Novel crop improvement by RNA transported through phloem
Project/Area Number |
19380002
|
Research Category |
Grant-in-Aid for Scientific Research (B)
|
Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Breeding science
|
Research Institution | Hirosaki University |
Principal Investigator |
HARADA Takeo Hirosaki University, 農学生命科学部, 教授 (10228645)
|
Co-Investigator(Kenkyū-buntansha) |
SUGIYAMA Syuichi 弘前大学, 農学生命科学部, 教授 (00154500)
|
Project Period (FY) |
2007 – 2009
|
Project Status |
Completed (Fiscal Year 2009)
|
Budget Amount *help |
¥14,430,000 (Direct Cost: ¥11,100,000、Indirect Cost: ¥3,330,000)
Fiscal Year 2009: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2008: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2007: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
|
Keywords | 伴細胞プロモーター / Meトマト / PFP-T6 / 接ぎ木 / RNA / 篩管輸送 / リンゴ / 篩部 / 植物 / 矮化 / 育種学 / 園芸学 / 遺伝子 / 台木 / 穂木 |
Research Abstract |
To perform an effective RNA transport through phloem, the validity of the CoYMV promoter, reported as a companion specific function, was analyzed. We prepared the transgenic tobacco harboring a homeobox fusion gene PFP-T6. Then, wild type as scion was grafted onto the transgenic plants as stock. The grafts showed the transport of the PFP-T6 transcript through the graft union and the shape of scion leaves was changed. Furthermore, we developed the techniques in situ hybridization for phloem tissue cells and Agrobacterium transformation of Malus. These fruits were leveraged in the study of an another research project.
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Report
(4 results)
Research Products
(21 results)