| Project/Area Number |
19380004
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Breeding science
|
|---|
| Research Institution | The University of Tokyo |
|---|
Principal Investigator |
NAKAZONO Mikio The University of Tokyo, 大学院・農学生命科学研究科, 准教授 (70282697)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
堤 伸浩 東京大学, 大学院・農学生命科学研究科, 教授 (00202185)
木下 哲 奈良先端科学技術大学院, 大学バイオサイエンス研究科, 准教授 (60342630)
石丸 努 独立行政法人農業食品産業技術総合研究機構, 作物研究所, 任期付研究員 (40414635)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
TSUTSUMI Nobuhiro 東京大学, 大学院・農学生命科学研究科, 教授 (00202185)
KINOSHITA Tetsu 奈良先端科学技術大学, 大学院・バイオサイエンス研究科, 准教授 (60342630)
ISHIMARU Tsutomu 独立行政法人農業食品産業技術総合研究機構, 作物研究所, 研究員 (40414635)
|
|---|
| Project Period (FY) |
2007 – 2009
|
| Project Status |
Completed (Fiscal Year 2009)
|
| Budget Amount *help |
¥20,410,000 (Direct Cost: ¥15,700,000、Indirect Cost: ¥4,710,000)
Fiscal Year 2009: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2008: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
Fiscal Year 2007: ¥9,620,000 (Direct Cost: ¥7,400,000、Indirect Cost: ¥2,220,000)
|
|---|
| Keywords | レーザーマイクロダイセクション / DNAメチレーション / イネ / 生殖系列 / DNAメチル化 / タイリングアレイ / LMD |
|---|
| Research Abstract |
In this research, we tried to investigate DNA methylation pattern in laser microdissection (LMD)-isolated embryo and endosperm by a tiling array. We examined optimal conditions for bisulfite sequencing using total DNA extracted from some tissues in rice. We succeeded in the bisulfite sequencing when we used enough amounts of DNA, but failed in the sequencing using small amounts of DNA extracted from the LMD isolated tissues. As a result, we did not succeed in solution of this problem, and thus were not able to carry out the tiling array. We are continuing to examine the optimal conditions for the bisulfite sequencing using small amounts of DNA.
|
