| Budget Amount *help |
¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
Fiscal Year 2009: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2008: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2007: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
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| Research Abstract |
We investigated the molecular mechanism of the role of a nuclear membrane protein, Nemp1, in gene regulation during early Xenopus eye development. We found that Nemp1 is localized to the inner nuclear membrane with region B facing the nucleoplasm, and that Nemp1 binds to Barrier-to-Autointegration Factor (BAF) and the GTP form of the small GTPase Ran. Nemp1 inhibits activation of a rax enhancer reporter gene by Otx2 in a region B-dependent manner. These data lead to the possibility that Nemp1 is involved in the expression of the eye-specific gene rax by functioning at the inner nuclear membrane through interactions with BAF and Ran.
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