Analysis of gene regulation and eye development by a novel nuclear protein, Nemp1
Project/Area Number |
19370091
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Developmental biology
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Research Institution | The University of Tokyo |
Principal Investigator |
TAIRA Masanori The University of Tokyo, 大学院・理学系研究科, 准教授 (60150083)
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Project Period (FY) |
2007 – 2009
|
Project Status |
Completed (Fiscal Year 2009)
|
Budget Amount *help |
¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
Fiscal Year 2009: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2008: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2007: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
|
Keywords | 初期発生 / 眼形成 / 核内膜蛋白質 / 遺伝子発現調節 / otx2 / rax / BAF / Ran / アフリカツメガエル / 眼胞形成 / 核膜蛋白質 / 核内輸送 / Nempl / Otx2 / クロマチン蛋白質 / Nemp1 / BAF結合配列 |
Research Abstract |
We investigated the molecular mechanism of the role of a nuclear membrane protein, Nemp1, in gene regulation during early Xenopus eye development. We found that Nemp1 is localized to the inner nuclear membrane with region B facing the nucleoplasm, and that Nemp1 binds to Barrier-to-Autointegration Factor (BAF) and the GTP form of the small GTPase Ran. Nemp1 inhibits activation of a rax enhancer reporter gene by Otx2 in a region B-dependent manner. These data lead to the possibility that Nemp1 is involved in the expression of the eye-specific gene rax by functioning at the inner nuclear membrane through interactions with BAF and Ran.
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Report
(4 results)
Research Products
(44 results)
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[Journal Article] The RNA binding protein Mex3b has a fine-tuning system for mRNA regulation in early Xenopus development2009
Author(s)
Takada, H., Kawana, T., Ito, Y., Kikuno, R. F., Mamada, H., Araki, T., Koga, H., Asashima, M., Taira M.
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Journal Title
Development 136
Pages: 2413-2422
Related Report
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[Journal Article] Evolutionary origins of blastoporal expression and organizer activity of the vertebrate gastrula organizer gene lhxl and its ancient metazoan paralog lhx32009
Author(s)
Yasuoka, Y., Kobayashi, M., Kurokawa, D., Akasaka, K., Saiga, H., Taira M.
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Journal Title
Development 136
Pages: 2005-2014
Related Report
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[Journal Article] The RNA binding protein Mex3b has a fine-tuning system for mRNA regulation in early Xenopus development2009
Author(s)
Takada, H., Kawana, T., Ito, Y., Kikuno, R.F., Mamada, H., Araki, T., Koga, H., Asashima, M., Taira, M.
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Journal Title
Development
Volume: 136
Pages: 2413-2422
Related Report
Peer Reviewed
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[Journal Article] Evolutionary roots of blastoporal expression and 'organizer' activity of the vertebrate gastrula organizer gene lhx1 and its ancient metazoan paralog lhx32009
Author(s)
Yasuoka, Y, Kobayashi, M., Kurokawa, D., Akasaka, K., Saiga, H., Taira, M
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Journal Title
Development
Volume: 136
Pages: 2005-2014
Related Report
Peer Reviewed
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[Journal Article] The keratin-related Ouroboros proteins function as immune antigens mediating tail regression in Xenopus metamorphosis2009
Author(s)
Mukaigasa, K., Hanasaki, A., Maeno, M., Fujii, H., Hayashida, S.-i., Itoh, M., Kobayashi, M., Tochinai, S., Hatta, M., Iwabuchi, K., Taira, M., Onoe, K., Izutsu, Y.
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Journal Title
Proc.Natl.Acad.Sci.USA
Volume: 106
Pages: 118309-18314
Related Report
Peer Reviewed
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[Journal Article] Inaugural Article: A comparative analysis of frog early development2007
Author(s)
del Pine, E. M., Venegas-Ferrin, M., Romero-Carvajal, A., Montenegro-Larrea, P., Saenz-Ponce, N., Moya, I. M., Alarcon, I., Sudou, N., Yamamoto, S., Taira M.
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Journal Title
Proc.Natl.Acad.Sci.USA 104
Pages: 11882-11888
Related Report
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[Journal Article] Recombinant To12 transposase with activity in Xenopus embryos2007
Author(s)
Shibano, T., Taira M., Suetake, I., Kawakami, K., Asashima, M., Tajima, S., Taira M.
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Journal Title
FEBS Lett 581
Pages: 4333-4336
Related Report
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