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Production of tailor-made starches in starch synthase-related double mutants and transgenic rice.

Research Project

Project/Area Number 19380007
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Breeding science
Research InstitutionAkita Prefectural University

Principal Investigator

FUJITA Naoko  Akita Prefectural University, 生物資源科学部, 准教授 (90315599)

Co-Investigator(Kenkyū-buntansha) ITOH Kimiko  新潟大学, 自然科学系, 准教授 (10281007)
AKUZAWA Sayuri  東京農業大学, 応用生物科学部, 准教授 (60256641)
HANASHIRO Isao  鹿児島大学, 農学部, 准教授 (30336325)
Project Period (FY) 2007 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥20,280,000 (Direct Cost: ¥15,600,000、Indirect Cost: ¥4,680,000)
Fiscal Year 2009: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2008: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2007: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
Keywordsデンプン科学 / 変異体 / 組換体 / イネ / デンプン / アミロペクチン / スターチシンターゼ / アミロース
Research Abstract

At least four classes of enzymes responsible for starch biosynthesis in higher plants have been known. Rice mutants deficient in specific isozymes of each class accumulate unique starch in the endosperm. Starch synthase (SS), which elongate linear glucosyl-chains of starch, have the largest numbers of isozymes, and each SS isozyme has been suggested to play distinct roles. In this study, double mutants of SS and other classes and transgenic lines were produced. Their starches show unique physicochemical properties that are useful for production of food, foodstuffs, and other materials in industrial scale.

Report

(4 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • 2007 Annual Research Report
  • Research Products

    (65 results)

All 2010 2009 2008 2007 Other

All Journal Article (23 results) (of which Peer Reviewed: 21 results) Presentation (38 results) Patent(Industrial Property Rights) (4 results)

  • [Journal Article] Biosynthesis and Degradation of Starch. Bull. Facul. Agric2010

    • Author(s)
      Mitsui T, Itoh K, Hori H, Hiroyuki Ito H
    • Journal Title

      Niigata Univ. 62(2)

      Pages: 49-73

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Biosynthesis and Degradation of Starch.2010

    • Author(s)
      Mitsui, et al.(伊藤紀美子)
    • Journal Title

      Bull.Facul.Agric, Niigata Univ. 62

      Pages: 49-73

    • NAID

      40017169788

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of PUL-deficient mutants of rice (Oryza sativa L. ) and the function of PUL on the starch biosynthesis in the rice endosperm.2009

    • Author(s)
      Fujita N(1番目), …, Hanashiro I(5番目), …, Akuzawa S(7番目)et, al., (15名)
    • Journal Title

      Journal of Experimental Botany 60

      Pages: 1009-1023

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Screening of Pullulanase deficient mutants of rice.2009

    • Author(s)
      Toyosawa Y, Fujita N(2番目)et, al., (5名)
    • Journal Title

      Rice Genetic Newsletters 25

      Pages: 42-43

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Revealing the complex system of starch biosynthesis in higher plants using rice mutants and transformations.2009

    • Author(s)
      Nakamura Y, Fujita N, Utsumi Y, Nishi A, Satoh, H.Q.Y. Shu (ed. )
    • Journal Title

      Food and Agriculture Organization if the United Nations, Rome

      Pages: 183-186

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Structures of endosperm starch from a rice wx cultiar expression Wxa transgene.2009

    • Author(s)
      Hanashiro I(1番目), …, Itoh K(5番目)et, al., (7名)
    • Journal Title

      Journal of Applied Glycoscience 56

      Pages: 65-70

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Screening of Pullulanase deficient mutants of rice.2009

    • Author(s)
      Toyosawa, et al.(藤田直子)
    • Journal Title

      Rice Genetic Newsletters 25(Web公開)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Revealing the complex system of starch biosynthesis in higher plants using rice mutants and transformations.2009

    • Author(s)
      Nakamura, et al.(藤田直子)
    • Journal Title

      Food and Agriculture Organization if the United Nations, Rome

      Pages: 183-186

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Structures of endosperm starch from a rice wx cultiar expression Wx^a transgene.2009

    • Author(s)
      Hanashiro, et al.(花城勲, 伊藤紀美子)
    • Journal Title

      J.Appi.Glycosci. 56

      Pages: 65-70

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of PUL-deficient mutants of rice(Oryza sativa L. )and the function of PUL on the starch biosynthesis in the rice endosperm.2009

    • Author(s)
      Fujita, et al. (藤田直子, 花城勲, 阿久澤さゆり)
    • Journal Title

      Journal of Experimental Botany 60

      Pages: 1009-1023

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 2007年Starch Round Table (SRT) in San Antonioに参加して.2008

    • Author(s)
      藤田直子
    • Journal Title

      Journal of Applied Glycoscience 55

      Pages: 68-69

    • NAID

      10020061122

    • Related Report
      2009 Final Research Report
  • [Journal Article] The function of rice starch synthase I expressed in E. coli.2008

    • Author(s)
      Fujita N, Goto S, Yoshida M, Suzuki E, Nakamura Y
    • Journal Title

      Journal of Applied Glycoscience 55

      Pages: 167-172

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Granule-bound starch synthase I is responsible for biosynthesis of extra-long unit chains of amylopectin in rice.2008

    • Author(s)
      Hanashiro I(1番目), Itoh K(2番目)et, al. (7名)
    • Journal Title

      Plant and Cell Physiology 49

      Pages: 925-933

    • NAID

      10027331844

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Plastidicα-glucan phosphorylase mutation dramatically affects the synthesis and structure of starch in rice endosperm.2008

    • Author(s)
      Satoh H, …, Fujita N(9番目)et, al., (15名)
    • Journal Title

      The Plant Cell 20

      Pages: 1833-1849

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Establishment o high-amylose type Japonica rice by Agrobacterium-mediated gene transfer.2008

    • Author(s)
      Hasegawa M, Sekikawa A, Tanno F, Itoh K.
    • Journal Title

      Bull. Facul. Agric. Niigata Univ. 61(1)

      Pages: 41-45

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] The function of rice starch synthase I expressed in E. coli.2008

    • Author(s)
      Fujita, et al. (藤田直子)
    • Journal Title

      Journal of Applied Glycoscience 55

      Pages: 167-172

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Granule-bound starch synthase I is responsible for biosynthesis of extra-long unit-chains of amylopectin in rice.2008

    • Author(s)
      Hanashiro, et al. (花城勲, 伊藤紀美子)
    • Journal Title

      Plant and Cell physiology 49

      Pages: 925-933

    • NAID

      10027331844

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Plastidic α-glucan phosphorylase mutation dramatically affects the synthesis and structure of starch in rice endosperm.2008

    • Author(s)
      Satoh, et al. (藤田直子)
    • Journal Title

      The Plant Cell 20

      Pages: 1833-1849

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 2007年Starch Round Table (SRT) in San Antonioに参加して。2008

    • Author(s)
      藤田直子
    • Journal Title

      Journal of Applied Glycoscience 55

      Pages: 68-69

    • NAID

      10020061122

    • Related Report
      2007 Annual Research Report
  • [Journal Article] Characterization of SSIIIa-deficient mutants of rice (Oryza sativa L. ); the fucntion of SSIIIa and pleiotropic effects by SSIIIa deficiency in the rice endosperm.2007

    • Author(s)
      Fujita N(1番目)et, al. (13名)
    • Journal Title

      Plant Physiology 144

      Pages: 2009-2023

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of SSIIIa-deficient mutants of rice (Oryza sativa L.); the fucntion of SSIIIa and pleiotropic effects by SSIIIa deficiency in the rice endosperm.2007

    • Author(s)
      Fujita, et. al.(藤田直子ら)
    • Journal Title

      Plant Physiology 144

      Pages: 2009-2023

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The function of rice starch synthase I expressed in E. coli.

    • Author(s)
      Fujita, et. al.(藤田直子ら)
    • Journal Title

      Journal of Applied Glycoscience (掲載確定)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Granule-bound starch synthase I is responsible for biosynthesis of extra-long unit-chains of amylopectin in rice

    • Author(s)
      Hanashiro, et. al.(花城勲ら)
    • Journal Title

      Plant and Cell Physiology (掲載確定)

    • NAID

      10027331844

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] スターチシンターゼアイソザイムによってフィトグリコーゲンの構造を改変させたイネの作出2010

    • Author(s)
      藤田直子, ら(藤田直子)
    • Organizer
      日本農芸化学会
    • Place of Presentation
      東京大学
    • Year and Date
      2010-03-29
    • Related Report
      2009 Final Research Report
  • [Presentation] スターチシンターゼアイソザイムによってフィトグリコーゲンの構造を改変させたイネの作出2010

    • Author(s)
      藤田直子, ら(藤田直子)
    • Organizer
      日本農芸化学会平成22年度大会
    • Place of Presentation
      東京大学
    • Year and Date
      2010-03-29
    • Related Report
      2009 Annual Research Report
  • [Presentation] アミロライスの開発とその特性の研究(2)2010

    • Author(s)
      阿部克, ら(伊藤紀美子, 藤田直子)
    • Organizer
      日本育種学会
    • Place of Presentation
      京都大学
    • Year and Date
      2010-03-26
    • Related Report
      2009 Final Research Report
  • [Presentation] アミロライスの開発とその特性の研究(2)2010

    • Author(s)
      阿部克, ら(伊藤紀美子, 藤田直子)
    • Organizer
      日本育種学会2010年度春季大会
    • Place of Presentation
      京都大学
    • Year and Date
      2010-03-26
    • Related Report
      2009 Annual Research Report
  • [Presentation] スターチシンターゼSSI/SSIIIa活性が低下したイネ変異体2010

    • Author(s)
      藤田直子, ら(藤田直子)
    • Organizer
      日本植物生理学会
    • Place of Presentation
      熊本大学
    • Year and Date
      2010-03-17
    • Related Report
      2009 Final Research Report
  • [Presentation] スターチシンターゼSSI/SSIIIa活性が低下したイネ変異体2010

    • Author(s)
      藤田直子, ら(藤田直子)
    • Organizer
      第51回日本植物生理学会
    • Place of Presentation
      熊本大学
    • Year and Date
      2010-03-17
    • Related Report
      2009 Annual Research Report
  • [Presentation] ユニークな澱粉を貯める二重変異体の開発2009

    • Author(s)
      藤田直子
    • Organizer
      アグリビジネス創出フェア2009
    • Place of Presentation
      幕張市
    • Year and Date
      2009-11-25
    • Related Report
      2009 Annual Research Report 2009 Final Research Report
  • [Presentation] イネsugary-1変異体が蓄積するフィトグリコーゲンの構造を改変させたイネの作出2009

    • Author(s)
      藤田直子, ら(藤田直子)
    • Organizer
      日本育種学会
    • Place of Presentation
      北海道大学
    • Year and Date
      2009-09-25
    • Related Report
      2009 Final Research Report
  • [Presentation] イネsugary-1変異体が蓄積するフィトグリコーゲンの構造を改変させたイネの作出2009

    • Author(s)
      藤田直子, ら(藤田直子)
    • Organizer
      日本育種学会平成21年度大会
    • Place of Presentation
      北海道大学
    • Year and Date
      2009-09-25
    • Related Report
      2009 Annual Research Report
  • [Presentation] 外部鎖長が変化したフィトグリコーゲンを蓄積するイネの作出2009

    • Author(s)
      藤田直子, ら(藤田直子)
    • Organizer
      日本応用糖質科学会
    • Place of Presentation
      弘前大学
    • Year and Date
      2009-09-16
    • Related Report
      2009 Final Research Report
  • [Presentation] 澱粉合成酵素SSI、SSIIIa活性の欠損によるイネ胚乳澱粉の分子構造変化2009

    • Author(s)
      樋口利幸, ら(花城勲, 藤田直子)
    • Organizer
      日本応用糖質科学会
    • Place of Presentation
      弘前大学
    • Year and Date
      2009-09-16
    • Related Report
      2009 Final Research Report
  • [Presentation] 外部鎖長が変化したフィトグリコーゲンを蓄積するイネの作出2009

    • Author(s)
      藤田直子, ら(藤田直子)
    • Organizer
      日本応用糖質科学会平成21年度大会
    • Place of Presentation
      弘前大学
    • Year and Date
      2009-09-16
    • Related Report
      2009 Annual Research Report
  • [Presentation] 澱粉合成酵素SSI、SSIIIa活性の欠損によるイネ胚乳澱粉の分子構造変化2009

    • Author(s)
      樋口利幸, ら(花城勲, 藤田直子)
    • Organizer
      日本応用糖質科学会平成21年度大会
    • Place of Presentation
      弘前大学
    • Year and Date
      2009-09-16
    • Related Report
      2009 Annual Research Report
  • [Presentation] The novel starches accumulated in the rice mutants having extremely low starch synthase I (SS)/SSIIIa.2009

    • Author(s)
      Fujita N
    • Organizer
      Starch Round Table 2009
    • Place of Presentation
      ボルチモア(USA)
    • Year and Date
      2009-09-12
    • Related Report
      2009 Final Research Report
  • [Presentation] イネスターチシンターゼ(SSIIIa)変異体米澱粉の分子構造と利用性に関する研究2009

    • Author(s)
      池上享, ら(阿久澤さゆり, 藤田直子)
    • Organizer
      日本食品科学工学会
    • Place of Presentation
      名城大学
    • Year and Date
      2009-09-12
    • Related Report
      2009 Annual Research Report 2009 Final Research Report
  • [Presentation] The novel starches accumulated in the rice mutants having extremely low starch synthase I (SS)/SSIIIa.2009

    • Author(s)
      N.Fujita(藤田直子)
    • Organizer
      Starch Round Table 2009
    • Place of Presentation
      ボルチモア(アメリカ合衆国)
    • Year and Date
      2009-09-12
    • Related Report
      2009 Annual Research Report
  • [Presentation] 高アミロース米の研究2009

    • Author(s)
      阿部克, ら(藤田直子、伊藤紀美子)
    • Organizer
      日本農芸化学会
    • Place of Presentation
      福岡市マリンメッセ
    • Year and Date
      2009-03-29
    • Related Report
      2009 Final Research Report 2008 Annual Research Report
  • [Presentation] SSI/SSIIIa活性が低下したイネ変異体のユニークな澱粉構造2009

    • Author(s)
      藤田直子, ら(藤田直子)
    • Organizer
      日本農芸化学会
    • Place of Presentation
      福岡市マリンメッセ
    • Year and Date
      2009-03-29
    • Related Report
      2009 Final Research Report
  • [Presentation] SSI/SSIIIa活性が低下しかイネ変異体のユニークな澱粉構造2009

    • Author(s)
      藤田直子ら(藤田直子)
    • Organizer
      日本農芸化学会
    • Place of Presentation
      福岡市マリンメッセ
    • Year and Date
      2009-03-29
    • Related Report
      2008 Annual Research Report
  • [Presentation] アミロライスの開発とその特性の研究2008

    • Author(s)
      阿部克, ら(藤田直子、伊藤紀美子)
    • Organizer
      日本育種学会
    • Place of Presentation
      滋賀県立大学
    • Year and Date
      2008-10-11
    • Related Report
      2009 Final Research Report
  • [Presentation] 高アミロースデンプンを蓄積するイネ変異体系統の解析2008

    • Author(s)
      藤田直子, ら(藤田直子)
    • Organizer
      日本育種学会
    • Place of Presentation
      滋賀県立大学
    • Year and Date
      2008-10-11
    • Related Report
      2009 Final Research Report
  • [Presentation] 高アミロースデンプンを蓄積するイネ変異体系統の解析2008

    • Author(s)
      藤田直子ら(藤田直子)
    • Organizer
      日本育種学会
    • Place of Presentation
      彦根市滋賀県立大学
    • Year and Date
      2008-10-11
    • Related Report
      2008 Annual Research Report
  • [Presentation] アミロライスの開発とその特性の研究2008

    • Author(s)
      阿部克ら(藤田直子, 伊藤紀美子)
    • Organizer
      日本育種学会
    • Place of Presentation
      彦根市滋賀県立大学
    • Year and Date
      2008-10-11
    • Related Report
      2008 Annual Research Report
  • [Presentation] コメデンプンの改変2008

    • Author(s)
      藤田直子
    • Organizer
      東京農業大学先端研究シンポジウム(招待講演)
    • Place of Presentation
      東京農業大学世田谷キャンパス
    • Year and Date
      2008-10-03
    • Related Report
      2009 Final Research Report 2008 Annual Research Report
  • [Presentation] イネ枝作り酵素( BE)の二重変異の胚乳澱粉特性2008

    • Author(s)
      西愛子, ら(藤田直子)
    • Organizer
      日本応用糖質科学会
    • Place of Presentation
      琉球大学
    • Year and Date
      2008-09-18
    • Related Report
      2009 Final Research Report
  • [Presentation] スターチシンターゼSSI/SSIIIa活性が低下したイネ変異体の解析2008

    • Author(s)
      藤田直子, ら(藤田直子)
    • Organizer
      日本応用糖質科学会
    • Place of Presentation
      琉球大学
    • Year and Date
      2008-09-18
    • Related Report
      2009 Final Research Report
  • [Presentation] スターチシンターゼSSI/SSIIIa活性が低下しかイネ変異体の解析2008

    • Author(s)
      藤田直子ら(藤田直子)
    • Organizer
      日本応用糖質科学会
    • Place of Presentation
      那覇市琉球大学
    • Year and Date
      2008-09-18
    • Related Report
      2008 Annual Research Report
  • [Presentation] イネ枝作り酵素(BE)の二重変異の胚乳澱粉特性2008

    • Author(s)
      西愛子ら(藤田直子)
    • Organizer
      日本応用糖質科学会
    • Place of Presentation
      那覇市琉球大学
    • Year and Date
      2008-09-18
    • Related Report
      2008 Annual Research Report
  • [Presentation] GBSSIタンパク質の生化学的機能解析2007

    • Author(s)
      山崎美奈, ら(伊藤紀美子)
    • Organizer
      日本分子生物学会日本生化学会
    • Place of Presentation
      神戸ポートピアアイランド
    • Year and Date
      2007-12-12
    • Related Report
      2009 Final Research Report
  • [Presentation] GBSSIタンパク質の生化学的機能解析2007

    • Author(s)
      山崎美奈
    • Organizer
      第30回日本分子生物学会年会、第80回日本生化学会大会
    • Place of Presentation
      神戸ポートピアアイランド
    • Year and Date
      2007-12-12
    • Related Report
      2007 Annual Research Report
  • [Presentation] Distinct functions of mu ltiple starch synthase isozymes in r ice.2007

    • Author(s)
      藤田直子
    • Organizer
      Starch Round Table 2007
    • Place of Presentation
      サンアントニオ(USA)
    • Year and Date
      2007-10-05
    • Related Report
      2009 Final Research Report
  • [Presentation] Distinct functions of multiple starch synthase isozymes in rice.2007

    • Author(s)
      藤田直子
    • Organizer
      Starch Round Table 2007
    • Place of Presentation
      アメリカ合衆国,テキサス州サンアントニオ
    • Year and Date
      2007-10-05
    • Related Report
      2007 Annual Research Report
  • [Presentation] イネスターチシンターゼ(SSI)のインビトロ機能解析2007

    • Author(s)
      藤田直子
    • Organizer
      日本育種学会第112回講演会
    • Place of Presentation
      山形大学鶴岡キャンパス
    • Year and Date
      2007-09-23
    • Related Report
      2007 Annual Research Report
  • [Presentation] イネスターチシンターゼI(SSI)の機能解析2007

    • Author(s)
      藤田直子, ら(藤田直子)
    • Organizer
      日本応用糖質科学会
    • Place of Presentation
      日本大学藤沢キャンパス
    • Year and Date
      2007-08-29
    • Related Report
      2009 Final Research Report
  • [Presentation] イネスターチシンターゼI(SSI)の機能解析2007

    • Author(s)
      藤田直子
    • Organizer
      日本応用糖質科学会平成19年度大会
    • Place of Presentation
      日本大学藤沢キャンパス
    • Year and Date
      2007-08-29
    • Related Report
      2007 Annual Research Report
  • [Presentation] 胚乳特異的RNAi法による高アミロース成分米の研究2007

    • Author(s)
      関川晶子, ら(伊藤紀美子)
    • Organizer
      日本農芸化学会
    • Place of Presentation
      東京農業大学世田谷キャンパス
    • Year and Date
      2007-03-25
    • Related Report
      2009 Final Research Report
  • [Presentation] イネスターチシンターゼSSIIIa変異体のユニークな構造および物性解析2007

    • Author(s)
      藤田直子
    • Organizer
      日本農芸化学会平成19年度大会
    • Place of Presentation
      東京農業大学世田谷キャンパス
    • Year and Date
      2007-03-25
    • Related Report
      2007 Annual Research Report
  • [Presentation] 胚乳特異的RNAi法による高アミロース成分米の研究2007

    • Author(s)
      関川晶子
    • Organizer
      日本農芸化学会平成19年度大会
    • Place of Presentation
      東京農業大学世田谷キャンパス
    • Year and Date
      2007-03-25
    • Related Report
      2007 Annual Research Report
  • [Patent(Industrial Property Rights)] デンプン合成酵素類2009

    • Inventor(s)
      中村保典, 藤田直子, 佐藤光
    • Industrial Property Rights Holder
      (独)科学技術振興機構
    • Acquisition Date
      2009-05-15
    • Related Report
      2009 Final Research Report
  • [Patent(Industrial Property Rights)] イネSSIIaの活性の制御方法,及びその変異体2009

    • Inventor(s)
      中村保典, 藤田直子, ペリジオフランシスコジュニア
    • Industrial Property Rights Holder
      (独)科学技術振興機構
    • Acquisition Date
      2009-08-14
    • Related Report
      2009 Final Research Report
  • [Patent(Industrial Property Rights)] 特許2009

    • Inventor(s)
      藤田直子
    • Industrial Property Rights Holder
      秋田県立大学
    • Industrial Property Number
      2008-232298
    • Filing Date
      2009-09-10
    • Related Report
      2008 Annual Research Report
  • [Patent(Industrial Property Rights)] イネ変異体及びその製造方法,並びに該イネ変異体に由来する澱粉及びその製造方法2008

    • Inventor(s)
      藤田直子
    • Industrial Property Rights Holder
      秋田県立大学
    • Industrial Property Number
      2008-232298
    • Filing Date
      2008-09-10
    • Related Report
      2009 Final Research Report

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Published: 2007-04-01   Modified: 2016-04-21  

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