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Roles of membrane microdomains in fertilization and activation of development

Research Project

Project/Area Number 19370094
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Developmental biology
Research InstitutionKyoto Sangyo University

Principal Investigator

SATO Ken-ichi  Kyoto Sangyo University, 工学部, 教授 (30235337)

Project Period (FY) 2007 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000)
Fiscal Year 2009: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2008: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2007: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Keywords受精 / シグナル伝達 / 細胞膜マイクロドメイン / Src / ウロプラキンIII / 初期発生 / 生殖細胞 / 配偶子間相互作用 / 卵活性化 / モデル生物
Research Abstract

On uroplakin III (UPIII), its mechanism for localization to the membrane microdomain and for negative regulation of Src. 2)Phosphorylation of hnRNP K and its physiological relevance to translational control of maternal mRNAs. 3) PI3-kinase and Akt as important components for fertilization signaling. 4)Molecular function of ARISX as a carbohydrate-containing protein. 5)Molecular mechanism of an enzymatic regulation of phospholipase Cζ, a sperm factor in mammals, that involves partial proteolysis. 6) Importance of tyrosine phosphorylation of Stat3 in human melanoma cell lines.

Report

(4 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • 2007 Annual Research Report
  • Research Products

    (62 results)

All 2010 2009 2008 2007 Other

All Journal Article (16 results) (of which Peer Reviewed: 16 results) Presentation (40 results) Book (2 results) Remarks (4 results)

  • [Journal Article] Analysis of signal transduction in cell-free extracts and rafts of Xenopus eggs.2010

    • Author(s)
      Tokmakov AA, Iwasaki T, Sato K, Fukami Y.
    • Journal Title

      Methods Jan 13

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Analysis of signal transduction in cell-free extracts and rafts of Xenopus eggs2010

    • Author(s)
      Tokmakov AA, Iwasaki T, Sato K, Fukami Y
    • Journal Title

      Methods(掲載決定) (未定)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Evidence that phosphatidylinositol 3-kinase is involved in sperm-induced tyrosine kinase signaling in Xenopus egg fertilization.2009

    • Author(s)
      Mammadova G, Iwasaki T, Tokmakov AA, Fukami Y, Sato K.
    • Journal Title

      BMC Dev Biol 9

      Pages: 68-68

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] 12-O-tetradecanoylphorbol-13-acetae inhibits melanoma growth by inactivation of STAT3 through protein kinase C-activated tyrosine phosphatase(s).2009

    • Author(s)
      Oka M, Sumita N, Sakaguchi M, Iwasaki T, Bito T, Kageshita T, Sato K, Fukami Y, Nishigori C.
    • Journal Title

      J. Biol. Chem 284

      Pages: 30416-30423

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Evidence that phosphatidylinositol 3-kinase is involved in sperm-induced tyrosine kinase signaling in Xenopus egg fertilization2009

    • Author(s)
      Mammadova G, Iwasaki T, Tokmakov AA, Fukami Y, Sato K
    • Journal Title

      BioMedCentral Developmental Biology 9

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 12-O-tetradecanoylphorbol-13-acetate inhibits melanoma growth by inactivation of STAT3 through protein kinase C-activated tyrosine phosphatase(s)2009

    • Author(s)
      Oka M, Sumita N, Sakaguchi M, Iwasaki T, Bito T, Kageshita T, Sato K, Fukami Y, Nishigori C
    • Journal Title

      Journal of Biological Chemistry 284

      Pages: 30416-30423

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Signal transduction of fertilization in frog eggs and anti-apoptotic mechanism of human cancer cells: common and specific function of membrane microdomains2008

    • Author(s)
      Sato K.
    • Journal Title

      Open Biochem J. 2

      Pages: 49-59

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Expression, phosphorylation, and mRNA-binding of heterogeneous nuclear ribonucleo-protein K in Xenopus oocytes, eggs, and early embryos.2008

    • Author(s)
      Iwasaki T, Koretomo Y, Fukuda T, Paronetto MP, Sette C, Fukami Y, Sato K.
    • Journal Title

      Dev Growth Differ 50

      Pages: 23-40

    • NAID

      10020274182

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Signal transduction of fertilization in frog eggs and anti-apoptotic mechanism of human cancer cells : common and specific function of membrane microdomains2008

    • Author(s)
      Sato, K.
    • Journal Title

      The Open Biochemistry Journal 2

      Pages: 49-59

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Expression, phosphorylation, and mRNA-binding of heterogeneous nuclear ribonucleoprotein K in Xenopus oocytes, eggs, and early embryos2008

    • Author(s)
      Iwasaki, T.
    • Journal Title

      Development Growth & Differentiation 50

      Pages: 23-40

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Proteolytic processing of phospholipase Cζand [Ca2+]i oscillations during mammalian fertilization.2007

    • Author(s)
      Kurokawa M, Yoon SY, Alfandari D, Fukami K, Sato K, Fissore RA
    • Journal Title

      Dev Biol 312

      Pages: 407-418

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Analysis of terminal sugar moieties and species-specificities of acrosome reaction-inducing substance in Xenopus (ARISX).2007

    • Author(s)
      Ueda Y, Imaizumi C, Kubo H, Sato K, Fukami Y, Iwao Y.
    • Journal Title

      Dev Growth Differ 49

      Pages: 591-601

    • NAID

      10019748367

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of Xenopus egg membrane microdomains containing uroplakin Ib/III complex: roles of their molecular interactions for subcellular localization and signal transduction.2007

    • Author(s)
      Mahbub Hasan AK, Ou Z, Sakakibara K, Hirahara S, Iwasaki T, Sato K, Fukami Y.
    • Journal Title

      Genes Cells 12

      Pages: 251-267

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Proteolytic processing of phospholipase Cζ and [Ca^<2+>]i oscillations during mammalian fertilization2007

    • Author(s)
      Kurokawa, M.
    • Journal Title

      Developmental Biology 312

      Pages: 407-418

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Analysis of terminal sugar moieties and species-specificities of acrosome reaction-inducing substance in Xenopus(ARISX)2007

    • Author(s)
      Ueda, Y.
    • Journal Title

      Development Growth & Differentiation 49

      Pages: 591-601

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of Xenopus egg membrane microdomains containing uroplakin Ib/III complex: roles of their molecular interactions for subcellular localization and signal transduction2007

    • Author(s)
      Mahbub Hasan, AK.
    • Journal Title

      Genes to Cells 12

      Pages: 251-67

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] 受精卵およびがん細胞におけるチロシンリン酸化シグナル伝達機構2010

    • Author(s)
      佐藤賢一
    • Organizer
      神戸大学研究環重点研究チーム学術講演会
    • Place of Presentation
      神戸・神戸大学
    • Year and Date
      2010-03-12
    • Related Report
      2009 Final Research Report
  • [Presentation] Analysis of gamete interaction and signal transduction in Xenopus laevis by using isolated egg membrane microdomains2009

    • Author(s)
      佐藤賢一
    • Organizer
      iEMD、日本分子生物学会年会ワークショップ
    • Place of Presentation
      横浜・パシフィコ横浜
    • Year and Date
      2009-12-10
    • Related Report
      2009 Final Research Report
  • [Presentation] Roles of membrane microdomains in Xenopus egg fertilization2009

    • Author(s)
      Sato K
    • Organizer
      Gordon Research Conference on "Fertilization and Activation of Development"
    • Place of Presentation
      アメリカ、ニューハンプシャー州、プリマス
    • Related Report
      2009 Annual Research Report
  • [Presentation] ブタ卵細胞質内精子注入における精子処理が精子内PhospholipaseCζ(PLCζ)量を減少させ卵活性化誘起能低下を引き起こす2009

    • Author(s)
      中井美智子、伊藤潤哉、佐藤賢一、野口純子、金子浩之、柏崎直巳、菊地和弘
    • Organizer
      第102回日本繁殖生物学会
    • Place of Presentation
      奈良、近畿大学
    • Related Report
      2009 Annual Research Report
  • [Presentation] アフリカツメガエル卵黄チロシンリン酸化タンパク質pp33の性状解析2009

    • Author(s)
      玖島将太、ママドワギュナイ、深見泰夫、佐藤賢一
    • Organizer
      日本動物学会
    • Place of Presentation
      静岡、静岡グランシップ
    • Related Report
      2009 Annual Research Report
  • [Presentation] Involvement of Phosphatidylinositol 3-kinase in sperm-induced tyrosine kinase signaling in Xenopus egg fertilization2009

    • Author(s)
      Gunay Mammadova, Shota Kushima, Tetsushi Iwasaki, Alexander A.Tokmakov, Yasuo Fukami, Ken-ichi Sato
    • Organizer
      日本動物学会
    • Place of Presentation
      静岡、静岡グランシップ
    • Related Report
      2009 Annual Research Report
  • [Presentation] TPA inhibits melanoma growth by inactivation of STAT3 through PKC-activated tyrosine phosphatase(s)2009

    • Author(s)
      岡昌宏、尾藤利憲、佐藤賢一、深見泰夫、錦織千佳子
    • Organizer
      第68回日本癌学会学術総会
    • Place of Presentation
      横浜、パシフィコ横浜など
    • Related Report
      2009 Annual Research Report
  • [Presentation] 配偶子間相互作用と発生開始のシグナル伝達:単離した卵細胞膜マイクロドメイン(iEMD)を用いた解析2009

    • Author(s)
      佐藤賢一、玖島将太
    • Organizer
      第3回日本ツメガエル研究集会
    • Place of Presentation
      広島、宮島杜の宿
    • Related Report
      2009 Annual Research Report
  • [Presentation] Analysis of Xenopus Src and G protein functions in egg activation2009

    • Author(s)
      A.K.M.Mahbub Hasan、岩崎哲史、ママドワギュナイ、井口将、トクマコフアレクサンダーA.、佐藤賢一、深見泰夫
    • Organizer
      第82回日本生化学会大会
    • Place of Presentation
      神戸、神戸国際展示場など
    • Related Report
      2009 Annual Research Report
  • [Presentation] 単離した卵細胞膜マイクロドメイン(iEMD)を用いた配偶子間相互作用と受精シグナリング機構の解析2009

    • Author(s)
      佐藤賢一、玖島将太、ママドワギュナイ、マブブハサンAKM、深見泰夫
    • Organizer
      第32回日本分子生物学会年会
    • Place of Presentation
      横浜、パシフィコ横浜
    • Related Report
      2009 Annual Research Report
  • [Presentation] ツメガエル卵受精シグナル伝達におけるホスファチジルイノシトール3キナーゼの関与2009

    • Author(s)
      ママドワギュナイ、岩崎哲史、トクマコプアレクサンデル、深見泰夫、佐藤賢一
    • Organizer
      第32回日本分子生物学会年会
    • Place of Presentation
      横浜、パシフィコ横浜
    • Related Report
      2009 Annual Research Report
  • [Presentation] 単離した細胞膜マイクロドメインiEMD(isolated egg membrane microdomains)を用いた新しい受精関連分子の同定2009

    • Author(s)
      玖島将太、佐藤賢一
    • Organizer
      第32回日本分子生物学会年会
    • Place of Presentation
      横浜、パシフィコ横浜
    • Related Report
      2009 Annual Research Report
  • [Presentation] ヒトがん細胞において血清飢餓環境下でSrc依存的に発現制御を受ける遺伝子の解析2009

    • Author(s)
      河田有佳里、山本夏美、深見泰夫、佐藤賢一
    • Organizer
      第32回日本分子生物学会年会
    • Place of Presentation
      横浜、パシフィコ横浜
    • Related Report
      2009 Annual Research Report
  • [Presentation] ツメガエル卵カスパーゼ活性の性状解析2009

    • Author(s)
      松本尚士、玖島将太、佐藤賢一
    • Organizer
      第32回日本分子生物学会年会
    • Place of Presentation
      横浜、パシフィコ横浜
    • Related Report
      2009 Annual Research Report
  • [Presentation] ヒトがん細胞ウロプラキンファミリータンパク質の性状解析2009

    • Author(s)
      太田晃奈、久富利恵、奥成祥、真田昌幸、淺田智久、玖島将太、佐藤賢一
    • Organizer
      第32回日本分子生物学会年会
    • Place of Presentation
      横浜、パシフィコ横浜
    • Related Report
      2009 Annual Research Report
  • [Presentation] ゼノパス初期発生におけるhnRNP K結合mRNAの発現解析2009

    • Author(s)
      Tetsushi Iwasaki, A.K.M.Mahbub Hasan, Sho Iguchi, Gunay Mammadova, Alexander A.Tokmakov, Ken-ichi Sato, Yasuo Fukami
    • Organizer
      第32回日本分子生物学会年会
    • Place of Presentation
      横浜、パシフィコ横浜
    • Related Report
      2009 Annual Research Report
  • [Presentation] Study of the G protein function in Xenopus fertilization2009

    • Author(s)
      A.K.M.Mahbub Hasan, Tetsushi Iwasaki, Alexander A.Tokmakov, Ken-ichi Sato, Yasuo Fukami
    • Organizer
      第32回日本分子生物学会年会
    • Place of Presentation
      横浜、パシフィコ横浜
    • Related Report
      2009 Annual Research Report
  • [Presentation] Membrane microdomain-associated uroplakin III is involved in serum-independent growth and survival of human bladder carcinom2008

    • Author(s)
      佐藤賢一
    • Organizer
      第81回日本生化学会大会・第31回日本分子生物学会年会・合同大会(BMB2008)
    • Place of Presentation
      神戸
    • Year and Date
      2008-12-09
    • Related Report
      2008 Annual Research Report
  • [Presentation] Involvement of heterotrimeric G protein(s)in Xenopus egg activation signaling2008

    • Author(s)
      岩崎哲史
    • Organizer
      第81回日本生化学会大会・第31回日本分子生物学会年会・合同大会(BMB2008)
    • Place of Presentation
      神戸
    • Year and Date
      2008-12-09
    • Related Report
      2008 Annual Research Report
  • [Presentation] Characterization of a 33-kDa yolk platele-associated protein in Xenopus oocytes that is highly and stably phosphorylated on2008

    • Author(s)
      Mammadova Gunay
    • Organizer
      第81回日本生化学会大会・第31回日本分子生物学会年会・合同大会(BMB2008)
    • Place of Presentation
      神戸
    • Year and Date
      2008-12-09
    • Related Report
      2008 Annual Research Report
  • [Presentation] Roles of membrane microdomain-associated Src and uroplakin III in fertilization of eggs and anti-apoptotis of cancer cells2008

    • Author(s)
      Ken-ichi Sato
    • Organizer
      International Symposium on Cell Signaling and Gene Regulation
    • Place of Presentation
      台湾・台南・台南大学
    • Year and Date
      2008-11-16
    • Related Report
      2009 Final Research Report
  • [Presentation] Roles of membrane microdomain-associated Src and uroplakin III in fertilization of eggs and anti-apoptosis of cancer cells2008

    • Author(s)
      Sato, K.
    • Organizer
      2008 International Symposium on Cell Signaling and Gene Regulation
    • Place of Presentation
      Tainan, TAIWAN
    • Year and Date
      2008-11-15
    • Related Report
      2008 Annual Research Report
  • [Presentation] チロシンリン酸化が制御するツメガエル卵の細胞機能:受精成立のシグナル伝達機構2008

    • Author(s)
      佐藤賢一
    • Organizer
      奈良先端科学技術大学院大学ワークショップ
    • Place of Presentation
      生駒・奈良先端科学技術大学院大学
    • Year and Date
      2008-09-29
    • Related Report
      2009 Final Research Report
  • [Presentation] チロシンリン酸化が制御するツメガエル卵の細胞機能 : 受精成立のシグナル伝達機構2008

    • Author(s)
      佐藤賢一
    • Organizer
      奈良先端科学技術大学院大学ワークショップ
    • Place of Presentation
      生駒
    • Year and Date
      2008-09-29
    • Related Report
      2008 Annual Research Report
  • [Presentation] 受精成立のシグナル伝達における細胞膜マイクロドメインの機能について2008

    • Author(s)
      佐藤賢一
    • Organizer
      日本動物学会第79回大会
    • Place of Presentation
      福岡
    • Year and Date
      2008-09-06
    • Related Report
      2008 Annual Research Report
  • [Presentation] Membrane microdomain-associated uroplakin III in eggs and cancer cells2008

    • Author(s)
      Sato, K.
    • Organizer
      FASEB Summer Research Conference 2008
    • Place of Presentation
      New Haven, USA
    • Year and Date
      2008-06-26
    • Related Report
      2008 Annual Research Report
  • [Presentation] Roles of Membrane Microdomains in Anti-apoptotic Growth of Human Bladder Carcinoma Cells2008

    • Author(s)
      Sato, K.
    • Organizer
      BIT' s Annual World Cancer Congress 2008
    • Place of Presentation
      Shanghai, CHINA
    • Year and Date
      2008-06-14
    • Related Report
      2008 Annual Research Report
  • [Presentation] Anti-apoptotic, survival mechanism of human bladder carcinoma cells in serum-free environment2008

    • Author(s)
      Sato, K.
    • Organizer
      Gordon Research Conference on Cell Death
    • Place of Presentation
      I1 Ciocco, ITALY
    • Related Report
      2008 Annual Research Report
  • [Presentation] Involvement of MT1-MMP, HB-EGF, and uroplakin III in serum-independent survival and growth of 5637 human bladder carcinoma cells2008

    • Author(s)
      Iwasaki, Tetsushi
    • Organizer
      Apoptosis 2008
    • Place of Presentation
      Luxembourg
    • Related Report
      2007 Annual Research Report
  • [Presentation] Focused Proteomics on Membrane Microdomain-associated Signal Transduction in Fertilized Amphibian Eggs and Human Bladder Carcinoma Cells2008

    • Author(s)
      Sato, Ken-ichi
    • Organizer
      International Conference of Drug Design and Discovery 2008
    • Place of Presentation
      Dubai, UAE
    • Related Report
      2007 Annual Research Report
  • [Presentation] Signal transduction at fertilization focusing on egg membrane microdomains: Partial proteolysis of uroplakin III and activation of the tyrosine kinase Src2007

    • Author(s)
      佐藤賢一
    • Organizer
      日本生化学会・分子生物学会合同大会
    • Place of Presentation
      横浜・パシフィコ横浜
    • Year and Date
      2007-12-12
    • Related Report
      2009 Final Research Report
  • [Presentation] Tyrosine phosphorylation signaling at oocytes maturation, fer-tilization, and early embryogenesis in Xenopus2007

    • Author(s)
      Sato, Ken-ichi
    • Organizer
      Gordon Research Conference on Fertilization & Activation of Development
    • Place of Presentation
      Holderness School, Plymouth, NH, USA
    • Year and Date
      2007-07-17
    • Related Report
      2007 Annual Research Report
  • [Presentation] 受精に伴う卵細胞膜マイクロドメイン局在性分子uroplakin IIIの部分分解とチロシンキナーゼSrc活性化の生理的意義2007

    • Author(s)
      佐藤賢一
    • Organizer
      第80回日本生化学会大会・第30回日本分子生物学会年会・合同大会(BMB2007)
    • Place of Presentation
      パシフィコ横浜
    • Related Report
      2007 Annual Research Report
  • [Presentation] 受精における配偶子相互作用と卵活性化シグナリングの分子機構2007

    • Author(s)
      佐藤賢一
    • Organizer
      日本ツメガエル研究集会
    • Place of Presentation
      札幌定山渓ビューホテル
    • Related Report
      2007 Annual Research Report
  • [Presentation] 卵黄タンパク質のチロシンリン酸化とその生理的・進化学的意義2007

    • Author(s)
      佐藤賢一
    • Organizer
      日本動物学会第78回大会
    • Place of Presentation
      弘前大学
    • Related Report
      2007 Annual Research Report
  • [Presentation] 受精シグナル伝達におけるPI 3-kinaseの関与2007

    • Author(s)
      ママドワギュナイ
    • Organizer
      日本動物学会第78回大会
    • Place of Presentation
      弘前大学
    • Related Report
      2007 Annual Research Report
  • [Presentation] Involvement of HB-EGF, MMP, and uroplakin III in serum-independent survival and growth of human bladder carcinoma cells2007

    • Author(s)
      佐藤賢一
    • Organizer
      第66回日本癌学会学術総会
    • Place of Presentation
      パシフィコ横浜
    • Related Report
      2007 Annual Research Report
  • [Presentation] 細胞膜マイクロドメインを介したシグナル伝達:アフリカツメガエル卵の受精成立とヒト膀胱がん細胞の血清飢餓抵抗性を例として2007

    • Author(s)
      深見泰夫
    • Organizer
      第80回日本生化学会大会・第30回日本分子生物学会年会・合同大会(BMB2007)
    • Place of Presentation
      パシフィコ横浜
    • Related Report
      2007 Annual Research Report
  • [Presentation] 活性化ゼノパス卵におけるhnRNP K結合mRNAの解析2007

    • Author(s)
      岩崎哲史
    • Organizer
      第80回日本生化学会大会・第30回日本分子生物学会年会・合同大会(BMB2007)
    • Place of Presentation
      パシフィコ横浜
    • Related Report
      2007 Annual Research Report
  • [Presentation] アフリカツメガエルにおける受精シグナル伝達および卵黄タンパク質チロシンリン酸化の解析2007

    • Author(s)
      Mammadova Gunay
    • Organizer
      第80回日本生化学会大会・第30回日本分子生物学会年会・合同大会(BMB2007)
    • Place of Presentation
      パシフィコ横浜
    • Related Report
      2007 Annual Research Report
  • [Book] 顕微鏡活用なるほどQ&A-意外に知らない基礎知識+一歩進んだ観察のコツもつかめる!2009

    • Author(s)
      佐藤賢一(分担執筆)宮戸健二, 岡部勝(編纂)
    • Publisher
      羊土社
    • Related Report
      2009 Final Research Report
  • [Book] 顕微鏡活用なるほどQ&A2008

    • Author(s)
      宮戸健二, 岡部勝(編さん)
    • Total Pages
      202
    • Publisher
      羊土社
    • Related Report
      2008 Annual Research Report
  • [Remarks]

    • Related Report
      2009 Final Research Report
  • [Remarks]

    • Related Report
      2009 Annual Research Report
  • [Remarks]

    • URL

      http://www.kyoto-su.ac.jp/department/bio/kyoin/sato/index.html

    • Related Report
      2008 Annual Research Report
  • [Remarks]

    • URL

      http://www.kyoto-su.ac.jp/department/bio/kyoin/sato/index.html

    • Related Report
      2007 Annual Research Report

URL: 

Published: 2007-04-01   Modified: 2016-04-21  

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