Elucidation of the molecular genetic mechanism involved in theself/non-self recognition of self-incompatibility in a plant family, Convolvulaceae

• Project/Area Number: 19380005
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Breeding science
• Research Institution: Mie University
• Principal Investigator: KOWYAMA Yasuo Mie University, 大学院・生物資源学研究科, 特任教授 (80024579)
• Co-Investigator(Kenkyū-buntansha): KAKEDA Katsuyuki 三重大学, 大学院・生物資源学研究科, 准教授 (50221867) ; TSUCHIYA Tohru 三重大学, 生命科学研究支援センター, 准教授 (30293806)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥12,870,000 (Direct Cost: ¥9,900,000、Indirect Cost: ¥2,970,000); Fiscal Year 2008: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000); Fiscal Year 2007: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
• Keywords: 植物生殖様式 / 自家不和合性 / ヒルガオ科 / pomoea属 / サツマイモ野生種 / 分子遺伝学 / ゲノム解析 / 形質転換植物 / Ipomoea属 / 生殖様式 / 遺伝子組換え植物

Research Abstract

種々の植物が有する自家不和合性は、自家受精を抑制し後代子孫の内婚弱勢を回避するのに寄与している遺伝的な性質である。本研究では、ヒルガオ科の自家不和合性に関与する遺伝子を明らかにしその分子機構を解明することを目的として、サツマイモ野生種のゲノム解析と遺伝子発現解析を行った。その結果、ヒルガオ科の自家不和合性に関与する雌蕊側と花粉側の各S遺伝子候補が同定され、新たな自他認識機構の関与が示唆された。

Research Products

• [Journal Article] Deletion mutations of the self-incompatibility (S) locus induced by gamma irradiation in a wild diploid species of sweet potato (2009)
  • Author(s): Kakeda K., Tsutsumi M. and Kowyama Y.
  • Journal Title: Ipomoea trifida. JARQ (in press)
  • Peer Reviewed
• [Journal Article] Agrobacterium-mediated transformation of Ipomoea trifida, a diploid relative of sweetpotato (2009)
  • Author(s): Kakeda K., Urabayashi T., Ohashi T., Oguro T.and Kowyama Y.
  • Journal Title: Breed. Sci 59 Pages: 95-98
  • Peer Reviewed
• [Journal Article] Agrobacterium-mediated transformation of Ipomoea trifida, a diploid relative of sweet potato. (2009)
  • Author(s): Kakeda K, Urabayashi T, Ohashi T, Oguro T, Kowyai Y.
  • Journal Title: Breed. Sci. 59(印刷中)
  • Peer Reviewed
• [Journal Article] Molecular and genetic characterization of the Slocus in Hordeum bulbosum L., a wildself-incompatible species related to cultivatedbarley. Mol (2008)
  • Author(s): Kakeda K., Ibuki T., Suzuki J., Tadano H.,Kurita Y., Hanai Y. and Kowyama Y.
  • Journal Title: Genet. Genomics 280 Pages: 509-519
  • Peer Reviewed
• [Journal Article] Nomura M., Suzuki T.,Matsumoto T., Sato K., Kanamori H., KawasakiS., Takeda K. Barley grain with adhering hullsis controlled by an ERF family transcriptionfactor gene regulating a lipid biosynthesispathway (2008)
  • Author(s): Taketa S., Amano S., Tsujino Y., Sato T., SaishoD., Kakeda K.
  • Journal Title: Proc. Natl. Acad. Sci. USA 105 Pages: 4062-4067
  • Peer Reviewed
• [Journal Article] Barley grain with adhering hulls is controlled by an ERF family transcription factor gene regulating alipid biosynthesis pathway (2008)
  • Author(s): Taketa S, Amano S, Tsujino Y, Sato T, Saisho D, Kakeda K, Nomura M, Suzuki T, Matsumoto T, Sato K, Kanamori H, Kawasaki S, Takeda K.
  • Journal Title: Proc. Natl. Acad. Sci. U.S.A. 105 Pages: 4062-4067
  • Peer Reviewed
• [Journal Article] Molecular and genetic characterization of the S locus in Hordeum bulbosum L, a wild self-incompatible species related to cultivated barley (2008)
  • Author(s): Kakeda K, Ibuki T, Suzuki J, Tadano H, Kurita Y. Hanai Kowyama Y.
  • Journal Title: Mol. Genet. Genomics 280 Pages: 509-519
  • Peer Reviewed
• [Journal Article] Barley grain with adhering hulls is controlled by an ERF family transcription factor gene regulating a lipid biosynthesis pathway (2008)
  • Author(s): Taketa S, Amano S, Tsujino Y, Sato T, Saisho D, Kakeda K, Nomura M, Suzuki T, Matsumoto T, Sato K, Kanamori H, Kawasaki S, Takeda K.
  • Journal Title: Proc.Natl.Acad.Sci.U.S.A. 105 Pages: 4062-4067
  • Peer Reviewed
• [Journal Article] Physical size of the S locusregion defined by genetic recombination and genome sequencing in Ipomoea trifida, Convolvu (2007)
  • Author(s): Rahman H., Tsuchiya T., Suwabe K., Kohori J., Tomita R., Kagaya Y., Kobayashi I., Kakeda K.,and Kowyama Y.
  • Journal Title: laceae Sexual Plant Reproduction 20 Pages: 63-72
  • Peer Reviewed
• [Journal Article] Expression of stigma-and anther-specific geneslocated in the Slocus region of Ipomoea trifida (2007)
  • Author(s): Rahman H., Uchiyama M., Kuno M., HirashimaN., Suwabe K., Tsuchiya T., Kagaya Y.,Kobayashi I., Kakeda K., and Kowyama Y
  • Journal Title: Sexual Plant Reproduction 20 Pages: 73-85
  • Peer Reviewed
• [Journal Article] Physical size of the S locus region defined by genetic recombination and genome sequencing in Ipomoea trcfida, Convolvulaceae. (2007)
  • Author(s): Rahman MH, Tsuchiya T, Suwabe K Kohori J, Tomita RN, Kagaya Y, Ko-byashi I, Kakeda K, Kowama Y.
  • Journal Title: Sexual Plant Reproduction 20 Pages: 63-72
  • Peer Reviewed
• [Journal Article] Expression of stigma-and anther-specific genes located in the S locus region of Ipomoea trifida. (2007)
  • Author(s): Rahman MH, Uchiyama M, Kuno M, Hirashima N, Suwabe K, Tsuchi-ya T, Kagaya Y, Kobayashi I, Kake-da K, Kowyama Y.
  • Journal Title: Sexual Plant Reproduction 20 Pages: 73-85
  • Peer Reviewed
• [Presentation] オオムギ野生種におけるSハプロタイプ間の比較ゲノム解析 (2009)
  • Author(s): 掛田克行・大岸康佑・石濱裕子
  • Organizer: 日本育種学会
  • Place of Presentation: つくば国際会議場
  • Year and Date: 2009-03-28
• [Presentation] オオムギ野生種におけるSタハプロタイプ間の比較ゲノム解析 (2009)
  • Author(s): 掛田克行, 大岸康佑, 石濱裕子
  • Organizer: 日本育種学会
  • Place of Presentation: つくば国際会議場
  • Year and Date: 2009-03-28
• [Presentation] 遺伝子導入法を用いたオオムギ皮裸性関連遺伝子の解析 (2008)
  • Author(s): 清水匡史・堀口修吾・石原倫光、掛田克行
  • Organizer: 日本育種学会中部地区談話会
  • Place of Presentation: 名城大学農学部
  • Year and Date: 2008-11-29
• [Presentation] オオムギ野生種にけるS候補遺伝子周辺ゲノム領域の塩基配列解析 (2008)
  • Author(s): 大岸康佑・掛田克行
  • Organizer: 日本育種学会中部地区談話会
  • Place of Presentation: 名城大学農学部
  • Year and Date: 2008-11-29
• [Presentation] オオムギ野生種におけるS候補遺伝子周辺ゲノム領域の塩基配列解析 (2008)
  • Author(s): 大岸康佑, 掛田克行
  • Organizer: 日本育種学会巾部地区談話会
  • Place of Presentation: 名城大学
  • Year and Date: 2008-11-29
• [Presentation] 遺伝子導入法を用いたオオムギ皮裸性関運遺伝子の解析 (2008)
  • Author(s): 清水匡史, 堀口修吾, 石原倫光, 掛田克行
  • Organizer: 日本育種学会中部地区談話会
  • Place of Presentation: 名城大学
  • Year and Date: 2008-11-29
• [Presentation] オオムギ皮裸性遺伝子(Nud)を導入した形質転換イネの解析 (2008)
  • Author(s): 石原倫光・清水匡史・掛田克行・武田真
  • Organizer: 日本育種学会
  • Place of Presentation: 滋賀県立大学
  • Year and Date: 2008-10-11
• [Presentation] オオムギ野生種のS遺伝子座周辺ゲノム領域の分子的解析 (2008)
  • Author(s): 掛田克行・岸康佑
  • Organizer: 日本育種学会
  • Place of Presentation: 滋賀県立大学
  • Year and Date: 2008-10-11
• [Presentation] Genomic organization of the sporophyticself-incompatibility locus in Ipomoea trifida, aclose relative of sweet potato (2008)
  • Author(s): Kowyama Y., Tsuchiya T. and Kakeda K
  • Organizer: 20th InternationalCongress on Sexual Plant Reproduction
  • Place of Presentation: Brasilia, Brazil
  • Year and Date: 2008-08-05
• [Presentation] Genomic organization of the sporophytic self incompatibility locus in Ipomoea trifida, a close relative of sweet potato. (2008)
  • Author(s): Kowyama. Y., Tsuchiva, T., Kakeda, K.
  • Organizer: 20th International Congress on Sexual Plant Reproduction
  • Place of Presentation: Kubitschek Plaza Hotel, Brasilia, Brazil
  • Year and Date: 2008-08-05
• [Presentation] オオムギ野生種のSハプロタイプ特異的タンパク質の解析 (2008)
  • Author(s): 掛田克行・栢部健人
  • Organizer: 日本育種学会
  • Place of Presentation: 明治大学農学部
  • Year and Date: 2008-03-29
• [Presentation] オオムギ野生種におけるS候補遺伝子およびその周辺ゲノム領域の解析 (2007)
  • Author(s): 大岸康佑・栢部健人・掛田克行
  • Organizer: 日本育種学会中部地区談話会
  • Place of Presentation: 信州大学農学部
  • Year and Date: 2007-12-01
• [Presentation] サツマイモ野生種のSハプロタイプ間におけるゲノム配列多型性と優劣性関係 (2007)
  • Author(s): 神山康夫・M.H.Rahman・高北英治・谷口理・掛田克行・土屋亨
  • Organizer: 日本育種学会
  • Place of Presentation: 山形大学農学部
  • Year and Date: 2007-09-23
• [Book] Diversity, and Mechanisms. (ed.Franklin-Tong, V.E.) (2008)
  • Author(s): Kowyama, Y., Tsuchiya, T, and Kakeda, K
  • Publisher: Springer-Verlag, Berlin Heidelberg, Moleculargenetics of sporophytic self-incompatibility inIpomoea, a member of the Convolvulaceae. In : Self-Incompatibility in Flowering Plants-Evolution
• [Book] In : Handbook of New technologies for geneticimprovement of legumes. (ed. Kirti P. B.) (2008)
  • Author(s): Watanabe, M., Endo, M., Hakozaki, H., Masuko,H., Park, J-I., Kawagishi-Kobayashi, M.,Tsuchiya, T
  • Publisher: CRC Press, Gene expressionanalysis with DNA array technology in Legumes
• [Book] Molecular genetics of sporophytic self-incompatibility in Ipomoea, a member of the Convolvulaceae. In "Self-Incompatibilitv in Flowering Plants- Evolution, Diversity, and Mechanisms" (ed. V. E. Franklin-Tong) (2008)
  • Author(s): Kowvama Y, Tsuchiva T, Kakeda K
  • Publisher: Springer-Verlag, Berlin Heidelberg
• [Book] Gene expression analysis with DNA array technology in Legumes. In "Handbook of new technologies for genetic improvement of legumes" (ed. Kirti P. B. ) (2008)
  • Author(s): Watanabe M., Endo M., Hakozaki H., Masuko H., Park J., Tsuchiva T.
  • Publisher: CRC Press
• [Book] Molecular genetics of sporophytic self-incompatibility in Ipomoea, a member of the Convolwlaceae (2008)
  • Author(s): Kowyama Y, Tsuchiya T, Kakeda K
  • Publisher: Springer-Verlag(印刷中)
• [Remarks]
  • URL: http://miuse.mie-u.ac.jp:8080/items-by-author?autor=Kowyama+Yasuo
• [Remarks]
  • URL: http://miuse.mie-u.ac.jp:8080/items-by-author?author=Kowyama,+Yasuo
• [Remarks]
  • URL: http://miuse.mie-u.ac.jp:8080/items-by-author?author=Kowyama,+Yasuo