Primate pepsins : evolution, function, and adaptation to feeding strategy

• Project/Area Number: 19370102
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Anthropology
• Research Institution: Kyoto University
• Principal Investigator: KAGEYAMA Takashi Kyoto University, 霊長類研究所, 教授 (20027501)
• Co-Investigator(Kenkyū-buntansha): MIYABE Takako 京都大学, 霊長類研究所, 助教 (10437288) ; NARITA Yuichi 独立法人理化学研究所, 再生科学総合研究センター, 研究員 (40360614)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥17,225,000 (Direct Cost: ¥13,250,000、Indirect Cost: ¥3,975,000); Fiscal Year 2009: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2008: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2007: ¥8,125,000 (Direct Cost: ¥6,250,000、Indirect Cost: ¥1,875,000)
• Keywords: 霊長類 / 採食戦略 / ペプシン / 胃 / 消化酵素

Research Abstract

Pepsins are gastric digestive proteinases in vertebrates, including man. There are five forms, i.e., pepsins A, B, and F, gastricsin, and chymosin, which have evolved from a common ancestral aspartic proteinase. The specificity is well explained with the very hydrophobic nature of the S1' subsite, consisting of Tyr^<189>, Ile^<213>, Ile^<300>, Met^<289>, Val/Leu^<291>, and Leu^<298>. The first three residues are well conserved in pepsin family enzymes. Fourteen different pepsinogen-A cDNAs and one pepsinogen-C cDNA have been cloned from gastric mucosa of the orangutan, Pongo pygmaeus. The extreme multiplication in the orangutan might be advantageous for the digestion of herbaceous foods due to the increase in the level of enzymes in stomach and the diversification of enzyme specificity.

Research Products

• [Journal Article] Lineage-specific duplication and loss of pepsinogen genes in hominoid evolution. (2010)
  • Author(s): Narita, Y., Oda, S., Takenaka, O.,, Kageyama, T.
  • Journal Title: Journal of Molecular Evolution 70 Pages: 313-324
  • Peer Reviewed
• [Journal Article] Identification of essential residues of CTLA-2alpha for inhibitory potency. (2010)
  • Author(s): Deshapriya, R.M., Yuhashi, S., Usui, M., Kageyama, T.,, Yamamoto, Y.
  • Journal Title: Journal of Biochemistry 147 Pages: 393-404
  • Peer Reviewed
• [Journal Article] Differences in the P1' substrate specificities of pepsin A and chymosin. (2010)
  • Author(s): Kageyama, H., Ueda, H., Tezuka, T., Ogasawara, A., Narita, Y., Kageyama, T.,, Ichinose, M.
  • Journal Title: Journal of Biochemistry 147 Pages: 167-174
  • NAID: 10027873609
  • Peer Reviewed
• [Journal Article] Differences in the P1' substrate specificities of pepsin A and chymosin (2010)
  • Author(s): Kageyama, H
  • Journal Title: Journal of Biochemistry 147 Pages: 164-174
  • Peer Reviewed
• [Journal Article] Identification of essential residues of CTLA-2alpha for inhibitory potency (2010)
  • Author(s): Deshapriya, RM
  • Journal Title: Journal of Biochemistry 147 Pages: 393-404
  • Peer Reviewed
• [Journal Article] Structural and phylogenetic comparison of three pepsinogens from Pacific bluefin tuna: molecular evolution of fish pepsinogens. (2009)
  • Author(s): Tanji, M., Yakabe, E., Kubota, K., Kageyama, T., Ichinose, M., Miki, K., Ito, H.,, Takahashi, K.
  • Journal Title: Comparative Biochemistry and Physiology, part B, Biochemistry and Molecular Biology 152 Pages: 9-19
  • Peer Reviewed
• [Journal Article] Molecular cloning, gene expression, and tissue distribution of adiponectin and its receptors in the Japanese monkey, Macaca fuscata. (2009)
  • Author(s): Kang, K-H., Higashino, A., Kim, H-S., Lee, Y-T., Kageyama, T.
  • Journal Title: Journal of Medical Primatology 38 Pages: 77-85
  • Peer Reviewed
• [Journal Article] Development-dependent expression of calreticulin in the brain and other tissues of the Japanese monkey, Macaca fuscata. (2008)
  • Author(s): Higashino, A., Kageyama, T.
  • Journal Title: Journal of Medical Primatology 37 Pages: 303-310
  • Peer Reviewed
• [Journal Article] Redox-dependent structural ambivalence of the cytoplasmic domain in the inner ear-specific cadherin 23 isoform. (2008)
  • Author(s): Yonezawa, S., Hanai, A., Mutoh, N., Moriyama, A.,, Kageyama, T.
  • Journal Title: Biochemical and Biophysical Research Communications 366 Pages: 92-97
  • Peer Reviewed
• [Journal Article] Establishment of an ELISA system for the determination of Japanese monkey calreticulin and its application to plasma samples in macaques. (2008)
  • Author(s): Higashino, A., Yonezawa, S., Kageyama. T.
  • Journal Title: Journal of Medical Primatology 37 Pages: 340-346
  • Peer Reviewed
• [Journal Article] Development-dependent expression of calreticulin in the brain and other tissues of the Japanese monkey, Macaca (2008)
  • Author(s): Higashino, A
  • Journal Title: Journal of Medical Primatology 37 Pages: 303-310
  • Peer Reviewed
• [Journal Article] Structural and phylogenetic comparison of three pepsinogens from Pacific bluefin tuna : molecular (2008)
  • Author(s): Tanji, M
  • Journal Title: Comparative Biochemistry and Physiology, part B, Biochemistry and Molecular biology 152 Pages: 9-19
  • Peer Reviewed
• [Journal Article] Development-dependent expression of calreticulin in the brain and other tissues of the Tananese monkey, Macaca (2008)
  • Author(s): Higashino, A
  • Journal Title: Journal of Medical Primatology 37 Pages: 303-310
  • Peer Reviewed
• [Journal Article] Structural and phylogenetic comparison of three pepsinogens from Pacific bluefin tuna: molecular (2008)
  • Author(s): Tanji, M
  • Journal Title: Comparative Biochemistry and Physiology, part B, Biochemistry and Molecular biology 152 Pages: 9-19
  • Peer Reviewed
• [Journal Article] Polymerase chain reaction detection of Clostridium perfringens in feces from captive and wild chimpanzees, Pan troglodytes. (2007)
  • Author(s): Fujita, S., Kageyama, T.
  • Journal Title: Journal of Medical Primatology 36 Pages: 25-32
  • Peer Reviewed
• [Journal Article] Purification and characterization of pepsinogens from the gastric mucosa of African coelacanth, Latimeria chalumnae, and properties of the major pepsins. (2007)
  • Author(s): Tanji, M., Yakabe, E., Kageyama, T., Yokobori, S., Ichinose, M., Miki, K., Ito, H., Takahashi K.
  • Journal Title: Comparative Biochemistry and Physiology part B, Biochemistry and Molecular Biology 146 Pages: 412-420
  • Peer Reviewed