| Budget Amount *help |
¥14,430,000 (Direct Cost: ¥11,100,000、Indirect Cost: ¥3,330,000)
Fiscal Year 2009: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2008: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2007: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
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| Research Abstract |
To perform an effective RNA transport through phloem, the validity of the CoYMV promoter, reported as a companion specific function, was analyzed. We prepared the transgenic tobacco harboring a homeobox fusion gene PFP-T6. Then, wild type as scion was grafted onto the transgenic plants as stock. The grafts showed the transport of the PFP-T6 transcript through the graft union and the shape of scion leaves was changed. Furthermore, we developed the techniques in situ hybridization for phloem tissue cells and Agrobacterium transformation of Malus. These fruits were leveraged in the study of an another research project.
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