kuratani, S.
  • Journal Title: PNAS 105 Pages: 6679-6683
  • Peer Reviewed
• [Journal Article] The autopod : its formation during limb development (2008)
  • Author(s): Tamura, K.., Yonei-Tamura, S., Yano, T., Yokoyama, H.,and Ide, H.
  • Journal Title: Development Growth and Differentiation 50 Suppl 1
  • NAID: 10024399869
  • Peer Reviewed
• [Journal Article] Mitf contributes to melanosome distribution and melanophore dendricity (2008)
  • Author(s): Kawasaki, A., Kumasaka, M., Satoh, A., Suzuki, M., Tamura, K.., Goto, T., Asashima, M.,and Yamamoto, H.
  • Journal Title: Pigment Cell and Melanoma Research 21 Pages: 56-62
  • Peer Reviewed
• [Journal Article] Competent stripes for diverse positions of limbs / fins in gnathostome embryos (2008)
  • Author(s): Yonei-Tamura, S., et.al.
  • Journal Title: Evolution & Development 10 Pages: 737-745
  • Peer Reviewed
• [Journal Article] Correlation between Shh expression and DNA methylation status of the limb-specific Shh enhancer region during limb regeneration in amphibians (2007)
  • Author(s): Yakushiji, N., Suzuki, M., Satoh, A., Sagai, T., Shiroishi, T., Kobayashi, H., Sasaki, H., Ide, H.,and Tamura, K.
  • Journal Title: Developmental Biology 312 Pages: 171-182
  • Peer Reviewed
• [Journal Article] Essential role of heparan sulfate 2-O-sulfotransferase in chick limb bud patterning and development (2007)
  • Author(s): Kobayashi, T., Habuchi, H., Tamura, K.., Ide, H., Kimata, K.
  • Journal Title: Journal of Biological Chemistry 282 Pages: 19589-19597
  • Peer Reviewed
• [Journal Article] Transgenic Xenopus with prx1 limb enhancer reveals crucial contribution of MEK/ERK and PI3K/AKT pathways in blastema formation during limb regeneration (2007)
  • Author(s): Suzuki, M., Satoh, A., Ide, H.,and Tamura, K.
  • Journal Title: Developmental Biology 304 Pages: 675-686
  • Peer Reviewed
• [Journal Article] Specification of cell fate along the proximal-distal axis in the developing chick limb bud (2007)
  • Author(s): Sato, K., Koizumi, Y., Takahashi, M., Kuroiwa, A.,and Tamura, K.
  • Journal Title: Development 134 Pages: 1397-1406
  • Peer Reviewed
• [Journal Article] Function of FGF signaling in the developmental process of the median fin fold in zebrafish (2007)
  • Author(s): Abe, G., Ide, H.,and Tamura, K.
  • Journal Title: Developmental Biology 304 Pages: 355-366
  • Peer Reviewed
• [Journal Article] Correlation between Shh expression and DNA methylation status of the limb-specific Shh enhancer region during limb regeneration in amphibians. (2007)
  • Author(s): Yakushiji, N., Suzuki, M., Satoh, A., Sagai, T., Shiroishi, T., Kobayashi, H., Sasaki, H., Ide, H., and Tamura, K.
  • Journal Title: Developmental Biology 312 Pages: 171-182
  • Peer Reviewed
• [Journal Article] Specification of cell fate along the proximal-distal axis in the developing chick limb bud., (2007)
  • Author(s): Sato, K., Koizumi, . Y., Takahashi, M., Kuroiwa, A., and Tamura, K.
  • Journal Title: Development 134 Pages: 1397-1406
  • Peer Reviewed
• [Journal Article] Transgenic Xenopus with prx1 limb enhancer reveals crucial contribution of MEK/ERK and PI3K/aKT pathways in blastem a formation during limb regeneration. (2007)
  • Author(s): Suzuki, M., Satoh, A., Ide, H., and Tamura, K.
  • Journal Title: Developmental Biology 304 Pages: 675-686
  • Peer Reviewed
• [Presentation] A Variety of Limb Regene-ration Abilities in Tetrapods (2008)
  • Author(s): 田村宏治
  • Organizer: 第8回NIBB-EMBL合同シンポジウム"Evolution : Genomes, cell types and shapes"
  • Place of Presentation: 岡崎コンファレンスセンター
  • Year and Date: 2008-11-23
• [Presentation] Genetic and Epigenetic Controls of Gene Expression During Limb Regeneration In Xenopus (2008)
  • Author(s): 田村宏治、薬師寺那由他
  • Organizer: 第16回CDBミーティング"Cis-sequence Regulation and its Evolu-tion"
  • Place of Presentation: 理化学研究所発生・再生科学総合研究センター
  • Year and Date: 2008-10-01
• [Presentation] アフリカツメガエル四肢再生過程における基部先端部軸形成不全の分子メカニズム (2008)
  • Author(s): 大湖史朗、薬師寺那由他、横山仁、田村宏治
  • Organizer: 日本動物学会第79回大会
  • Place of Presentation: 福岡大学七隈キャンパス
  • Year and Date: 2008-09-05
• [Presentation] Molecular and cellular mechanisms of pattern-deficient limb regeneration in Xenopus froglet (2008)
  • Author(s): 田村宏治、薬師寺那由他、大湖史朗、横山仁
  • Organizer: 10th International Conference on Limb Development and Regeneration(Teatro Auditorio/Euroforum Infantes, San Lorenzo de El Escorial)
  • Place of Presentation: Madrid (Spain)
  • Year and Date: 2008-08-16
• [Presentation] Molecular and cellular mechanisms of pattern-deficient limb regeneration in Xenopus froglet (2008)
  • Author(s): 田村宏治、薬師寺那由他、大湖史朗、横山仁
  • Organizer: 10th International Conference on Limb Development and Regeneration
  • Place of Presentation: Teatro Auditorio / Euro-forum Infantes, Madrid
  • Year and Date: 2008-08-16
• [Presentation] アフリカツメガエルにおける皮膚創傷治癒過程-付加再生との比較の観点から- (2008)
  • Author(s): 丸岡玉枝、薬師寺那由他、大湖史朗、横山仁、田村宏治
  • Organizer: 平成20年度日本動物学会東北支部大会
  • Place of Presentation: 盛岡市観光文化交流センター
  • Year and Date: 2008-07-26
• [Presentation] Reactivation of SHH signaling is a key to anterior-posterior(AP)repatterning during Xenopus froglet limb regeneration (2008)
  • Author(s): 藥師寺那由他、嵯峨井知子、城石俊彦、小林久人、佐々木裕之、井出宏之、田村宏治
  • Organizer: 日本発生生物学会第41回大会(ISDB共催)ワークショップ
  • Place of Presentation: 徳島県郷土文化会館
  • Year and Date: 2008-05-30
• [Presentation] Patterning-deficient limb regeneration in Xenopus (2008)
  • Author(s): 田村宏治、藥師寺那由他、大湖史朗、横山仁
  • Organizer: 日本発生生物学会第41回大会(ISDB共催)シンポジウム
  • Place of Presentation: 徳島県郷土文化会館
  • Year and Date: 2008-05-28
• [Presentation] 四肢再生過程におけるShhの発現を制御するエピジェネティクス機構 (2008)
  • Author(s): 藥師寺那由他、嵯峨井知子、城石俊彦、小林久人、佐々木裕之、井出宏之、田村宏治
  • Organizer: 第2回日本エピジェネティクス研究会年会
  • Place of Presentation: 東レ総合研修センター
  • Year and Date: 2008-05-10
• [Presentation] 四肢発生メカニズムから見た有対付属肢の相同性と多様性 (2008)
  • Author(s): 田村宏治
  • Organizer: 第113回日本解剖学会総会・全国学術集会シンポジウム
  • Place of Presentation: 大分大学医学部
  • Year and Date: 2008-03-27
• [Presentation] Reactivation of SHH signaling is a key to anterior-posterior(AP)repatterning during Xenopus froglet limb regeneration (2008)
  • Author(s): 藥師寺那由他、井出宏之、田村宏治
  • Organizer: The EMBO Conference on the Molecular and Cellular Basis of Regeneration and Tissue Repair
  • Place of Presentation: Caixa Forum, Palma de Mallo-rca, Spain
• [Presentation] 両生類四肢再生にみる器官再生の基礎 (2007)
  • Author(s): 田村宏治
  • Organizer: 第16回日本形成外科学会基礎学術集会
  • Place of Presentation: クラウンプラザ神戸
  • Year and Date: 2007-10-11
• [Presentation] 両生類における四肢再生の鍵となるShh発現のエピジェネティクス制御 (2007)
  • Author(s): 藥師寺那由他、嵯峨井知子、城石俊彦、小林久人、佐々木裕之、井出宏之、田村宏治
  • Organizer: 第78回日本動物学会
  • Place of Presentation: 弘前大学文京町キャンパス
  • Year and Date: 2007-09-20
• [Presentation] Epigenetic regulation of Shh expression as a key for limb regeneration in amphibians (2007)
  • Author(s): 田村宏治
  • Organizer: The Healing Foundation Centre for Tissue Regeneration Opening Symposium
  • Place of Presentation: The University of Manchester
  • Year and Date: 2007-09-14
• [Presentation] Epigenetic regulation of Shh expression as a key for limb regeneration in amphibians. (2007)
  • Author(s): Tamura, K.
  • Organizer: The Healing Foundation Centre for Tissue Regeneration Opening Symposium
  • Place of Presentation: The University of Manchester
  • Year and Date: 2007-09-14
• [Presentation] ツメガエル四肢再生過程における基部先端部軸形成不全の分子メカニズム (2007)
  • Author(s): 大湖史朗、伊藤あかり、鈴木誠、佐藤伸、井出宏之、田村宏治
  • Organizer: 日本動物学会平成19年度東北支部大会
  • Place of Presentation: カレッジプラザ、秋田
  • Year and Date: 2007-07-28
• [Presentation] Conserved developmental mechanism of vertebrate appendages (2007)
  • Author(s): 田村宏治
  • Organizer: 8th International Congress of Vertebrate Morphology
  • Place of Presentation: the University P. & M. Curie(UPMC Paris VI)
  • Year and Date: 2007-07-18
• [Presentation] 両生類における四肢再生の鍵となるShh発現のエピジェネティクス制御 (2007)
  • Author(s): 藥師寺那由他、鈴木誠、佐藤伸、嵯峨井知子、城石俊彦、小林久人、佐々木裕之、井出宏之、田村宏治
  • Organizer: 第1回日本エピジェネティクス研究会年会
  • Place of Presentation: 大阪大学コンベンションセンター
  • Year and Date: 2007-06-15
• [Presentation] Epigenetic regulation plays a key role in gene re-expression during Xenopus limb regeneration (2007)
  • Author(s): 藥師寺那由他、嵯峨井知子、城石俊彦、小林久人、佐々木裕之、井出宏之、田村宏治
  • Organizer: 第40回日本発生生物学会
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2007-05-28
• [Presentation] Epigenetic Regulation of Shh Expression as a Key for Limb Regeneration in Amphibians (2007)
  • Author(s): 田村宏治
  • Organizer: NIBB-EMBL合同シンポジウムCell & Developmental Biology
  • Place of Presentation: 基礎生物学研究所
  • Year and Date: 2007-05-24
• [Book] 6章四肢の進化と発生.21世紀の動物科学シリーズ3.「動物の形態進化のメカニズム」(社団法人日本動物学会監修) (2007)
  • Author(s): 田村宏治、米井小百合
  • Publisher: 培風館
• [Book] Intermission 3 体節筋の発生と進化.21世紀の動物科学シリーズ3.「動物の形態進化のメカニズム」(社団法人日本動物学会監修) (2007)
  • Author(s): 日下部りえ、田村宏治、倉谷滋
  • Publisher: 培風館
• [Book] 21世紀の動物科学シリーズ3.「動物の形態進化のメカニズム」 (2007)
  • Author(s): 田村宏治、米井小百合
  • Total Pages: 244
  • Publisher: 培風館
• [Remarks] 「研究ノート拝見生物の設計図を解く」河北新報2007.07.24朝刊18面(研究紹介記事)
• [Remarks] 田村宏治.脊椎動物の進化と発生の関係(あなたの背中に肢は生えるか?).「東北大学学部・学科説明会」模擬講義.2008.12.05宮城県仙台第一高等学校
• [Remarks] 田村宏治.動物四肢の発生と進化.栃木県立宇都宮高等学校生徒向け講演(スーパー・サイエンス・ハイスクール事業).2008.03.18.栃木県立宇都宮高等学校
• [Remarks]
  • URL: http://www.biology.tohoku.ac.jp/lab-www/tamlab/