Improvements in rooting of microcuttings of fruit trees by applying the findings in development of lateral roots of Arabidopsis thaliana

• Project/Area Number: 19380023
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Horticulture/Landscape architecture
• Research Institution: University of Miyazaki
• Principal Investigator: TETSUMURA Takuya University of Miyazaki, 農学部, 教授 (00227498)
• Co-Investigator(Kenkyū-buntansha): HONSHO Chitose 宮崎大学, 農学部, 助教 (30381057)
• Project Period (FY): 2007 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥20,410,000 (Direct Cost: ¥15,700,000、Indirect Cost: ¥4,710,000); Fiscal Year 2010: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2009: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2008: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2007: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
• Keywords: 果樹 / 発根 / ミクロ挿し穂 / 根発達培地 / 側根 / 挿し木

Research Abstract

We had shown that waving primary roots of Arabidopsis thaliana produced more lateral roots than straight roots, and then, by using vermiculite-containing and gellan gum-solidified medium as a root development medium, we were successful in inducing lateral roots from adventitious roots produced from microcuttings of kaki cultivars and mango seedlings. The root development medium also improved rooting of the microcuttings of Japanese pear cultivars of low-rooting-ability and mango seedlings. To analyze the genetic makeup of lateral roots, we produced mutated strains of Arabidopsis thaliana having many lateral roots and few lateral roots.

Research Products

• [Journal Article] Development of in vitro root system from microcuttings of fruit trees. (2009)
  • Author(s): Takuya Tetsumura, Kazumi Irishima, Chitose Honsho.
  • Journal Title: Combined Proceedings International Plant Propagators' Society. Vol.58 Pages: 474-477
• [Journal Article] Development of in vitro root system from microcuttings of fruit trees (2009)
  • Author(s): Takuya Tetsumura, Kazumi Irishima, Chitose Honsho
  • Journal Title: Combined Proceedings International Plant Propagators' Society Vol.58 Pages: 474-477
• [Journal Article] Development of in vitro root system from microcuttings of fruit trees (2009)
  • Author(s): Takuya Tetsumura
  • Journal Title: Combined Proceedings International Plant Propagators' Society Vol.58 Pages: 474-477
• [Journal Article] Evaluation of basal media for micropropagation of four highbush blueberry cultivars. (2008)
  • Author(s): Takuya Tetsumura, Yasuyo Matsumoto, Makiko Sato, Chitose Honsho, Kensuke Yamashita, Haruki Komatsu, Yasuhiro Sugimoto, Hisato Kunitake.
  • Journal Title: Scientia Horticulturae. Vol.119 Pages: 72-74
  • URL: http://hdl.handle.net/10458/2763
  • Peer Reviewed
• [Journal Article] Improvement of rooting of cuttings of a dwarfing rootstock for kaki and its micropropagation. (2008)
  • Author(s): T.Tetsumura, S.Haranoushiro, C.Honsho.
  • Journal Title: Acta Horticulturae. Vol.833 Pages: 177-182
  • URL: http://www.actahort.org/books/833/833_28.htm
  • Peer Reviewed
• [Journal Article] Evaluation of basal media for micropropagation of four highbush blueberry cultivars (2008)
  • Author(s): Takuya Tetsumura, Yasuyo Matsumoto, Makiko Sato, Chitose Honsho, Kensuke Yamashita, Haruki Komatsu, Yasuhiro Sugimoto, Hisato Kunitake
  • Journal Title: Scientia Horticulturae Vol.119 Pages: 72-74
  • URL: http://hdl.handle.net/10458/2763
  • Peer Reviewed
• [Journal Article] Evaluation of basal media for micropropagation of four highbush blueberry cultivars. (2008)
  • Author(s): Takuya Tetsumura
  • Journal Title: Scientia Horticulturae 119 Pages: 72-74
  • Peer Reviewed
• [Presentation] improves rooting of microcuttings of Japanese pear cultivars. (2010)
  • Organizer: 28th International Horticultural Congress
  • Place of Presentation: リスボン会議場(ポルガル、リスボン)【Invited speaker】
  • Year and Date: 2010-10-24
• [Presentation] マンゴーの試験管内繁殖に関する研究 (2010)
  • Author(s): 迫田剛行
  • Organizer: 国際植物増殖者会議日本支部第17回愛知大会
  • Place of Presentation: 愛知豊明花き地方卸売市場(愛知県)
  • Year and Date: 2010-10-24
• [Presentation] Vermiculite-containing and gellan gum-solidified medium improves rooting of microcuttings of Japanese pear cultivars (2010)
  • Author(s): T.Tetsumura
  • Organizer: 28th International Horticultural Congress
  • Place of Presentation: リスボン会議場(ポルトガル、リスボン)(Invited speaker)
  • Year and Date: 2010-08-25
• [Presentation] ニホンナシ栽培品種のミクロ繁殖における基本培地と発根培地の再検討 (2009)
  • Author(s): 鉄村琢哉・石村麻美・愛甲優瑞美・江口奈々・甲斐由利加・本勝千歳
  • Organizer: 園芸学会平成21年度秋季大会
  • Place of Presentation: 秋田大学手形キャンパス
  • Year and Date: 2009-09-27
• [Presentation] ニホンナシ栽培品種のミクロ繁殖における基本培地と発根培地の再検討 (2009)
  • Author(s): 鉄村琢哉
  • Organizer: 園芸学会
  • Place of Presentation: 秋田大学手形キャンパス(秋田市)
  • Year and Date: 2009-09-27
• [Presentation] Improvement of rooting of cuttings of a dwarfing rootstock for kaki and its micropropagation (2008)
  • Author(s): T. Tetsumura, S. Haranoushiro, C. Honsho
  • Organizer: IV International Symposium on Persimmon
  • Place of Presentation: Faenza, Italy
  • Year and Date: 2008-11-10
• [Presentation] Improvement of rooting of cuttings of a dwarfingrootstock for kaki and its micropropagation (2008)
  • Author(s): T. Tetsumura
  • Organizer: IV International Symposium on Persimmon
  • Place of Presentation: Faenza Fiere, Faenza, Italy
  • Year and Date: 2008-11-10
• [Presentation] 果樹ミクロ挿し穂のin vitroでの根系発達 (2008)
  • Author(s): 鉄村琢哉・入嶋和美・本勝千歳
  • Organizer: 国際植物増殖者会議日本支部第15回茨城大会
  • Place of Presentation: つくば国際会議場
  • Year and Date: 2008-10-18
• [Presentation] 果樹ミクロ挿し穂の in vitro での根系発達 (2008)
  • Author(s): 鉄村琢哉・入嶋和美・本勝千歳
  • Organizer: 国際植物増殖者会議日本支部第15回茨城大会
  • Place of Presentation: つくば国際会議場
  • Year and Date: 2008-10-18
• [Presentation] ブルーベリーのマイクロプロパゲーションにおける基本培地の再検討 (2007)
  • Author(s): 鉄村琢哉, ほか7名
  • Organizer: 園芸学会平成19年度秋季大会
  • Place of Presentation: 香川大学幸町キャンパス
  • Year and Date: 2007-09-29
• [Presentation] Vermiculite-containing and gellan gum-solidified medium
  • Author(s): Tetsumura, T., Ishimura, A., Aikou, Y., Eguchi, N., Kai, Y., Tashiro, K., Honsho, C.
• [Remarks] ホームページ等