Molecular mechanism of orchid-specific floral organogenesis by a transgenic approach.

• Project/Area Number: 19380016
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Horticulture/Landscape architecture
• Research Institution: Tohoku University
• Principal Investigator: KANNO Akira Tohoku University, 大学院・生命科学研究科, 准教授 (10260449)
• Co-Investigator(Kenkyū-buntansha): MII Masahiro 千葉大学, 大学院・園芸学研究科, 教授 (30093074) ; HANDA Takashi 明治大学, 農学部, 教授 (00192708) ; YUKAWA Tomohisa 独立行政法人国立科学博物館, 筑波実験植物園, 研究主幹 (50280524)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000); Fiscal Year 2009: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2008: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000); Fiscal Year 2007: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
• Keywords: デンドロビウム / コチョウラン / セッコク / サギソウ / MADS-box遺伝子 / 遺伝子単離 / 遺伝子発現 / 形質転換 / ラン科植物 / 遺伝子解析 / 変異体

Research Abstract

In order to understand the molecular mechanism of the orchid-specific floral organs such as lip, column, pollinium, we isolated floral organ identity genes from Dendrobium, Habenaria and Phalaenopsis orchids. The results indicated that expression pattern of class B genes were related to the various morphology of the tepals. Also, to understand the function of the floral organ identity genes, we constructed the transgenic Phalaenopsis.

Research Products

• [Journal Article] Production of intraspecific hybrids between wild-type and petaloid-sepal cultivars in Habenaria radiata (2010)
  • Author(s): Kim, S. Y., M. Endo, A. Kanno
  • Journal Title: Scientia Horticulturae 124 Pages: 415-418
  • Peer Reviewed
• [Journal Article] (2009)
  • Author(s): Supatida Sirisawat, Hiroshi Ezura, Naoya Fukuda, Takahiro Kounosu, Takashi Handa
  • Journal Title: Acta Horticulturae 836巻 Pages: 259-264
  • Peer Reviewed
• [Journal Article] DMMADS4, a DEF-like gene from Dendrobium is required for floral organ identity and flower longevity of orchid (2009)
  • Author(s): Sirisawat, S., et al.
  • Journal Title: Acta Horticulturae 936 Pages: 259-364
  • Peer Reviewed
• [Journal Article] DMMADS4, a DEF-like gene from Dendrobium is required for floral organ identity and flower longevity of orchid. (2009)
  • Author(s): Sirisawat, S., et al.
  • Journal Title: Acta Horticulturae 836 Pages: 259-264
  • Peer Reviewed
• [Presentation] (2009)
  • Author(s): Supatida Sirisawat, Naoya Fukuda, Hiroshi Ezura, Takashi Handa
  • Organizer: 23rd EUCARPIA Symposium
  • Place of Presentation: ライデン(オランダ)
  • Year and Date: 2009-09-03
• [Presentation] DMMADS4, a DEF-Like Gene from Dendrobium is Required for Floral Organ Identity and Flower Longevity of Orchid (2009)
  • Author(s): Sirisawat, S., et al.
  • Organizer: 23^<rd> EUCARPIA Symposium
  • Place of Presentation: ライデン (オランダ)
  • Year and Date: 2009-09-03
• [Presentation] DMMADS4, a DEF-Like Gene from Dendrobium is Required for Floral Organ Identity and Flower Longevity of Orchid. (2009)
  • Author(s): Sirisawat, S., et al.
  • Organizer: 23^<rd> EUCARPIA Symposium
  • Place of Presentation: ライデン(オランダ)
  • Year and Date: 2009-09-03
• [Presentation] (2008)
  • Author(s): A.Kanno, M.Endo, S.Y.Kim
  • Organizer: 4^<th> International Symposium on Diversity and Conservation of Asian Orchids
  • Place of Presentation: 国立科学博物館筑波実験植物園
  • Year and Date: 2008-12-13
• [Presentation] (2008)
  • Author(s): S.Sirisawat, N.Fukuda, H.Ezura, T.Handa
  • Organizer: 4th International Symposium on Diversity and Conservation of Asian Orchids
  • Place of Presentation: 国立科学博物館筑波実験植物園
  • Year and Date: 2008-12-13
• [Presentation] Expression of class B and C MADS-box genes in Dendrobium moniliforme and its floral organ mutant cultivars (2008)
  • Author(s): S. Sirisawat, N. Fukuda, H. Ezura, T. Handa
  • Organizer: 4^<th> International Symposium on Diversity and Conservation of Asian Orchids
  • Place of Presentation: 国立科学博物館筑波実験植物園 (筑波)
  • Year and Date: 2008-12-13
• [Presentation] Production and characterization of intraspecific hybrids between wild-type and petaloid-sepal cultivar in Habenaria radiata (2008)
  • Author(s): A Kanno, M. Endo, S. Y. Kim
  • Organizer: 4^<th> International Symposium on Diversity and Conservation of Asian Orchids
  • Place of Presentation: 国立科学博物館筑波実験植物園 (筑波)
  • Year and Date: 2008-12-13
• [Presentation] Production and characterization of intraspecific hybrids between wild-type and petaloid-sepal cultivar in Habenaria radiata (2008)
  • Author(s): A. Kanno, M. Endo and S. Y. Kim
  • Organizer: 4^<th> International Symposium on Diversity and Conservation of Asian Orchids
  • Place of Presentation: 国立科学博物館筑波実験植物園
  • Year and Date: 2008-12-13
• [Presentation] Expression of class B and C MADS-box genes in Dendrobium moniliforme and its floral organ mutant cultivars (2008)
  • Author(s): S. Sirisawat, N. Fukuda, H. Ezura and T. Handa
  • Organizer: 4^<th> International Symposium on Diversity and Conservation of Asian Orchids
  • Place of Presentation: 国立科学博物館筑波実験植物園
  • Year and Date: 2008-12-13
• [Presentation] (2008)
  • Author(s): Supatida Sirisawat,福田直也,半田高
  • Organizer: 園芸学会平成20年度春季大会
  • Place of Presentation: 東京農業大学農学部(神奈川)
  • Year and Date: 2008-03-28
• [Presentation] Expression profiles of class B MADS-box genes in Dendrobium moniliforme and its petaloid-sepal mutants (2008)
  • Author(s): Supatida Sirisawat, 福田直也, 半田高
  • Organizer: 園芸学会平成20年度春季大会
  • Place of Presentation: 東京農業大学農学部 (神奈川)
  • Year and Date: 2008-03-28
• [Presentation] Expression profiles of class B MADS-box genes in Dendrobium moniliforme and its petaloid-sepal mutants. (2008)
  • Author(s): Supatida Sirisawat, 福田直也, 半田 高
  • Organizer: 園芸学会平成20年度春季大会
  • Place of Presentation: 東京農業大学農学部(神奈川)
  • Year and Date: 2008-03-28
• [Presentation] (2008)
  • Author(s): Supatida Sirisawat,福田直也,半田高
  • Organizer: 名古屋国際蘭会議2008
  • Place of Presentation: ナゴヤドーム(名古屋)
  • Year and Date: 2008-03-13
• [Presentation] Expression of class B and C MADS-box genes in Dendrobium moniliforme and its floral organ mutant cultivars (2008)
  • Author(s): Supatida Sirisawat, 福田直也, 半田高
  • Organizer: 名古屋国際蘭会議2008
  • Place of Presentation: ナゴヤドーム (名古屋)
  • Year and Date: 2008-03-13
• [Presentation] Expression of class B and C MADS-box genes in Dendrobium moniliforme and its floral organ mutant cultivars. (2008)
  • Author(s): Supatida Sirisawat, 福田直也, 半田 高
  • Organizer: 名古屋国際蘭会議2008
  • Place of Presentation: ナゴヤドーム(名古屋)
  • Year and Date: 2008-03-13
• [Presentation] (2008)
  • Author(s): 菅野明,金昭英,遠藤都子
  • Organizer: 園芸学会平成20年度秋季大会
  • Place of Presentation: 三重大学
• [Presentation] サギソウの獅子咲き品種'飛翔'と野生株の雑種作出と形質調査 (2008)
  • Author(s): 菅野明, 金昭英, 遠藤都子
  • Organizer: 園芸学会平成20年度秋季大会
  • Place of Presentation: 三重大学 (三重)
• [Presentation] サギソウの獅子咲き品種'飛翔'と野生株の雑種作出と形質調査 (2008)
  • Author(s): 菅野明, 金昭英, 遠藤都子
  • Organizer: 園芸学会平成20年度秋季大会
  • Place of Presentation: 三重大学
• [Book] Plant Breedingi (2009)
  • Author(s): A.Kanno, S.Y.Kim
  • Publisher: Nova Science Publishers, Inc.
• [Book] Orchidaceae(Morphological differentiation of sepal and petal in Habenaria radiata) (2009)
  • Author(s): A. Kanno, S. Y. Kim
  • Publisher: Plant Breeding Nova Science Publishers, Inc.
• [Book] Morphological differentiation of sepal and petal in Habenaria radiata(Orchidaceae). In : Plant Breeding pp.313-323 (2009)
  • Author(s): A.Kanno, S.Y. Kim
  • Total Pages: 343
  • Publisher: Nova Science Publishers, Inc.